##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140390_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9703805
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.164303281032545	28.0	18.0	32.0	18.0	33.0
2	26.946761914527343	29.0	25.0	32.0	18.0	33.0
3	28.53711167938762	30.0	27.0	33.0	18.0	33.0
4	29.297959099549093	31.0	29.0	33.0	15.0	33.0
5	30.7739032266209	33.0	32.0	33.0	27.0	33.0
6	33.7268011877815	36.0	33.0	38.0	26.0	38.0
7	35.26971626078636	37.0	35.0	38.0	29.0	38.0
8	35.815362015209494	38.0	36.0	38.0	31.0	38.0
9	36.017625251125715	38.0	37.0	38.0	31.0	38.0
10-11	36.1290796754469	38.0	37.0	38.0	33.0	38.0
12-13	36.12629272744042	38.0	37.0	38.0	32.5	38.0
14-15	36.2498853800133	38.0	37.0	38.0	33.0	38.0
16-17	36.267045401262706	38.0	37.0	38.0	33.0	38.0
18-19	36.3016014851906	38.0	37.0	38.0	33.0	38.0
20-21	36.319623679734065	38.0	37.0	38.0	33.0	38.0
22-23	36.3199739344577	38.0	37.0	38.0	33.0	38.0
24-25	36.3159630742258	38.0	37.0	38.0	33.0	38.0
26-27	36.25100023280273	38.0	37.0	38.0	33.0	38.0
28-29	36.221033603957075	38.0	37.0	38.0	33.0	38.0
30-31	36.21847466374787	38.0	37.0	38.0	33.0	38.0
32-33	36.19115940007049	38.0	37.0	38.0	33.0	38.0
34-35	36.158477850995666	38.0	37.0	38.0	33.0	38.0
36-37	36.13157051592317	38.0	37.0	38.0	33.0	38.0
38-39	36.11756570355738	38.0	37.0	38.0	33.0	38.0
40-41	36.111336685066064	38.0	37.0	38.0	33.0	38.0
42-43	36.072347927746854	38.0	37.0	38.0	32.5	38.0
44-45	36.01957198028728	38.0	37.0	38.0	31.0	38.0
46-47	35.98229456248951	38.0	37.0	38.0	31.0	38.0
48-49	35.92976460652355	38.0	37.0	38.0	31.0	38.0
50-51	35.894888655809766	38.0	37.0	38.0	31.0	38.0
52-53	35.872153051278175	38.0	37.0	38.0	31.0	38.0
54-55	35.83278936230354	38.0	37.0	38.0	31.0	38.0
56-57	35.79839949382652	38.0	37.0	38.0	31.0	38.0
58-59	35.76928779074687	38.0	37.0	38.0	31.0	38.0
60-61	35.76697766953818	38.0	37.0	38.0	31.0	38.0
62-63	35.762580597450295	38.0	37.0	38.0	30.5	38.0
64-65	35.73229697561666	38.0	37.0	38.0	29.5	38.0
66-67	35.71357009005405	38.0	37.0	38.0	29.0	38.0
68-69	35.7014174808921	38.0	37.0	38.0	29.0	38.0
70-71	35.6687718012696	38.0	36.5	38.0	29.0	38.0
72-73	35.657484163690256	38.0	36.0	38.0	29.0	38.0
74-75	35.676980571347805	38.0	36.0	38.0	29.0	38.0
76-77	35.63593990222971	38.0	36.0	38.0	29.0	38.0
78-79	35.637214752365416	38.0	36.0	38.0	29.0	38.0
80-81	35.60359757382255	38.0	36.0	38.0	29.0	38.0
82-83	35.57672377248918	38.0	36.0	38.0	29.0	38.0
84-85	35.558156422230574	38.0	36.0	38.0	29.0	38.0
86-87	35.530503453196445	38.0	36.0	38.0	29.0	38.0
88-89	35.453580792527674	38.0	36.0	38.0	29.0	38.0
90-91	35.465941432920815	38.0	36.0	38.0	29.0	38.0
92-93	35.504591981030785	38.0	36.0	38.0	29.0	38.0
94-95	35.47314095180278	38.0	36.0	38.0	29.0	38.0
96-97	35.46617437749488	38.0	36.0	38.0	29.0	38.0
98-99	35.49215357297636	38.0	36.0	38.0	29.0	38.0
100-101	34.887094515797294	38.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	18.0
19	83.0
20	249.0
21	983.0
22	3777.0
23	11466.0
24	25145.0
25	46355.0
26	75587.0
27	110409.0
28	152898.0
29	200130.0
30	254421.0
31	318301.0
32	397872.0
33	511277.0
34	690079.0
35	1042195.0
36	2108401.0
37	3754153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.966148845736285	25.216737145892772	14.09384257000218	23.723271438368766
2	13.408245528429314	25.77261187750578	38.940271367777896	21.878871226287007
3	19.42754229270812	27.5235367129026	29.655897565059604	23.393023429329677
4	13.805320222223823	19.616807145293613	38.56702525729796	28.010847375184593
5	13.800386647763888	39.547003479829876	34.04221162520943	12.610398247196802
6	28.856185795159732	39.834693710353825	18.584246076667863	12.724874417818576
7	25.365658110401025	33.67736676489274	23.316595912634273	17.640379212071966
8	23.449090331060855	38.241658813218116	21.058368341078577	17.250882514642452
9	24.39716576631371	17.818538267585634	21.764997521070896	36.01929844502976
10-11	23.370404702073053	27.99385395728789	29.08323075329729	19.55251058734177
12-13	24.220700024371883	25.21300665048401	29.478039799851707	21.088253525292398
14-15	21.289542470626976	26.861156235374544	27.140687070820334	24.70861422317814
16-17	20.832595220657304	30.01645849570524	27.867332014056757	21.2836142695807
18-19	20.832070512546366	29.078134814127036	29.748387359391497	20.3414073139351
20-21	21.54740969934408	28.148805637174885	29.71742193399882	20.586362729482218
22-23	21.23588399654397	28.13638738009748	29.611126452811664	21.016602170546886
24-25	21.07553810738148	28.22881306624489	29.64967123970052	21.04597758667311
26-27	21.09670546425515	28.32305742054779	29.559965721414645	21.020271393782412
28-29	21.024546991553674	28.318762848537943	29.642919569377078	21.01377059053131
30-31	21.295317383659896	28.17594336114982	29.603277972318924	20.92546128287136
32-33	21.150709199420007	28.247743282520805	29.595733664046154	21.00581385401304
34-35	21.174879723006477	28.318512502673283	29.59384962640358	20.912758147916662
36-37	21.198764118260527	28.22793897606494	29.558675819012848	21.014621086661688
38-39	21.0386951259345	28.328269703169745	29.641796070306793	20.991239100588963
40-41	21.26565267107572	28.23366191128369	29.51791093671574	20.98277448092485
42-43	21.229538379191386	28.287207724796037	29.323173115447105	21.160080780565476
44-45	21.228595820768177	28.405377681905552	29.16701179746566	21.199014699860612
46-47	21.33079330710697	28.33821628892268	28.958865150654812	21.372125253315545
48-49	21.247076303841375	28.441141936050084	28.999859772693586	21.31192198741495
50-51	21.237735310880105	28.51851175485047	28.949405721780547	21.29434721248888
52-53	21.35510870183452	28.4750733939736	28.848398239419677	21.321419664772208
54-55	21.337968043206445	28.41588151734075	28.893420025046527	21.35273041440627
56-57	21.347777599883027	28.498152203611404	28.812369181179125	21.341701015326443
58-59	21.30502467143058	28.54425607594038	28.87882936394643	21.271889888682615
60-61	21.316581423301972	28.415518183215916	28.970318284090695	21.297582109391417
62-63	21.352242430771103	28.553840655006457	28.919721954776662	21.174194959445774
64-65	21.157984810371993	28.507752451641284	28.90393797834368	21.43032475964304
66-67	21.19970761777884	28.580739695898004	28.728177956126533	21.491374730196622
68-69	21.17357226462818	28.71686081853933	28.759358137573027	21.35020877925946
70-71	21.181551397638327	28.507643021682043	28.814938089218963	21.495867491460665
72-73	21.25303799635875	28.478994693621285	28.6515030903179	21.616464219702074
74-75	21.174734186015563	28.5191789567106	28.62433360027999	21.68175325699385
76-77	21.33023523324494	28.323658287782898	28.704327953328622	21.64177852564353
78-79	21.321307102323264	28.316004165530373	28.62411775099191	21.73857098115445
80-81	21.473136625234858	28.388623214604447	28.444602832063886	21.693637328096813
82-83	21.512591040102492	28.55641252436029	28.501729064360287	21.429267371176923
84-85	21.44183247682941	28.391274106327764	28.46893382145651	21.697959595386315
86-87	21.338456924214512	28.580432964149953	28.509710199887333	21.571399911748195
88-89	21.61491656059182	28.432232144295057	28.416234867558277	21.53661642755485
90-91	21.61799027769889	28.614479553108563	28.46674320782328	21.300786961369266
92-93	21.456560740290403	28.535300307963997	28.45921417404737	21.54892477769823
94-95	21.50912472261399	28.46628615836802	28.496309805415194	21.5282793136028
96-97	21.489879278835957	28.66008290926711	28.579271255734977	21.270766556161952
98-99	21.72212652332758	29.063410853422756	28.754901176555613	20.459561446694046
100-101	22.10409926721412	31.677763670243074	26.29148963286876	19.92664742967404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1982.0
1	1805.0
2	1939.0
3	2517.5
4	3207.5
5	4457.5
6	6272.0
7	9609.5
8	12253.0
9	12799.0
10	12526.0
11	11985.0
12	12840.0
13	16307.5
14	24325.5
15	34375.0
16	41192.5
17	43392.5
18	42228.0
19	39682.5
20	37529.5
21	36722.0
22	38802.0
23	45120.5
24	55981.0
25	72569.5
26	93773.5
27	116471.5
28	141115.0
29	168443.0
30	194192.0
31	219502.5
32	245980.0
33	271327.0
34	294598.0
35	316125.5
36	338609.5
37	358437.5
38	372104.0
39	380900.5
40	390433.5
41	399901.5
42	403547.0
43	405041.0
44	406610.5
45	405948.0
46	402746.0
47	397700.5
48	388060.5
49	372196.5
50	353714.5
51	333841.0
52	311695.0
53	288012.0
54	263953.5
55	239458.0
56	215206.0
57	190860.5
58	165733.5
59	140221.5
60	114655.5
61	90925.0
62	69589.0
63	51770.5
64	37672.5
65	26724.5
66	19249.0
67	14358.5
68	10573.5
69	7519.0
70	5256.0
71	3618.5
72	2432.5
73	1582.5
74	1016.0
75	603.0
76	355.0
77	203.0
78	135.5
79	98.5
80	66.5
81	42.5
82	30.0
83	21.0
84	10.5
85	5.0
86	5.0
87	6.5
88	6.5
89	5.0
90	3.5
91	2.5
92	1.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0024835618605279063
4	7.728926951850331E-4
5	0.05209296765547122
6	0.0
7	0.0
8	0.0
9	1.8549424684440795E-4
10-11	0.0
12-13	0.0
14-15	4.688882350789201E-4
16-17	2.0610471871600883E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.5978504272832445E-4
28-29	0.0
30-31	4.644421538845478E-5
32-33	0.0
34-35	2.590389437593814E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.2145691690804909E-4
44-45	2.605088332963039E-4
46-47	8.036016784346095E-5
48-49	0.00197544958749806
50-51	6.413365540747808E-4
52-53	0.005348395464954392
54-55	9.955151218747013E-4
56-57	0.0027670384404174446
58-59	0.007826282159474564
60-61	0.008584469615116004
62-63	0.006968842906168615
64-65	0.010220571394067778
66-67	0.004439198003962671
68-69	0.004310132932115926
70-71	0.00465135909627411
72-73	0.0070461752303923155
74-75	0.005333518023413611
76-77	0.0026923875041699173
78-79	2.1084233198366128E-4
80-81	2.4325684425936654E-4
82-83	0.0
84-85	0.0
86-87	9.943237869703186E-4
88-89	4.420879891514133E-5
90-91	1.0211149988646477E-4
92-93	6.451804376129873E-6
94-95	7.567832900161887E-4
96-97	3.370445239781405E-4
98-99	3.415512382871834E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	126.0
20-21	469.0
22-23	822.0
24-25	1352.0
26-27	2696.0
28-29	5821.0
30-31	15197.0
32-33	20031.0
34-35	28154.0
36-37	43973.0
38-39	50126.0
40-41	52454.0
42-43	60610.0
44-45	70947.0
46-47	70496.0
48-49	65745.0
50-51	59321.0
52-53	51278.0
54-55	54860.0
56-57	56949.0
58-59	35063.0
60-61	66009.0
62-63	63986.0
64-65	68936.0
66-67	74171.0
68-69	75161.0
70-71	76444.0
72-73	68875.0
74-75	69969.0
76-77	74446.0
78-79	77751.0
80-81	78521.0
82-83	74844.0
84-85	73942.0
86-87	77046.0
88-89	81510.0
90-91	84984.0
92-93	84987.0
94-95	91875.0
96-97	150189.0
98-99	848938.0
100-101	6594731.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.41084727808625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10231346308863	75.21201773856944
2	7.5651314992996115	12.771583192520383
3	1.9007022881732265	4.81319671704298
4	0.6558399383480552	2.214400194990688
5	0.31076691718898597	1.311604939296059
6	0.16113657952752627	0.8161005123246733
7	0.0892498534743756	0.5273559025851966
8	0.05379327646776642	0.36325888356068015
9	0.03856913464407696	0.2930088000680214
>10	0.12024085770610121	1.4969259406899758
>50	0.001789649299636193	0.09781986117770095
>100	4.4686454817701165E-4	0.06762831457329586
>500	1.967823384434358E-5	0.015099002600892548
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0610471871600883E-5	0.0	0.0	0.0
3	0.0	2.0610471871600883E-5	0.0	0.0	0.0
4	0.0	5.152617967900221E-5	0.0	0.0	0.0
5	0.0	7.213665155060309E-5	0.0	0.0	0.0
6	0.0	7.213665155060309E-5	0.0	0.0	1.0305235935800442E-5
7	0.0	9.274712342220397E-5	0.0	0.0	1.0305235935800442E-5
8	0.0	9.274712342220397E-5	0.0	0.0	1.0305235935800442E-5
9	0.0	1.236628312296053E-4	0.0	3.0915707807401325E-5	1.0305235935800442E-5
10-11	4.1220943743201766E-5	1.236628312296053E-4	0.0	3.0915707807401325E-5	1.0305235935800442E-5
12-13	4.1220943743201766E-5	1.236628312296053E-4	0.0	3.0915707807401325E-5	1.0305235935800442E-5
14-15	4.1220943743201766E-5	2.009521007481086E-4	0.0	3.0915707807401325E-5	2.5763089839501104E-5
16-17	4.1220943743201766E-5	2.1125733668390906E-4	0.0	3.0915707807401325E-5	3.0915707807401325E-5
18-19	4.1220943743201766E-5	2.3702042652341013E-4	0.0	4.1220943743201766E-5	3.0915707807401325E-5
20-21	4.1220943743201766E-5	2.57630898395011E-4	0.0	6.183141561480265E-5	3.0915707807401325E-5
22-23	5.152617967900221E-5	2.988518421382128E-4	0.0	1.0820497732590464E-4	4.1220943743201766E-5
24-25	6.183141561480265E-5	3.400727858814146E-4	0.0	1.3912068513330595E-4	5.152617967900221E-5
26-27	7.213665155060309E-5	3.50378021817215E-4	0.0	1.9064686481230818E-4	5.152617967900221E-5
28-29	7.213665155060309E-5	3.606832577530154E-4	0.0	5.770932124048247E-4	5.152617967900221E-5
30-31	8.244188748640353E-5	3.709884936888159E-4	0.0	0.002055894569192188	5.152617967900221E-5
32-33	8.244188748640353E-5	3.709884936888159E-4	0.0	0.0046631192609497	5.152617967900221E-5
34-35	9.78997413901042E-5	4.1220943743201766E-4	0.0	0.008491514411099563	6.698403358270287E-5
36-37	1.1335759529380486E-4	4.534303811752194E-4	0.0	0.014834387129584736	7.213665155060309E-5
38-39	1.1335759529380486E-4	4.585829991431196E-4	0.0	0.027010023387732958	7.213665155060309E-5
40-41	1.236628312296053E-4	5.35872268661623E-4	0.0	0.06663365556088566	7.213665155060309E-5
42-43	1.236628312296053E-4	5.513301225653236E-4	0.0	0.07784059964106863	8.759450545430375E-5
44-45	1.236628312296053E-4	6.389246280196273E-4	0.0	0.09523583790069978	1.0305235935800442E-4
46-47	1.236628312296053E-4	7.110612795702305E-4	0.0	0.1145839183701651	1.1335759529380486E-4
48-49	1.236628312296053E-4	7.110612795702305E-4	0.0	0.13242228177503568	1.236628312296053E-4
50-51	1.236628312296053E-4	7.522822233134321E-4	0.0	0.15093563813370117	1.236628312296053E-4
52-53	1.236628312296053E-4	7.625874592492326E-4	0.0	0.1841545661727539	1.4427330310120618E-4
54-55	1.236628312296053E-4	7.780453131529333E-4	0.0	0.26373159806900487	1.700363929407073E-4
56-57	1.236628312296053E-4	7.935031670566339E-4	0.0	0.35349535568779467	1.751890109086075E-4
58-59	1.236628312296053E-4	7.935031670566339E-4	0.0	0.4433260973401671	1.751890109086075E-4
60-61	1.3396806716540574E-4	8.244188748640353E-4	0.0	0.7692497942817276	1.803416288765077E-4
62-63	1.3396806716540574E-4	8.501819647035364E-4	0.0	0.7764016280211732	1.8549424684440795E-4
64-65	1.3396806716540574E-4	9.274712342220397E-4	0.0	0.7835070881989075	1.8549424684440795E-4
66-67	1.3396806716540574E-4	9.480817060936406E-4	0.0	0.7899066397150396	1.9064686481230818E-4
68-69	1.3396806716540574E-4	9.635395599973413E-4	0.0	0.7963371069389791	2.0610471871600883E-4
70-71	1.3396806716540574E-4	9.893026498368424E-4	0.0	0.8021338021528668	2.267151905876097E-4
72-73	1.3396806716540574E-4	9.893026498368424E-4	0.0	0.8060807075162784	2.3702042652341016E-4
74-75	1.3396806716540574E-4	0.001009913121708443	0.0	0.8094814353750925	2.3702042652341016E-4
76-77	1.3396806716540574E-4	0.001071744537323246	0.0	0.8122844595496302	2.3702042652341016E-4
78-79	1.3396806716540574E-4	0.0011026602451306472	0.0	0.8148040897359334	2.3702042652341016E-4
80-81	1.3396806716540574E-4	0.0011026602451306472	0.0	0.8159170552169999	2.3702042652341016E-4
82-83	1.3912068513330595E-4	0.0011078128630985474	0.0	0.8163962486880146	2.473256624592106E-4
84-85	1.4427330310120618E-4	0.0011232707170022482	0.0	0.8168909000129331	2.473256624592106E-4
86-87	1.4427330310120618E-4	0.0011335759529380485	0.0	0.8171794466191354	2.473256624592106E-4
88-89	1.4427330310120618E-4	0.0011851021326170507	0.0	0.8172670411245898	2.473256624592106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11645	0.0	42.78062	1
GTATCAA	20160	0.0	35.15029	1
TCAACGC	25840	0.0	28.183538	4
ATCAACG	25885	0.0	28.118256	3
CAACGCA	26700	0.0	27.210203	5
TATCAAC	27215	0.0	26.53555	2
AACGCAG	27780	0.0	26.250057	6
TGGTATC	6215	0.0	23.253471	2
GTGGTAT	6445	0.0	23.101086	1
ACGCAGA	33245	0.0	21.895544	7
CGCAGAG	33510	0.0	21.670412	8
GCAGAGT	39650	0.0	18.38068	9
AGAGTAC	36050	0.0	18.140198	10-11
GAGACCG	10095	0.0	17.75981	88-89
GACCGAG	10340	0.0	17.555618	90-91
ACGAGAC	10465	0.0	17.228952	86-87
CGAGACC	10195	0.0	17.152063	88-89
AGACCGA	10220	0.0	17.050186	90-91
ACCGAGG	10675	0.0	16.853554	92-93
AGTACTT	26085	0.0	16.730143	12-13
>>END_MODULE