FastQCFastQC Report
Fri 27 May 2016
SRR2140392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13271738
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185850.049.987511
GTATCAA318100.032.935861
ATCAACG411400.026.4665783
TCAACGC414450.026.3024624
TGGTATC90050.026.2125682
CAACGCA424050.025.6862875
TATCAAC430650.025.4243222
GTGGTAT92850.025.3754521
AACGCAG438100.024.950946
ACGCAGA496650.022.000767
CGCAGAG503650.021.6606838
AGAGTAC534350.018.64599610-11
GCAGAGT588250.018.560249
GAGTACT375500.018.46364412-13
CAGAGTA578200.017.74478310-11
AGTACTT407100.016.95601712-13
GTACTTT416500.016.74030314-15
TACATGG349300.016.081932
GTACATG359350.015.7406381
ACATGGG347650.015.5104343
Produced by FastQC (version 0.11.5)