##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12980459
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.81587130316424	31.0	18.0	33.0	18.0	33.0
2	29.792779361654315	31.0	29.0	33.0	18.0	33.0
3	30.78563978361628	33.0	30.0	33.0	27.0	33.0
4	30.388750736780572	33.0	31.0	33.0	25.0	33.0
5	31.347708582570153	33.0	32.0	33.0	28.0	34.0
6	34.66048712144925	37.0	34.0	38.0	28.0	38.0
7	35.09009612063795	38.0	35.0	38.0	29.0	38.0
8	35.585610108240395	38.0	36.0	38.0	29.0	38.0
9	36.05965906136293	38.0	37.0	38.0	31.0	38.0
10-11	36.15381193376906	38.0	37.0	38.0	33.0	38.0
12-13	36.17584150914848	38.0	37.0	38.0	33.0	38.0
14-15	36.24921526272684	38.0	37.0	38.0	33.0	38.0
16-17	36.24494434287725	38.0	37.0	38.0	33.0	38.0
18-19	36.27309823943822	38.0	37.0	38.0	33.0	38.0
20-21	36.289202730332036	38.0	37.0	38.0	33.0	38.0
22-23	36.286026349357535	38.0	37.0	38.0	33.0	38.0
24-25	36.270520791578384	38.0	37.0	38.0	33.0	38.0
26-27	36.21117384874644	38.0	37.0	38.0	33.0	38.0
28-29	36.1875590059736	38.0	37.0	38.0	33.0	38.0
30-31	36.18413337939336	38.0	37.0	38.0	33.0	38.0
32-33	36.156577074893974	38.0	37.0	38.0	33.0	38.0
34-35	36.123525954453896	38.0	37.0	38.0	33.0	38.0
36-37	36.0952847847783	38.0	37.0	38.0	33.0	38.0
38-39	36.0840123538	38.0	37.0	38.0	33.0	38.0
40-41	36.0763537109198	38.0	37.0	38.0	32.5	38.0
42-43	36.03125367345964	38.0	37.0	38.0	31.0	38.0
44-45	35.97573543609586	38.0	37.0	38.0	31.0	38.0
46-47	35.930703572306356	38.0	37.0	38.0	31.0	38.0
48-49	35.87269658443729	38.0	37.0	38.0	31.0	38.0
50-51	35.8373785065188	38.0	37.0	38.0	31.0	38.0
52-53	35.80978066321738	38.0	37.0	38.0	31.0	38.0
54-55	35.76968178876845	38.0	37.0	38.0	31.0	38.0
56-57	35.73483383765445	38.0	37.0	38.0	29.5	38.0
58-59	35.70843656164831	38.0	37.0	38.0	29.0	38.0
60-61	35.70884830561829	38.0	37.0	38.0	29.0	38.0
62-63	35.7018928143319	38.0	37.0	38.0	29.0	38.0
64-65	35.66811823179299	38.0	36.5	38.0	29.0	38.0
66-67	35.64871570359739	38.0	36.0	38.0	29.0	38.0
68-69	35.641928484486996	38.0	36.0	38.0	29.0	38.0
70-71	35.612929549285724	38.0	36.0	38.0	29.0	38.0
72-73	35.59877759907701	38.0	36.0	38.0	29.0	38.0
74-75	35.61103966522002	38.0	36.0	38.0	29.0	38.0
76-77	35.56737505604122	38.0	36.0	38.0	29.0	38.0
78-79	35.57039252677548	38.0	36.0	38.0	29.0	38.0
80-81	35.54140852272013	38.0	36.0	38.0	29.0	38.0
82-83	35.514612738209664	38.0	36.0	38.0	29.0	38.0
84-85	35.490852147727914	38.0	36.0	38.0	29.0	38.0
86-87	35.46178252872758	38.0	36.0	38.0	29.0	38.0
88-89	35.38433807949626	38.0	36.0	38.0	29.0	38.0
90-91	35.39984302576876	38.0	36.0	38.0	29.0	38.0
92-93	35.440686822153005	38.0	36.0	38.0	29.0	38.0
94-95	35.40913967975362	38.0	36.0	38.0	29.0	38.0
96-97	35.400794540026	38.0	36.0	38.0	29.0	38.0
98-99	35.4306242207327	38.0	36.0	38.0	29.0	38.0
100-101	34.836830967927376	37.5	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	16.0
20	181.0
21	1195.0
22	5489.0
23	16102.0
24	35927.0
25	64636.0
26	103117.0
27	150854.0
28	205634.0
29	269248.0
30	340968.0
31	423248.0
32	525947.0
33	666185.0
34	889080.0
35	1302422.0
36	2467828.0
37	5512375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.86718805552253	19.379938721735495	14.373960119592072	26.3789131031499
2	13.892228310262372	20.90331320333125	43.72339221594552	21.481066270460854
3	17.846448689406866	27.776572936337097	29.678416220475146	24.698562153780895
4	12.047201837979307	20.439476864322987	38.27262794770739	29.240693349990316
5	14.235376912000937	37.5109224161587	35.229725313181206	13.023975358659158
6	28.712567097973963	39.09263147011981	19.075450259501608	13.119351172404611
7	25.72691766909013	33.578265606786324	23.09383666633052	17.600980057793024
8	23.585537306500488	37.55894918661967	21.11210397105372	17.74340953582612
9	24.816225285585663	17.789517158559683	21.65347206113572	35.740785494718935
10-11	23.413717496430596	27.628029178321047	28.910183376412192	20.04806994883617
12-13	24.515567940668966	25.07144971309949	29.222759320202723	21.19022302602882
14-15	21.662735482938544	26.505637461529496	27.073170092845373	24.758456962686587
16-17	20.910098892893416	29.79993193614324	27.7644682400447	21.525500930918653
18-19	20.897600770512046	28.7103329705059	29.80847210410664	20.583594154875417
20-21	21.79693273237478	27.690191917629313	29.87331735406488	20.639557995931018
22-23	21.524838946989966	27.578900459880696	29.760016000808054	21.136244592321283
24-25	21.28314501567049	27.759997115094333	29.78283302360152	21.17402484563366
26-27	21.197672720434753	27.921636386266137	29.734753478916108	21.145937414383003
28-29	21.105276726827924	27.87997137571867	29.810121283133835	21.204630614319573
30-31	21.382336840899864	27.814300889358947	29.72765649048994	21.075705779251255
32-33	21.233829614362687	27.898548848163546	29.686228060363824	21.181393477109946
34-35	21.35291266668397	27.90950724810618	29.69936737672292	21.03821270848693
36-37	21.310987638519823	27.859184223487325	29.662661462280354	21.167166675712497
38-39	21.16377754022783	27.95173797600749	29.72985183055431	21.15463265321037
40-41	21.385141274649662	27.826048005306113	29.644460222723907	21.14435049732032
42-43	21.295584833757975	27.90477153496522	29.46547774841946	21.33416588285734
44-45	21.336803065630082	28.007587308638016	29.33003486588028	21.325574759851623
46-47	21.417521701011584	28.008795040503	29.06905034321497	21.50463291527044
48-49	21.361210189184774	28.110772597358512	29.012095154682722	21.51592205877399
50-51	21.345251050228402	28.22044648603112	28.94474151479703	21.48956094894345
52-53	21.478342034479116	28.200086501506092	28.816558610971942	21.505012853042846
54-55	21.466089927581187	28.101485915075397	28.829847301323447	21.60257685601997
56-57	21.462598308847205	28.160047038257307	28.822466724180607	21.554887928714876
58-59	21.38534222398257	28.163561708283364	28.910975052768546	21.540121014965525
60-61	21.42027380941364	28.065040041661167	28.957684305637464	21.557001843287733
62-63	21.490348084174535	28.14874051735876	28.97198393209821	21.388927466368493
64-65	21.292517375909263	28.149402379289324	28.873682887001724	21.68439735779969
66-67	21.371216984098947	28.244848636283347	28.713384714138623	21.67054966547908
68-69	21.31734177057824	28.376007126706206	28.749712348868506	21.55693875384705
70-71	21.333389083235993	28.166184609894223	28.846463583460125	21.653962723409656
72-73	21.363699576688294	28.173789580844417	28.59852695194629	21.863983890521
74-75	21.30904340650598	28.24573698422657	28.555959214665773	21.889260394601674
76-77	21.516462009191766	28.00305605167532	28.640071423222334	21.840410515910584
78-79	21.48281997452843	28.028833932394985	28.534089194890623	21.954256898185964
80-81	21.683749947384452	28.089194429754983	28.336547247292344	21.890508375568224
82-83	21.689477083217	28.261183087367588	28.40708824358516	21.64225158583025
84-85	21.57168208421984	28.11043194489895	28.350363837256708	21.967522133624502
86-87	21.49305290457605	28.340687390432862	28.390571691891946	21.77568801309914
88-89	21.749219872077337	28.128995371055254	28.33582259702353	21.785962159843876
90-91	21.774792162956157	28.313098715196215	28.355179743689245	21.556929378158372
92-93	21.594720129338103	28.275942747027543	28.372719304829232	21.756617818805125
94-95	21.729856082392704	28.13253080346938	28.40728174405629	21.730331370081622
96-97	21.690433575709836	28.346081486201165	28.470908195232525	21.49257674285648
98-99	21.926820320457253	28.783142413834867	28.61313151394249	20.67690575176539
100-101	22.31734763949595	31.348106981155027	26.168210564445403	20.166334814903625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3158.0
1	2847.0
2	3471.5
3	4497.0
4	5273.5
5	7823.0
6	10666.5
7	14313.0
8	18789.5
9	20983.0
10	19544.0
11	17442.5
12	18372.5
13	22775.0
14	32316.0
15	44512.5
16	55080.0
17	62734.5
18	65154.5
19	61927.5
20	56598.0
21	52271.0
22	51793.5
23	57855.5
24	70642.0
25	90208.5
26	116320.0
27	145724.5
28	177482.5
29	212079.5
30	245261.0
31	277862.5
32	312385.5
33	346977.0
34	377916.0
35	406608.5
36	437757.0
37	463090.5
38	482387.0
39	499432.5
40	516427.0
41	531001.5
42	536180.5
43	540490.0
44	544443.0
45	543319.5
46	539369.5
47	532926.5
48	522649.0
49	507055.0
50	488767.5
51	465686.0
52	437332.0
53	407032.5
54	375232.0
55	342230.5
56	308416.0
57	274383.0
58	240329.5
59	204561.0
60	168258.0
61	134766.5
62	103508.0
63	77164.5
64	56843.0
65	41285.0
66	29899.0
67	21882.5
68	15941.5
69	11410.0
70	8166.5
71	5742.5
72	3813.5
73	2390.0
74	1485.5
75	934.0
76	595.0
77	353.0
78	225.0
79	153.0
80	104.5
81	76.0
82	54.5
83	37.0
84	25.5
85	18.5
86	15.5
87	13.5
88	10.5
89	8.5
90	7.0
91	4.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003235632884784737
4	9.013548750471766E-4
5	0.05499805515351961
6	0.0
7	0.0
8	0.0
9	2.1570885898564913E-4
10-11	0.0
12-13	7.70388782091604E-6
14-15	4.622332692549624E-4
16-17	1.155583173137406E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.2716095104523028E-4
28-29	0.0
30-31	3.0866040919419104E-5
32-33	0.0
34-35	2.324538416406654E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.264950829384776E-4
44-45	2.2695460171358688E-4
46-47	1.0038157042550142E-4
48-49	0.002234874997611275
50-51	6.690412463520424E-4
52-53	0.0056221585819971505
54-55	0.0012643640118865092
56-57	0.00327753005667673
58-59	0.008736749019670585
60-61	0.009127269041186697
62-63	0.007591980274585523
64-65	0.011388749496082009
66-67	0.005152175269076231
68-69	0.004637846961047519
70-71	0.005238344453262276
72-73	0.007654613225985168
74-75	0.005822998441410164
76-77	0.0028701477999956656
78-79	1.228267456421412E-4
80-81	2.711186867084339E-4
82-83	0.0
84-85	0.0
86-87	9.466520396304517E-4
88-89	1.9131622357271282E-5
90-91	6.770876910777737E-5
92-93	9.78529305680617E-6
94-95	7.772873661097757E-4
96-97	3.613352058526668E-4
98-99	4.154712347451946E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	176.0
20-21	660.0
22-23	1370.0
24-25	1844.0
26-27	3888.0
28-29	8276.0
30-31	21198.0
32-33	28443.0
34-35	39671.0
36-37	62262.0
38-39	69745.0
40-41	74102.0
42-43	86173.0
44-45	103343.0
46-47	104976.0
48-49	96194.0
50-51	85290.0
52-53	72741.0
54-55	78543.0
56-57	80602.0
58-59	49660.0
60-61	93604.0
62-63	89806.0
64-65	96065.0
66-67	102769.0
68-69	104947.0
70-71	105902.0
72-73	97055.0
74-75	98492.0
76-77	104447.0
78-79	109652.0
80-81	110536.0
82-83	104454.0
84-85	103732.0
86-87	107922.0
88-89	113841.0
90-91	119461.0
92-93	118912.0
94-95	127621.0
96-97	205951.0
98-99	1133545.0
100-101	8662588.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.12958808296732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.86974196599633	74.76574784638863
2	7.738740445022095	13.02114091841416
3	1.9246525057704813	4.857606675399644
4	0.673486531302716	2.266405778316958
5	0.3239726945951226	1.3627844673208318
6	0.1578088428415998	0.7965835766468109
7	0.0954255557031	0.5619678885723038
8	0.054214818121806486	0.36488562532644564
9	0.04048154591516881	0.30651262045243916
>10	0.11905308027789901	1.4806395199304303
>50	0.001997519121407698	0.11349978936369362
>100	3.7936196016252993E-4	0.05242063163663071
>500	0.0	0.0
>1k	4.513337220590499E-5	0.04980466223101049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.70388782091604E-6
2	0.0	1.540777564183208E-5	0.0	0.0	7.70388782091604E-6
3	0.0	1.540777564183208E-5	0.0	0.0	7.70388782091604E-6
4	0.0	3.85194391045802E-5	0.0	7.70388782091604E-6	7.70388782091604E-6
5	7.70388782091604E-6	3.85194391045802E-5	0.0	1.540777564183208E-5	7.70388782091604E-6
6	7.70388782091604E-6	3.85194391045802E-5	0.0	1.540777564183208E-5	7.70388782091604E-6
7	7.70388782091604E-6	3.85194391045802E-5	0.0	3.081555128366416E-5	7.70388782091604E-6
8	7.70388782091604E-6	4.622332692549624E-5	0.0	3.081555128366416E-5	1.540777564183208E-5
9	7.70388782091604E-6	1.2326220513465664E-4	0.0	3.85194391045802E-5	1.540777564183208E-5
10-11	3.85194391045802E-5	1.4637386859740477E-4	0.0	6.933499038824437E-5	1.540777564183208E-5
12-13	6.933499038824437E-5	1.579297003287788E-4	0.0	8.474276603007643E-5	1.540777564183208E-5
14-15	7.318693429870238E-5	2.2726469071702318E-4	0.0	9.62985977614505E-5	1.540777564183208E-5
16-17	8.474276603007644E-5	2.311166346274812E-4	0.0	1.2326220513465664E-4	2.311166346274812E-5
18-19	8.474276603007644E-5	2.3882052244839726E-4	0.0	1.540777564183208E-4	3.85194391045802E-5
20-21	8.474276603007644E-5	2.6578412982160337E-4	0.0	1.6948553206015286E-4	4.2371383015038215E-5
22-23	9.244665385099249E-5	2.888957932843515E-4	0.0	2.542282980902293E-4	5.3927214746412276E-5
24-25	1.3096609295557268E-4	3.4667495194122175E-4	0.0	3.081555128366416E-4	6.548304647778635E-5
26-27	1.4637386859740477E-4	3.697866154039699E-4	0.0	4.083060545085501E-4	6.933499038824437E-5
28-29	1.4637386859740477E-4	3.697866154039699E-4	0.0	7.70388782091604E-4	6.933499038824437E-5
30-31	1.694855320601529E-4	3.7749050322488593E-4	0.0	0.0021917560850506133	7.70388782091604E-5
32-33	1.7718941988106892E-4	3.8134244713534395E-4	0.0	0.0054235370259248915	8.474276603007644E-5
34-35	1.88745251612443E-4	4.237138301503822E-4	0.0	0.009695342822622837	9.244665385099248E-5
36-37	2.0030108334381704E-4	4.699371570758785E-4	0.0	0.01632453829252109	1.0015054167190852E-4
38-39	2.0030108334381704E-4	5.123085400909166E-4	0.0	0.029559817568854847	1.0015054167190852E-4
40-41	2.1570885898564913E-4	5.469760352850388E-4	0.0	0.07337182760640437	1.0015054167190852E-4
42-43	2.1570885898564913E-4	5.662357548373289E-4	0.0	0.08620265277214004	1.0785442949282455E-4
44-45	2.1956080289610715E-4	6.201629695837413E-4	0.0	0.10527747901672815	1.1941026122419863E-4
46-47	2.2341274680656516E-4	6.702382404196955E-4	0.0	0.12688303241048718	1.2326220513465664E-4
48-49	2.2341274680656516E-4	6.856460160615276E-4	0.0	0.1468476576983911	1.3866998077648873E-4
50-51	2.2341274680656516E-4	7.395732308079398E-4	0.0	0.16645020025871196	1.3866998077648873E-4
52-53	2.2341274680656516E-4	7.472771186288558E-4	0.0	0.20144125874131263	1.3866998077648873E-4
54-55	2.2341274680656516E-4	7.54981006449772E-4	0.0	0.2853520048867301	1.3866998077648873E-4
56-57	2.2341274680656516E-4	7.81944613822978E-4	0.0	0.38097266051994005	1.3866998077648873E-4
58-59	2.2341274680656516E-4	8.320198846589323E-4	0.0	0.4767281341900159	1.3866998077648873E-4
60-61	2.2341274680656516E-4	8.628354359425964E-4	0.0	0.8240579165960156	1.3866998077648873E-4
62-63	2.2341274680656516E-4	8.975029311367186E-4	0.0	0.8314844644553787	1.3866998077648873E-4
64-65	2.2341274680656516E-4	9.321704263308408E-4	0.0	0.8391498328371901	1.3866998077648873E-4
66-67	2.3882052244839723E-4	9.398743141517568E-4	0.0	0.8456673219336851	1.5022581250786278E-4
68-69	2.3882052244839723E-4	9.706898654354211E-4	0.0	0.8516185752753427	1.6178164423923683E-4
70-71	2.3882052244839723E-4	9.78393753256337E-4	0.0	0.8575852363926422	1.694855320601529E-4
72-73	2.3882052244839723E-4	9.86097641077253E-4	0.0	0.8618493383015192	1.92597195522901E-4
74-75	2.3882052244839723E-4	9.976534728086271E-4	0.0	0.8653661630917674	1.92597195522901E-4
76-77	2.3882052244839723E-4	0.0010092093045400014	0.0	0.8682974924076259	1.92597195522901E-4
78-79	2.3882052244839723E-4	0.0010169131923609173	0.0	0.8708975545471851	1.92597195522901E-4
80-81	2.3882052244839723E-4	0.0010169131923609173	0.0	0.872056989664233	1.92597195522901E-4
82-83	2.3882052244839723E-4	0.0010284690240922913	0.0	0.8725692982043238	1.92597195522901E-4
84-85	2.3882052244839723E-4	0.0010554326314654973	0.0	0.8731008664639671	2.0030108334381704E-4
86-87	2.3882052244839723E-4	0.0010708404071073296	0.0	0.8734167258646246	2.0030108334381704E-4
88-89	2.3882052244839723E-4	0.0011170637340328258	0.0	0.8735361361258489	2.0800497116473307E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18975	0.0	53.25886	1
GTATCAA	35100	0.0	37.91045	1
ATCAACG	42395	0.0	31.356504	3
TCAACGC	42550	0.0	31.251764	4
CAACGCA	43500	0.0	30.558918	5
TATCAAC	43945	0.0	30.398638	2
AACGCAG	45020	0.0	29.559446	6
TGGTATC	8305	0.0	27.61033	2
GTGGTAT	8995	0.0	27.327038	1
ACGCAGA	51255	0.0	25.963638	7
CGCAGAG	51830	0.0	25.650671	8
GCAGAGT	59695	0.0	22.256575	9
GAGTACT	35335	0.0	21.011206	12-13
GTACATG	41815	0.0	20.854937	1
TACATGG	41500	0.0	20.531855	2
ACATGGG	41545	0.0	20.070648	3
CAGAGTA	58360	0.0	19.969221	10-11
GAGACCG	13355	0.0	19.717627	88-89
GACCGAG	13705	0.0	19.57368	90-91
CGAGACC	13325	0.0	19.154922	88-89
>>END_MODULE