##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140395_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14920268 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.485015617681935 30.0 18.0 32.0 18.0 33.0 2 26.6614798742221 28.0 18.0 31.0 18.0 33.0 3 29.427496007444372 31.0 28.0 33.0 25.0 33.0 4 30.14118687412317 32.0 29.0 33.0 25.0 33.0 5 31.34208487407867 33.0 32.0 33.0 28.0 33.0 6 34.97822043142925 37.0 34.0 38.0 29.0 38.0 7 35.28651898209871 38.0 35.0 38.0 29.0 38.0 8 35.89790216904951 38.0 36.0 38.0 31.0 38.0 9 36.127705883031055 38.0 37.0 38.0 33.0 38.0 10-11 36.23074367699025 38.0 37.0 38.0 33.0 38.0 12-13 36.240804689299146 38.0 37.0 38.0 33.0 38.0 14-15 36.35219347266416 38.0 37.0 38.0 33.0 38.0 16-17 36.372347768820234 38.0 37.0 38.0 33.0 38.0 18-19 36.40023382958 38.0 37.0 38.0 33.0 38.0 20-21 36.41443619970461 38.0 37.0 38.0 33.5 38.0 22-23 36.42757048983007 38.0 38.0 38.0 34.0 38.0 24-25 36.43555289372985 38.0 38.0 38.0 34.0 38.0 26-27 36.380360877601646 38.0 37.5 38.0 33.0 38.0 28-29 36.34937008928689 38.0 37.0 38.0 33.0 38.0 30-31 36.342045393353786 38.0 37.0 38.0 33.0 38.0 32-33 36.3171738518691 38.0 37.0 38.0 33.0 38.0 34-35 36.291593354390486 38.0 37.0 38.0 33.0 38.0 36-37 36.27170119688148 38.0 37.0 38.0 33.0 38.0 38-39 36.25406246489177 38.0 37.0 38.0 33.0 38.0 40-41 36.24955902002423 38.0 37.0 38.0 33.0 38.0 42-43 36.208367405622326 38.0 37.0 38.0 33.0 38.0 44-45 36.16319697532224 38.0 37.0 38.0 33.0 38.0 46-47 36.12498132426688 38.0 37.0 38.0 33.0 38.0 48-49 36.0735972256693 38.0 37.0 38.0 32.5 38.0 50-51 36.03708852986111 38.0 37.0 38.0 31.0 38.0 52-53 36.01836126482367 38.0 37.0 38.0 31.0 38.0 54-55 35.98849342007962 38.0 37.0 38.0 31.0 38.0 56-57 35.96296122020783 38.0 37.0 38.0 31.0 38.0 58-59 35.9359413027031 38.0 37.0 38.0 31.0 38.0 60-61 35.935431179189 38.0 37.0 38.0 31.0 38.0 62-63 35.93326784393095 38.0 37.0 38.0 31.0 38.0 64-65 35.905799518399206 38.0 37.0 38.0 31.0 38.0 66-67 35.88861238607286 38.0 37.0 38.0 31.0 38.0 68-69 35.88171038765512 38.0 37.0 38.0 31.0 38.0 70-71 35.84967685920469 38.0 37.0 38.0 31.0 38.0 72-73 35.83901563897675 38.0 37.0 38.0 31.0 38.0 74-75 35.857717930378676 38.0 37.0 38.0 31.0 38.0 76-77 35.820080633969525 38.0 37.0 38.0 31.0 38.0 78-79 35.821354082385795 38.0 37.0 38.0 31.0 38.0 80-81 35.794826308574585 38.0 37.0 38.0 31.0 38.0 82-83 35.776649990341596 38.0 37.0 38.0 31.0 38.0 84-85 35.75239853679672 38.0 37.0 38.0 31.0 38.0 86-87 35.72735914501328 38.0 37.0 38.0 31.0 38.0 88-89 35.65093216584009 38.0 36.5 38.0 30.0 38.0 90-91 35.669381277135365 38.0 36.0 38.0 29.0 38.0 92-93 35.70351842162937 38.0 37.0 38.0 31.0 38.0 94-95 35.678551209244226 38.0 36.0 38.0 30.5 38.0 96-97 35.68098018442037 38.0 36.0 38.0 31.0 38.0 98-99 35.70624515573596 38.0 36.0 38.0 31.0 38.0 100-101 35.133997691481405 38.0 35.0 38.0 28.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 7.0 18 36.0 19 94.0 20 275.0 21 1096.0 22 4578.0 23 13825.0 24 32153.0 25 61275.0 26 100725.0 27 151181.0 28 210816.0 29 280557.0 30 360718.0 31 453141.0 32 569996.0 33 729792.0 34 985166.0 35 1481920.0 36 3011310.0 37 6471607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.511064278470066 24.128668466276878 14.10047728365201 23.259789971601048 2 15.939983911814979 21.688038269559 40.63583718949783 21.736140629128194 3 18.139882261968094 27.752266076075223 29.597386226683813 24.51046543527287 4 13.340232733787898 19.391832662126358 38.40214737974591 28.86578722433983 5 13.791866574958142 37.51202601475021 35.93826854759003 12.75783886270162 6 29.31820661666399 39.093225403189805 18.55837308016183 13.030194899984371 7 25.892872701750395 33.46230778160285 23.112661247103606 17.532158269543146 8 23.822568066471728 37.81212240959747 20.89938330866443 17.465926215266375 9 24.77147488626525 17.619285263603636 21.468197365699567 36.14104248443155 10-11 23.630128493670487 27.52730379910066 28.986774902434732 19.855792804794124 12-13 24.574500730265108 24.891244402370425 29.271182924246027 21.26307194311844 14-15 21.733546672686245 26.448307945387338 26.808272862757104 25.009872519169306 16-17 21.186101786077828 29.704765583045205 27.56754447182146 21.541588159055514 18-19 21.194760040503294 28.68146537314209 29.412920062829972 20.710854523524645 20-21 21.9389231857343 27.724895119682614 29.466485634282748 20.86969606030034 22-23 21.7288752585159 27.62740799749586 29.343112392375108 21.300604351613128 24-25 21.50138589255593 27.813125261701405 29.376632067421088 21.308856778321577 26-27 21.47772271062766 27.928554023763812 29.31206072045809 21.28166254515044 28-29 21.40678506102044 27.87994918895105 29.369265150184233 21.34400059984428 30-31 21.677908960527116 27.771049544146837 29.295294561098796 21.255746934227254 32-33 21.481219481102084 27.908345409107877 29.258919263899514 21.351515845890525 34-35 21.611437305832766 27.837600080250542 29.270656502721515 21.280306111195184 36-37 21.52586580625948 27.856036605805084 29.237751433810992 21.380346154124446 38-39 21.39653874398646 27.85810290328759 29.37216244014747 21.37319591257848 40-41 21.64051398376354 27.736854106447574 29.25737073521751 21.36526117457138 42-43 21.57310569535536 27.826923652939378 29.091967745454657 21.50800290625061 44-45 21.58161576003429 27.957185832650524 28.931380002361813 21.529818404953375 46-47 21.710203295462318 27.928952024554217 28.69806771088223 21.662776969101238 48-49 21.638063875839983 27.989425733515027 28.716519803415085 21.655990587229905 50-51 21.586788495381843 28.07520411911567 28.660352715936334 21.677654669566152 52-53 21.746817561549495 28.034371666330816 28.576328803606433 21.64248196851326 54-55 21.704148788033446 27.95452111039129 28.596186473929702 21.745143627645565 56-57 21.689976656803637 28.039249687245988 28.58187785280063 21.688895803149745 58-59 21.677318050584933 28.109610748641995 28.60615064469017 21.6069205560829 60-61 21.745233141405187 28.096821422068608 28.59446608641063 21.563479350115575 62-63 21.654577756432154 28.206765018366013 28.556014240354425 21.582642984847407 64-65 21.626913364352166 28.193619481002298 28.505943999309103 21.67352315533644 66-67 21.650805926598427 28.283769104387975 28.34889331618457 21.716531652829033 68-69 21.607180123588833 28.20535121508317 28.408718611679777 21.778750049648217 70-71 21.652427122672744 27.96991265104251 28.478039876907925 21.899620349376818 72-73 21.6875705502716 28.008460701892794 28.399737867654306 21.904230880181295 74-75 21.608719249642387 28.091709635817914 28.379646151228606 21.919924963311097 76-77 21.83730631523196 27.968801325279387 28.343662745806792 21.850229613681872 78-79 21.757798773115596 28.055676375902504 28.233390453889328 21.953134397092573 80-81 21.761341857677877 28.087245969204268 28.23136050041336 21.920051672704492 82-83 21.75126151216015 28.08228829873326 28.29191510018353 21.87453508892306 84-85 21.852876932254727 28.141556250714554 28.195849399016303 21.80971741801441 86-87 21.794071635526436 28.117048200663387 28.265945024657647 21.822935139152534 88-89 21.80441989888364 28.17355635453913 28.223934026350523 21.79808972022671 90-91 21.821884472711886 28.22286959519055 28.222711365326358 21.73253456677121 92-93 21.883517605936845 28.16048434764805 28.202552496281264 21.75344555013384 94-95 21.76979551171766 28.21590853585791 28.25332904227841 21.760966910146024 96-97 21.81878324748451 28.167347092329276 28.273314140326487 21.74055551985973 98-99 21.943389942323947 28.760246521632443 28.398334546894432 20.898028989149175 100-101 22.51897808815522 31.26839358662823 25.85469039308434 20.357937932132213 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3567.0 1 3115.5 2 3436.5 3 4385.5 4 5100.5 5 6996.0 6 9482.5 7 13418.0 8 17789.5 9 20208.0 10 19463.0 11 17831.0 12 19179.0 13 23861.0 14 33732.5 15 45860.0 16 55377.0 17 61095.5 18 62517.0 19 60476.5 20 56979.0 21 54171.5 22 55239.5 23 62963.0 24 77257.0 25 98613.5 26 126532.0 27 158135.5 28 193987.5 29 233297.0 30 271697.5 31 310155.5 32 351009.5 33 391243.5 34 427292.5 35 459696.5 36 494297.0 37 525923.5 38 550220.5 39 569352.0 40 586010.5 41 602460.0 42 613106.5 43 618424.0 44 626241.0 45 630246.0 46 623698.5 47 612811.5 48 599384.5 49 581408.5 50 560029.0 51 535431.5 52 505488.5 53 473018.0 54 439875.5 55 403652.0 56 365794.5 57 327883.5 58 290104.0 59 248555.0 60 203190.5 61 162721.5 62 127632.5 63 96889.0 64 70993.0 65 51135.5 66 37378.5 67 28063.0 68 21080.0 69 15420.5 70 11197.0 71 7951.0 72 5556.5 73 3834.0 74 2535.0 75 1607.0 76 1046.5 77 668.5 78 410.0 79 241.5 80 161.0 81 116.5 82 86.5 83 61.5 84 40.5 85 23.0 86 13.0 87 9.0 88 7.0 89 5.0 90 4.0 91 3.0 92 1.5 93 1.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.3404584957857325E-5 3 0.003585726476226834 4 8.780003147396549E-4 5 0.05231139279803822 6 0.0 7 0.0 8 0.0 9 1.7425960445214522E-4 10-11 0.0 12-13 1.0053438718392995E-5 14-15 4.289467186514344E-4 16-17 3.6862608634107645E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1397274703785668E-4 28-29 0.0 30-31 4.363048310154472E-5 32-33 0.0 34-35 4.043525723950318E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 1.6831273660605883E-4 44-45 3.4579862451681123E-4 46-47 8.36380983665584E-5 48-49 0.001927830523119446 50-51 6.118063345084022E-4 52-53 0.005220777870279222 54-55 0.0011634938309230097 56-57 0.002929027607508283 58-59 0.008159785573890774 60-61 0.008639235007246912 62-63 0.007068235145207894 64-65 0.010732999370029271 66-67 0.004808148163590012 68-69 0.004242999841584624 70-71 0.004619332411798695 72-73 0.0072591951818884734 74-75 0.005319274573105987 76-77 0.0026547609668428934 78-79 1.5340161593262224E-4 80-81 2.3822495725648704E-4 82-83 0.0 84-85 0.0 86-87 8.325941339498666E-4 88-89 2.8848462119393478E-5 90-91 6.6623056324381E-5 92-93 8.416995816795164E-6 94-95 6.89264383862738E-4 96-97 3.6629386282820576E-4 98-99 8.44756966977472E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 175.0 20-21 549.0 22-23 1224.0 24-25 1761.0 26-27 4061.0 28-29 9235.0 30-31 23513.0 32-33 31372.0 34-35 44250.0 36-37 68448.0 38-39 77259.0 40-41 80626.0 42-43 91761.0 44-45 110071.0 46-47 111021.0 48-49 102887.0 50-51 92441.0 52-53 81832.0 54-55 86651.0 56-57 89297.0 58-59 99081.0 60-61 99980.0 62-63 96326.0 64-65 105349.0 66-67 113908.0 68-69 114491.0 70-71 117854.0 72-73 104577.0 74-75 106482.0 76-77 112760.0 78-79 117861.0 80-81 119180.0 82-83 112621.0 84-85 111901.0 86-87 116725.0 88-89 123329.0 90-91 127433.0 92-93 127307.0 94-95 138829.0 96-97 230367.0 98-99 1265706.0 100-101 1.0149767E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.94606968686095 #Duplication Level Percentage of deduplicated Percentage of total 1 87.86102700010227 71.99865841309551 2 8.178910951673252 13.404592136168933 3 2.095849037451325 5.152397738283802 4 0.805794638549629 2.641268144155473 5 0.3911649381938003 1.602721464214291 6 0.21671202127916217 1.0655219038633632 7 0.12741460299847462 0.7308788155505708 8 0.0852774615250235 0.5590522243878543 9 0.05758988900694562 0.4247338552039579 >10 0.17699212916792642 2.167958412514383 >50 0.002675458227738354 0.14501041652493707 >100 5.46823865765565E-4 0.07146065052044533 >500 1.5016842245553352E-5 0.009229284008480342 >1k 3.0031116510477577E-5 0.026516541508023996 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.702292478928663E-6 2 0.0 2.010687743678599E-5 0.0 6.702292478928663E-6 6.702292478928663E-6 3 0.0 2.010687743678599E-5 0.0 6.702292478928663E-6 6.702292478928663E-6 4 0.0 6.032063231035796E-5 0.0 1.3404584957857325E-5 6.702292478928663E-6 5 0.0 6.032063231035796E-5 0.0 1.3404584957857325E-5 6.702292478928663E-6 6 6.702292478928663E-6 6.032063231035796E-5 0.0 1.3404584957857325E-5 2.010687743678599E-5 7 6.702292478928663E-6 6.032063231035796E-5 0.0 2.010687743678599E-5 2.010687743678599E-5 8 6.702292478928663E-6 7.372521726821529E-5 0.0 2.680916991571465E-5 2.010687743678599E-5 9 6.702292478928663E-6 1.7425960445214522E-4 0.0 6.032063231035796E-5 2.680916991571465E-5 10-11 6.702292478928663E-6 1.7425960445214522E-4 0.0 7.372521726821529E-5 2.680916991571465E-5 12-13 6.702292478928663E-6 1.7425960445214522E-4 0.0 8.712980222607261E-5 2.680916991571465E-5 14-15 1.3404584957857325E-5 2.4463367548089617E-4 0.0 1.0388553342339427E-4 3.351146239464331E-5 16-17 2.010687743678599E-5 2.479848217203605E-4 0.0 1.1393897214178727E-4 4.021375487357198E-5 18-19 2.010687743678599E-5 2.7144284539661084E-4 0.0 1.5415272701535922E-4 4.356490111303631E-5 20-21 2.010687743678599E-5 3.183588927491115E-4 0.0 2.0106877436785987E-4 4.691604735250064E-5 22-23 3.016031615517898E-5 3.451680626648261E-4 0.0 2.144733593257172E-4 8.712980222607261E-5 24-25 4.691604735250064E-5 3.753283788200051E-4 0.0 2.4128252924143185E-4 1.0388553342339427E-4 26-27 5.36183398314293E-5 3.8873296377786245E-4 0.0 3.2171003898857583E-4 1.1729011838125159E-4 28-29 5.36183398314293E-5 3.8873296377786245E-4 0.0 6.702292478928662E-4 1.2064126462071592E-4 30-31 5.36183398314293E-5 4.356490111303631E-4 0.0 0.0017392448982819879 1.2399241086018026E-4 32-33 6.032063231035796E-5 4.4235130360929174E-4 0.0 0.004148719044456842 1.3404584957857324E-4 34-35 7.707636350767961E-5 5.026719359196497E-4 0.0 0.007540079038794745 1.4745043453643058E-4 36-37 8.712980222607261E-5 5.629925682300076E-4 0.0 0.012684088516372493 1.4745043453643058E-4 38-39 8.712980222607261E-5 5.76397153187865E-4 0.0 0.023008970080162096 1.5080158077589493E-4 40-41 8.712980222607261E-5 6.568246629350089E-4 0.0 0.05563237872134737 1.5415272701535925E-4 42-43 8.712980222607261E-5 6.668781016534019E-4 0.0 0.06597066487009483 1.6420616573375224E-4 44-45 9.383209470500127E-5 7.238475877242956E-4 0.0 0.08044426547834127 1.7425960445214522E-4 46-47 1.0388553342339427E-4 7.841682200346535E-4 0.0 0.09695201185394256 1.7761075069160954E-4 48-49 1.072366796628586E-4 7.841682200346535E-4 0.0 0.11232707080060492 1.809618969310739E-4 50-51 1.072366796628586E-4 8.645957297817975E-4 0.0 0.12720616010382657 1.809618969310739E-4 52-53 1.072366796628586E-4 8.712980222607262E-4 0.0 0.15304349761009656 2.0777106684678856E-4 54-55 1.1058782590232294E-4 8.712980222607262E-4 0.0 0.21743912374764313 2.3793138300196753E-4 56-57 1.1393897214178727E-4 8.914048996975122E-4 0.0 0.2897166458404098 2.4128252924143185E-4 58-59 1.1393897214178727E-4 8.914048996975122E-4 0.0 0.3699397356669465 2.4463367548089617E-4 60-61 1.1393897214178727E-4 9.014583384159052E-4 0.0 0.39164510985995693 2.479848217203605E-4 62-63 1.1393897214178727E-4 9.282675083316198E-4 0.0 0.40441632817855555 2.479848217203605E-4 64-65 1.1393897214178727E-4 9.986415793603706E-4 0.0 0.4132600031045019 2.479848217203605E-4 66-67 1.1393897214178727E-4 0.001012046164318228 0.0 0.41815602776035926 2.546871141992892E-4 68-69 1.2064126462071592E-4 0.0010455576267128714 0.0 0.4226197545513258 2.680916991571465E-4 70-71 1.2064126462071592E-4 0.0010589622116707288 0.0 0.42685560339800865 2.9490086907286116E-4 72-73 1.2064126462071592E-4 0.001062313357910193 0.0 0.43039776497312243 3.2171003898857583E-4 74-75 1.2064126462071592E-4 0.001102527112783765 0.0 0.4335880561930925 3.250611852280401E-4 76-77 1.2064126462071592E-4 0.0011494431601362658 0.0 0.43654376717630006 3.2841233146750447E-4 78-79 1.2064126462071592E-4 0.0011762523300519803 0.0 0.43920122614419527 3.2841233146750447E-4 80-81 1.2064126462071592E-4 0.0011863057687703732 0.0 0.4402936998182606 3.418169164253618E-4 82-83 1.2064126462071592E-4 0.0011963592074887663 0.0 0.44071594424443317 3.753283788200051E-4 84-85 1.2064126462071592E-4 0.0012064126462071592 0.0 0.44127558566642366 3.753283788200051E-4 86-87 1.2064126462071592E-4 0.0012332218161228738 0.0 0.44154702851182026 3.8873296377786245E-4 88-89 1.2064126462071592E-4 0.001293542448433232 0.0 0.44162745602156744 3.954352562567911E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20345 0.0 53.08918 1 GTATCAA 34990 0.0 33.647865 1 TCAACGC 41515 0.0 28.63149 4 ATCAACG 41870 0.0 28.452173 3 CAACGCA 42820 0.0 27.738354 5 TATCAAC 43970 0.0 27.00405 2 AACGCAG 44185 0.0 26.985018 6 ACGCAGA 51255 0.0 23.169239 7 GTGGTAT 9870 0.0 23.150045 1 TGGTATC 9360 0.0 23.013824 2 CGCAGAG 51765 0.0 22.94962 8 GAGTACT 36340 0.0 20.176973 12-13 GCAGAGT 61345 0.0 19.315943 9 CAGAGTA 60555 0.0 18.859058 10-11 GTACTTT 40205 0.0 18.172583 14-15 AGAGTAC 55345 0.0 17.165796 10-11 GTACATG 40100 0.0 16.952723 1 TACATGG 40440 0.0 16.767061 2 ACATGGG 40725 0.0 16.30709 3 AGTACTT 39595 0.0 15.469503 12-13 >>END_MODULE