FastQCFastQC Report
Fri 27 May 2016
SRR2140400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6008247
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84050.052.2880251
GTATCAA153900.037.050491
TCAACGC190850.029.305894
ATCAACG191400.029.2441373
CAACGCA195750.028.5723065
TATCAAC198100.028.4801352
AACGCAG201500.027.8636426
ACGCAGA233800.023.9774427
CGCAGAG236150.023.7206338
TGGTATC37000.022.7796482
GTGGTAT40050.022.0110321
GCAGAGT270900.020.6157349
TACATGG183300.019.4718992
GTACATG187150.019.392851
GAGTACT163700.019.17208312-13
AGAGTAC247350.018.98910910-11
CAGAGTA265350.018.55970610-11
ACATGGG183950.018.532893
GTACTTT175900.017.83050214-15
AGTACTT175800.017.15551812-13
Produced by FastQC (version 0.11.5)