FastQCFastQC Report
Fri 27 May 2016
SRR2140390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9703805
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116450.042.780621
GTATCAA201600.035.150291
TCAACGC258400.028.1835384
ATCAACG258850.028.1182563
CAACGCA267000.027.2102035
TATCAAC272150.026.535552
AACGCAG277800.026.2500576
TGGTATC62150.023.2534712
GTGGTAT64450.023.1010861
ACGCAGA332450.021.8955447
CGCAGAG335100.021.6704128
GCAGAGT396500.018.380689
AGAGTAC360500.018.14019810-11
GAGACCG100950.017.7598188-89
GACCGAG103400.017.55561890-91
ACGAGAC104650.017.22895286-87
CGAGACC101950.017.15206388-89
AGACCGA102200.017.05018690-91
ACCGAGG106750.016.85355492-93
AGTACTT260850.016.73014312-13
Produced by FastQC (version 0.11.5)