##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140396_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11206070
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.628326166086772	32.0	18.0	33.0	18.0	33.0
2	29.430782781117735	31.0	27.0	33.0	18.0	33.0
3	30.45211363127305	33.0	29.0	33.0	25.0	33.0
4	30.18904504433758	33.0	30.0	33.0	25.0	33.0
5	30.853321191104463	33.0	32.0	33.0	27.0	33.0
6	33.82774255381236	37.0	33.0	38.0	26.0	38.0
7	35.245256365523325	37.0	35.0	38.0	29.0	38.0
8	35.069032229853995	38.0	35.0	38.0	29.0	38.0
9	35.78637773992131	38.0	36.0	38.0	31.0	38.0
10-11	36.03881293798807	38.0	37.0	38.0	32.0	38.0
12-13	36.096192509952196	38.0	37.0	38.0	32.0	38.0
14-15	36.17539413906927	38.0	37.0	38.0	33.0	38.0
16-17	36.17413620475332	38.0	37.0	38.0	33.0	38.0
18-19	36.22522266949966	38.0	37.0	38.0	33.0	38.0
20-21	36.25720822635744	38.0	37.0	38.0	33.0	38.0
22-23	36.26522536428848	38.0	37.0	38.0	33.0	38.0
24-25	36.2602503166467	38.0	37.0	38.0	33.0	38.0
26-27	36.20217880315205	38.0	37.0	38.0	33.0	38.0
28-29	36.180079907563396	38.0	37.0	38.0	33.0	38.0
30-31	36.18103680704378	38.0	37.0	38.0	33.0	38.0
32-33	36.157969183202965	38.0	37.0	38.0	33.0	38.0
34-35	36.12425393681703	38.0	37.0	38.0	33.0	38.0
36-37	36.09607268103125	38.0	37.0	38.0	33.0	38.0
38-39	36.074393547050164	38.0	37.0	38.0	32.0	38.0
40-41	36.07338658408051	38.0	37.0	38.0	32.0	38.0
42-43	36.029729521614435	38.0	37.0	38.0	31.5	38.0
44-45	35.97073194799836	38.0	37.0	38.0	31.0	38.0
46-47	35.92376174185765	38.0	37.0	38.0	31.0	38.0
48-49	35.86373708878311	38.0	37.0	38.0	31.0	38.0
50-51	35.82254102348503	38.0	37.0	38.0	31.0	38.0
52-53	35.7990658005581	38.0	37.0	38.0	31.0	38.0
54-55	35.76141616434444	38.0	37.0	38.0	31.0	38.0
56-57	35.72638971340702	38.0	37.0	38.0	29.5	38.0
58-59	35.69595256229501	38.0	37.0	38.0	29.0	38.0
60-61	35.69398583689313	38.0	37.0	38.0	29.0	38.0
62-63	35.69111971365061	38.0	37.0	38.0	29.0	38.0
64-65	35.65709858807716	38.0	36.0	38.0	29.0	38.0
66-67	35.636373554382274	38.0	36.0	38.0	29.0	38.0
68-69	35.62935383679775	38.0	36.0	38.0	29.0	38.0
70-71	35.59469825754114	38.0	36.0	38.0	29.0	38.0
72-73	35.582899725159464	38.0	36.0	38.0	29.0	38.0
74-75	35.597152074802615	38.0	36.0	38.0	29.0	38.0
76-77	35.56092937634732	38.0	36.0	38.0	29.0	38.0
78-79	35.55991020710168	38.0	36.0	38.0	29.0	38.0
80-81	35.532500513862836	38.0	36.0	38.0	29.0	38.0
82-83	35.512210457350875	38.0	36.0	38.0	29.0	38.0
84-85	35.48482780223309	38.0	36.0	38.0	29.0	38.0
86-87	35.45401895859001	38.0	36.0	38.0	29.0	38.0
88-89	35.37467806404516	38.0	36.0	38.0	29.0	38.0
90-91	35.39850823554173	38.0	36.0	38.0	29.0	38.0
92-93	35.43393483357592	38.0	36.0	38.0	29.0	38.0
94-95	35.40657058740774	38.0	36.0	38.0	29.0	38.0
96-97	35.40639539947776	38.0	36.0	38.0	29.0	38.0
98-99	35.43630223237715	38.0	36.0	38.0	29.0	38.0
100-101	34.84898174455182	37.5	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	12.0
19	45.0
20	256.0
21	1224.0
22	5165.0
23	14610.0
24	31267.0
25	56914.0
26	91318.0
27	132489.0
28	181253.0
29	236306.0
30	297674.0
31	371774.0
32	462105.0
33	587972.0
34	782324.0
35	1154904.0
36	2211578.0
37	4586879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.591668622451934	18.47418408059204	13.883100855161533	21.05104644179449
2	15.02254581668685	20.85971263788286	43.380409010473784	20.7373325349565
3	18.162670530876046	28.091464179703586	29.809154985006792	23.936710304413577
4	12.32504430670053	20.38312579533428	38.513409804408006	28.778420093557187
5	14.1575668249768	37.73360149094968	35.30528166102965	12.803550023043867
6	28.791083760854608	38.97342243980271	19.06431960535674	13.171174193985937
7	25.75554141639308	33.96302182656364	23.051703228696592	17.229733528346692
8	23.87538182431486	37.35938647536558	21.063664603201655	17.701567097117902
9	25.127188003550582	17.8073467285392	21.551476349960634	35.51398891794958
10-11	23.59230756188387	27.50225101217465	28.94315313040165	19.962288295539828
12-13	24.72327497508047	25.05935176203611	29.125366877058596	21.092006385824824
14-15	21.850578693468215	26.440460256224803	27.053943063990875	24.655017986316103
16-17	21.02348290020345	29.804247145006872	27.67544979421419	21.49682016057549
18-19	21.03978022625238	28.54714453862951	29.807184856064616	20.605890379053495
20-21	21.8845248181995	27.573885337614584	29.90289769318159	20.638692151004324
22-23	21.650944762275852	27.524373127763468	29.736173032172335	21.08850907778834
24-25	21.38957244954519	27.69995153339242	29.820695866235734	21.089780150826655
26-27	21.326944405895055	27.814505713748915	29.775584809646077	21.08296507070995
28-29	21.236213750810062	27.815593108804553	29.81675166817545	21.131441472209936
30-31	21.568902400020885	27.65120937956932	29.711711145511266	21.068177074898532
32-33	21.318319023292105	27.837664561145054	29.6894132445708	21.154603170992043
34-35	21.490458997168812	27.7498210865546	29.68997465130959	21.069745264966997
36-37	21.354425906755083	27.789127076973575	29.68066893214059	21.17577808413075
38-39	21.209635973993638	27.823570014326766	29.793640916057758	21.17315309562184
40-41	21.492677636102943	27.684040540050425	29.64531283233042	21.177968991516217
42-43	21.43806802560887	27.76546958189794	29.481369337763734	21.315093054729456
44-45	21.434978059121867	27.890634810297403	29.330778961926796	21.343608168653933
46-47	21.577380862221663	27.879160354933813	28.99767639369889	21.545782389145636
48-49	21.487680181108598	27.99567345834874	28.95240752480201	21.56423883574065
50-51	21.441255793247237	28.131915741441382	28.904740299475794	21.52208816583559
52-53	21.62641578950833	28.02499778430725	28.848892043912404	21.49969438227202
54-55	21.567670143616237	27.937601250889653	28.83624377314079	21.65848483235332
56-57	21.602931458227538	28.05348028258823	28.78391858375104	21.5596696754332
58-59	21.512006254359296	28.12950273783984	28.887631085481907	21.470859922318958
60-61	21.60526772337495	28.127935219017136	28.801845805058086	21.464951252549827
62-63	21.471652146081695	28.27152305370142	28.809765682296057	21.447059117920826
64-65	21.45273451680454	28.256450976249585	28.722394282376218	21.56842022456966
66-67	21.50307837997089	28.31569475678437	28.586095545869895	21.59513131737484
68-69	21.46173883843759	28.25427557112737	28.665308081483637	21.618677508951407
70-71	21.52020105696409	28.036828656564598	28.67049441884407	21.772475867627243
72-73	21.53014986663964	27.997383261997477	28.6378815478819	21.834585323480983
74-75	21.429362595038544	28.12582168382053	28.634847222099648	21.80996849904128
76-77	21.695262410576046	28.058522958863424	28.52537052092329	21.720844109637238
78-79	21.66733277738367	28.09114310453007	28.412158800085365	21.8293653180009
80-81	21.597893459717437	28.140014963142214	28.419694738563877	21.842396838576473
82-83	21.66344074366558	28.098324503133153	28.492650464519333	21.74558428868194
84-85	21.817529891103487	28.114196112725654	28.43024355567614	21.638030440494724
86-87	21.612655100301122	28.187320265249383	28.499668898364494	21.700355736085
88-89	21.72182276634254	28.233397773548997	28.43302704388051	21.611752416227954
90-91	21.694081501050015	28.2596680073756	28.453634477185847	21.59261601438854
92-93	21.721307456974912	28.23412001583705	28.42234698567068	21.62222554151736
94-95	21.66131707549801	28.2775721016586	28.441571285841594	21.619539537001796
96-97	21.69400500845681	28.239196928177883	28.46558105956909	21.601217003796222
98-99	21.777893555826637	28.88668399810547	28.590641229791515	20.744781216276376
100-101	22.384556806711906	31.324023842852018	26.09196615936496	20.199453191071118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2769.0
1	2473.5
2	3015.0
3	3993.0
4	4709.5
5	6996.5
6	9711.5
7	13349.0
8	18289.0
9	20865.0
10	19046.0
11	16423.0
12	16786.0
13	20075.0
14	27759.5
15	38090.5
16	47480.5
17	55195.5
18	58842.0
19	57384.5
20	53687.5
21	50110.5
22	49147.0
23	53512.0
24	63834.0
25	80226.5
26	101740.0
27	125661.0
28	152990.0
29	183072.0
30	210932.5
31	239569.0
32	269907.5
33	298535.5
34	325250.5
35	348784.0
36	373883.0
37	396646.0
38	413782.0
39	427912.5
40	440857.5
41	452810.0
42	459082.0
43	464084.5
44	473409.5
45	476908.5
46	468004.0
47	456199.5
48	445514.5
49	432860.5
50	417619.0
51	398776.5
52	376159.5
53	351913.5
54	326947.5
55	300625.0
56	272207.0
57	243085.5
58	214780.0
59	184687.0
60	152397.5
61	122453.0
62	94994.0
63	71583.0
64	53507.0
65	39395.0
66	28657.0
67	21332.0
68	15942.0
69	11600.5
70	8441.0
71	5958.0
72	4154.0
73	2866.0
74	1882.5
75	1160.5
76	704.5
77	423.5
78	267.0
79	179.5
80	124.0
81	87.5
82	56.5
83	33.5
84	22.5
85	20.5
86	19.0
87	16.5
88	10.0
89	4.5
90	4.5
91	5.0
92	4.5
93	3.5
94	2.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0029269851071785204
4	8.031361574575208E-4
5	0.05079390009164676
6	0.0
7	0.0
8	0.0
9	1.8739843674008818E-4
10-11	0.0
12-13	0.0
14-15	4.5511048922592843E-4
16-17	8.923735082861343E-6
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.0266601317517411E-4
28-29	0.0
30-31	1.788001091217066E-5
32-33	0.0
34-35	2.6938027182804058E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.1921496760424959E-4
44-45	2.5409977278398316E-4
46-47	1.0253181340794544E-4
48-49	0.001983491615494228
50-51	6.821250735060821E-4
52-53	0.0051323208669768255
54-55	0.0011376307615357918
56-57	0.002769334716872981
58-59	0.007855786451104666
60-61	0.008624605085808933
62-63	0.007185019595125877
64-65	0.010333176399982156
66-67	0.004762085791565265
68-69	0.004350193743517855
70-71	0.004649892568000321
72-73	0.006858165984594023
74-75	0.005258499918642076
76-77	0.002561266441907631
78-79	1.8626230362298805E-4
80-81	2.7960371550321946E-4
82-83	0.0
84-85	0.0
86-87	7.760567426082535E-4
88-89	1.680805226721576E-5
90-91	7.366527169197173E-5
92-93	1.1467812973961128E-5
94-95	6.790690207493892E-4
96-97	2.648724403757422E-4
98-99	5.472698894295916E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	181.0
20-21	610.0
22-23	1286.0
24-25	1942.0
26-27	3717.0
28-29	7921.0
30-31	19846.0
32-33	25983.0
34-35	35478.0
36-37	55719.0
38-39	63466.0
40-41	67103.0
42-43	77766.0
44-45	92708.0
46-47	92751.0
48-49	85072.0
50-51	75953.0
52-53	65006.0
54-55	69846.0
56-57	70901.0
58-59	80573.0
60-61	81419.0
62-63	79184.0
64-65	86084.0
66-67	92486.0
68-69	93512.0
70-71	98307.0
72-73	85056.0
74-75	86336.0
76-77	90990.0
78-79	95399.0
80-81	97079.0
82-83	92351.0
84-85	90806.0
86-87	94458.0
88-89	99444.0
90-91	103616.0
92-93	104110.0
94-95	112666.0
96-97	179558.0
98-99	937012.0
100-101	7412369.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.72608762398161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.84602000979199	74.38729656381867
2	7.644287252578258	12.800525286645058
3	1.9475985593286358	4.891944229040692
4	0.7034568859344107	2.3559077148575085
5	0.3367045559863636	1.4095477578954052
6	0.1806742735469528	0.9076290035034837
7	0.104614098246167	0.613125040952368
8	0.06268353645977012	0.4198597812969408
9	0.0397584375620569	0.299593658440204
>10	0.13129809569354414	1.6685468637765613
>50	0.0023260792139769048	0.12830756099814483
>100	5.241189337914184E-4	0.07635089177192733
>500	3.6066538727364744E-5	0.022661741745744507
>1k	1.803018528519775E-5	0.018703905257276474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7847470165722683E-5	0.0	0.0	0.0
3	0.0	1.7847470165722683E-5	0.0	0.0	0.0
4	0.0	5.354241049716805E-5	0.0	1.7847470165722683E-5	0.0
5	0.0	6.246614558002939E-5	0.0	1.7847470165722683E-5	0.0
6	0.0	6.246614558002939E-5	0.0	1.7847470165722683E-5	8.923735082861342E-6
7	0.0	6.246614558002939E-5	0.0	1.7847470165722683E-5	8.923735082861342E-6
8	0.0	7.138988066289073E-5	0.0	1.7847470165722683E-5	8.923735082861342E-6
9	0.0	1.3385602624292012E-4	0.0	8.031361574575208E-5	8.923735082861342E-6
10-11	0.0	1.3385602624292012E-4	0.0	8.923735082861342E-5	8.923735082861342E-6
12-13	0.0	1.3831789378435078E-4	0.0	9.816108591147476E-5	8.923735082861342E-6
14-15	0.0	1.8293656919865753E-4	0.0	9.816108591147476E-5	2.2309337707153355E-5
16-17	0.0	1.9632217182294953E-4	0.0	1.070848209943361E-4	3.1233072790014695E-5
18-19	0.0	2.2309337707153356E-4	0.0	1.2939415870148947E-4	4.461867541430671E-5
20-21	0.0	2.4986458232011756E-4	0.0	1.695509665743655E-4	4.461867541430671E-5
22-23	0.0	2.900213901929936E-4	0.0	1.8739843674008818E-4	4.461867541430671E-5
24-25	0.0	3.034069928172856E-4	0.0	2.3201711215439487E-4	4.461867541430671E-5
26-27	4.461867541430671E-6	3.034069928172856E-4	0.0	3.034069928172856E-4	4.461867541430671E-5
28-29	8.923735082861342E-6	3.034069928172856E-4	0.0	7.674412171260754E-4	4.461867541430671E-5
30-31	1.3385602624292012E-5	3.4802566823159236E-4	0.0	0.002047997201516678	4.908054295573738E-5
32-33	2.6771205248584027E-5	3.569494033144537E-4	0.0	0.004671575315877913	7.138988066289073E-5
34-35	3.569494033144537E-5	4.5957235676735914E-4	0.0	0.00845077712346969	8.031361574575208E-5
36-37	3.569494033144537E-5	5.621953102202645E-4	0.0	0.01455907378768828	8.031361574575208E-5
38-39	3.569494033144537E-5	5.711190453031259E-4	0.0	0.026824747659081195	9.369921837004409E-5
40-41	4.461867541430671E-5	6.514326610488779E-4	0.0	0.06557606725640658	9.816108591147476E-5
42-43	4.461867541430671E-5	6.603563961317394E-4	0.0	0.07716799912904346	1.070848209943361E-4
44-45	4.461867541430671E-5	7.228225417117687E-4	0.0	0.09470313856686599	1.2047042361862812E-4
46-47	4.461867541430671E-5	7.763649522089368E-4	0.0	0.11445136430523814	1.3831789378435078E-4
48-49	4.461867541430671E-5	7.763649522089368E-4	0.0	0.13278071616543535	1.4277976132578147E-4
50-51	4.461867541430671E-5	8.522167004132582E-4	0.0	0.15065049566886518	1.4277976132578147E-4
52-53	5.354241049716805E-5	8.566785679546889E-4	0.0	0.1826287003382988	1.4724162886721215E-4
54-55	5.354241049716805E-5	8.745260381204116E-4	0.0	0.25990824615587804	1.6508909903293484E-4
56-57	5.354241049716805E-5	9.012972433689955E-4	0.0	0.3463569297710973	1.7847470165722684E-4
58-59	5.354241049716805E-5	9.057591109104261E-4	0.0	0.43960103765191544	1.7847470165722684E-4
60-61	5.354241049716805E-5	9.102209784518569E-4	0.0	0.46450272040064	1.7847470165722684E-4
62-63	5.354241049716805E-5	9.102209784518569E-4	0.0	0.47883870081125673	1.8739843674008818E-4
64-65	5.354241049716805E-5	0.0010083820643633317	0.0	0.4884317160253327	1.8739843674008818E-4
66-67	5.354241049716805E-5	0.001017305799446193	0.0	0.49407597846524254	1.9186030428151884E-4
68-69	5.354241049716805E-5	0.0010530007397776384	0.0	0.4991803549326391	1.9632217182294953E-4
70-71	5.354241049716805E-5	0.001070848209943361	0.0	0.5042178033869145	2.007840393643802E-4
72-73	5.354241049716805E-5	0.001070848209943361	0.0	0.5082067129689534	2.141696419886722E-4
74-75	5.354241049716805E-5	0.001124390620440529	0.0	0.511289863440082	2.141696419886722E-4
76-77	5.354241049716805E-5	0.0011779330309376972	0.0	0.514542564877785	2.186315095301029E-4
78-79	5.354241049716805E-5	0.001204704236186281	0.0	0.5172062998000191	2.2309337707153356E-4
80-81	5.354241049716805E-5	0.0012136279712691425	0.0	0.5186117880755697	2.2309337707153356E-4
82-83	5.354241049716805E-5	0.001222551706352004	0.0	0.519142750313	2.320171121543949E-4
84-85	5.354241049716805E-5	0.001222551706352004	0.0	0.5196692506828888	2.320171121543949E-4
86-87	5.354241049716805E-5	0.0012403991765177265	0.0	0.5199101915301261	2.320171121543949E-4
88-89	5.354241049716805E-5	0.0012939415870148947	0.0	0.5199681958081647	2.4094084723725624E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18215	0.0	55.952904	1
GTATCAA	33380	0.0	37.78699	1
ATCAACG	38360	0.0	32.3854	3
TCAACGC	38445	0.0	32.279766	4
CAACGCA	39270	0.0	31.568445	5
TATCAAC	40100	0.0	31.001713	2
AACGCAG	40250	0.0	30.741478	6
ACGCAGA	44950	0.0	27.517517	7
GTGGTAT	7630	0.0	27.259178	1
CGCAGAG	45435	0.0	27.224264	8
TGGTATC	7305	0.0	26.281792	2
GCAGAGT	52295	0.0	23.570372	9
GAGTACT	32925	0.0	22.590828	12-13
GTACATG	34850	0.0	21.700975	1
CAGAGTA	51765	0.0	21.519863	10-11
TACATGG	34760	0.0	21.147614	2
ACATGGG	34270	0.0	20.743399	3
GTACTTT	36720	0.0	20.197584	14-15
AGAGTAC	48315	0.0	18.865644	10-11
CATGGGG	24745	0.0	18.365536	4
>>END_MODULE