##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140397_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18386058
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.263905998773637	18.0	18.0	32.0	18.0	33.0
2	27.445422123654783	29.0	25.0	32.0	18.0	33.0
3	29.43335243476334	31.0	28.0	33.0	25.0	33.0
4	30.619142776553844	32.0	31.0	33.0	27.0	33.0
5	31.090270192773243	33.0	32.0	33.0	27.0	33.0
6	35.032822805192936	37.0	35.0	38.0	29.0	38.0
7	35.41857069090068	38.0	35.0	38.0	29.0	38.0
8	36.08209160441025	38.0	37.0	38.0	33.0	38.0
9	36.27911643703071	38.0	37.0	38.0	33.0	38.0
10-11	36.32693125410569	38.0	37.0	38.0	33.0	38.0
12-13	36.30785250432692	38.0	37.0	38.0	33.0	38.0
14-15	36.41145994970755	38.0	37.0	38.0	33.0	38.0
16-17	36.40575448527357	38.0	37.0	38.0	33.0	38.0
18-19	36.432639285702244	38.0	38.0	38.0	34.0	38.0
20-21	36.44247593031955	38.0	38.0	38.0	34.0	38.0
22-23	36.43934353498568	38.0	38.0	38.0	34.0	38.0
24-25	36.43415801305164	38.0	38.0	38.0	34.0	38.0
26-27	36.370991578689804	38.0	37.5	38.0	33.0	38.0
28-29	36.34481650204362	38.0	37.0	38.0	33.0	38.0
30-31	36.34214540845743	38.0	37.0	38.0	33.0	38.0
32-33	36.31855350109293	38.0	37.0	38.0	33.0	38.0
34-35	36.29287622436334	38.0	37.0	38.0	33.0	38.0
36-37	36.26910171371872	38.0	37.0	38.0	33.0	38.0
38-39	36.258219720448416	38.0	37.0	38.0	33.0	38.0
40-41	36.246770071428585	38.0	37.0	38.0	33.0	38.0
42-43	36.196393508045404	38.0	37.0	38.0	33.0	38.0
44-45	36.14654117895411	38.0	37.0	38.0	33.0	38.0
46-47	36.111078708341964	38.0	37.0	38.0	33.0	38.0
48-49	36.06066388415157	38.0	37.0	38.0	32.5	38.0
50-51	36.028362937256375	38.0	37.0	38.0	31.0	38.0
52-53	36.007169152841776	38.0	37.0	38.0	31.0	38.0
54-55	35.96935035420877	38.0	37.0	38.0	31.0	38.0
56-57	35.937845081585465	38.0	37.0	38.0	31.0	38.0
58-59	35.9134528853849	38.0	37.0	38.0	31.0	38.0
60-61	35.90989461599322	38.0	37.0	38.0	31.0	38.0
62-63	35.90274744542458	38.0	37.0	38.0	31.0	38.0
64-65	35.87351371743553	38.0	37.0	38.0	31.0	38.0
66-67	35.85076592683174	38.0	37.0	38.0	31.0	38.0
68-69	35.84736649228607	38.0	37.0	38.0	31.0	38.0
70-71	35.81580880396423	38.0	37.0	38.0	31.0	38.0
72-73	35.804948787264934	38.0	37.0	38.0	31.0	38.0
74-75	35.817712951165106	38.0	37.0	38.0	31.0	38.0
76-77	35.775770461954764	38.0	37.0	38.0	31.0	38.0
78-79	35.77524500409515	38.0	37.0	38.0	31.0	38.0
80-81	35.75101698006672	38.0	37.0	38.0	31.0	38.0
82-83	35.73168619105034	38.0	37.0	38.0	30.5	38.0
84-85	35.70372966950505	38.0	36.5	38.0	29.5	38.0
86-87	35.67498445464674	38.0	36.0	38.0	29.0	38.0
88-89	35.59565790842126	38.0	36.0	38.0	29.0	38.0
90-91	35.615006399380604	38.0	36.0	38.0	29.0	38.0
92-93	35.65109470967407	38.0	36.0	38.0	29.0	38.0
94-95	35.621703075466584	38.0	36.0	38.0	29.0	38.0
96-97	35.619190220074444	38.0	36.0	38.0	29.0	38.0
98-99	35.643509997328835	38.0	36.0	38.0	30.0	38.0
100-101	35.05292144574811	38.0	35.0	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	63.0
19	109.0
20	278.0
21	1402.0
22	5936.0
23	18010.0
24	41211.0
25	76631.0
26	125120.0
27	183960.0
28	257324.0
29	338806.0
30	434518.0
31	549249.0
32	692225.0
33	899445.0
34	1226789.0
35	1884520.0
36	3984119.0
37	7666336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.023231080854856	28.434376743508587	13.538676969255725	24.003715206380836
2	13.985172895679977	21.30393040204703	39.02794715430573	25.682949547967272
3	16.389883716895234	26.31716068067448	32.85909018372267	24.433865418707615
4	13.318887531875538	19.35986498397011	39.0558831151914	28.26536436896296
5	13.6872794597866	36.91362817307733	35.63247475082214	13.766617616313928
6	28.66563349250829	39.88113167052992	18.719205606770085	12.734029230191704
7	25.147217527541795	33.83136287288988	23.901866294558626	17.1195533050097
8	23.55085576255661	37.72154966551286	21.31302424913486	17.414570322795676
9	24.780507289446685	17.14436263354703	21.79634518203819	36.27878489496809
10-11	23.209684207457627	27.525829625904585	29.57315537675341	19.69133078988438
12-13	24.113957002473203	25.036280888385125	29.671649291861506	21.17811281728016
14-15	21.53196850753441	26.470311489974563	27.16829965868024	24.82942034381079
16-17	20.9755737399314	29.66308292658253	27.961621995803615	21.399721337682465
18-19	20.991881457134532	28.858894059835993	29.63603726258233	20.513187220447147
20-21	21.803282593911007	27.94068967932911	29.53408528713396	20.721942439625916
22-23	21.4994512867999	27.771170277759406	29.44558318509519	21.283795250345502
24-25	21.29925213641215	27.921402912572113	29.519759685778762	21.25958526523698
26-27	21.29778977863238	28.0854409826676	29.371533373802666	21.24523586489735
28-29	21.242230724157086	28.147561569391815	29.4078663132124	21.2023413932387
30-31	21.526110289876204	27.958402092027562	29.33957033015096	21.17591728794527
32-33	21.37302399241297	28.060322920378095	29.271517218403094	21.295135868805843
34-35	21.46634067493224	28.090085297194424	29.284730809909554	21.15884321796379
36-37	21.43484704399018	27.98397001060361	29.33811080059636	21.243072144809847
38-39	21.31014313355626	28.127702439887575	29.336046251940346	21.226108174615817
40-41	21.530368051267164	27.987230963929623	29.22896580391992	21.253435180883294
42-43	21.4164120203136	28.09869550665824	29.045113891035385	21.439778581992776
44-45	21.500543214198625	28.125392495299906	28.926943353896345	21.447120936605124
46-47	21.57023881668113	28.105139787690884	28.733002760961607	21.59161863466638
48-49	21.457368511593767	28.20665312447922	28.84151538782394	21.494462976103076
50-51	21.49405951485906	28.257696997776822	28.665174901067324	21.583068586296793
52-53	21.625241541757628	28.203611357643165	28.555262490371707	21.615884610227496
54-55	21.575336572843266	28.127373033149638	28.614485629028835	21.682804764978258
56-57	21.558056851121524	28.14933746659087	28.644195609473066	21.648410072814535
58-59	21.552332058257686	28.178152202259643	28.68150788209316	21.588007857389513
60-61	21.61641824923624	28.14925922294485	28.70257825583736	21.53174427198155
62-63	21.600406038155445	28.177945330344418	28.660305337994966	21.56134329350517
64-65	21.51721258197669	28.142920840344924	28.669932666564733	21.66993391111365
66-67	21.57604546252839	28.29328626495412	28.4702978833681	21.660370389149385
68-69	21.52625539540724	28.23194414810079	28.50011657185129	21.74168388464068
70-71	21.565381746833495	28.017275676669083	28.550094381222284	21.867248195275135
72-73	21.576242846004853	28.081444558147084	28.495345114096537	21.846967481751523
74-75	21.482898684127097	28.17600449717294	28.46403792812376	21.877058890576205
76-77	21.66171373125845	28.110932070116196	28.390255050143704	21.837099148481656
78-79	21.65690123937274	28.155128659241413	28.314077002325593	21.873893099060254
80-81	21.648025671179433	28.180291213709697	28.26060366012265	21.911079454988226
82-83	21.680466124830826	28.131070505081567	28.356250224691887	21.83221314539572
84-85	21.784498694042043	28.1908863741066	28.274196909030135	21.75041802282122
86-87	21.689514520717697	28.22828222684519	28.291919860539423	21.79028339189769
88-89	21.707030349990017	28.238379604424413	28.296785799010465	21.75780424657511
90-91	21.758227536278827	28.275864307486675	28.338785565168738	21.627122591065763
92-93	21.840588839934284	28.187655724236333	28.287292678679105	21.68446275715028
94-95	21.678740936692254	28.28944091750586	28.314110150282108	21.717707995519778
96-97	21.683177438724734	28.30944633143424	28.32906365865736	21.678312571183668
98-99	21.830643526583636	28.828376729326916	28.44644529355154	20.894534450537904
100-101	22.385032360749857	31.341570364486497	25.94922860592114	20.324168668842507
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4562.0
1	4359.5
2	5155.0
3	6235.5
4	7135.5
5	9078.5
6	11385.5
7	15044.0
8	18802.0
9	20944.5
10	20986.5
11	20724.0
12	22996.5
13	28829.0
14	41365.0
15	56642.0
16	67659.0
17	73044.0
18	73282.5
19	70248.5
20	66810.5
21	65554.0
22	69684.5
23	82155.5
24	102147.5
25	132107.5
26	172702.0
27	217415.5
28	265306.0
29	316085.0
30	362531.0
31	410769.5
32	462104.0
33	511796.0
34	556476.0
35	593645.0
36	634321.5
37	672758.0
38	697794.5
39	713328.5
40	733072.5
41	753115.5
42	764010.5
43	770556.5
44	773522.5
45	771591.5
46	763830.5
47	754640.0
48	740457.0
49	716435.5
50	689230.0
51	659348.5
52	620767.5
53	577140.5
54	533897.5
55	488132.5
56	440079.0
57	391828.0
58	343920.0
59	292464.5
60	238075.0
61	188356.0
62	145209.5
63	109128.0
64	80203.0
65	58249.0
66	42131.0
67	30850.0
68	22792.5
69	16310.0
70	11568.0
71	8229.5
72	5786.5
73	3921.5
74	2618.0
75	1721.0
76	1050.5
77	609.5
78	385.0
79	233.0
80	153.5
81	96.0
82	63.5
83	48.0
84	33.5
85	22.0
86	14.0
87	9.5
88	6.0
89	6.0
90	5.0
91	2.5
92	1.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.002240828349393872
4	5.058180497418207E-4
5	0.049080667536238604
6	0.0
7	0.0
8	0.0
9	1.4141149777728318E-4
10-11	0.0
12-13	2.7194518803323693E-6
14-15	3.9703997452852593E-4
16-17	2.991397068365606E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.115331052290909E-4
28-29	0.0
30-31	2.7238799861746748E-5
32-33	0.0
34-35	3.5565905538049224E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.173355379004683E-4
44-45	2.3076524541447646E-4
46-47	1.2484549660445786E-4
48-49	0.002045188131421444
50-51	6.282818178268048E-4
52-53	0.005441467727815752
54-55	0.0011831703051247225
56-57	0.0030068779833040014
58-59	0.008318820099099166
60-61	0.0089556451213003
62-63	0.007352517442357256
64-65	0.010829438140036715
66-67	0.00477311229608531
68-69	0.004489630097738598
70-71	0.0049405115930944744
72-73	0.007366028468503838
74-75	0.005469956628516948
76-77	0.0026745328290246205
78-79	1.4900372706909572E-4
80-81	3.2731052509216493E-4
82-83	0.0
84-85	0.0
86-87	7.933962155540703E-4
88-89	2.7306962135278826E-5
90-91	8.632587444398799E-5
92-93	1.397726597688859E-5
94-95	7.605168783986925E-4
96-97	3.731152924100959E-4
98-99	4.4468783692051895E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	205.0
20-21	715.0
22-23	1583.0
24-25	2400.0
26-27	5300.0
28-29	12056.0
30-31	32411.0
32-33	42352.0
34-35	58419.0
36-37	90940.0
38-39	104296.0
40-41	109034.0
42-43	124068.0
44-45	143594.0
46-47	144425.0
48-49	134039.0
50-51	124053.0
52-53	111792.0
54-55	119194.0
56-57	120434.0
58-59	133261.0
60-61	133111.0
62-63	130286.0
64-65	140425.0
66-67	148255.0
68-69	151069.0
70-71	148944.0
72-73	142910.0
74-75	146766.0
76-77	154101.0
78-79	159174.0
80-81	160000.0
82-83	153671.0
84-85	153668.0
86-87	159486.0
88-89	166946.0
90-91	171625.0
92-93	171436.0
94-95	186762.0
96-97	292724.0
98-99	1536178.0
100-101	1.216395E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.75233393606787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.90879224054436	71.86748939165395
2	7.980932189444899	13.049196669452254
3	2.152025888065629	5.277994171206131
4	0.8535255668285063	2.7911082864934267
5	0.4129964928651793	1.6881713599569506
6	0.23073459844190933	1.1317855165456496
7	0.13107753712083825	0.7501126210354072
8	0.08723796767976755	0.5705525972528203
9	0.05570642146534737	0.40987169730165757
>10	0.184217819422164	2.2227148254339926
>50	0.002077360307097893	0.10826787864169664
>100	6.268960259771614E-4	0.09042012740113503
>500	3.677106149885151E-5	0.025599396849455478
>1k	1.2250726830562936E-5	0.016715460775447732
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6316711281994215E-5
2	0.0	2.1755615042658954E-5	0.0	5.4389037606647386E-6	1.6316711281994215E-5
3	0.0	4.351123008531791E-5	0.0	5.4389037606647386E-6	1.6316711281994215E-5
4	0.0	7.07057488886416E-5	0.0	5.4389037606647386E-6	2.7194518803323694E-5
5	0.0	7.614465264930634E-5	0.0	1.0877807521329477E-5	2.7194518803323694E-5
6	0.0	7.614465264930634E-5	0.0	1.0877807521329477E-5	2.7194518803323694E-5
7	0.0	7.614465264930634E-5	0.0	1.0877807521329477E-5	2.7194518803323694E-5
8	0.0	8.158355640997107E-5	0.0	1.0877807521329477E-5	3.807232632465317E-5
9	0.0	1.4685040153794793E-4	0.0	1.0877807521329477E-5	3.807232632465317E-5
10-11	5.4389037606647386E-6	1.4685040153794793E-4	0.0	2.7194518803323694E-5	3.807232632465317E-5
12-13	1.6316711281994215E-5	1.4685040153794793E-4	0.0	2.9913970683656063E-5	4.079177820498554E-5
14-15	2.1755615042658954E-5	1.686060165806069E-4	0.0	3.807232632465317E-5	6.254739324764449E-5
16-17	2.9913970683656063E-5	1.7404492034127163E-4	0.0	4.079177820498554E-5	9.246136393130055E-5
18-19	3.263342256398843E-5	1.9036163162326585E-4	0.0	4.351123008531791E-5	9.79002676919653E-5
20-21	3.807232632465317E-5	2.0667834290526006E-4	0.0	4.351123008531791E-5	1.0333917145263004E-4
22-23	5.438903760664739E-5	2.583479286315751E-4	0.0	4.6230681965650275E-5	1.1421697897395951E-4
24-25	8.430300829030344E-5	2.773840917939017E-4	0.0	7.342520076897396E-5	1.3053369025595372E-4
26-27	8.702246017063582E-5	2.828229955545664E-4	0.0	1.5772820905927742E-4	1.3053369025595372E-4
28-29	8.702246017063582E-5	2.828229955545664E-4	0.0	5.30293116664812E-4	1.3053369025595372E-4
30-31	9.518081581163292E-5	2.991397068365606E-4	0.0	0.0019471275463179764	1.3053369025595372E-4
32-33	1.0333917145263003E-4	2.991397068365606E-4	0.0	0.00499291365229023	1.4413094965761558E-4
34-35	1.1421697897395951E-4	3.508092925628756E-4	0.0	0.009131919414156097	1.5228930529861268E-4
36-37	1.2509478649528897E-4	4.024788782891906E-4	0.0	0.015188138751656283	1.5228930529861268E-4
38-39	1.2509478649528897E-4	4.0791778204985537E-4	0.0	0.027330491397340312	1.5228930529861268E-4
40-41	1.3597259401661846E-4	4.2967339709251435E-4	0.0	0.06337410661926554	1.5228930529861268E-4
42-43	1.3597259401661846E-4	4.4870956025484094E-4	0.0	0.075217319558113	1.5500875717894505E-4
44-45	1.4141149777728318E-4	5.221347610238149E-4	0.0	0.0923553053079676	1.6044766093960977E-4
46-47	1.4685040153794793E-4	5.710848948697975E-4	0.0	0.11190000597191632	1.7132546846093926E-4
48-49	1.4685040153794793E-4	5.710848948697975E-4	0.0	0.1292392311609155	1.849227278626011E-4
50-51	1.4685040153794793E-4	6.091572211944507E-4	0.0	0.14610255227085656	1.849227278626011E-4
52-53	1.5228930529861268E-4	6.091572211944507E-4	0.0	0.17607635089587992	1.958005353839306E-4
54-55	1.6316711281994214E-4	6.091572211944507E-4	0.0	0.24940093194528157	2.0667834290526006E-4
56-57	1.6316711281994214E-4	6.145961249551154E-4	0.0	0.3315501343463618	2.0667834290526006E-4
58-59	1.6316711281994214E-4	6.173155768354478E-4	0.0	0.4184175857598187	2.0667834290526006E-4
60-61	1.6316711281994214E-4	6.254739324764449E-4	0.0	0.44382270522588363	2.0667834290526006E-4
62-63	1.6316711281994214E-4	6.363517399977744E-4	0.0	0.4584451979864308	2.2843395794791901E-4
64-65	1.6316711281994214E-4	6.961796813650865E-4	0.0	0.4686621787008395	2.2843395794791901E-4
66-67	1.6316711281994214E-4	7.07057488886416E-4	0.0	0.4746395339338101	2.3387286170858376E-4
68-69	1.686060165806069E-4	7.152158445274131E-4	0.0	0.48047003876524264	2.3931176546924848E-4
70-71	1.7404492034127163E-4	7.179352964077455E-4	0.0	0.4856070833671905	2.583479286315751E-4
72-73	1.7404492034127163E-4	7.315325558094073E-4	0.0	0.48989022007871397	3.1545641811855484E-4
74-75	1.7404492034127163E-4	7.50568718971734E-4	0.0	0.49349077436827404	3.208953218792196E-4
76-77	1.7404492034127163E-4	7.940799490570517E-4	0.0	0.49677587223971553	3.2361477375955194E-4
78-79	1.7404492034127163E-4	8.049577565783812E-4	0.0	0.4996041021952612	3.2905367752021663E-4
80-81	1.7404492034127163E-4	8.049577565783812E-4	0.0	0.5009583892316667	3.508092925628756E-4
82-83	1.76764372221604E-4	8.10396660339046E-4	0.0	0.501434293310725	3.807232632465317E-4
84-85	1.7948382410193638E-4	8.10396660339046E-4	0.0	0.5019455502642274	3.807232632465317E-4
86-87	1.7948382410193638E-4	8.239939197407079E-4	0.0	0.5021767036740556	3.916010707678612E-4
88-89	1.7948382410193638E-4	8.8382186110802E-4	0.0	0.502261006682346	3.916010707678612E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21230	0.0	48.20781	1
GTATCAA	37425	0.0	33.09361	1
ATCAACG	46455	0.0	29.483398	3
TCAACGC	46930	0.0	29.147146	4
CAACGCA	47875	0.0	28.553658	5
TATCAAC	47990	0.0	28.233753	2
AACGCAG	50035	0.0	27.325216	6
ACGCAGA	59610	0.0	22.892502	7
CGCAGAG	60485	0.0	22.568604	8
GCAGAGT	71645	0.0	19.071249	9
GAGTACT	40790	0.0	18.832354	12-13
ACCGAAC	1905	0.0	18.618052	8
TGGTATC	10495	0.0	17.81302	2
GTGGTAT	10355	0.0	17.803106	1
CAGAGTA	70035	0.0	17.761887	10-11
GTACTTT	45885	0.0	16.78869	14-15
AGAGTAC	65095	0.0	16.498499	10-11
CCGAACC	2145	0.0	15.526687	9
GGACCGA	2585	0.0	15.058959	6
TACATGG	47515	0.0	14.408277	2
>>END_MODULE