FastQCFastQC Report
Fri 27 May 2016
SRR2140398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11959762
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144100.046.5183871
GTATCAA260450.035.868761
ATCAACG340900.028.4113863
TCAACGC345400.028.0655824
CAACGCA353300.027.4379045
TATCAAC352700.027.0417882
AACGCAG369700.026.3133666
ACGCAGA440700.021.985367
TGGTATC76600.021.9059872
GTGGTAT79400.021.845231
CGCAGAG445700.021.7683328
GCAGAGT522050.018.5763829
AGAGTAC483250.018.23371310-11
AGTACTT344200.016.82036412-13
GAGTACT309100.016.73437312-13
CAGAGTA522900.015.9703269
TACATGG363350.015.2541932
GTACATG368700.015.068221
GTACTTT344000.014.92950314-15
ACATGGG370050.014.6960683
Produced by FastQC (version 0.11.5)