FastQCFastQC Report
Fri 27 May 2016
SRR2140391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12980459
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189750.053.258861
GTATCAA351000.037.910451
ATCAACG423950.031.3565043
TCAACGC425500.031.2517644
CAACGCA435000.030.5589185
TATCAAC439450.030.3986382
AACGCAG450200.029.5594466
TGGTATC83050.027.610332
GTGGTAT89950.027.3270381
ACGCAGA512550.025.9636387
CGCAGAG518300.025.6506718
GCAGAGT596950.022.2565759
GAGTACT353350.021.01120612-13
GTACATG418150.020.8549371
TACATGG415000.020.5318552
ACATGGG415450.020.0706483
CAGAGTA583600.019.96922110-11
GAGACCG133550.019.71762788-89
GACCGAG137050.019.5736890-91
CGAGACC133250.019.15492288-89
Produced by FastQC (version 0.11.5)