FastQCFastQC Report
Fri 27 May 2016
SRR2140399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7474211
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106150.051.2025451
GTATCAA204050.039.7856941
TCAACGC254750.031.2842184
ATCAACG255650.031.1740843
TATCAAC261600.030.7866122
CAACGCA259350.030.729345
AACGCAG268550.029.6766136
ACGCAGA307800.025.8783267
CGCAGAG311500.025.5709448
GCAGAGT357350.022.2301039
GTGGTAT51250.021.2607631
TGGTATC50950.020.7945212
GTACATG249250.020.1817991
TACATGG248300.020.0337982
AGAGTAC325050.019.90229810-11
GAGTACT210050.019.81153312-13
ACATGGG252600.019.0905193
CAGAGTA349150.018.92932910-11
AGTACTT225400.018.39548512-13
GTACTTT225900.018.3647914-15
Produced by FastQC (version 0.11.5)