##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7474211
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94299264497617	34.0	31.0	34.0	31.0	34.0
2	33.04973287481448	34.0	33.0	34.0	31.0	34.0
3	33.109428941730435	34.0	33.0	34.0	31.0	34.0
4	36.473819510848706	37.0	37.0	37.0	35.0	37.0
5	36.43529330386846	37.0	37.0	37.0	35.0	37.0
6	36.467139608448306	37.0	37.0	37.0	35.0	37.0
7	36.42524087157828	37.0	37.0	37.0	35.0	37.0
8	36.41063946415213	37.0	37.0	37.0	35.0	37.0
9	38.19825758197086	39.0	39.0	39.0	37.0	39.0
10-11	38.15270595116996	39.0	39.0	39.0	37.0	39.0
12-13	38.12077495537656	39.0	39.0	39.0	37.0	39.0
14-15	39.57995827251867	41.0	40.0	41.0	37.0	41.0
16-17	39.5598916728468	41.0	40.0	41.0	37.0	41.0
18-19	39.57167218051511	41.0	40.0	41.0	37.0	41.0
20-21	39.54717423411111	41.0	40.0	41.0	37.0	41.0
22-23	39.46750792329513	41.0	39.0	41.0	36.5	41.0
24-25	39.31075270793537	41.0	39.0	41.0	36.0	41.0
26-27	39.31458595228307	40.5	39.0	41.0	36.0	41.0
28-29	39.245336035437	41.0	39.0	41.0	36.0	41.0
30-31	39.156268711018846	41.0	39.0	41.0	36.0	41.0
32-33	38.955743084287505	40.0	39.0	41.0	35.0	41.0
34-35	38.87464384607438	40.0	38.0	41.0	35.0	41.0
36-37	39.061591587786445	40.5	39.0	41.0	35.0	41.0
38-39	39.13070430103104	41.0	39.0	41.0	35.0	41.0
40-41	39.10444494311481	41.0	39.0	41.0	35.0	41.0
42-43	38.95657426144417	40.5	39.0	41.0	35.0	41.0
44-45	38.97445924137423	40.0	39.0	41.0	35.0	41.0
46-47	38.92192378655045	40.0	38.5	41.0	35.0	41.0
48-49	38.8527395877567	40.0	38.0	41.0	35.0	41.0
50-51	38.777967789957145	40.0	38.0	41.0	35.0	41.0
52-53	38.66068325922981	40.0	38.0	41.0	35.0	41.0
54-55	38.51999724579227	40.0	38.0	41.0	34.5	41.0
56-57	38.37008528929941	40.0	37.5	41.0	34.0	41.0
58-59	38.2053131873146	40.0	37.0	41.0	34.0	41.0
60-61	38.01797553332882	40.0	36.5	41.0	34.0	41.0
62-63	37.78398922219263	39.0	36.0	41.0	34.0	41.0
64-65	37.50607290932052	39.0	35.5	41.0	33.0	41.0
66-67	37.180021203154055	38.5	35.0	40.0	33.0	41.0
68-69	36.843292534946414	37.5	35.0	40.0	33.0	41.0
70-71	36.482812413663574	37.0	35.0	40.0	32.0	41.0
72-73	36.142725252137396	36.5	35.0	39.0	32.0	41.0
74-75	35.80406195351633	36.0	35.0	39.0	32.0	40.5
76-77	34.95162227770672	35.0	34.0	37.0	30.5	39.0
78-79	35.180514075696934	35.0	34.0	37.0	31.0	39.0
80-81	34.9680070267152	35.0	34.0	37.0	31.0	39.0
82-83	34.70599072091257	35.0	34.0	36.5	31.0	38.5
84-85	34.45033361174873	35.0	34.0	36.0	31.0	37.0
86-87	34.239667319255865	35.0	34.0	36.0	31.0	37.0
88-89	34.04604956253259	35.0	34.0	35.0	31.0	36.5
90-91	33.85274730473472	35.0	34.0	35.0	31.0	36.0
92-93	33.72505862489031	35.0	34.0	35.0	31.0	36.0
94-95	33.654182905253265	35.0	34.0	35.0	30.5	36.0
96-97	33.62887385814457	35.0	34.0	35.0	31.0	35.5
98-99	33.656300671572254	35.0	34.0	35.0	31.0	35.0
100-101	32.23423692680806	33.5	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	0.0
18	2.0
19	8.0
20	26.0
21	143.0
22	571.0
23	2131.0
24	5352.0
25	11389.0
26	20427.0
27	32222.0
28	47391.0
29	63973.0
30	83999.0
31	106819.0
32	138240.0
33	183477.0
34	267929.0
35	406676.0
36	742575.0
37	1667236.0
38	2410944.0
39	1211093.0
40	71585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.198689787775535	21.04994655621752	15.644518369769964	24.10684528623698
2	13.515086221318112	21.377158878754827	41.70688097643887	23.4008739234882
3	16.975027056374795	26.437593596860886	30.90005912331325	25.68732022345107
4	11.926425926810449	18.492806395967786	38.954530171687736	30.626237505534032
5	13.134687563593687	37.85337016933832	35.4076799297424	13.604262337325595
6	29.77326430127924	38.11580596689228	18.66387466553317	13.44705506629531
7	26.41030402702415	32.92311796680958	22.847249018089418	17.819328988076858
8	24.59446683934129	36.7030706942427	20.806328673419788	17.896133792996228
9	25.515598297659636	17.1584284619278	21.3203158981566	36.00565734225596
10-11	24.168952415178	27.106687247657312	28.455973480010133	20.268386857154557
12-13	24.99734754611557	24.5793168001278	28.863936273674906	21.55939938008172
14-15	22.126059941620717	25.869623258751556	26.815535421164586	25.188781378463137
16-17	21.490881908943653	29.04753641423097	27.401080810312052	22.06050086651333
18-19	21.491247904460806	28.053386244164933	29.46859266731869	20.986773184055572
20-21	22.41241280549894	27.12654034336392	29.423449096728156	21.03759775440899
22-23	22.08327925271061	27.013324573387514	29.296218192230857	21.607177981671015
24-25	21.785873469731612	27.191567691138136	29.34734132292916	21.67521751620109
26-27	21.746941366837028	27.348515621380965	29.26106004054525	21.643482971236757
28-29	21.655005871592266	27.430370384499962	29.314444922960035	21.600178820947736
30-31	21.97140482186134	27.273310342379474	29.193184519615638	21.562100316143542
32-33	21.814669655722334	27.419344407283358	29.09979220511842	21.666193731875886
34-35	21.862931796915884	27.478083083889505	29.12636229256759	21.532622826627026
36-37	21.880944087279712	27.359004102227647	29.11546773173528	21.644584078757358
38-39	21.72542628258738	27.546163898668556	29.086173064135153	21.64223675460891
40-41	22.028643012658506	27.476443091432262	28.908949300335884	21.585964595573355
42-43	21.927530026154948	27.54407448519684	28.69147409521629	21.836921393431922
44-45	22.0108452357239	27.661097835464183	28.4805079340402	21.847548994771714
46-47	22.061153335365706	27.65976711834355	28.1867117993139	22.092367746976848
48-49	22.01974142391926	27.79817724130101	28.115870776333068	22.06621055844666
50-51	22.016068912333516	27.94166399674074	27.93077982854083	22.111487262384912
52-53	22.209508669438456	27.85924006480392	27.781514733724105	22.149736532033522
54-55	22.191506143111358	27.748185879037774	27.854533020697087	22.205774957153782
56-57	22.178558310279474	27.888655955860166	27.8045188068424	22.128266927017965
58-59	22.11963860242963	27.9128078799649	27.857680432619762	22.1098730849857
60-61	22.22079073116033	27.87775891843804	27.848706474336428	22.052743876065204
62-63	22.15907963751703	27.956525014114042	27.76187425561001	22.122521092758916
64-65	22.14385565036813	27.87614563945574	27.766405379617865	22.213593330558258
66-67	22.211598302730305	27.958029351888097	27.609593096049455	22.220779249332146
68-69	22.18824941836586	27.90889933699866	27.67858652328476	22.224264721350718
70-71	22.154002226590304	27.69402758069074	27.71483431049045	22.437135882228503
72-73	22.20560741547865	27.70475442540939	27.74386132661671	22.345776832495247
74-75	22.054684647829596	27.852874820752294	27.67880854950145	22.413631981916662
76-77	22.17964965939703	27.82487458408483	27.621906156960087	22.37356959955806
78-79	22.21047207266395	27.765915569572492	27.572910558469882	22.45070179929368
80-81	22.176552196958184	27.772020515938333	27.584437444821376	22.466989842282107
82-83	22.217348972055937	27.69625156947922	27.701274556382455	22.385124902082385
84-85	22.335853620893417	27.718898477689564	27.635898008244446	22.309349893172573
86-87	22.161207516069393	27.76953727787116	27.68908821674637	22.38016698931308
88-89	22.197041765560787	27.744957769491435	27.67442327004615	22.383577194901623
90-91	22.25740584362768	27.667572558857817	27.75004510684219	22.324976490672316
92-93	22.253321593354375	27.59929137957614	27.789414329268347	22.35797269780114
94-95	22.09798615051751	27.691057863792885	27.790383924448303	22.4205720612413
96-97	22.102949112216404	27.705733763847455	27.874060333642493	22.317256790293648
98-99	22.286376038818258	28.26282581549909	27.83504686486656	21.615751280816088
100-101	20.17096983862419	32.002393177227006	25.96816927863708	21.858467705511725
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1452.0
1	1321.0
2	1914.5
3	2821.5
4	3986.5
5	6091.0
6	7692.0
7	9382.5
8	10883.0
9	11076.5
10	10282.0
11	9568.0
12	10709.5
13	14837.0
14	24976.5
15	38209.5
16	48205.0
17	52923.0
18	51949.0
19	47391.0
20	41859.0
21	36724.0
22	33885.5
23	35205.0
24	40617.0
25	49998.5
26	63281.0
27	78000.0
28	92853.5
29	108417.0
30	123648.0
31	139142.0
32	156449.5
33	175205.5
34	193679.0
35	210182.5
36	228198.5
37	243476.0
38	253416.5
39	262530.5
40	273495.5
41	282938.5
42	287980.0
43	292894.5
44	297709.5
45	300735.0
46	301340.0
47	302349.5
48	302583.5
49	298952.5
50	292489.0
51	283499.0
52	270732.0
53	256631.5
54	242836.5
55	227009.5
56	209036.5
57	188948.0
58	168174.5
59	146998.5
60	123920.0
61	100425.5
62	78036.5
63	59404.5
64	44573.0
65	32905.0
66	24452.5
67	18256.5
68	13542.0
69	9593.5
70	6603.0
71	4571.0
72	3131.0
73	2027.0
74	1305.0
75	857.5
76	549.0
77	336.5
78	229.0
79	166.0
80	125.0
81	83.5
82	53.5
83	35.5
84	22.0
85	17.0
86	13.5
87	11.5
88	11.0
89	9.5
90	6.5
91	4.5
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.6758677270416904E-5
2	0.028912750790685466
3	2.6758677270416904E-5
4	2.6758677270416904E-5
5	2.6758677270416904E-5
6	2.6758677270416904E-5
7	9.365537044645916E-5
8	5.084148681379211E-4
9	4.0138015905625353E-4
10-11	0.0
12-13	0.0
14-15	0.0020804871577749144
16-17	5.351735454083381E-5
18-19	1.3379338635208452E-5
20-21	1.806252765406433E-4
22-23	0.0018665829402748986
24-25	7.628156542887336E-4
26-27	0.014537623261910677
28-29	0.3104452420190665
30-31	0.31166645074824945
32-33	0.3294332835657035
34-35	0.2142358150575326
36-37	0.07381938325872092
38-39	0.0658962329342822
40-41	0.027508541046516777
42-43	0.01158285214829905
44-45	0.015148830844046638
46-47	0.011429027108096663
48-49	0.008261754450047615
50-51	0.008593902722298768
52-53	0.0040373299283747496
54-55	0.002169564795426673
56-57	0.004357133702582199
58-59	0.00528631644330756
60-61	0.004536001123566738
62-63	0.0030054367082249706
64-65	0.0011124231356953282
66-67	0.00792182425436682
68-69	0.011649302602223204
70-71	0.01205795662254319
72-73	0.010336552542402455
74-75	0.011057216024051448
76-77	0.010244914514038585
78-79	0.0028734213091163416
80-81	0.0024172908572481417
82-83	0.001755972716594334
84-85	1.649780476085401E-5
86-87	0.002883813968994832
88-89	0.0
90-91	0.008636552861592072
92-93	0.019028570706968485
94-95	0.0015620924737682548
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	100.0
20-21	390.0
22-23	827.0
24-25	2029.0
26-27	2619.0
28-29	4891.0
30-31	13174.0
32-33	17759.0
34-35	26087.0
36-37	36658.0
38-39	44292.0
40-41	47195.0
42-43	56324.0
44-45	68942.0
46-47	68599.0
48-49	60056.0
50-51	52272.0
52-53	45729.0
54-55	50719.0
56-57	52404.0
58-59	52494.0
60-61	53369.0
62-63	51585.0
64-65	55341.0
66-67	58715.0
68-69	59688.0
70-71	58970.0
72-73	56542.0
74-75	56734.0
76-77	57662.0
78-79	61684.0
80-81	63091.0
82-83	60764.0
84-85	61659.0
86-87	65618.0
88-89	71567.0
90-91	77320.0
92-93	80467.0
94-95	91493.0
96-97	145675.0
98-99	663984.0
100-101	4818723.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.32389756294194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.68018002728006	76.51862494073835
2	7.2927264395921	12.44487687372629
3	1.7362151315118663	4.4442192608504465
4	0.6259343718387925	2.1362864089559017
5	0.26746275430886596	1.1410482325275992
6	0.14151893299689308	0.7244968165346248
7	0.07694525339744457	0.45956882431867185
8	0.04817178142093691	0.328816331470756
9	0.030765094491743115	0.2362497993834947
>10	0.09697373605169565	1.2592308939968557
>50	0.002468145420188141	0.1435194929755828
>100	5.395103357898465E-4	0.08869360226950619
>500	7.411614632082063E-5	0.043613610863991584
>1k	2.4705207308450057E-5	0.030754911388022003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3379338635208452E-5
2	0.0	0.0	0.0	0.0	1.3379338635208452E-5
3	0.0	0.0	0.0	0.0	1.3379338635208452E-5
4	0.0	2.6758677270416904E-5	0.0	0.0	1.3379338635208452E-5
5	0.0	2.6758677270416904E-5	0.0	0.0	1.3379338635208452E-5
6	0.0	2.6758677270416904E-5	0.0	0.0	4.0138015905625356E-5
7	0.0	2.6758677270416904E-5	0.0	0.0	4.0138015905625356E-5
8	0.0	4.0138015905625356E-5	0.0	0.0	4.0138015905625356E-5
9	0.0	1.4717272498729298E-4	0.0	0.0	4.0138015905625356E-5
10-11	0.0	1.4717272498729298E-4	0.0	0.0	5.351735454083381E-5
12-13	0.0	1.4717272498729298E-4	0.0	0.0	6.689669317604226E-5
14-15	0.0	1.739314022577099E-4	0.0	1.3379338635208452E-5	1.2710371703448029E-4
16-17	0.0	1.739314022577099E-4	0.0	2.6758677270416904E-5	1.4717272498729298E-4
18-19	0.0	1.9400041021052256E-4	0.0	5.351735454083381E-5	1.4717272498729298E-4
20-21	0.0	2.4082809543375213E-4	0.0	1.0703470908166761E-4	1.5386239430489722E-4
22-23	0.0	2.809661113393775E-4	0.0	2.6758677270416904E-4	1.6055206362250142E-4
24-25	0.0	2.9434544997458597E-4	0.0	9.633123817350086E-4	1.6055206362250142E-4
26-27	6.689669317604226E-6	2.9434544997458597E-4	0.0	0.0032980069735788833	1.6055206362250142E-4
28-29	1.3379338635208452E-5	2.9434544997458597E-4	0.0	0.018510315001810895	1.6055206362250142E-4
30-31	1.3379338635208452E-5	3.2110412724500284E-4	0.0	0.026156607031832525	1.6055206362250142E-4
32-33	1.3379338635208452E-5	3.2110412724500284E-4	0.0	0.028752198727062965	1.8731074089291833E-4
34-35	1.3379338635208452E-5	3.679318124682324E-4	0.0	0.032023447023371426	2.341384261161479E-4
36-37	1.3379338635208452E-5	4.14759497691462E-4	0.0	0.03718118206724429	2.4751776475135637E-4
38-39	1.3379338635208452E-5	4.415181749618789E-4	0.0	0.045355957973356655	2.5420743406896057E-4
40-41	1.3379338635208452E-5	5.485528840435465E-4	0.0	0.05781212224273572	2.5420743406896057E-4
42-43	1.3379338635208452E-5	5.753115613139634E-4	0.0	0.06587986343976641	2.5420743406896057E-4
44-45	1.3379338635208452E-5	6.555875931252141E-4	0.0	0.07638933393772265	2.5420743406896057E-4
46-47	1.3379338635208452E-5	7.09104947666048E-4	0.0	0.08883211886846651	2.5420743406896057E-4
48-49	2.0069007952812678E-5	7.157946169836522E-4	0.0	0.09966269349366777	2.6758677270416904E-4
50-51	2.6758677270416904E-5	8.094499874301114E-4	0.0	0.1117709949585314	2.809661113393775E-4
52-53	3.344834658802113E-5	8.161396567477156E-4	0.0	0.12433419393699216	2.876557806569817E-4
54-55	4.682768522322958E-5	8.161396567477156E-4	0.0	0.17527602579054832	2.9434544997458597E-4
56-57	5.351735454083381E-5	8.428983340181325E-4	0.0	0.1981747638647076	2.9434544997458597E-4
58-59	5.351735454083381E-5	8.495880033357367E-4	0.0	0.22484647543399564	2.9434544997458597E-4
60-61	5.351735454083381E-5	8.696570112885494E-4	0.0	0.2380652620055816	2.9434544997458597E-4
62-63	5.351735454083381E-5	8.696570112885494E-4	0.0	0.24607948584807146	3.077247886097944E-4
64-65	5.351735454083381E-5	9.231743658293832E-4	0.0	0.2541806753916902	3.1441445792739864E-4
66-67	5.351735454083381E-5	9.365537044645916E-4	0.0	0.25983344596506575	3.2110412724500284E-4
68-69	5.351735454083381E-5	9.365537044645916E-4	0.0	0.26493097398508014	3.2779379656260705E-4
70-71	5.351735454083381E-5	9.365537044645916E-4	0.0	0.2700017433278241	3.478628045154198E-4
72-73	5.351735454083381E-5	9.432433737821958E-4	0.0	0.2745373391251599	4.013801590562536E-4
74-75	5.351735454083381E-5	9.700020510526128E-4	0.0	0.2777149320510218	4.013801590562536E-4
76-77	5.351735454083381E-5	0.001010140066958238	0.0	0.28037742043942837	4.013801590562536E-4
78-79	5.351735454083381E-5	0.0010368987442286551	0.0	0.2830131501505644	4.013801590562536E-4
80-81	6.689669317604226E-5	0.0010435884135462593	0.0	0.2840366695561578	4.080698283738578E-4
82-83	6.689669317604226E-5	0.001063657421499072	0.0	0.2844915670697549	4.3482850564427467E-4
84-85	6.689669317604226E-5	0.0010703470908166761	0.0	0.2847792228504119	4.548975135970874E-4
86-87	6.689669317604226E-5	0.0010837264294518846	0.0	0.28485949888222317	4.548975135970874E-4
88-89	6.689669317604226E-5	0.0011372437839927184	0.0	0.2848996368981288	4.548975135970874E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10615	0.0	51.202545	1
GTATCAA	20405	0.0	39.785694	1
TCAACGC	25475	0.0	31.284218	4
ATCAACG	25565	0.0	31.174084	3
TATCAAC	26160	0.0	30.786612	2
CAACGCA	25935	0.0	30.72934	5
AACGCAG	26855	0.0	29.676613	6
ACGCAGA	30780	0.0	25.878326	7
CGCAGAG	31150	0.0	25.570944	8
GCAGAGT	35735	0.0	22.230103	9
GTGGTAT	5125	0.0	21.260763	1
TGGTATC	5095	0.0	20.794521	2
GTACATG	24925	0.0	20.181799	1
TACATGG	24830	0.0	20.033798	2
AGAGTAC	32505	0.0	19.902298	10-11
GAGTACT	21005	0.0	19.811533	12-13
ACATGGG	25260	0.0	19.090519	3
CAGAGTA	34915	0.0	18.929329	10-11
AGTACTT	22540	0.0	18.395485	12-13
GTACTTT	22590	0.0	18.36479	14-15
>>END_MODULE