##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21711134
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.597400762208	25.0	18.0	32.0	18.0	33.0
2	27.675413361642004	29.0	25.0	32.0	18.0	33.0
3	29.566927733945175	31.0	28.0	33.0	25.0	33.0
4	30.647698687687157	33.0	31.0	33.0	27.0	33.0
5	31.08758229763586	33.0	32.0	33.0	27.0	33.0
6	34.999679519273386	37.0	34.0	38.0	29.0	38.0
7	35.37750731951634	38.0	35.0	38.0	29.0	38.0
8	36.02886933496887	38.0	37.0	38.0	31.0	38.0
9	36.23269139235196	38.0	37.0	38.0	33.0	38.0
10-11	36.27102414364906	38.0	37.0	38.0	33.0	38.0
12-13	36.24906190989379	38.0	37.0	38.0	33.0	38.0
14-15	36.35115846551359	38.0	37.0	38.0	33.0	38.0
16-17	36.341529535030276	38.0	37.0	38.0	33.0	38.0
18-19	36.36920740759096	38.0	37.0	38.0	33.0	38.0
20-21	36.38073680093714	38.0	37.0	38.0	33.0	38.0
22-23	36.375459271102386	38.0	37.0	38.0	33.0	38.0
24-25	36.3650143409138	38.0	37.0	38.0	33.0	38.0
26-27	36.30212480348154	38.0	37.0	38.0	33.0	38.0
28-29	36.27405837340807	38.0	37.0	38.0	33.0	38.0
30-31	36.270637752311885	38.0	37.0	38.0	33.0	38.0
32-33	36.24300620914828	38.0	37.0	38.0	33.0	38.0
34-35	36.21256533154414	38.0	37.0	38.0	33.0	38.0
36-37	36.185771500008585	38.0	37.0	38.0	33.0	38.0
38-39	36.16801747964861	38.0	37.0	38.0	33.0	38.0
40-41	36.164947856943925	38.0	37.0	38.0	33.0	38.0
42-43	36.115263545046794	38.0	37.0	38.0	33.0	38.0
44-45	36.06405711160649	38.0	37.0	38.0	32.5	38.0
46-47	36.02762607761828	38.0	37.0	38.0	31.0	38.0
48-49	35.97583899437717	38.0	37.0	38.0	31.0	38.0
50-51	35.941346730293844	38.0	37.0	38.0	31.0	38.0
52-53	35.917144090659036	38.0	37.0	38.0	31.0	38.0
54-55	35.87822687088619	38.0	37.0	38.0	31.0	38.0
56-57	35.84170179699535	38.0	37.0	38.0	31.0	38.0
58-59	35.815486119599356	38.0	37.0	38.0	31.0	38.0
60-61	35.81435690747557	38.0	37.0	38.0	31.0	38.0
62-63	35.8073008824838	38.0	37.0	38.0	31.0	38.0
64-65	35.77444015987972	38.0	37.0	38.0	31.0	38.0
66-67	35.75292617509538	38.0	37.0	38.0	30.5	38.0
68-69	35.74504246898911	38.0	37.0	38.0	30.5	38.0
70-71	35.71281642818708	38.0	37.0	38.0	29.5	38.0
72-73	35.6984855388483	38.0	37.0	38.0	29.0	38.0
74-75	35.713922496304704	38.0	37.0	38.0	29.0	38.0
76-77	35.6716099433724	38.0	36.5	38.0	29.0	38.0
78-79	35.67145759775693	38.0	36.0	38.0	29.0	38.0
80-81	35.64149443836263	38.0	36.0	38.0	29.0	38.0
82-83	35.61528630900149	38.0	36.0	38.0	29.0	38.0
84-85	35.593309644810134	38.0	36.0	38.0	29.0	38.0
86-87	35.5623192959257	38.0	36.0	38.0	29.0	38.0
88-89	35.48390028167564	38.0	36.0	38.0	29.0	38.0
90-91	35.49957247919354	38.0	36.0	38.0	29.0	38.0
92-93	35.53828262396219	38.0	36.0	38.0	29.0	38.0
94-95	35.5089063891685	38.0	36.0	38.0	29.0	38.0
96-97	35.50220026588079	38.0	36.0	38.0	29.0	38.0
98-99	35.52902608709316	38.0	36.0	38.0	29.0	38.0
100-101	34.9272240461402	38.0	35.0	38.0	27.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	10.0
18	93.0
19	186.0
20	458.0
21	1986.0
22	7893.0
23	23700.0
24	52753.0
25	97785.0
26	157730.0
27	233878.0
28	322461.0
29	424487.0
30	540388.0
31	678550.0
32	853983.0
33	1099464.0
34	1489586.0
35	2266884.0
36	4634517.0
37	8824342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.88110294008595	27.60936393280977	13.792365705080167	23.71716742202411
2	14.162715506371775	21.137146550573846	39.23281663835784	25.467321304696533
3	17.040285626086625	25.941596698611395	32.3226391210717	24.695478554230274
4	13.562788988973166	19.238589075159748	38.467676070363474	28.73094586550361
5	13.986464466528812	36.653589070265475	35.358588135712566	14.001358327493147
6	29.02090236281532	39.40150707927094	18.522938507035146	13.054652050878596
7	25.532908598878347	33.467169425604396	23.34719595945564	17.65272601606162
8	23.507468564285954	38.06063285317109	20.919902203173727	17.511996379369222
9	24.459952537644224	17.5275357983918	21.69516414667317	36.31734751729081
10-11	23.483789469495235	27.552271106612856	29.02303490918531	19.9409045147066
12-13	24.2860396947953	24.9047936626616	29.42045149323963	21.388715149303465
14-15	21.577280498769287	26.446218145238554	26.879315776673984	25.09718557931817
16-17	21.110323675713126	29.720394312990223	27.669602710886267	21.49967930041039
18-19	21.122406595620475	28.783717607749093	29.41021182956173	20.683663967068693
20-21	21.808912563259767	27.787313791521672	29.470128502199312	20.93364514301925
22-23	21.58673244374335	27.743497867612838	29.295910915312334	21.37385877333148
24-25	21.394531537647417	27.908398707651937	29.370597268852993	21.326472485847653
26-27	21.389582958647775	28.001258091105914	29.273125262375878	21.336033687870433
28-29	21.319692620528745	27.9742447312824	29.343282958157197	21.362779690031665
30-31	21.57489975521679	27.878084704575812	29.29540979473258	21.25160574547482
32-33	21.42806536136352	27.958098546007037	29.26793941342926	21.345896679200184
34-35	21.44965195746176	27.996187202663457	29.305203018216076	21.248957821658706
36-37	21.47418046634552	27.9364565235911	29.236210083556614	21.353152926506763
38-39	21.349953803375094	27.9934114238439	29.32250574339414	21.33412902938687
40-41	21.545178731927194	27.878269198928358	29.225348189656785	21.351203879487667
42-43	21.45529723553185	28.00326734592978	29.057317539085876	21.48411787945249
44-45	21.487544412349802	28.0369197790404	28.928309667794345	21.547226140815457
46-47	21.62094072810001	28.029496591762076	28.708566037506618	21.640996642631297
48-49	21.524034138711645	28.14881284519139	28.699581939557596	21.627571076539372
50-51	21.520679227736405	28.195786042370326	28.645311373196826	21.638223356696447
52-53	21.608713173039483	28.1507669665245	28.61635289977473	21.624166960661288
54-55	21.563340134558505	28.10982252941065	28.601758833258735	21.725078502772114
56-57	21.6006547852756	28.147388634435693	28.57682656479493	21.675130015493774
58-59	21.542631010149346	28.218632608164423	28.66440661582617	21.57432976586006
60-61	21.570952783076596	28.098219343764658	28.71045595894031	21.620371914218435
62-63	21.678287725971387	28.19800811653352	28.678284331615494	21.4454198258796
64-65	21.399009794909972	28.18785933687616	28.63224597531301	21.78088489290086
66-67	21.447581200474662	28.29004280483143	28.478805948077714	21.783570046616198
68-69	21.42905024845491	28.359949850874006	28.524773524541363	21.686226376129724
70-71	21.43996652681307	28.178781472158636	28.562625503987583	21.818626497040704
72-73	21.504197966651393	28.175481698072225	28.336518017574885	21.9838023177015
74-75	21.40258357069449	28.19741640816505	28.374663334824003	22.025336686316464
76-77	21.59951664820946	28.023224529080043	28.44344446139331	21.93381436131719
78-79	21.5804969360784	28.001741531305214	28.375668986646808	22.04209254596958
80-81	21.770243276085885	28.03383570563845	28.19524406743642	22.00067695083924
82-83	21.736041382447127	28.265678564616326	28.244093571588895	21.75418648134765
84-85	21.67117122786625	28.100697101649924	28.206806592293116	22.02132507819071
86-87	21.614836548007524	28.290504717496486	28.224153558538802	21.87050517595719
88-89	21.825734268629155	28.084835139461784	28.22265472160729	21.866775870301772
90-91	21.89120309116359	28.296170772082686	28.215542997750966	21.597083139002756
92-93	21.727392942327434	28.17882663652108	28.236317786364822	21.857462634786664
94-95	21.771009147791382	28.111470057535037	28.308736793517287	21.808784001156294
96-97	21.742590195399625	28.347290927338538	28.315966570741114	21.594152306520726
98-99	22.0112558156574	28.729094161751593	28.482363751816436	20.777286270774567
100-101	22.40384005333908	31.3404600679919	25.971361984363384	20.284337894305633
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4754.0
1	4553.0
2	5451.0
3	6646.5
4	7643.5
5	9783.5
6	12697.5
7	17316.0
8	21770.0
9	23842.5
10	23518.0
11	23263.0
12	25859.5
13	32863.0
14	48286.0
15	66995.0
16	80126.0
17	86609.5
18	87399.0
19	83894.0
20	79605.0
21	77186.0
22	80341.5
23	93388.5
24	116160.5
25	149818.5
26	193059.5
27	241243.0
28	294962.5
29	352864.0
30	408630.0
31	464527.0
32	520994.5
33	576105.5
34	626736.5
35	670262.0
36	717679.0
37	762055.5
38	796582.5
39	822968.0
40	848036.5
41	871298.0
42	882448.5
43	888724.0
44	895490.5
45	900475.0
46	900627.0
47	896200.0
48	880026.5
49	849812.0
50	815947.5
51	778494.0
52	734392.5
53	689623.0
54	642678.5
55	588569.0
56	534174.5
57	476813.5
58	414671.5
59	351452.0
60	287421.5
61	229195.0
62	177085.0
63	132280.0
64	97041.0
65	70132.0
66	50239.5
67	36454.0
68	26610.5
69	19169.0
70	13753.5
71	9613.0
72	6512.5
73	4193.0
74	2640.5
75	1645.5
76	1031.5
77	610.5
78	362.5
79	222.0
80	159.5
81	118.0
82	76.0
83	51.5
84	37.0
85	23.5
86	15.0
87	11.0
88	7.5
89	5.0
90	3.5
91	3.5
92	5.0
93	4.5
94	2.5
95	1.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.605931684637016E-6
3	0.002358237022534152
4	4.5138130509442763E-4
5	0.05028756213286695
6	0.0
7	0.0
8	0.0
9	1.5199574559302155E-4
10-11	0.0
12-13	2.302965842318508E-6
14-15	4.329575783558795E-4
16-17	2.5332624265503593E-5
18-19	0.0
20-21	2.3030140535214477E-6
22-23	0.0
24-25	0.0
26-27	1.059696212279451E-4
28-29	0.0
30-31	1.61445172308472E-5
32-33	0.0
34-35	3.935586123021468E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.3443326473306033E-4
44-45	2.777321946186752E-4
46-47	9.566370305297795E-5
48-49	0.0020502659190078467
50-51	6.113082121883796E-4
52-53	0.005343000103589276
54-55	0.0011218238056657504
56-57	0.002919446542093405
58-59	0.0081987392288069
60-61	0.008675488510368708
62-63	0.007039649948377996
64-65	0.010383739974942262
66-67	0.004598038621226676
68-69	0.004480802811533803
70-71	0.004723899138569189
72-73	0.007076866486185602
74-75	0.005324470595683802
76-77	0.0026541858109145238
78-79	1.6672033985995062E-4
80-81	3.0948089845888287E-4
82-83	0.0
84-85	0.0
86-87	8.850837917481041E-4
88-89	1.1279812297155488E-5
90-91	7.694843702334718E-5
92-93	8.644418335099436E-6
94-95	6.673134070053163E-4
96-97	3.1294735233549655E-4
98-99	6.410956801416125E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	277.0
20-21	906.0
22-23	1800.0
24-25	2729.0
26-27	6003.0
28-29	12560.0
30-31	32580.0
32-33	43186.0
34-35	60474.0
36-37	96353.0
38-39	109495.0
40-41	114251.0
42-43	129566.0
44-45	154462.0
46-47	156046.0
48-49	145243.0
50-51	132409.0
52-53	114251.0
54-55	122966.0
56-57	127201.0
58-59	76954.0
60-61	148409.0
62-63	143250.0
64-65	154821.0
66-67	166834.0
68-69	169175.0
70-71	173938.0
72-73	154655.0
74-75	156490.0
76-77	166336.0
78-79	174488.0
80-81	178595.0
82-83	167988.0
84-85	165956.0
86-87	174160.0
88-89	184518.0
90-91	192196.0
92-93	191676.0
94-95	208435.0
96-97	338614.0
98-99	1918849.0
100-101	1.4742039E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.29628184399088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.54371040725735	68.62594452278606
2	8.61558713948614	13.663680521283123
3	2.423280243502869	5.764713395273351
4	1.015761805246885	3.2218453758087184
5	0.5089169039134659	2.017760912394671
6	0.27895423457337776	1.3272020163783185
7	0.18184502516509252	1.0093744057193152
8	0.11468269765421628	0.727512921265434
9	0.07031003773669689	0.5017792111947675
>10	0.24323828902817363	2.8655580017027353
>50	0.0030329162785441557	0.16337133161798234
>100	6.441197251602323E-4	0.08774690338030036
>500	2.4133638444593163E-5	0.01298345047331065
>1k	1.2046793664381365E-5	0.010527030721913614
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.605931684637017E-6	4.605931684637017E-6
2	0.0	1.3817795053911048E-5	0.0	4.605931684637017E-6	4.605931684637017E-6
3	0.0	1.3817795053911048E-5	0.0	1.8423726738548066E-5	4.605931684637017E-6
4	0.0	5.527118021564419E-5	0.0	2.302965842318508E-5	9.211863369274033E-6
5	0.0	5.987711190028121E-5	0.0	2.302965842318508E-5	9.211863369274033E-6
6	3.224152179245911E-5	5.987711190028121E-5	0.0	2.7635590107822096E-5	9.211863369274033E-6
7	3.224152179245911E-5	5.987711190028121E-5	0.0	3.224152179245911E-5	9.211863369274033E-6
8	3.684745347709613E-5	6.448304358491823E-5	0.0	3.684745347709613E-5	1.3817795053911048E-5
9	3.684745347709613E-5	1.4738981390838453E-4	0.0	5.987711190028121E-5	1.3817795053911048E-5
10-11	3.915041931941464E-5	1.4738981390838453E-4	0.0	7.599787279651078E-5	1.3817795053911048E-5
12-13	4.375635100405165E-5	1.5660167727765854E-4	0.0	1.151482921159254E-4	1.8423726738548063E-5
14-15	5.527118021564419E-5	2.2338768670489528E-4	0.0	1.2436015548519943E-4	2.7635590107822096E-5
16-17	8.751270200810331E-5	2.3259955007416932E-4	0.0	1.2896608716983645E-4	3.454448763477762E-5
18-19	8.751270200810331E-5	2.579321743396729E-4	0.0	1.427838822237475E-4	4.1453385161733146E-5
20-21	8.981566785042181E-5	2.80961832762858E-4	0.0	1.5199574559302152E-4	4.1453385161733146E-5
22-23	9.211863369274032E-5	2.9938555950140603E-4	0.0	1.5890464311997705E-4	4.605931684637016E-5
24-25	9.211863369274032E-5	3.2932411545154663E-4	0.0	1.8193430154316215E-4	5.066524853100718E-5
26-27	9.211863369274032E-5	3.408389446631392E-4	0.0	2.694470035512654E-4	5.987711190028121E-5
28-29	9.211863369274032E-5	3.4544487634777623E-4	0.0	6.931927185378709E-4	5.987711190028121E-5
30-31	9.211863369274032E-5	4.0532198824805743E-4	0.0	0.0018999468199127692	5.987711190028121E-5
32-33	9.442159953505883E-5	4.0532198824805743E-4	0.0	0.004504601187575002	6.908897526955525E-5
34-35	9.672456537737734E-5	4.928346902561608E-4	0.0	0.008373583802670096	7.369490695419227E-5
36-37	9.672456537737734E-5	5.573177338410789E-4	0.0	0.01437050685606749	7.369490695419227E-5
38-39	9.672456537737734E-5	5.7113552889499E-4	0.0	0.02594981911124495	7.369490695419227E-5
40-41	1.0363346290433287E-4	6.586482309030933E-4	0.0	0.06376682120795717	7.369490695419227E-5
42-43	1.0593642874665138E-4	6.931927185378709E-4	0.0	0.07532080083886913	7.369490695419227E-5
44-45	1.0823939458896989E-4	7.968261814422038E-4	0.0	0.09260916541715417	7.369490695419227E-5
46-47	1.128453262736069E-4	8.843388834503071E-4	0.0	0.11215673948675367	8.290677032346629E-5
48-49	1.151482921159254E-4	8.889448151349442E-4	0.0	0.12994254468697952	9.211863369274032E-5
50-51	1.3126905301215496E-4	0.0010501524240972397	0.0	0.14812676297792643	9.211863369274032E-5
52-53	1.381779505391105E-4	0.0010570613216241951	0.0	0.17988696490934097	1.0133049706201436E-4
54-55	1.381779505391105E-4	0.0010731820825204247	0.0	0.2570593502854342	1.1054236043128838E-4
56-57	1.381779505391105E-4	0.001100817672628247	0.0	0.34377292314625296	1.128453262736069E-4
58-59	1.381779505391105E-4	0.0011146354676821579	0.0	0.4274557929585806	1.151482921159254E-4
60-61	1.381779505391105E-4	0.0011399680919476616	0.0	0.7588986369850603	1.151482921159254E-4
62-63	1.6581354064693258E-4	0.0011814214771093947	0.0	0.7663233988606952	1.1975422380056242E-4
64-65	1.681165064892511E-4	0.001259722315748224	0.0	0.7733750802698744	1.2205718964288092E-4
66-67	1.7732836985852513E-4	0.001273540110802135	0.0	0.7796644799852462	1.2436015548519943E-4
68-69	1.7963133570084364E-4	0.0013080845984369126	0.0	0.7857258860822286	1.2436015548519943E-4
70-71	1.8193430154316215E-4	0.0013288112910177792	0.0	0.7913382138399588	1.2436015548519943E-4
72-73	2.1187285749330276E-4	0.0013449320519140088	0.0	0.7951311985822573	1.4738981390838453E-4
74-75	2.1187285749330276E-4	0.0013909913687603788	0.0	0.7983069884788145	1.4738981390838453E-4
76-77	2.1647878917793978E-4	0.001437050685606749	0.0	0.8012478758594553	1.4969277975070301E-4
78-79	2.302965842318508E-4	0.0014577773781876157	0.0	0.8039999200410259	1.5199574559302152E-4
80-81	2.302965842318508E-4	0.0014600803440299342	0.0	0.805229703800824	1.6581354064693258E-4
82-83	2.302965842318508E-4	0.001478504070768482	0.0	0.8057133266277109	1.8884319907011766E-4
84-85	2.302965842318508E-4	0.0015015337291916673	0.0	0.806219979113021	1.8884319907011766E-4
86-87	2.3259955007416932E-4	0.001545290080195719	0.0	0.8064042163804065	1.8884319907011766E-4
88-89	2.3490251591648783E-4	0.0016304998163615037	0.0	0.8064571845947798	1.8884319907011766E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25345	0.0	51.65962	1
GTATCAA	46825	0.0	33.13235	1
ATCAACG	56290	0.0	30.169928	3
TCAACGC	56295	0.0	30.151163	4
CAACGCA	58180	0.0	29.18166	5
TATCAAC	58990	0.0	28.500042	2
AACGCAG	60725	0.0	27.98019	6
ACGCAGA	72460	0.0	23.394506	7
CGCAGAG	73265	0.0	23.12575	8
GAGTACT	49125	0.0	19.903345	12-13
GCAGAGT	86695	0.0	19.503014	9
TGGTATC	12350	0.0	18.825495	2
GTGGTAT	12585	0.0	18.578142	1
CAGAGTA	86070	0.0	18.195732	10-11
GTACTTT	54970	0.0	17.660057	14-15
CGAGACC	22985	0.0	16.97088	88-89
GAGACCG	23625	0.0	16.943798	88-89
GACCGAG	23980	0.0	16.846327	90-91
ACGAGAC	24020	0.0	16.754765	86-87
AGACCGA	23940	0.0	16.406713	90-91
>>END_MODULE