##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8503873
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89636122270406	34.0	31.0	34.0	31.0	34.0
2	33.011249815231245	34.0	31.0	34.0	31.0	34.0
3	33.07232328140366	34.0	33.0	34.0	31.0	34.0
4	36.45193360719286	37.0	37.0	37.0	35.0	37.0
5	36.4093637099237	37.0	37.0	37.0	35.0	37.0
6	36.438545942537004	37.0	37.0	37.0	35.0	37.0
7	36.39305031954264	37.0	37.0	37.0	35.0	37.0
8	36.374756302216646	37.0	37.0	37.0	35.0	37.0
9	38.15597986940774	39.0	39.0	39.0	37.0	39.0
10-11	38.104738629092886	39.0	38.5	39.0	37.0	39.0
12-13	38.074497878790055	39.0	38.0	39.0	37.0	39.0
14-15	39.52235369695667	41.0	39.5	41.0	37.0	41.0
16-17	39.49833528793292	41.0	39.0	41.0	37.0	41.0
18-19	39.502460173146986	41.0	39.5	41.0	37.0	41.0
20-21	39.477754069130285	41.0	39.0	41.0	37.0	41.0
22-23	39.39511689720918	41.0	39.0	41.0	36.0	41.0
24-25	39.23687104673356	40.5	39.0	41.0	36.0	41.0
26-27	39.243992085551895	40.5	39.0	41.0	36.0	41.0
28-29	39.176083351018704	41.0	39.0	41.0	36.0	41.0
30-31	39.0919341599033	40.0	39.0	41.0	36.0	41.0
32-33	38.89202277389448	40.0	38.5	41.0	35.0	41.0
34-35	38.80697739541294	40.0	38.0	41.0	35.0	41.0
36-37	38.9973612658833	40.0	39.0	41.0	35.0	41.0
38-39	39.062241089310334	41.0	39.0	41.0	35.0	41.0
40-41	39.04145129343263	40.5	39.0	41.0	35.0	41.0
42-43	38.89649535344489	40.0	39.0	41.0	35.0	41.0
44-45	38.915878617042736	40.0	38.0	41.0	35.0	41.0
46-47	38.866008320615286	40.0	38.0	41.0	35.0	41.0
48-49	38.80078144871504	40.0	38.0	41.0	35.0	41.0
50-51	38.72568567056544	40.0	38.0	41.0	35.0	41.0
52-53	38.61271839063609	40.0	38.0	41.0	35.0	41.0
54-55	38.47387174126596	40.0	38.0	41.0	34.0	41.0
56-57	38.32140429016131	40.0	37.5	41.0	34.0	41.0
58-59	38.16072910728583	40.0	37.0	41.0	34.0	41.0
60-61	37.97068857875797	40.0	36.5	41.0	34.0	41.0
62-63	37.743443343358095	39.0	36.0	41.0	33.5	41.0
64-65	37.47499143190703	39.0	35.5	41.0	33.0	41.0
66-67	37.148945434462405	38.5	35.0	40.0	33.0	41.0
68-69	36.81679517353065	38.0	35.0	40.0	32.0	41.0
70-71	36.45850795323045	37.0	35.0	40.0	32.0	41.0
72-73	36.11491875791589	37.0	35.0	39.0	32.0	41.0
74-75	35.77197162410745	36.0	35.0	39.0	31.0	40.5
76-77	34.91347624207408	35.0	34.0	37.0	30.5	39.0
78-79	35.14461844919522	35.0	34.0	37.0	31.0	39.0
80-81	34.92977824948338	35.0	34.0	37.0	31.0	39.0
82-83	34.66099203394401	35.0	34.0	36.5	31.0	39.0
84-85	34.39906979304915	35.0	34.0	36.0	31.0	37.0
86-87	34.17949032096618	35.0	34.0	36.0	31.0	37.0
88-89	33.979881939214124	35.0	34.0	35.0	31.0	37.0
90-91	33.783178109483984	35.0	34.0	35.0	30.0	36.0
92-93	33.65272629527222	35.0	34.0	35.0	30.0	36.0
94-95	33.5784070760474	35.0	34.0	35.0	30.0	36.0
96-97	33.557167599753704	35.0	34.0	35.0	30.0	35.5
98-99	33.59198818694577	35.0	34.0	35.0	30.5	35.0
100-101	32.188113154959574	33.5	31.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	3.0
20	16.0
21	155.0
22	695.0
23	2464.0
24	6526.0
25	13401.0
26	24411.0
27	39260.0
28	56736.0
29	77063.0
30	101115.0
31	129920.0
32	166243.0
33	220511.0
34	315817.0
35	478189.0
36	856459.0
37	1882493.0
38	2736371.0
39	1324506.0
40	71516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.411568470037125	21.346156039724487	15.912667087102546	24.32960840313584
2	13.286803029096458	21.382516059389186	41.95550786675109	23.375173044763265
3	16.82475737819697	26.18254059062265	31.189788464620765	25.802913566559617
4	11.700068898018586	18.82887949996431	38.84923963469351	30.62181196732359
5	13.054722242441768	37.74620105450775	35.66663095744727	13.532445745603209
6	29.687731695899032	38.291105711479936	18.65856886620955	13.362593726411484
7	26.32371218324271	32.80717282921456	22.668376006262562	18.200738981280168
8	24.26729148353829	37.24586289111077	20.78672832519484	17.70011730015611
9	24.97403525936518	17.39919847974562	21.23118497055448	36.395581290334725
10-11	23.934976451318125	27.253540827808692	28.57482702293414	20.236655697939046
12-13	24.851464738478573	24.53723144736522	28.905699791142226	21.705604023013983
14-15	21.88100143243546	26.0689260050244	26.62306132895814	25.427011233581997
16-17	21.467439460189112	29.37425408934547	27.228500869075212	21.92980558139021
18-19	21.451860816830166	28.259982245736737	29.17497709573038	21.113179841702717
20-21	22.201224697917883	27.27312442848058	29.250105366543867	21.27554550705768
22-23	21.85705549250554	27.34155204958279	29.122410457547087	21.67898200036458
24-25	21.726438326130058	27.544671128839354	29.15977979902344	21.569110746007144
26-27	21.641268495121636	27.620244583324087	29.139980999249772	21.5985059223045
28-29	21.438070646261828	27.579956528374783	29.188949835232325	21.79302299013106
30-31	21.750805969436545	27.397157377961623	29.207962424280893	21.644074228320942
32-33	21.618678616963088	27.593209013789664	29.125845307029703	21.66226706221755
34-35	21.67567949286021	27.767396568918073	29.16640207183828	21.390521866383434
36-37	21.74363726351302	27.61508123477403	28.934860712161566	21.706420789551387
38-39	21.633757744578542	27.656878217737933	29.0646353916146	21.644728646068923
40-41	21.842291644837204	27.555329246357346	28.962600837110518	21.639778271694933
42-43	21.918701208885828	27.56585605209356	28.817405171762182	21.698037567258424
44-45	21.815936224702305	27.798597169493743	28.583189174100347	21.802277431703597
46-47	22.05953959318671	27.702535295184695	28.183491945977806	22.05443316565079
48-49	21.9349883901862	27.8077124473185	28.21276808621284	22.04453107628246
50-51	21.866561628401314	28.001816720513357	28.09112215290127	22.04049949818406
52-53	22.014404608186407	27.972021330021548	28.10125168197596	21.91232237981609
54-55	21.99843600968375	27.902641887681746	28.01280362767763	22.08611847495688
56-57	22.105675577127528	28.072068686580327	27.840376422277792	21.981879314014353
58-59	21.9156841841718	28.201545210371727	28.05196725226191	21.830803353194568
60-61	22.101280162372362	28.267925542355222	27.814677936361953	21.816116358910467
62-63	21.92954664641786	28.411642418704503	27.786331638122974	21.87247929675466
64-65	21.927536019947176	28.32024253644087	27.78208582445803	21.97013561915392
66-67	22.050291318662627	28.28082424875612	27.65249861392401	22.01638581865724
68-69	22.022197359456065	28.16515068546127	27.76463247045629	22.04801948462637
70-71	21.994215049449057	27.975902159793016	27.805067858428	22.224814932329927
72-73	22.039421646022475	27.97322379102481	27.76787638044882	22.219478182503895
74-75	21.92842573987743	28.06371570339457	27.779188405745174	22.228670150982826
76-77	22.063994870115042	28.04205082549705	27.7085985749671	22.185355729420806
78-79	22.028754751702284	28.05351614227632	27.639943060536137	22.27778604548526
80-81	22.024058459713416	28.069844712317337	27.672592544158903	22.233504283810344
82-83	22.07926559635679	28.01637238158392	27.737028462899673	22.167333559159616
84-85	22.170341585819216	28.03995279457569	27.702653721841582	22.087051897763512
86-87	21.99539874596815	28.055571703118147	27.76785127614066	22.181178274773046
88-89	22.055732712351496	28.02783314924718	27.737077405939537	22.17935673246179
90-91	22.044656949222404	28.080521004943414	27.7669521110969	22.107869934737288
92-93	22.014169490543107	28.01437113131946	27.8272723376022	22.14418704053523
94-95	21.892462839147147	28.14967403592773	27.81435386376325	22.143509261161874
96-97	21.871016437894045	28.166894171838237	27.864395449730516	22.0976939405372
98-99	22.08426997816737	28.699261286998112	27.884859350862072	21.331609383972445
100-101	19.917670699525587	32.50243464792261	26.018607542084897	21.561287110466903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2850.0
1	2273.0
2	2300.5
3	3079.5
4	4052.0
5	5928.5
6	7528.0
7	9419.5
8	11167.0
9	11512.0
10	10548.5
11	9618.5
12	10680.5
13	14689.0
14	24806.5
15	38699.5
16	49591.0
17	54856.5
18	54650.5
19	50769.0
20	45406.0
21	40155.0
22	37373.0
23	39317.0
24	45790.5
25	56336.0
26	70443.0
27	86554.5
28	104056.0
29	122661.5
30	141495.5
31	161448.5
32	182190.5
33	202713.5
34	222420.0
35	240669.0
36	261090.0
37	278822.5
38	291764.0
39	304103.0
40	317250.0
41	328257.5
42	334734.0
43	341358.5
44	348327.5
45	355411.5
46	361856.0
47	365239.5
48	360508.0
49	350717.5
50	340859.5
51	326254.5
52	307129.5
53	287411.5
54	268242.5
55	247542.5
56	225198.5
57	201509.0
58	177308.5
59	152731.5
60	126547.0
61	101877.0
62	79827.5
63	60633.5
64	45032.0
65	33042.5
66	24186.5
67	18077.0
68	13578.5
69	9630.0
70	6640.5
71	4569.0
72	3186.5
73	2140.5
74	1400.0
75	914.0
76	573.0
77	361.5
78	230.0
79	160.5
80	116.5
81	82.5
82	65.5
83	49.5
84	36.5
85	29.0
86	25.0
87	20.0
88	12.5
89	7.5
90	4.5
91	2.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.028927995514514387
3	0.0
4	0.0
5	0.0
6	0.0
7	4.703739108051119E-5
8	5.644486929661344E-4
9	3.8805847641421737E-4
10-11	0.0
12-13	0.0
14-15	0.002046126512002237
16-17	7.05560866207668E-5
18-19	0.0
20-21	1.2347626612710283E-4
22-23	0.0020521868167401288
24-25	7.233805230323477E-4
26-27	0.014636448675157256
28-29	0.31047245667879225
30-31	0.31192058005386164
32-33	0.3296042594648791
34-35	0.2122029380743724
36-37	0.07066311514245832
38-39	0.061929002481099296
40-41	0.024547053140978246
42-43	0.009400628380050007
44-45	0.012433253460944057
46-47	0.00885404400318549
48-49	0.006173831668991875
50-51	0.006540515714978943
52-53	0.0025600100639216273
54-55	0.0011647812930047224
56-57	0.0028955113897236513
58-59	0.0034838173150477064
60-61	0.002948296979427626
62-63	0.0021948498892515327
64-65	7.114154915466054E-4
66-67	0.00870336080709742
68-69	0.01278186221528469
70-71	0.012715761474264167
72-73	0.010529352431843112
74-75	0.010575720158314742
76-77	0.009525902568846186
78-79	0.0024113240825875694
80-81	0.0019671867587668598
82-83	0.0015861412976107638
84-85	7.21310141577312E-6
86-87	0.002819066416986252
88-89	0.0
90-91	0.009215848278975721
92-93	0.019194448820537335
94-95	0.0014229025498413695
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	137.0
20-21	415.0
22-23	991.0
24-25	2298.0
26-27	3608.0
28-29	6242.0
30-31	14306.0
32-33	18909.0
34-35	27585.0
36-37	39997.0
38-39	48145.0
40-41	51027.0
42-43	60773.0
44-45	73165.0
46-47	73339.0
48-49	64483.0
50-51	56376.0
52-53	49683.0
54-55	57009.0
56-57	61027.0
58-59	62207.0
60-61	62907.0
62-63	62500.0
64-65	67588.0
66-67	73498.0
68-69	68751.0
70-71	64055.0
72-73	60548.0
74-75	60915.0
76-77	62098.0
78-79	66288.0
80-81	68250.0
82-83	66486.0
84-85	66767.0
86-87	71663.0
88-89	78034.0
90-91	83309.0
92-93	88406.0
94-95	103276.0
96-97	171047.0
98-99	765399.0
100-101	5520366.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.48501957276152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.80663971642302	76.77122353922364
2	7.171140594660759	12.260501881871992
3	1.7261947130371313	4.426913664911298
4	0.614198052416572	2.100189301295307
5	0.2743432334602298	1.1726118341001215
6	0.14455582177167914	0.7414414352105172
7	0.0771639960286241	0.4617455997573596
8	0.05125642395040247	0.3505325123703934
9	0.033752950560878237	0.25968344754016154
>10	0.09869064680402748	1.2569296219293988
>50	0.0015133478691539304	0.08340255295748732
>100	5.076299800741171E-4	0.08044689017069223
>500	2.1436530831461774E-5	0.013468891594092173
>1k	2.1436506540659714E-5	0.02090882706749116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.17593477701278E-5	0.0	0.0	0.0
3	0.0	1.17593477701278E-5	0.0	0.0	0.0
4	0.0	2.35186955402556E-5	0.0	0.0	0.0
5	0.0	2.35186955402556E-5	0.0	0.0	0.0
6	0.0	2.35186955402556E-5	0.0	0.0	0.0
7	0.0	2.35186955402556E-5	0.0	0.0	0.0
8	0.0	2.35186955402556E-5	0.0	0.0	1.17593477701278E-5
9	0.0	1.293528254714058E-4	0.0	1.17593477701278E-5	1.17593477701278E-5
10-11	0.0	1.293528254714058E-4	0.0	2.93983694253195E-5	1.17593477701278E-5
12-13	0.0	1.293528254714058E-4	0.0	3.52780433103834E-5	1.17593477701278E-5
14-15	0.0	1.5875119489672528E-4	0.0	3.52780433103834E-5	2.35186955402556E-5
16-17	0.0	1.6463086878178919E-4	0.0	3.52780433103834E-5	2.35186955402556E-5
18-19	0.0	1.881495643220448E-4	0.0	3.52780433103834E-5	3.52780433103834E-5
20-21	0.0	2.1166825986230039E-4	0.0	4.11577171954473E-5	3.52780433103834E-5
22-23	0.0	2.3518695540255597E-4	0.0	5.2917064965575097E-5	4.11577171954473E-5
24-25	0.0	2.587056509428116E-4	0.0	7.64357605058307E-5	4.70373910805112E-5
26-27	0.0	2.704649987129394E-4	0.0	1.293528254714058E-4	4.70373910805112E-5
28-29	0.0	2.763446725980033E-4	0.0	3.9981782418434513E-4	4.70373910805112E-5
30-31	0.0	2.822243464830672E-4	0.0	0.0015110761884614223	4.70373910805112E-5
32-33	0.0	2.9398369425319497E-4	0.0	0.003692435199820129	5.8796738850639E-5
34-35	0.0	3.1162271590838667E-4	0.0	0.006644031490122207	5.8796738850639E-5
36-37	0.0	3.2926173756357837E-4	0.0	0.011906339617254397	7.05560866207668E-5
38-39	0.0	3.2926173756357837E-4	0.0	0.02041422772894186	7.05560866207668E-5
40-41	0.0	3.645397808739618E-4	0.0	0.03246167951943779	7.05560866207668E-5
42-43	0.0	3.821788025291535E-4	0.0	0.04026988643880265	7.05560866207668E-5
44-45	0.0	4.409755413797925E-4	0.0	0.05082390106249235	7.05560866207668E-5
46-47	0.0	4.938926063453676E-4	0.0	0.06333584708990833	7.05560866207668E-5
48-49	0.0	4.938926063453676E-4	0.0	0.07461306160146089	7.05560866207668E-5
50-51	0.0	5.820877146213261E-4	0.0	0.08642532643655426	7.05560866207668E-5
52-53	0.0	5.879673885063899E-4	0.0	0.0983081473582684	7.64357605058307E-5
54-55	0.0	5.938470623914539E-4	0.0	0.14536905713431986	8.231543439089459E-5
56-57	0.0	6.114860840466456E-4	0.0	0.16752366833324062	8.231543439089459E-5
58-59	0.0	6.114860840466456E-4	0.0	0.1928533034300959	8.231543439089459E-5
60-61	0.0	6.40884453471965E-4	0.0	0.20635303467020263	8.231543439089459E-5
62-63	0.0	6.46764127357029E-4	0.0	0.2144081878927402	8.231543439089459E-5
64-65	0.0	7.290795617479236E-4	0.0	0.22184597535734601	8.231543439089459E-5
66-67	0.0	7.408389095180513E-4	0.0	0.22729643304880026	8.231543439089459E-5
68-69	0.0	7.64357605058307E-4	0.0	0.23248818508931168	8.231543439089459E-5
70-71	0.0	7.64357605058307E-4	0.0	0.23755058430435166	1.0583412993115019E-4
72-73	0.0	7.64357605058307E-4	0.0	0.2412959365691374	1.411121732415336E-4
74-75	0.0	8.055153222537542E-4	0.0	0.24444744177153163	1.411121732415336E-4
76-77	0.0	8.23154343908946E-4	0.0	0.24687574708606302	1.411121732415336E-4
78-79	0.0	8.349136916790738E-4	0.0	0.24918057924900805	1.411121732415336E-4
80-81	0.0	8.466730394492015E-4	0.0	0.25016836446169877	1.411121732415336E-4
82-83	0.0	8.466730394492015E-4	0.0	0.2506681367419292	1.411121732415336E-4
84-85	0.0	8.466730394492015E-4	0.0	0.2510503155444584	1.469918471265975E-4
86-87	0.0	8.701917349894572E-4	0.0	0.2510914732616538	1.528715210116614E-4
88-89	0.0	9.113494521849044E-4	0.0	0.251109112283309	1.6463086878178919E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11200	0.0	50.120735	1
GTATCAA	20825	0.0	38.1855	1
TCAACGC	25730	0.0	30.392435	4
ATCAACG	25865	0.0	30.20045	3
CAACGCA	26545	0.0	29.459309	5
TATCAAC	26815	0.0	29.41434	2
AACGCAG	27370	0.0	28.60285	6
ACGCAGA	31425	0.0	24.91201	7
CGCAGAG	31510	0.0	24.885878	8
CTTATAC	7675	0.0	23.443142	1
GCAGAGT	36765	0.0	21.282265	9
TGGTATC	5345	0.0	21.069464	2
GTGGTAT	5905	0.0	20.67877	1
GTACATG	24910	0.0	19.62521	1
AGAGTAC	33310	0.0	19.384844	10-11
GAGTACT	22270	0.0	19.300663	12-13
TACATGG	24745	0.0	19.26795	2
TATACAC	11620	0.0	18.522598	3
CAGAGTA	35835	0.0	18.512457	10-11
ACATGGG	24635	0.0	18.436705	3
>>END_MODULE