FastQCFastQC Report
Fri 27 May 2016
SRR2140396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206070
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182150.055.9529041
GTATCAA333800.037.786991
ATCAACG383600.032.38543
TCAACGC384450.032.2797664
CAACGCA392700.031.5684455
TATCAAC401000.031.0017132
AACGCAG402500.030.7414786
ACGCAGA449500.027.5175177
GTGGTAT76300.027.2591781
CGCAGAG454350.027.2242648
TGGTATC73050.026.2817922
GCAGAGT522950.023.5703729
GAGTACT329250.022.59082812-13
GTACATG348500.021.7009751
CAGAGTA517650.021.51986310-11
TACATGG347600.021.1476142
ACATGGG342700.020.7433993
GTACTTT367200.020.19758414-15
AGAGTAC483150.018.86564410-11
CATGGGG247450.018.3655364