FastQCFastQC Report
Fri 27 May 2016
SRR2140389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21711134
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA253450.051.659621
GTATCAA468250.033.132351
ATCAACG562900.030.1699283
TCAACGC562950.030.1511634
CAACGCA581800.029.181665
TATCAAC589900.028.5000422
AACGCAG607250.027.980196
ACGCAGA724600.023.3945067
CGCAGAG732650.023.125758
GAGTACT491250.019.90334512-13
GCAGAGT866950.019.5030149
TGGTATC123500.018.8254952
GTGGTAT125850.018.5781421
CAGAGTA860700.018.19573210-11
GTACTTT549700.017.66005714-15
CGAGACC229850.016.9708888-89
GAGACCG236250.016.94379888-89
GACCGAG239800.016.84632790-91
ACGAGAC240200.016.75476586-87
AGACCGA239400.016.40671390-91
Produced by FastQC (version 0.11.5)