FastQCFastQC Report
Fri 27 May 2016
SRR2140397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18386058
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212300.048.207811
GTATCAA374250.033.093611
ATCAACG464550.029.4833983
TCAACGC469300.029.1471464
CAACGCA478750.028.5536585
TATCAAC479900.028.2337532
AACGCAG500350.027.3252166
ACGCAGA596100.022.8925027
CGCAGAG604850.022.5686048
GCAGAGT716450.019.0712499
GAGTACT407900.018.83235412-13
ACCGAAC19050.018.6180528
TGGTATC104950.017.813022
GTGGTAT103550.017.8031061
CAGAGTA700350.017.76188710-11
GTACTTT458850.016.7886914-15
AGAGTAC650950.016.49849910-11
CCGAACC21450.015.5266879
GGACCGA25850.015.0589596
TACATGG475150.014.4082772
Produced by FastQC (version 0.11.5)