##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140400_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6008247
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93564628751115	34.0	31.0	34.0	31.0	34.0
2	33.0459611597193	34.0	33.0	34.0	31.0	34.0
3	33.106916876087155	34.0	33.0	34.0	31.0	34.0
4	36.47212622916468	37.0	37.0	37.0	35.0	37.0
5	36.43171843634258	37.0	37.0	37.0	35.0	37.0
6	36.46345215168418	37.0	37.0	37.0	35.0	37.0
7	36.421457539944676	37.0	37.0	37.0	35.0	37.0
8	36.406418710815316	37.0	37.0	37.0	35.0	37.0
9	38.194804574445754	39.0	39.0	39.0	37.0	39.0
10-11	38.14815061697696	39.0	39.0	39.0	37.0	39.0
12-13	38.11642630537659	39.0	39.0	39.0	37.0	39.0
14-15	39.57823796192134	41.0	40.0	41.0	37.0	41.0
16-17	39.55618993360293	41.0	40.0	41.0	37.0	41.0
18-19	39.5650462605815	41.0	40.0	41.0	37.0	41.0
20-21	39.539390133626945	41.0	39.5	41.0	37.0	41.0
22-23	39.45962836858894	41.0	39.0	41.0	36.5	41.0
24-25	39.302503118900574	41.0	39.0	41.0	36.0	41.0
26-27	39.306102179704126	40.5	39.0	41.0	36.0	41.0
28-29	39.2346331509321	41.0	39.0	41.0	36.0	41.0
30-31	39.14927616992359	41.0	39.0	41.0	36.0	41.0
32-33	38.94922646107672	40.0	39.0	41.0	35.0	41.0
34-35	38.86735120098352	40.0	38.0	41.0	35.0	41.0
36-37	39.05460237369205	40.5	39.0	41.0	35.0	41.0
38-39	39.12354148530695	41.0	39.0	41.0	35.0	41.0
40-41	39.10045929634734	41.0	39.0	41.0	35.0	41.0
42-43	38.955309004993964	40.5	39.0	41.0	35.0	41.0
44-45	38.975471590379286	40.0	39.0	41.0	35.0	41.0
46-47	38.925918990630656	40.0	38.5	41.0	35.0	41.0
48-49	38.857856593438086	40.0	38.0	41.0	35.0	41.0
50-51	38.78178027538516	40.0	38.0	41.0	35.0	41.0
52-53	38.668933034202894	40.0	38.0	41.0	35.0	41.0
54-55	38.53035748465098	40.0	38.0	41.0	34.5	41.0
56-57	38.37986809406965	40.0	37.5	41.0	34.0	41.0
58-59	38.21788411995847	40.0	37.0	41.0	34.0	41.0
60-61	38.02894431379647	40.0	36.5	41.0	34.0	41.0
62-63	37.801059515449275	39.0	36.0	41.0	34.0	41.0
64-65	37.5282353582832	39.0	35.5	41.0	33.0	41.0
66-67	37.20661141889822	38.5	35.0	40.0	33.0	41.0
68-69	36.87631321251914	38.0	35.0	40.0	33.0	41.0
70-71	36.517650462233206	37.0	35.0	40.0	32.0	41.0
72-73	36.17726697222109	37.0	35.0	39.0	32.0	41.0
74-75	35.83810230363933	36.0	35.0	39.0	32.0	40.5
76-77	34.97839115479027	35.0	34.0	37.0	30.5	39.0
78-79	35.20859756499809	35.0	34.0	37.0	31.0	39.0
80-81	34.99413519348946	35.0	34.0	37.0	31.0	39.0
82-83	34.7295721405594	35.0	34.0	36.5	31.0	39.0
84-85	34.46849640934777	35.0	34.0	36.0	31.0	37.0
86-87	34.253115526954	35.0	34.0	36.0	31.0	37.0
88-89	34.05630983964653	35.0	34.0	35.0	31.0	37.0
90-91	33.858117027958954	35.0	34.0	35.0	31.0	36.0
92-93	33.729464763655166	35.0	34.0	35.0	31.0	36.0
94-95	33.65440581665931	35.0	34.0	35.0	30.5	36.0
96-97	33.631085171762514	35.0	34.0	35.0	31.0	35.5
98-99	33.65993146310629	35.0	34.0	35.0	31.0	35.0
100-101	32.24807832294302	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	20.0
21	88.0
22	511.0
23	1754.0
24	4431.0
25	9174.0
26	16355.0
27	26289.0
28	37799.0
29	51767.0
30	67591.0
31	86109.0
32	111031.0
33	146761.0
34	215471.0
35	325513.0
36	590859.0
37	1324628.0
38	1952628.0
39	983158.0
40	56307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.052955046621754	21.073967165464403	15.608229821443759	24.26484796647009
2	13.44225029268429	21.38151980064171	41.773506977140656	23.402722929533343
3	16.923455377250633	26.31125185099747	31.112252875089858	25.65303989666204
4	11.786499456497044	18.618741872629403	38.91256467984755	30.682193991026
5	13.046317836134232	37.81981666199808	35.65364406623097	13.480221435636716
6	29.725991624512112	38.27562348884791	18.650598086263763	13.347786800376216
7	26.298486928707774	32.896322602131775	22.830950745500804	17.974239723659647
8	24.426588498032775	36.92297885209348	20.907968051034192	17.742464598839554
9	25.213823728159092	17.173871795639972	21.278448525520037	36.3338559506809
10-11	23.99819781044288	27.18430184378239	28.61204774038085	20.205452605393887
12-13	24.8605375245059	24.52811943317244	28.971020998304496	21.640322044017164
14-15	21.917128430942412	25.981846888442234	26.76938993854313	25.33163474207222
16-17	21.412913774217042	29.245643922387632	27.390759046300307	21.950683257095022
18-19	21.410837470563376	28.18303741507298	29.365154262133363	21.040970852230277
20-21	22.232943859123516	27.277741715350945	29.335222449692917	21.154091975832625
22-23	21.891339417444406	27.23812800602835	29.249649881887414	21.62088269463983
24-25	21.7109527650929	27.436864725531795	29.26883923559834	21.583343273776965
26-27	21.62629314013424	27.547617853949095	29.27164091698503	21.554448088931633
28-29	21.492801884785862	27.52809481763284	29.27447194111966	21.704631356461633
30-31	21.80273053880994	27.411521525483522	29.20750724574389	21.578240689962644
32-33	21.648116905290955	27.55893602556184	29.166008172565057	21.62693889658215
34-35	21.713643673027306	27.694178105800514	29.17311853716122	21.419059684010957
36-37	21.751482503516602	27.57994837237111	29.031050566776823	21.637518557335465
38-39	21.601460809336643	27.679605274125578	29.10141151599165	21.617522400546125
40-41	21.846246894538005	27.562671625475986	28.985960320049514	21.605121159936495
42-43	21.882422609523488	27.609057421332977	28.781022245510407	21.727497723633128
44-45	21.823693608831775	27.80494841638496	28.602722592141983	21.768635382641282
46-47	21.991803749709952	27.7363642095036	28.20682412784804	22.065007912938405
48-49	21.88228301819714	27.902444999737764	28.21193752351774	22.00333445854736
50-51	21.861347181718088	28.049020556735798	28.07039177641854	22.019240485127575
52-53	22.054095186490404	27.96753327347121	28.004124285056758	21.974247254981627
54-55	22.022330123375475	27.88322063502958	28.01302705973695	22.08142218185799
56-57	22.06832194477122	28.064652137498204	27.840667161207993	22.026358756522583
58-59	21.895433198194105	28.146976382405203	28.054581797307243	21.903008622093445
60-61	22.095042928194296	28.13727403340389	27.902736632077556	21.864946406324254
62-63	21.98884166635005	28.30287112643916	27.82214999550854	21.88613721170225
64-65	21.923961859054018	28.243567788030667	27.81643206021044	22.016038292704884
66-67	22.048082319305976	28.22151964865648	27.672453206283826	22.057944825753715
68-69	21.982325410609164	28.15321325402303	27.766565499979805	22.097895835387998
70-71	22.01225163230301	27.911728576435568	27.830201361620126	22.24581842964129
72-73	22.025345894760566	27.972704690272966	27.79273625621127	22.2092131587552
74-75	21.922839408406894	28.07714588129911	27.717979343020616	22.28203536727338
76-77	22.03953402092454	28.04664737453934	27.6965595706265	22.217259033909624
78-79	21.997251775103262	28.05948203489259	27.647218345888923	22.29604784411522
80-81	22.003534509157067	28.059378624538105	27.631363621376572	22.305723244928256
82-83	22.020347164584937	28.059860176892016	27.70803142710206	22.211761231420994
84-85	22.16889725505941	28.02195886656444	27.67067706025319	22.138466818122964
86-87	21.966635084482345	28.083096466950906	27.738144214226946	22.212124234339807
88-89	22.007902101163197	28.064327615593804	27.741431268020666	22.18633901522233
90-91	21.988588034818264	28.063668700272782	27.796011733659576	22.15173153124938
92-93	22.026099870017564	28.012550251080405	27.8022845463053	22.159065332596732
94-95	21.875516550242473	28.209194960063638	27.778584484746798	22.13670400494709
96-97	21.844838301722373	28.188847103804925	27.87220984591357	22.094104748559136
98-99	22.05338299450745	28.704848249387105	27.846802243148094	21.394966512957353
100-101	19.89436530170539	32.521901263948884	25.98068807112272	21.603045363223007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2017.0
1	1646.5
2	1833.5
3	2549.0
4	3374.0
5	4913.5
6	6280.0
7	7768.5
8	9039.5
9	9243.5
10	8548.0
11	7865.5
12	8597.5
13	11484.0
14	18835.5
15	28580.5
16	35951.5
17	39747.5
18	39675.0
19	36695.0
20	32673.5
21	29170.5
22	27473.5
23	28988.0
24	33651.0
25	41498.5
26	52302.5
27	64247.5
28	77196.0
29	90475.5
30	102539.0
31	115695.0
32	129917.5
33	144394.0
34	158792.5
35	171386.5
36	185157.0
37	197425.5
38	205834.0
39	213105.0
40	221810.5
41	229436.0
42	233031.5
43	237073.0
44	241633.0
45	246592.5
46	251101.0
47	251949.5
48	248115.5
49	242243.0
50	235456.0
51	226011.5
52	214450.5
53	202391.0
54	190237.5
55	175969.0
56	161118.0
57	146178.5
58	130367.0
59	113621.5
60	95001.0
61	76643.5
62	60223.0
63	46028.5
64	34411.0
65	25650.5
66	19186.0
67	14487.0
68	10921.0
69	7765.5
70	5435.0
71	3820.5
72	2634.5
73	1772.0
74	1160.5
75	768.5
76	511.5
77	331.5
78	208.0
79	133.0
80	85.0
81	58.0
82	52.0
83	43.5
84	31.5
85	21.0
86	14.0
87	10.0
88	5.5
89	2.0
90	1.0
91	2.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.029542726855270766
3	0.0
4	0.0
5	0.0
6	0.0
7	6.657515911046933E-5
8	6.657515911046932E-4
9	4.49382323995668E-4
10-11	0.0
12-13	0.0
14-15	0.00218033646086787
16-17	5.8253264221660663E-5
18-19	0.0
20-21	9.986508227384803E-5
22-23	0.00216387328657647
24-25	7.491694831970858E-4
26-27	0.014938074437240937
28-29	0.31584088104534824
30-31	0.3161759720438793
32-33	0.3346142456343474
34-35	0.2167453163355538
36-37	0.07457351745180962
38-39	0.06594609688833028
40-41	0.02725433235820174
42-43	0.011950140416291494
44-45	0.01532135682274097
46-47	0.011735704608304292
48-49	0.008209633745624719
50-51	0.008320687455552405
52-53	0.004065502664110493
54-55	0.0019520058434239441
56-57	0.004396468249068901
58-59	0.005308911487652943
60-61	0.004523595803540326
62-63	0.0028610769706805565
64-65	0.0013114794640582963
66-67	0.008736490704856098
68-69	0.012390898283803312
70-71	0.012670768719639538
72-73	0.010898035028870557
74-75	0.010855018529036144
76-77	0.009645133288322711
78-79	0.0025360827665587733
80-81	0.0021073111395491684
82-83	0.0017413936709607125
84-85	1.0284026295021153E-5
86-87	0.003199671555792507
88-89	0.0
90-91	0.008982397477360106
92-93	0.019488182194046307
94-95	0.001419298883819687
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	76.0
20-21	292.0
22-23	718.0
24-25	1840.0
26-27	2739.0
28-29	4707.0
30-31	10933.0
32-33	14518.0
34-35	21006.0
36-37	29856.0
38-39	36234.0
40-41	38149.0
42-43	45012.0
44-45	53738.0
46-47	53738.0
48-49	47548.0
50-51	41622.0
52-53	37046.0
54-55	41463.0
56-57	43973.0
58-59	44566.0
60-61	45212.0
62-63	44211.0
64-65	47819.0
66-67	51107.0
68-69	48955.0
70-71	46886.0
72-73	44249.0
74-75	44388.0
76-77	45913.0
78-79	48508.0
80-81	49281.0
82-83	48190.0
84-85	48112.0
86-87	51445.0
88-89	56083.0
90-91	59099.0
92-93	62228.0
94-95	72435.0
96-97	118677.0
98-99	531070.0
100-101	3874605.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.25130053684585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.29953140076891	77.8845202118407
2	6.8921006072999695	11.889052816208148
3	1.6420625818117085	4.248900997324521
4	0.5555445503103422	1.916657598816968
5	0.24580520719419638	1.0600509399614149
6	0.13235821536059872	0.6849640926952538
7	0.07398257359622532	0.4466765232816142
8	0.044372898561457	0.3061776167612162
9	0.030195289920823785	0.2343944723182348
>10	0.08153400653337045	1.053630883947153
>50	0.0017094122072378907	0.0940856517774602
>100	7.452935468693941E-4	0.13226927804451136
>500	2.8981444174286997E-5	0.02069741044215709
>1k	2.898144415544953E-5	0.0279215065807518
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.664378977761733E-5
2	0.0	0.0	0.0	0.0	1.664378977761733E-5
3	0.0	0.0	0.0	0.0	1.664378977761733E-5
4	0.0	3.328757955523466E-5	0.0	1.664378977761733E-5	1.664378977761733E-5
5	0.0	3.328757955523466E-5	0.0	1.664378977761733E-5	1.664378977761733E-5
6	0.0	3.328757955523466E-5	0.0	1.664378977761733E-5	4.9931369332851994E-5
7	0.0	3.328757955523466E-5	0.0	3.328757955523466E-5	4.9931369332851994E-5
8	0.0	4.9931369332851994E-5	0.0	3.328757955523466E-5	4.9931369332851994E-5
9	0.0	9.986273866570399E-5	0.0	3.328757955523466E-5	1.1650652844332133E-4
10-11	0.0	9.986273866570399E-5	0.0	4.1609474444043325E-5	1.3315031822093865E-4
12-13	0.0	9.986273866570399E-5	0.0	6.657515911046933E-5	1.4979410799855598E-4
14-15	0.0	1.74759792664982E-4	0.0	6.657515911046933E-5	1.5811600288736464E-4
16-17	0.0	1.8308168755379065E-4	0.0	6.657515911046933E-5	1.8308168755379065E-4
18-19	0.0	1.9972547733140798E-4	0.0	1.0818463355451266E-4	2.0804737222021664E-4
20-21	0.0	2.163692671090253E-4	0.0	1.2482842333213E-4	2.24691161997834E-4
22-23	0.0	2.4133495177545132E-4	0.0	1.7475979266498196E-4	2.4133495177545132E-4
24-25	0.0	2.4965684666426E-4	0.0	2.0804737222021664E-4	2.8294442621949463E-4
26-27	0.0	2.4965684666426E-4	0.0	2.8294442621949463E-4	2.8294442621949463E-4
28-29	0.0	2.5797874155306864E-4	0.0	8.155456991032492E-4	2.8294442621949463E-4
30-31	0.0	3.162320057747293E-4	0.0	0.002238589725089531	2.8294442621949463E-4
32-33	0.0	3.162320057747293E-4	0.0	0.005251115674838268	2.9958821599711195E-4
34-35	0.0	3.411976904411553E-4	0.0	0.008762955317915525	3.079101108859206E-4
36-37	0.0	3.7448526999638997E-4	0.0	0.01422211836497401	3.162320057747293E-4
38-39	0.0	3.8280716488519863E-4	0.0	0.02325969621422022	3.3287579555234666E-4
40-41	0.0	4.327385342180506E-4	0.0	0.036433255823204336	3.3287579555234666E-4
42-43	0.0	4.4106042910685927E-4	0.0	0.044364021652238995	3.3287579555234666E-4
44-45	0.0	4.909917984397112E-4	0.0	0.054916184371248386	3.3287579555234666E-4
46-47	0.0	5.326012728837546E-4	0.0	0.06756546460223756	3.411976904411553E-4
48-49	0.0	5.409231677725632E-4	0.0	0.07912457660279279	3.8280716488519863E-4
50-51	0.0	5.825326422166066E-4	0.0	0.09186539767755886	3.8280716488519863E-4
52-53	0.0	5.825326422166066E-4	0.0	0.10445642464432638	4.160947444404333E-4
54-55	0.0	6.158202217718412E-4	0.0	0.15668463696648954	4.577042188844766E-4
56-57	0.0	6.49107801327076E-4	0.0	0.17809687251539424	4.660261137732853E-4
58-59	0.0	6.99039170659928E-4	0.0	0.2042442662560311	4.660261137732853E-4
60-61	0.0	7.240048553263539E-4	0.0	0.21854128167500436	4.8266990355090263E-4
62-63	0.0	7.656143297703973E-4	0.0	0.2270296144615892	4.8266990355090263E-4
64-65	0.0	8.488332786584839E-4	0.0	0.23530989987595385	4.8266990355090263E-4
66-67	0.0	8.488332786584839E-4	0.0	0.240868925661678	4.9931369332852E-4
68-69	0.0	8.571551735472925E-4	0.0	0.24608675375696104	5.159574831061373E-4
70-71	0.0	8.654770684361012E-4	0.0	0.25090513089758126	5.159574831061373E-4
72-73	0.0	8.654770684361012E-4	0.0	0.2549911812879863	5.326012728837546E-4
74-75	0.0	9.154084377689532E-4	0.0	0.2583948362975091	5.326012728837546E-4
76-77	0.0	9.570179122129967E-4	0.0	0.26148225930125707	5.492450626613719E-4
78-79	0.0	9.653398071018053E-4	0.0	0.2639871496627885	5.492450626613719E-4
80-81	0.0	9.653398071018053E-4	0.0	0.26497745515455673	5.658888524389893E-4
82-83	0.0	9.653398071018053E-4	0.0	0.26537690610921955	5.825326422166066E-4
84-85	0.0	9.653398071018053E-4	0.0	0.2655683096916621	5.825326422166066E-4
86-87	0.0	9.653398071018053E-4	0.0	0.2657014600098831	5.908545371054153E-4
88-89	0.0	9.653398071018053E-4	0.0	0.2657347475894383	5.991764319942239E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8405	0.0	52.288025	1
GTATCAA	15390	0.0	37.05049	1
TCAACGC	19085	0.0	29.30589	4
ATCAACG	19140	0.0	29.244137	3
CAACGCA	19575	0.0	28.572306	5
TATCAAC	19810	0.0	28.480135	2
AACGCAG	20150	0.0	27.863642	6
ACGCAGA	23380	0.0	23.977442	7
CGCAGAG	23615	0.0	23.720633	8
TGGTATC	3700	0.0	22.779648	2
GTGGTAT	4005	0.0	22.011032	1
GCAGAGT	27090	0.0	20.615734	9
TACATGG	18330	0.0	19.471899	2
GTACATG	18715	0.0	19.39285	1
GAGTACT	16370	0.0	19.172083	12-13
AGAGTAC	24735	0.0	18.989109	10-11
CAGAGTA	26535	0.0	18.559706	10-11
ACATGGG	18395	0.0	18.53289	3
GTACTTT	17590	0.0	17.830502	14-15
AGTACTT	17580	0.0	17.155518	12-13
>>END_MODULE