FastQCFastQC Report
Fri 27 May 2016
SRR2140394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16699206
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222650.050.6564141
GTATCAA406850.037.6066361
ATCAACG501150.030.3665313
TCAACGC502200.030.3192444
CAACGCA513400.029.6573775
AACGCAG531000.028.699756
TATCAAC533700.028.6520922
ACGCAGA602000.025.2431837
CGCAGAG606800.025.0294238
TGGTATC103150.021.5669292
GTGGTAT108600.021.5187841
GCAGAGT710300.021.497799
AGAGTAC646150.019.74018310-11
GAGTACT444400.019.57013112-13
CAGAGTA702250.018.81486910-11
AGTACTT482950.017.8783912-13
GTACTTT494300.017.62531714-15
TACATGG453750.017.5166662
GTACATG461950.017.5143131
ACATGGG453350.017.0175933
Produced by FastQC (version 0.11.5)