FastQCFastQC Report
Fri 27 May 2016
SRR2140388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197478
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138600.055.4025571
GTATCAA263350.039.8962551
TCAACGC309300.033.545784
ATCAACG309050.033.503993
CAACGCA318350.032.5648165
TATCAAC322500.032.2955552
AACGCAG325950.031.8282496
ACGCAGA367700.028.1788797
CGCAGAG371800.027.9032218
GTGGTAT59650.026.4025861
TGGTATC58900.025.2615012
GCAGAGT434450.023.809379
GAGTACT263150.022.19894412-13
CGAGACC81800.021.04540488-89
GAGACCG83950.021.03647688-89
CAGAGTA426300.020.99585310-11
GACCGAG85700.020.85914290-91
GTACATG303400.020.7205181
TACATGG293050.020.6506522
AGACCGA84700.020.53949590-91
Produced by FastQC (version 0.11.5)