##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140398_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11959762
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.789992560052617	27.0	18.0	32.0	18.0	33.0
2	26.71478320387981	28.0	18.0	31.0	18.0	33.0
3	28.404222007093452	30.0	27.0	32.0	18.0	33.0
4	29.188741046853607	31.0	28.0	33.0	15.0	33.0
5	30.7822124721211	33.0	32.0	33.0	27.0	33.0
6	33.72379943681153	36.0	33.0	38.0	26.0	38.0
7	35.31057424052418	38.0	35.0	38.0	29.0	38.0
8	35.86470148820687	38.0	36.0	38.0	31.0	38.0
9	36.064353203684156	38.0	37.0	38.0	32.0	38.0
10-11	36.1793598819107	38.0	37.0	38.0	33.0	38.0
12-13	36.17587482092035	38.0	37.0	38.0	33.0	38.0
14-15	36.295105663473905	38.0	37.0	38.0	33.0	38.0
16-17	36.317267475724016	38.0	37.0	38.0	33.0	38.0
18-19	36.35281350080378	38.0	37.0	38.0	33.0	38.0
20-21	36.37136459472647	38.0	37.0	38.0	33.0	38.0
22-23	36.37018330639724	38.0	37.0	38.0	33.0	38.0
24-25	36.36706329119005	38.0	37.0	38.0	33.0	38.0
26-27	36.30576504684858	38.0	37.0	38.0	33.0	38.0
28-29	36.277427196196065	38.0	37.0	38.0	33.0	38.0
30-31	36.27606090054377	38.0	37.0	38.0	33.0	38.0
32-33	36.25134817587056	38.0	37.0	38.0	33.0	38.0
34-35	36.22130027294466	38.0	37.0	38.0	33.0	38.0
36-37	36.19552474386933	38.0	37.0	38.0	33.0	38.0
38-39	36.18213931971868	38.0	37.0	38.0	33.0	38.0
40-41	36.17347442389797	38.0	37.0	38.0	33.0	38.0
42-43	36.13386914236369	38.0	37.0	38.0	33.0	38.0
44-45	36.08203075887435	38.0	37.0	38.0	33.0	38.0
46-47	36.040896754422704	38.0	37.0	38.0	31.5	38.0
48-49	35.989023933240034	38.0	37.0	38.0	31.0	38.0
50-51	35.956184427055234	38.0	37.0	38.0	31.0	38.0
52-53	35.935111431744495	38.0	37.0	38.0	31.0	38.0
54-55	35.89992049471415	38.0	37.0	38.0	31.0	38.0
56-57	35.86826427066935	38.0	37.0	38.0	31.0	38.0
58-59	35.83946164102842	38.0	37.0	38.0	31.0	38.0
60-61	35.838490778946095	38.0	37.0	38.0	31.0	38.0
62-63	35.83378989021019	38.0	37.0	38.0	31.0	38.0
64-65	35.80442433138729	38.0	37.0	38.0	31.0	38.0
66-67	35.78429064351841	38.0	37.0	38.0	31.0	38.0
68-69	35.77546452994079	38.0	37.0	38.0	31.0	38.0
70-71	35.745310054139075	38.0	37.0	38.0	30.0	38.0
72-73	35.7366484122934	38.0	37.0	38.0	30.5	38.0
74-75	35.75314926370193	38.0	37.0	38.0	31.0	38.0
76-77	35.71708299418887	38.0	37.0	38.0	30.0	38.0
78-79	35.71391737466398	38.0	37.0	38.0	29.0	38.0
80-81	35.68821973700789	38.0	36.5	38.0	29.0	38.0
82-83	35.668662212227794	38.0	36.0	38.0	29.0	38.0
84-85	35.64523419338684	38.0	36.0	38.0	29.0	38.0
86-87	35.61774759262002	38.0	36.0	38.0	29.0	38.0
88-89	35.538946366057665	38.0	36.0	38.0	29.0	38.0
90-91	35.55765399848459	38.0	36.0	38.0	29.0	38.0
92-93	35.591360647665766	38.0	36.0	38.0	29.0	38.0
94-95	35.567082086442554	38.0	36.0	38.0	29.0	38.0
96-97	35.56638217745568	38.0	36.0	38.0	29.0	38.0
98-99	35.58935945087755	38.0	36.0	38.0	29.0	38.0
100-101	34.99496740644841	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	37.0
19	98.0
20	232.0
21	1116.0
22	4445.0
23	13071.0
24	29337.0
25	54635.0
26	87485.0
27	130256.0
28	180641.0
29	237494.0
30	303164.0
31	379128.0
32	475025.0
33	611074.0
34	831452.0
35	1270128.0
36	2645924.0
37	4705017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.437614728453624	25.83885030488065	13.982485604646646	23.741049362019076
2	13.187862768506598	26.168129432676	38.71294428768733	21.931063511130073
3	19.060651198041647	28.124541679815284	29.89680782552084	22.91799929662223
4	13.634831593611562	19.729581189984238	38.97618086651381	27.659406349890393
5	13.49202186242181	39.968790675919536	34.143666458944715	12.395521002713938
6	28.46334233072531	40.19445370233956	18.786310296141345	12.555893670793782
7	24.96723597008034	34.0773252845667	23.75121678842773	17.20422195692523
8	23.557191188252744	37.926156055613816	21.371269762726048	17.14538299340739
9	24.614558767397437	17.580514952881483	21.95607176914873	35.848854510572345
10-11	23.23399077673954	28.028981680404673	29.41002504899345	19.327002493862334
12-13	24.14742133444718	25.35923897813514	29.75102806188903	20.742311625528654
14-15	21.288359806608902	26.88575238389082	27.47273604840923	24.353151761091045
16-17	20.642943638280293	30.027602984425062	28.175450045705357	21.15400333158929
18-19	20.657342512334274	29.094182643433875	30.119533315127846	20.128941529104008
20-21	21.504266460560007	28.179635550923997	29.98643296521465	20.329665023301345
22-23	21.15129203191725	28.094192401762086	29.914935437914792	20.83958012840587
24-25	20.988583791342048	28.21901374527914	29.940648426602085	20.851754036776736
26-27	20.924402179295356	28.3714069864858	29.850225303594417	20.85396553062443
28-29	20.87897177899819	28.41085077370449	29.89803792477434	20.812139522522983
30-31	21.133050828507084	28.248331608192935	29.816108604197407	20.80250895910257
32-33	20.99278570924585	28.393715582487133	29.755360631059364	20.858138077207652
34-35	21.09483702030297	28.40995739558445	29.771207199112677	20.72399838499991
36-37	21.070550652462913	28.27623538293328	29.794214974683044	20.858998989920767
38-39	20.962282859849424	28.363522804078485	29.826543172652187	20.847651163419904
40-41	21.150730748755507	28.281399938556174	29.725628365472662	20.84224094721566
42-43	21.07770225882405	28.36961204312669	29.512143574442973	21.040542123606283
44-45	21.108189962240953	28.488413883262286	29.331141478157964	21.072254676338797
46-47	21.19608001936739	28.433592668258555	29.142117803194573	21.22820950917948
48-49	21.13481856001657	28.51098561499763	29.194000613901693	21.16019521108411
50-51	21.09345918397745	28.624961845158836	29.055823151416178	21.225755819447535
52-53	21.244740307390543	28.536570195924966	28.95483638128343	21.26385311540105
54-55	21.22853977625942	28.51023081921973	28.977192898513316	21.284036506007535
56-57	21.255767453742102	28.518188457478416	28.979620799912777	21.246423288866705
58-59	21.201978879062192	28.574148905460188	29.04027794737565	21.18359426810197
60-61	21.28825228476519	28.497427157014215	29.051317105308737	21.16300345291186
62-63	21.215960966730506	28.60010373783076	28.968743200434545	21.215192095004184
64-65	21.147393793970906	28.593718602148883	28.967769928978583	21.291117674901628
66-67	21.242156130748473	28.666583613520793	28.80277487294412	21.288485382786618
68-69	21.20304742279692	28.614828958978332	28.852937459987636	21.329186158237114
70-71	21.183833386553268	28.442814904563413	28.9023686280631	21.47098308082022
72-73	21.245142059824243	28.440145606663066	28.87331794001445	21.441394393498243
74-75	21.13826719891299	28.540275982061864	28.856765263413127	21.46469155561202
76-77	21.32162193238311	28.496844644805545	28.74785068628084	21.433682736530503
78-79	21.32852522533507	28.561331012143743	28.629612798538062	21.48053096398312
80-81	21.29302727089211	28.57197915910409	28.660260273179976	21.47473329682382
82-83	21.29828754085516	28.537763770055424	28.72846727589195	21.435481413197465
84-85	21.468520121214596	28.54658956260004	28.667060858821024	21.31782945736434
86-87	21.31599758523146	28.61836531449028	28.658308969891017	21.40732813038725
88-89	21.396675877204252	28.67215969978178	28.592487789959964	21.338676633054007
90-91	21.41212779258175	28.647219896581838	28.67916526643826	21.261487044398148
92-93	21.444943055754244	28.633912763965203	28.61399820694837	21.307145973332183
94-95	21.322098256839276	28.677494530219327	28.660520180241022	21.33988703270038
96-97	21.349436515327163	28.67348296242802	28.707495662892	21.269584859352815
98-99	21.465099491789456	29.243839316989405	28.778108511927158	20.512952679293978
100-101	22.04687764004779	31.76095570697064	26.329261331943027	19.862905321038546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3166.0
1	2929.5
2	3153.0
3	4008.5
4	4953.5
5	6571.0
6	8746.5
7	13030.0
8	16839.5
9	17689.0
10	17179.5
11	16378.5
12	17571.5
13	22289.0
14	32971.5
15	46099.0
16	54629.5
17	56745.5
18	54435.5
19	50559.5
20	47361.5
21	46047.0
22	48784.5
23	57395.5
24	72261.5
25	94633.5
26	123324.5
27	154282.0
28	188007.5
29	223737.5
30	256245.5
31	287859.5
32	320331.0
33	352698.5
34	380969.0
35	405010.5
36	430780.5
37	453440.0
38	468059.5
39	475780.5
40	486331.0
41	495714.5
42	496298.5
43	495936.0
44	496672.5
45	493850.0
46	485710.5
47	475220.0
48	460269.5
49	441812.5
50	420873.0
51	398281.5
52	372427.5
53	344229.0
54	317620.0
55	288924.5
56	259794.0
57	229480.5
58	199370.5
59	169773.5
60	138735.0
61	109791.5
62	83584.5
63	62132.0
64	45750.0
65	32805.5
66	23110.5
67	16946.5
68	12510.5
69	8936.5
70	6369.5
71	4370.5
72	2909.0
73	1908.5
74	1279.5
75	825.0
76	498.0
77	299.5
78	192.5
79	126.5
80	84.5
81	47.5
82	30.5
83	24.0
84	20.0
85	15.5
86	12.5
87	11.0
88	9.0
89	9.5
90	8.0
91	4.0
92	1.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.002742529491807613
4	7.608847065685755E-4
5	0.05253449023483912
6	0.0
7	0.0
8	0.0
9	2.257570008500169E-4
10-11	0.0
12-13	4.180685200926239E-6
14-15	4.975015389102225E-4
16-17	3.762616680833615E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.25465907190861E-4
28-29	0.0
30-31	2.5125430335808076E-5
32-33	0.0
34-35	2.9433965088542205E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.2010649156284069E-4
44-45	2.4622024919649044E-4
46-47	1.3062521144956106E-4
48-49	0.0020185066341950383
50-51	6.895006291693242E-4
52-53	0.00546304609663132
54-55	0.0011816246094092859
56-57	0.002802269856604878
58-59	0.008218647221552957
60-61	0.009180537206286213
62-63	0.007619076381033555
64-65	0.010732680287925253
66-67	0.004942727258486165
68-69	0.004570274407010433
70-71	0.004815735768763215
72-73	0.007416702154026326
74-75	0.005446564817679753
76-77	0.0027303756933456935
78-79	1.8242627537906947E-4
80-81	3.1370594728216374E-4
82-83	0.0
84-85	0.0
86-87	9.792277594561358E-4
88-89	3.625922674298233E-5
90-91	1.1521267657848307E-4
92-93	2.1185694465740483E-5
94-95	7.769133916775645E-4
96-97	2.877822376784813E-4
98-99	1.008486639176815E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	157.0
20-21	574.0
22-23	1140.0
24-25	1749.0
26-27	3693.0
28-29	7697.0
30-31	19657.0
32-33	26161.0
34-35	35865.0
36-37	55810.0
38-39	64526.0
40-41	68273.0
42-43	77271.0
44-45	90637.0
46-47	90533.0
48-49	84175.0
50-51	76130.0
52-53	67163.0
54-55	71712.0
56-57	72703.0
58-59	81968.0
60-61	81712.0
62-63	79342.0
64-65	86192.0
66-67	90963.0
68-69	92787.0
70-71	93476.0
72-73	88020.0
74-75	89872.0
76-77	94311.0
78-79	98918.0
80-81	100209.0
82-83	95007.0
84-85	94638.0
86-87	98202.0
88-89	104253.0
90-91	107443.0
92-93	107439.0
94-95	115914.0
96-97	185394.0
98-99	997978.0
100-101	8060098.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.70652398181424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.48971238465874	71.4848028311915
2	8.316221220719061	13.589790572175092
3	2.2397343003027896	5.490027129617455
4	0.8991120585724454	2.938532839043515
5	0.4177608678774487	1.7066894184946149
6	0.21454775610901927	1.051797082785961
7	0.12937218657904656	0.7399386165710431
8	0.08092855500458396	0.5289912736236491
9	0.05227274709960262	0.38439220180400935
>10	0.1577814834477443	1.8742988742106332
>50	0.0020993421137243617	0.11542829205377281
>100	4.390351825978408E-4	0.07432484363872792
>500	0.0	0.0
>1k	1.8062333232541832E-5	0.02098602479000588
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5084111205557437E-5	0.0	0.0	0.0
3	0.0	3.344548160740991E-5	0.0	0.0	0.0
4	0.0	1.4214329683149212E-4	0.0	8.361370401852478E-6	0.0
5	0.0	1.4214329683149212E-4	0.0	8.361370401852478E-6	0.0
6	0.0	1.4214329683149212E-4	0.0	8.361370401852478E-6	0.0
7	0.0	1.4214329683149212E-4	0.0	8.361370401852478E-6	0.0
8	0.0	1.588660376351971E-4	0.0	8.361370401852478E-6	0.0
9	0.0	2.1739563044816443E-4	0.0	1.6722740803704957E-5	0.0
10-11	0.0	2.2157631564909065E-4	0.0	2.0903426004631195E-5	0.0
12-13	0.0	2.257570008500169E-4	0.0	2.5084111205557437E-5	0.0
14-15	0.0	2.9682864926576296E-4	0.0	2.9264796406483675E-5	0.0
16-17	0.0	3.093707048685417E-4	0.0	3.344548160740991E-5	0.0
18-19	0.0	3.386355012750254E-4	0.0	4.598753721018863E-5	0.0
20-21	0.0	3.84623038485214E-4	0.0	6.271027801389359E-5	0.0
22-23	0.0	4.2224920529355015E-4	0.0	7.525233361667231E-5	0.0
24-25	8.361370401852478E-6	4.473333164991076E-4	0.0	1.0033644482222975E-4	0.0
26-27	8.361370401852478E-6	4.515140017000338E-4	0.0	2.0903426004631196E-4	0.0
28-29	8.361370401852478E-6	4.515140017000338E-4	0.0	5.142242797139273E-4	0.0
30-31	8.361370401852478E-6	4.8495948330744374E-4	0.0	0.0016722740803704957	0.0
32-33	1.2542055602778718E-5	4.8495948330744374E-4	0.0	0.004343731923762363	0.0
34-35	2.5084111205557437E-5	5.43489076120411E-4	0.0	0.0077593517329191	0.0
36-37	3.344548160740991E-5	6.020186689333785E-4	0.0	0.013323843735351924	0.0
38-39	3.344548160740991E-5	6.103800393352309E-4	0.0	0.024507176647829615	0.0
40-41	3.7626166808336155E-5	6.64728946947272E-4	0.0	0.058621567887387725	0.0
42-43	4.180685200926239E-5	6.856323729519032E-4	0.0	0.06909418431570796	0.0
44-45	4.180685200926239E-5	8.110529289796904E-4	0.0	0.08497242670882582	0.0
46-47	4.180685200926239E-5	9.155700590028463E-4	0.0	0.10294519238760771	0.0
48-49	4.598753721018863E-5	9.239314294046989E-4	0.0	0.11998148458138214	0.0
50-51	5.016822241111487E-5	0.0010242678742269286	0.0	0.13602695438253704	0.0
52-53	5.016822241111487E-5	0.001028448559427855	0.0	0.16504509036216608	0.0
54-55	5.852959281296735E-5	0.0010368099298297072	0.0	0.23619199111152883	0.0
56-57	5.852959281296735E-5	0.0010535326706334123	0.0	0.31433317820204115	0.0
58-59	5.852959281296735E-5	0.0010577133558343386	0.0	0.4020690378286792	0.0
60-61	5.852959281296735E-5	0.0010827974670398958	0.0	0.4255394045466791	0.0
62-63	5.852959281296735E-5	0.001120423633848232	0.0	0.4397202887482209	0.0
64-65	5.852959281296735E-5	0.0012123987082686093	0.0	0.4492313475803281	0.0
66-67	5.852959281296735E-5	0.0012123987082686093	0.0	0.45446138476668685	0.0
68-69	5.852959281296735E-5	0.0012458441898760193	0.0	0.45941967741498535	0.0
70-71	5.852959281296735E-5	0.0012667476158806507	0.0	0.46447830650810606	0.0
72-73	5.852959281296735E-5	0.0012792896714834293	0.0	0.4685921007458175	0.0
74-75	5.852959281296735E-5	0.0013336385790954702	0.0	0.47156038723847515	0.0
76-77	5.852959281296735E-5	0.001417252283113995	0.0	0.47451195099032906	0.0
78-79	5.852959281296735E-5	0.0014381557091186262	0.0	0.47714996335211357	0.0
80-81	5.852959281296735E-5	0.0014381557091186262	0.0	0.4784919633016108	0.0
82-83	5.852959281296735E-5	0.0014423363943195525	0.0	0.47897274209971735	0.0
84-85	5.852959281296735E-5	0.00146742050552511	0.0	0.4794618822682257	0.0
86-87	5.852959281296735E-5	0.0014799625611278888	0.0	0.47964165173186557	0.0
88-89	5.852959281296735E-5	0.0015259500983380774	0.0	0.47973362680628595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14410	0.0	46.518387	1
GTATCAA	26045	0.0	35.86876	1
ATCAACG	34090	0.0	28.411386	3
TCAACGC	34540	0.0	28.065582	4
CAACGCA	35330	0.0	27.437904	5
TATCAAC	35270	0.0	27.041788	2
AACGCAG	36970	0.0	26.313366	6
ACGCAGA	44070	0.0	21.98536	7
TGGTATC	7660	0.0	21.905987	2
GTGGTAT	7940	0.0	21.84523	1
CGCAGAG	44570	0.0	21.768332	8
GCAGAGT	52205	0.0	18.576382	9
AGAGTAC	48325	0.0	18.233713	10-11
AGTACTT	34420	0.0	16.820364	12-13
GAGTACT	30910	0.0	16.734373	12-13
CAGAGTA	52290	0.0	15.970326	9
TACATGG	36335	0.0	15.254193	2
GTACATG	36870	0.0	15.06822	1
GTACTTT	34400	0.0	14.929503	14-15
ACATGGG	37005	0.0	14.696068	3
>>END_MODULE