FastQCFastQC Report
Fri 27 May 2016
SRR2140393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12639171
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186300.052.159921
GTATCAA344750.037.4277341
TCAACGC403600.031.6694054
ATCAACG401700.031.5727023
CAACGCA414650.030.742055
TATCAAC424300.030.1457022
AACGCAG427750.029.8372766
TGGTATC83200.027.3804632
GTGGTAT87700.027.05991
ACGCAGA483600.026.3735587
CGCAGAG488450.026.1032548
GCAGAGT562850.022.6835169
GAGTACT351850.021.38510912-13
CAGAGTA551200.020.23524510-11
GTACTTT396250.018.91290914-15
AGAGTAC514100.018.77925310-11
AGTACTT384350.017.5140312-13
TATACCG10300.017.2062575
TACATGG347500.016.9797532
GTACATG358000.016.904351
Produced by FastQC (version 0.11.5)