##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16699206
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.04636537809043	30.0	18.0	32.0	18.0	33.0
2	30.178945813351845	32.0	29.0	33.0	25.0	33.0
3	30.936325056412862	33.0	31.0	33.0	27.0	33.0
4	31.09424675640267	33.0	31.0	33.0	27.0	34.0
5	31.505231985281217	33.0	32.0	33.0	28.0	34.0
6	34.55042102001736	37.0	34.0	38.0	28.0	38.0
7	35.27611402602016	38.0	35.0	38.0	29.0	38.0
8	35.425361421375364	38.0	36.0	38.0	29.0	38.0
9	36.06079229156165	38.0	37.0	38.0	31.0	38.0
10-11	36.1002463530302	38.0	37.0	38.0	32.0	38.0
12-13	36.13318920672037	38.0	37.0	38.0	33.0	38.0
14-15	36.19413183477107	38.0	37.0	38.0	33.0	38.0
16-17	36.21375222869878	38.0	37.0	38.0	33.0	38.0
18-19	36.27147856610667	38.0	37.0	38.0	33.0	38.0
20-21	36.30454686297669	38.0	37.0	38.0	33.0	38.0
22-23	36.32284899106567	38.0	37.0	38.0	33.0	38.0
24-25	36.31011623124503	38.0	37.0	38.0	33.0	38.0
26-27	36.26287098640217	38.0	37.0	38.0	33.0	38.0
28-29	36.24143674068588	38.0	37.0	38.0	33.0	38.0
30-31	36.23709150769377	38.0	37.0	38.0	33.0	38.0
32-33	36.21126951402611	38.0	37.0	38.0	33.0	38.0
34-35	36.17825146361496	38.0	37.0	38.0	33.0	38.0
36-37	36.15435177553874	38.0	37.0	38.0	33.0	38.0
38-39	36.13925896478223	38.0	37.0	38.0	33.0	38.0
40-41	36.13254624388162	38.0	37.0	38.0	33.0	38.0
42-43	36.10119843600373	38.0	37.0	38.0	33.0	38.0
44-45	36.051878663721425	38.0	37.0	38.0	32.0	38.0
46-47	36.008628241646235	38.0	37.0	38.0	31.0	38.0
48-49	35.955791544536794	38.0	37.0	38.0	31.0	38.0
50-51	35.92343641303121	38.0	37.0	38.0	31.0	38.0
52-53	35.89698766012327	38.0	37.0	38.0	31.0	38.0
54-55	35.86302114833184	38.0	37.0	38.0	31.0	38.0
56-57	35.82873912130623	38.0	37.0	38.0	31.0	38.0
58-59	35.802858642190486	38.0	37.0	38.0	31.0	38.0
60-61	35.80047123417947	38.0	37.0	38.0	31.0	38.0
62-63	35.794001617102836	38.0	37.0	38.0	31.0	38.0
64-65	35.758045585306206	38.0	37.0	38.0	30.5	38.0
66-67	35.73882922576256	38.0	37.0	38.0	29.5	38.0
68-69	35.73402704735582	38.0	37.0	38.0	29.5	38.0
70-71	35.70327541403506	38.0	37.0	38.0	29.0	38.0
72-73	35.69165136722965	38.0	37.0	38.0	29.0	38.0
74-75	35.70249698570693	38.0	37.0	38.0	29.0	38.0
76-77	35.66452549667319	38.0	36.5	38.0	29.0	38.0
78-79	35.666411396191805	38.0	36.0	38.0	29.0	38.0
80-81	35.64248654576012	38.0	36.0	38.0	29.0	38.0
82-83	35.62512266532446	38.0	36.0	38.0	29.0	38.0
84-85	35.59778321715172	38.0	36.0	38.0	29.0	38.0
86-87	35.56835701414502	38.0	36.0	38.0	29.0	38.0
88-89	35.49030441939168	38.0	36.0	38.0	29.0	38.0
90-91	35.51300335018692	38.0	36.0	38.0	29.0	38.0
92-93	35.550398320115406	38.0	36.0	38.0	29.0	38.0
94-95	35.52361633781731	38.0	36.0	38.0	29.0	38.0
96-97	35.52021282012991	38.0	36.0	38.0	29.0	38.0
98-99	35.54645973335941	38.0	36.0	38.0	29.0	38.0
100-101	34.96962475913321	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	26.0
19	47.0
20	282.0
21	1592.0
22	6842.0
23	19929.0
24	43883.0
25	80500.0
26	127685.0
27	185159.0
28	254481.0
29	333728.0
30	422638.0
31	526407.0
32	657253.0
33	834770.0
34	1109574.0
35	1625229.0
36	3096329.0
37	7372849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.67284432565237	20.31059440790179	16.718794893601526	23.297766372844315
2	14.351388922323613	22.656556245847856	41.3188387519742	21.673216079854335
3	17.687424241525964	26.854165914359307	31.77745092455805	23.68095891955668
4	12.202114612576532	19.250944964500956	39.195073489254476	29.351866933668042
5	13.295054350551547	37.50582593743595	36.12150111977229	13.07761859224022
6	28.590035957398214	39.52801109226391	18.91251596033967	12.969436989998206
7	25.211665752251932	33.57042843833413	23.65888533862029	17.55902047079364
8	23.61281728005511	37.58160118511024	21.37964523582738	17.42593629900727
9	24.74936636091304	17.40254832948334	21.734756357724258	36.11332895187936
10-11	23.417745131115815	27.500987172683537	29.357862882822094	19.723404813378554
12-13	24.335935492980923	24.985837650005635	29.542590228541403	21.13563662847204
14-15	21.477290219353236	26.46611732220189	27.26254273766775	24.794049720777128
16-17	20.902707288292035	29.623334362624128	27.96176679236829	21.512191556715546
18-19	20.880882001216104	28.638358614176028	29.925398848304525	20.555360536303343
20-21	21.71743153438697	27.715685001963465	29.8779598117889	20.688923651860666
22-23	21.4425797291408	27.622006985011694	29.772859258368477	21.16255402747903
24-25	21.223884415994036	27.811467072643214	29.83034311351415	21.134305397848593
26-27	21.201732262304112	27.97218366447006	29.738062151508792	21.088021921717036
28-29	21.128690599931343	27.99202465561912	29.76109085748586	21.118193886963677
30-31	21.462243466453174	27.834261005461176	29.666271986597277	21.037223541488377
32-33	21.24073700548279	27.96116514190677	29.614322974390088	21.18377487822035
34-35	21.357546275090826	27.917174489021473	29.65218779556647	21.07309144032123
36-37	21.28777428651825	27.94405033582772	29.65077274168505	21.117402635968986
38-39	21.161563528651474	27.957446055478773	29.74526280236945	21.135727613500304
40-41	21.435365452560163	27.81511170419664	29.596460228740412	21.153062614502787
42-43	21.35957665622951	27.90906864315086	29.442863953838668	21.288490746780965
44-45	21.35714696259568	28.059818694252332	29.274331280779737	21.30870306237225
46-47	21.479403152598543	27.996247410105333	28.992417785921898	21.531931651374222
48-49	21.39476554038871	28.113480873451486	29.010140341938616	21.48161324422119
50-51	21.35530068271427	28.191017892406773	28.933823764438372	21.51985766044059
52-53	21.516268227931903	28.15246013079566	28.85542922937363	21.475842411898803
54-55	21.487109357468192	28.06026853538488	28.84842767976946	21.604194427377465
56-57	21.524712109465376	28.18370440126481	28.76222454041021	21.5293589488596
58-59	21.419648244582365	28.24524705985644	28.890566398671446	21.444538296889746
60-61	21.535541465386455	28.219357545315894	28.842575858667974	21.40252513062968
62-63	21.44318423697094	28.387236093561235	28.760947965394678	21.408631704073148
64-65	21.37418554520627	28.365006593931653	28.716535478462575	21.544272382399505
66-67	21.431039949184996	28.418677868475058	28.590919739670497	21.559362442669457
68-69	21.43486528434452	28.359458610276363	28.610564166072987	21.59511193930613
70-71	21.43566907859015	28.105106364108284	28.706443530238307	21.752781027063257
72-73	21.47193356343245	28.10211194694501	28.673283615053112	21.75267087456943
74-75	21.396941146403776	28.219346843017668	28.61759924188309	21.766112768695468
76-77	21.58618511731695	28.151078280887802	28.524355139276043	21.7383814625192
78-79	21.584716121648952	28.19582444061728	28.414200256293633	21.805259181440135
80-81	21.54906083389344	28.232060310035003	28.435815613413908	21.783063242657658
82-83	21.572078109032848	28.199043768151473	28.497251242201934	21.73162688061375
84-85	21.70629104794832	28.216483088348753	28.45839218264382	21.618833681059105
86-87	21.585407870130773	28.278355368981234	28.464689522904603	21.671547237983393
88-89	21.63454761727837	28.316755554527372	28.401676392131687	21.647020436062576
90-91	21.668943659124782	28.32875625208988	28.455526456728307	21.546773632057032
92-93	21.719829565130855	28.299494484108152	28.42256263935761	21.558113311403385
94-95	21.591724822326267	28.3575106953072	28.443271815274695	21.607492667091837
96-97	21.580921221527202	28.337205966706975	28.503446225286012	21.578426586479814
98-99	21.695672149185917	28.943949179486054	28.61798852671266	20.742390144615367
100-101	22.294282321915535	31.41984625089916	26.08915286990384	20.196718557281464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4047.0
1	3790.0
2	4462.5
3	5749.5
4	7002.0
5	9842.5
6	13007.5
7	18123.5
8	23427.5
9	25368.5
10	24212.5
11	22431.5
12	23973.0
13	29619.0
14	42531.5
15	59696.5
16	73003.5
17	79997.5
18	81080.5
19	77592.0
20	72789.0
21	69530.5
22	71139.5
23	80566.5
24	98274.0
25	125837.0
26	161598.0
27	200294.0
28	242985.0
29	289260.0
30	332583.0
31	375166.0
32	419566.0
33	462896.0
34	501664.0
35	535449.0
36	572043.0
37	606169.0
38	629431.0
39	646232.0
40	665123.5
41	681373.5
42	688432.0
43	695151.0
44	705455.5
45	707304.5
46	694573.0
47	678150.5
48	659376.5
49	635029.0
50	609253.0
51	580357.5
52	546302.5
53	510924.5
54	476280.5
55	437993.5
56	396601.0
57	355166.5
58	313671.5
59	268832.5
60	220333.5
61	175830.5
62	136393.5
63	102847.0
64	75987.0
65	55302.0
66	40286.0
67	30190.0
68	22801.0
69	16502.0
70	11865.5
71	8492.0
72	5868.5
73	3946.5
74	2657.0
75	1735.0
76	1110.5
77	694.0
78	428.0
79	266.0
80	173.0
81	111.5
82	66.5
83	40.5
84	27.5
85	22.5
86	20.0
87	21.0
88	18.0
89	11.0
90	7.5
91	6.5
92	4.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0034672307174365056
4	9.940592385051122E-4
5	0.0524515956028089
6	0.0
7	0.0
8	0.0
9	1.8563756863649686E-4
10-11	0.0
12-13	0.0
14-15	5.419419342452569E-4
16-17	3.2935697661313956E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.4676918344365734E-4
28-29	0.0
30-31	1.4996960566002089E-5
32-33	0.0
34-35	3.314094312857493E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.4774913883029285E-4
44-45	2.8226293232600413E-4
46-47	1.1889924828140523E-4
48-49	0.002268806130863223
50-51	7.12417866409628E-4
52-53	0.005633864528714901
54-55	0.0012703346235505208
56-57	0.0032049500274580114
58-59	0.008893420095695033
60-61	0.009183044690070098
62-63	0.00767848973480657
64-65	0.011524813217764378
66-67	0.005095713700013949
68-69	0.004673656554180277
70-71	0.005117061047021033
72-73	0.00773614683972657
74-75	0.005821437914136285
76-77	0.0028656581583337134
78-79	1.7194634485952968E-4
80-81	2.8958370315585423E-4
82-83	0.0
84-85	0.0
86-87	0.0010004819112310478
88-89	7.547847408640949E-6
90-91	9.162170999323717E-5
92-93	7.72652848697651E-6
94-95	7.430228783001263E-4
96-97	3.569417374710178E-4
98-99	6.554521265755072E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	236.0
20-21	881.0
22-23	1686.0
24-25	2587.0
26-27	5259.0
28-29	11490.0
30-31	30060.0
32-33	39276.0
34-35	54053.0
36-37	84061.0
38-39	96744.0
40-41	101644.0
42-43	117546.0
44-45	138660.0
46-47	137227.0
48-49	127461.0
50-51	114470.0
52-53	101510.0
54-55	108179.0
56-57	109115.0
58-59	122434.0
60-61	122924.0
62-63	120140.0
64-65	130501.0
66-67	140295.0
68-69	140863.0
70-71	146327.0
72-73	129468.0
74-75	131751.0
76-77	138720.0
78-79	143996.0
80-81	145602.0
82-83	138145.0
84-85	137065.0
86-87	143042.0
88-89	150129.0
90-91	154810.0
92-93	155422.0
94-95	167704.0
96-97	265108.0
98-99	1384905.0
100-101	1.100771E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.90744450216394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.54968729844042	70.83421466280375
2	8.124981899963931	13.147430443048366
3	2.218259233239107	5.384210574141172
4	0.9134225853136578	2.956107485131515
5	0.4281153449273423	1.731885925511686
6	0.25127658564579197	1.2198087844698093
7	0.14949125145772016	0.8466468591612168
8	0.09467473595702837	0.6127912756160238
9	0.061515148667593854	0.4479330129179142
>10	0.20431278035982062	2.4540933720991527
>50	0.0033902496940106113	0.18564590523393762
>100	7.879387421826533E-4	0.10535241930281147
>500	4.24931042351069E-5	0.022069783398822006
>1k	4.245448726805897E-5	0.05180949716384071
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1976617331386893E-5	0.0	0.0	0.0
3	0.0	2.3953234662773787E-5	0.0	0.0	0.0
4	0.0	7.78480126540148E-5	0.0	0.0	0.0
5	0.0	8.383632131970826E-5	0.0	0.0	0.0
6	6.587139532262791E-5	8.383632131970826E-5	0.0	0.0	5.988308665693447E-6
7	6.587139532262791E-5	8.98246299854017E-5	0.0	2.9941543328467233E-5	5.988308665693447E-6
8	6.587139532262791E-5	1.1976617331386893E-4	0.0	2.9941543328467233E-5	5.988308665693447E-6
9	6.587139532262791E-5	2.2755572929635098E-4	0.0	5.9883086656934465E-5	1.796492599708034E-5
10-11	6.886554965547463E-5	2.2755572929635098E-4	0.0	6.287724098978119E-5	1.796492599708034E-5
12-13	7.185970398832136E-5	2.2755572929635098E-4	0.0	7.185970398832135E-5	1.796492599708034E-5
14-15	7.185970398832136E-5	2.6647973562335836E-4	0.0	8.383632131970826E-5	2.694738899562051E-5
16-17	1.2575448197956238E-4	2.694738899562051E-4	0.0	8.383632131970826E-5	3.592985199416068E-5
18-19	1.2575448197956238E-4	3.1738035928175265E-4	0.0	8.98246299854017E-5	3.592985199416068E-5
20-21	1.2575448197956238E-4	3.7726344593868715E-4	0.0	1.0479540164963532E-4	3.592985199416068E-5
22-23	1.3174279064525582E-4	4.2816406959708144E-4	0.0	1.287486363124091E-4	6.287724098978119E-5
24-25	1.3773109931094926E-4	4.910413105868627E-4	0.0	1.8863172296934355E-4	7.78480126540148E-5
26-27	1.3773109931094926E-4	5.149945452496364E-4	0.0	2.604914269576649E-4	8.383632131970826E-5
28-29	1.4371940797664273E-4	5.179886995824831E-4	0.0	6.976379595532865E-4	8.383632131970826E-5
30-31	1.4371940797664273E-4	5.988308665693447E-4	0.0	0.0021468086566511007	8.98246299854017E-5
32-33	1.4371940797664273E-4	5.988308665693447E-4	0.0	0.005203840230487605	9.581293865109515E-5
34-35	1.4671356230948945E-4	6.916496508875931E-4	0.0	0.009198042110505135	1.0778955598248204E-4
36-37	1.4970771664233617E-4	7.844684352058415E-4	0.0	0.01549774282681464	1.1377786464817549E-4
38-39	1.4970771664233617E-4	8.024333612029219E-4	0.0	0.028037261172776717	1.1677201898102221E-4
40-41	1.556960253080296E-4	8.742930651912432E-4	0.0	0.0650180613377666	1.1976617331386893E-4
42-43	1.556960253080296E-4	9.012404541868637E-4	0.0	0.07727912333077393	1.1976617331386893E-4
44-45	1.556960253080296E-4	0.001018012473167886	0.0	0.09536680965550098	1.2276032764671566E-4
46-47	1.556960253080296E-4	0.001125802029150368	0.0	0.11545758522890251	1.3174279064525582E-4
48-49	1.5869017964087635E-4	0.001125802029150368	0.0	0.13312908410136387	1.6168433397372307E-4
50-51	1.7665510563795667E-4	0.0012425740481313901	0.0	0.1505430857012004	1.6168433397372307E-4
52-53	1.8563756863649686E-4	0.0012575448197956237	0.0	0.18179906278178734	1.8563756863649686E-4
54-55	1.8563756863649686E-4	0.0012635331284613172	0.0	0.2578296237557642	2.185732662978108E-4
56-57	1.8563756863649686E-4	0.0012695214371270106	0.0	0.3424504135106783	2.2755572929635098E-4
58-59	1.8563756863649686E-4	0.0012755097457927043	0.0	0.43494582916097924	2.3354403796204442E-4
60-61	1.9462003163503704E-4	0.0013084454434540182	0.0	0.46010870217422317	2.3953234662773786E-4
62-63	2.2755572929635098E-4	0.0013473694497810255	0.0	0.47510342707311953	2.4552065529343133E-4
64-65	2.2755572929635098E-4	0.0014701297774277412	0.0	0.4851488148598203	2.4552065529343133E-4
66-67	2.395323466277379E-4	0.0014910888577576683	0.0	0.49088262040722175	2.574972726248182E-4
68-69	2.4552065529343133E-4	0.001536001172750369	0.0	0.4962062268110232	2.724680442890518E-4
70-71	2.4552065529343133E-4	0.0015509719444146027	0.0	0.5016076812274788	3.083978962832125E-4
72-73	2.6348558129051165E-4	0.0015629485617459896	0.0	0.5058294388367925	3.592985199416068E-4
74-75	2.6348558129051165E-4	0.0015869017964087633	0.0	0.5095032662031955	3.592985199416068E-4
76-77	2.6348558129051165E-4	0.0016437907287328512	0.0	0.512560297777032	3.622926742744535E-4
78-79	2.6348558129051165E-4	0.0016767264263941651	0.0	0.5156442767398641	3.6528682860730027E-4
80-81	2.6348558129051165E-4	0.0016857088893927053	0.0	0.5168928390966612	3.68280982940147E-4
82-83	2.6348558129051165E-4	0.0016916971980583987	0.0	0.5174347810309066	4.072049892671544E-4
84-85	2.6647973562335836E-4	0.0017066679697226323	0.0	0.5180725359038029	4.072049892671544E-4
86-87	2.694738899562051E-4	0.0017575685933810265	0.0	0.5182881150157679	4.1319329793284784E-4
88-89	2.754621986218985E-4	0.0018354166060350415	0.0	0.5183420097937591	4.1319329793284784E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22265	0.0	50.656414	1
GTATCAA	40685	0.0	37.606636	1
ATCAACG	50115	0.0	30.366531	3
TCAACGC	50220	0.0	30.319244	4
CAACGCA	51340	0.0	29.657377	5
AACGCAG	53100	0.0	28.69975	6
TATCAAC	53370	0.0	28.652092	2
ACGCAGA	60200	0.0	25.243183	7
CGCAGAG	60680	0.0	25.029423	8
TGGTATC	10315	0.0	21.566929	2
GTGGTAT	10860	0.0	21.518784	1
GCAGAGT	71030	0.0	21.49779	9
AGAGTAC	64615	0.0	19.740183	10-11
GAGTACT	44440	0.0	19.570131	12-13
CAGAGTA	70225	0.0	18.814869	10-11
AGTACTT	48295	0.0	17.87839	12-13
GTACTTT	49430	0.0	17.625317	14-15
TACATGG	45375	0.0	17.516666	2
GTACATG	46195	0.0	17.514313	1
ACATGGG	45335	0.0	17.017593	3
>>END_MODULE