FastQCFastQC Report
Fri 27 May 2016
SRR2140395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14920268
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203450.053.089181
GTATCAA349900.033.6478651
TCAACGC415150.028.631494
ATCAACG418700.028.4521733
CAACGCA428200.027.7383545
TATCAAC439700.027.004052
AACGCAG441850.026.9850186
ACGCAGA512550.023.1692397
GTGGTAT98700.023.1500451
TGGTATC93600.023.0138242
CGCAGAG517650.022.949628
GAGTACT363400.020.17697312-13
GCAGAGT613450.019.3159439
CAGAGTA605550.018.85905810-11
GTACTTT402050.018.17258314-15
AGAGTAC553450.017.16579610-11
GTACATG401000.016.9527231
TACATGG404400.016.7670612
ACATGGG407250.016.307093
AGTACTT395950.015.46950312-13
Produced by FastQC (version 0.11.5)