##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9197478
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.71730109058157	32.0	18.0	33.0	18.0	33.0
2	29.80515669621607	31.0	28.0	33.0	25.0	33.0
3	31.035527456548415	33.0	31.0	33.0	27.0	33.0
4	30.89075472645871	33.0	31.0	33.0	27.0	34.0
5	31.298179566181076	33.0	32.0	33.0	28.0	34.0
6	34.11948188405561	37.0	33.0	38.0	26.0	38.0
7	35.564799285195356	38.0	36.0	38.0	30.0	38.0
8	35.36408632888276	38.0	35.0	38.0	29.0	38.0
9	36.04973754761903	38.0	37.0	38.0	31.0	38.0
10-11	36.276093022456806	38.0	37.0	38.0	33.0	38.0
12-13	36.371148754038884	38.0	37.0	38.0	33.0	38.0
14-15	36.36287251787935	38.0	37.0	38.0	33.0	38.0
16-17	36.3906271914975	38.0	37.0	38.0	33.0	38.0
18-19	36.35527842523788	38.0	37.0	38.0	33.0	38.0
20-21	36.39978999471424	38.0	37.0	38.0	33.5	38.0
22-23	36.451090034304656	38.0	37.5	38.0	34.0	38.0
24-25	36.455041501860435	38.0	38.0	38.0	34.0	38.0
26-27	36.405763082044245	38.0	38.0	38.0	34.0	38.0
28-29	36.38326812770359	38.0	37.5	38.0	33.5	38.0
30-31	36.37308005086842	38.0	38.0	38.0	33.0	38.0
32-33	36.35294745202195	38.0	37.0	38.0	33.0	38.0
34-35	36.328935507344525	38.0	37.0	38.0	33.0	38.0
36-37	36.291772207281355	38.0	37.0	38.0	33.0	38.0
38-39	36.287035474739	38.0	37.0	38.0	33.0	38.0
40-41	36.26503975793952	38.0	37.0	38.0	33.0	38.0
42-43	36.22840776716358	38.0	37.0	38.0	33.0	38.0
44-45	36.172161659414414	38.0	37.0	38.0	33.0	38.0
46-47	36.12200939523749	38.0	37.0	38.0	33.0	38.0
48-49	36.07390689578823	38.0	37.0	38.0	33.0	38.0
50-51	36.046301069206336	38.0	37.0	38.0	32.0	38.0
52-53	36.0143030208213	38.0	37.0	38.0	31.0	38.0
54-55	35.92930078822789	38.0	37.0	38.0	31.0	38.0
56-57	35.95068413760428	38.0	37.0	38.0	31.0	38.0
58-59	35.92909786439559	38.0	37.0	38.0	31.0	38.0
60-61	35.92224091917888	38.0	37.0	38.0	31.0	38.0
62-63	35.90694637580862	38.0	37.0	38.0	31.0	38.0
64-65	35.906666357874016	38.0	37.0	38.0	31.0	38.0
66-67	35.904732945668556	38.0	37.0	38.0	31.0	38.0
68-69	35.88282721903721	38.0	37.0	38.0	31.0	38.0
70-71	35.86117599145092	38.0	37.0	38.0	31.0	38.0
72-73	35.85102819305965	38.0	37.0	38.0	31.0	38.0
74-75	35.83592180983615	38.0	37.0	38.0	31.0	38.0
76-77	35.844895538680035	38.0	37.0	38.0	31.0	38.0
78-79	35.808575976107505	38.0	37.0	38.0	31.0	38.0
80-81	35.78827342429699	38.0	37.0	38.0	31.0	38.0
82-83	35.696531671921576	38.0	36.5	38.0	30.0	38.0
84-85	35.719801195491115	38.0	37.0	38.0	30.0	38.0
86-87	35.72457690955649	38.0	37.0	38.0	31.0	38.0
88-89	35.73132558731841	38.0	37.0	38.0	31.0	38.0
90-91	35.70298528821752	38.0	36.5	38.0	30.5	38.0
92-93	35.667949985821465	38.0	36.0	38.0	29.5	38.0
94-95	35.6781448535059	38.0	36.0	38.0	30.5	38.0
96-97	35.6957090441571	38.0	36.0	38.0	31.0	38.0
98-99	35.706619322554005	38.0	36.0	38.0	31.0	38.0
100-101	35.11897451114605	38.0	35.0	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	19.0
20	112.0
21	731.0
22	3051.0
23	9433.0
24	21096.0
25	38351.0
26	62671.0
27	92768.0
28	129048.0
29	170084.0
30	217069.0
31	272402.0
32	340181.0
33	433691.0
34	582717.0
35	869233.0
36	1706911.0
37	4247907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.04419820302913	18.859691754630997	13.80892675144208	21.287183290897786
2	14.770875233406375	20.62362095348312	42.97368256819967	21.631821244910835
3	18.056336594779165	27.530121294122917	30.076410643821323	24.337131467276595
4	12.400334091584671	19.60540704745366	38.82189226220492	29.172366598756742
5	13.985673503290968	37.82561060350465	35.07029668979867	13.11841920340571
6	28.94168379636244	38.77021505243068	19.089298174999712	13.198802976207174
7	25.949809284675645	33.85074691127285	23.024909654581396	17.174534149470105
8	24.123319457790494	37.041947803517445	21.052368921132512	17.782363817559553
9	25.46326286401555	17.59766101098584	21.49981766740839	35.43925845759022
10-11	23.77976875834876	27.394857590308995	28.843580816393366	19.981792834948887
12-13	24.836042010646832	24.94418578658193	29.12327705486221	21.096495147909025
14-15	21.968049284814818	26.277833988839117	27.08694165944186	24.66717506690421
16-17	21.133996407487587	29.50697926156376	27.702824137482263	21.656200193466393
18-19	21.184709547552057	28.42667305102551	29.749878173125282	20.638739228297148
20-21	22.051608902756715	27.4745649773518	29.794428446104643	20.67939767378685
22-23	21.789415357622875	27.321737120295385	29.690291885713517	21.198555636368223
24-25	21.44694061372699	27.50029823031166	29.75402224879683	21.29873890716452
26-27	21.37177446501432	27.638487754237495	29.6971745316853	21.292563249062884
28-29	21.34186154480733	27.696952037732398	29.721388571150655	21.23979784630962
30-31	21.6848441781731	27.54449631018161	29.586165869622334	21.184493642022957
32-33	21.428959117011097	27.697013855314655	29.568168925991507	21.305858101682738
34-35	21.58221877041227	27.717521166425	29.536005805573012	21.164254257589715
36-37	21.553167192897053	27.629734482275776	29.573787405850265	21.243310918976906
38-39	21.399424199726354	27.766212492614713	29.61901915633673	21.215344151322206
40-41	21.700544980069374	27.60603356294708	29.463264333133303	21.230157123850244
42-43	21.577481464423766	27.717199332279673	29.253978061446606	21.45134114184996
44-45	21.571334818187744	27.847863854882824	29.11884154385902	21.461959783070412
46-47	21.66441011545353	27.819910895312187	28.88413127008856	21.631547719145722
48-49	21.581565162691728	27.91251680362145	28.90172621240417	21.604191821282644
50-51	21.558877068040193	28.045372981213077	28.775783288145412	21.619966662601318
52-53	21.775648809466897	27.947640359653096	28.60236475415892	21.674346076721086
54-55	21.72152231156302	27.875073881905916	28.662856011373545	21.74054779515752
56-57	21.649566525854407	27.97072870505839	28.677155363446882	21.70254940564032
58-59	21.678198680744604	27.992743971202362	28.706331483825736	21.622725864227295
60-61	21.645807212277873	27.77656507631058	28.852221386190184	21.725406325221368
62-63	21.74961220806161	27.87812726680438	28.8243533516724	21.547907173461606
64-65	21.53630658370618	27.880872398892052	28.749586738310345	21.833234279091425
66-67	21.622965160186205	27.979535769691253	28.543738385645245	21.853760684477297
68-69	21.53723576502358	28.127651297222044	28.637411257764985	21.69770167998939
70-71	21.595012606862156	27.854153648520757	28.683229973428453	21.867603771188637
72-73	21.60825454542506	27.931381458774023	28.44302384630792	22.017340149492995
74-75	21.468837192680994	27.99596788704648	28.46194973373005	22.07324518654248
76-77	21.694115033364277	27.791337189466255	28.506737124403926	22.007810652765546
78-79	21.670105683530952	27.853133999146134	28.388103331847002	22.088656985475907
80-81	21.878631515874204	27.824272182496994	28.220864374633912	22.076231926994886
82-83	21.881709001602513	27.98855912183671	28.291765794187608	21.83796608237317
84-85	21.851915160978855	27.827909073329643	28.20356930013813	22.11660646555337
86-87	21.643937261349908	28.111141500511287	28.247254024462936	21.99766721367587
88-89	21.97497841562107	27.940623329659957	28.183212760229637	21.901185494489333
90-91	22.03885101324717	28.083456219252987	28.212360485334703	21.665332282165135
92-93	21.876927761270245	28.04471721026766	28.15305571528305	21.925299313179046
94-95	21.933783673642832	27.94779126817215	28.23065300273701	21.887772055448004
96-97	21.89499351085715	28.182846549951996	28.277652566872202	21.644507372318646
98-99	22.130081537188993	28.590605468252058	28.416101551227907	20.863211443331046
100-101	22.544890697605616	31.206433054316367	25.904568580687858	20.34410766739016
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2135.0
1	1880.0
2	2405.0
3	3286.0
4	3841.5
5	5743.0
6	7974.0
7	10806.0
8	14661.5
9	16639.5
10	15302.0
11	13453.5
12	13884.0
13	16805.5
14	23166.0
15	31238.5
16	38524.5
17	44379.0
18	46551.0
19	44445.0
20	40782.5
21	37470.5
22	36820.5
23	40806.0
24	49857.0
25	63801.0
26	82411.0
27	102928.0
28	124022.0
29	145700.0
30	165580.0
31	186829.5
32	210744.5
33	234527.0
34	256751.5
35	275494.0
36	296122.0
37	315595.0
38	329207.5
39	340068.5
40	352465.5
41	363794.5
42	369596.5
43	373961.5
44	377478.5
45	379213.5
46	377163.5
47	372700.5
48	368103.0
49	360106.0
50	349063.0
51	335487.5
52	317168.0
53	296806.5
54	277214.5
55	256606.0
56	235164.0
57	211400.0
58	186651.0
59	161799.5
60	133391.5
61	106051.5
62	81356.5
63	60585.0
64	44838.0
65	32641.0
66	23736.5
67	17679.0
68	13182.0
69	9480.0
70	6721.5
71	4717.5
72	3221.0
73	2066.5
74	1256.0
75	748.5
76	464.0
77	275.0
78	163.5
79	98.5
80	68.0
81	52.0
82	41.5
83	30.0
84	21.0
85	14.0
86	10.0
87	7.0
88	4.5
89	4.0
90	3.5
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003587940085314692
4	0.0
5	0.048295848057478365
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.6308818569612236E-4
18-19	0.0
20-21	0.0
22-23	5.436641999353366E-6
24-25	0.0
26-27	2.2839924971934083E-4
28-29	1.4688533722561343E-4
30-31	5.445221453344852E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.367695273975915E-5
44-45	0.0
46-47	0.0011218416333560913
48-49	0.0017251030077856412
50-51	0.0023251952458965917
52-53	0.005690059668561557
54-55	0.0070079769084245725
56-57	0.006180073611543462
58-59	0.00820263728972099
60-61	0.00741809649982161
62-63	0.005842627716134168
64-65	0.006236954746283392
66-67	0.0014095297577856774
68-69	0.0020100734093516947
70-71	5.75031359304264E-4
72-73	0.0018838005688952963
74-75	0.002189402987553622
76-77	6.093605009095658E-4
78-79	0.0
80-81	1.8120133247694133E-4
82-83	0.0
84-85	6.599792726909619E-6
86-87	5.331470872841754E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	7.523417682455765E-4
96-97	3.530160241739725E-5
98-99	5.770985384796886E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	104.0
20-21	349.0
22-23	859.0
24-25	1239.0
26-27	2764.0
28-29	6161.0
30-31	15403.0
32-33	20134.0
34-35	27669.0
36-37	43395.0
38-39	49642.0
40-41	52675.0
42-43	60604.0
44-45	72945.0
46-47	73053.0
48-49	67612.0
50-51	60866.0
52-53	52482.0
54-55	55902.0
56-57	56977.0
58-59	34576.0
60-61	65342.0
62-63	63148.0
64-65	66482.0
66-67	70288.0
68-69	72078.0
70-71	71867.0
72-73	68614.0
74-75	69170.0
76-77	72706.0
78-79	76624.0
80-81	77740.0
82-83	73911.0
84-85	72755.0
86-87	76053.0
88-89	80296.0
90-91	83060.0
92-93	82935.0
94-95	89745.0
96-97	144448.0
98-99	808149.0
100-101	6156656.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.24349326693397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.64691823700247	73.79279141396361
2	7.74476341517136	12.894023224096273
3	1.9579556966532754	4.889612155539359
4	0.742271507012812	2.4715709278503177
5	0.3573149646005185	1.4872072924949005
6	0.18379417708480225	0.9179801605596243
7	0.11084121548077835	0.6458766982201066
8	0.06789374215269611	0.4521369814203927
9	0.047098886812922416	0.3528608280562433
>10	0.13778410479664863	1.7209538571949585
>50	0.002334231369870207	0.12738714937860612
>100	8.756746886002483E-4	0.12505733560395285
>500	8.808559263374729E-5	0.04084224270252816
>1k	6.606158062656072E-5	0.08169973291914863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0872545713074823E-5	0.0
2	0.0	2.1745091426149647E-5	0.0	1.0872545713074823E-5	0.0
3	0.0	2.1745091426149647E-5	0.0	2.1745091426149647E-5	0.0
4	0.0	4.349018285229929E-5	0.0	2.1745091426149647E-5	1.0872545713074823E-5
5	0.0	5.436272856537412E-5	0.0	3.261763713922447E-5	1.0872545713074823E-5
6	0.0	5.436272856537412E-5	0.0	3.261763713922447E-5	2.1745091426149647E-5
7	0.0	5.436272856537412E-5	0.0	4.349018285229929E-5	2.1745091426149647E-5
8	0.0	7.610781999152376E-5	0.0	6.523527427844895E-5	6.523527427844895E-5
9	0.0	1.6308818569612236E-4	0.0	6.523527427844895E-5	7.610781999152376E-5
10-11	0.0	1.6308818569612236E-4	0.0	7.610781999152377E-5	7.610781999152376E-5
12-13	0.0	1.6308818569612236E-4	0.0	8.698036570459859E-5	7.610781999152376E-5
14-15	0.0	2.0657836854842164E-4	0.0	8.698036570459859E-5	1.0872545713074824E-4
16-17	0.0	2.283234599745713E-4	0.0	1.250342757003605E-4	1.1959800284382305E-4
18-19	0.0	2.391960056876461E-4	0.0	1.467793671265101E-4	1.413430942699727E-4
20-21	0.0	2.500685514007209E-4	0.0	1.5221563998304752E-4	1.6852445855265977E-4
22-23	0.0	2.609410971137958E-4	0.0	1.5221563998304752E-4	2.1201464140495905E-4
24-25	0.0	2.718136428268706E-4	0.0	2.0657836854842167E-4	2.391960056876461E-4
26-27	0.0	2.826861885399454E-4	0.0	2.881224613964828E-4	2.5550482425725835E-4
28-29	0.0	2.826861885399454E-4	0.0	6.577890156410269E-4	2.6637736997033317E-4
30-31	1.0872545713074823E-5	3.3161264424878215E-4	0.0	0.001875514135505407	2.718136428268706E-4
32-33	1.0872545713074823E-5	3.3704891710531953E-4	0.0	0.00476761129518331	2.935587342530202E-4
34-35	1.0872545713074823E-5	3.9141164567069365E-4	0.0	0.00846427683762875	3.1530382567916985E-4
36-37	1.0872545713074823E-5	4.566469199491426E-4	0.0	0.014525721072667964	3.3704891710531953E-4
38-39	1.0872545713074823E-5	4.8382828423182964E-4	0.0	0.02671384481702484	3.8053909995761884E-4
40-41	1.0872545713074823E-5	5.653723770798908E-4	0.0	0.06589306329408996	4.0228419138376847E-4
42-43	1.0872545713074823E-5	5.762449227929656E-4	0.0	0.07758104993564541	4.0228419138376847E-4
44-45	1.0872545713074823E-5	6.197351056452649E-4	0.0	0.09428671642378486	4.131567370968433E-4
46-47	1.0872545713074823E-5	6.523527427844894E-4	0.0	0.11338977924165733	4.294655556664555E-4
48-49	1.0872545713074823E-5	6.523527427844894E-4	0.0	0.13148169530821385	4.675194656622174E-4
50-51	1.0872545713074823E-5	7.284605627760132E-4	0.0	0.14889407726770315	4.675194656622174E-4
52-53	1.0872545713074823E-5	7.284605627760132E-4	0.0	0.18068540093273394	4.8382828423182964E-4
54-55	1.0872545713074823E-5	7.284605627760132E-4	0.0	0.25873397033404155	5.055733756579793E-4
56-57	1.0872545713074823E-5	7.393331084890879E-4	0.0	0.3444911746459193	5.164459213710541E-4
58-59	1.0872545713074823E-5	7.502056542021628E-4	0.0	0.42905239892935865	5.218821942275916E-4
60-61	1.0872545713074823E-5	7.719507456283125E-4	0.0	0.7704340255013384	5.327547399406663E-4
62-63	1.0872545713074823E-5	7.936958370544621E-4	0.0	0.7780991702290563	5.436272856537412E-4
64-65	1.0872545713074823E-5	8.698036570459859E-4	0.0	0.7853511582196772	5.544998313668161E-4
66-67	1.0872545713074823E-5	8.969850213286729E-4	0.0	0.7921899894732012	5.762449227929656E-4
68-69	1.0872545713074823E-5	9.730928413201967E-4	0.0	0.7988059335396073	5.81681195649503E-4
70-71	1.0872545713074823E-5	0.0010057104784594211	0.0	0.8045357651303977	6.142988327887275E-4
72-73	1.0872545713074823E-5	0.001016583024172496	0.0	0.8090859255113194	6.740978342106391E-4
74-75	1.0872545713074823E-5	0.0010274555698855708	0.0	0.812499904865225	6.795341070671764E-4
76-77	1.0872545713074823E-5	0.0010763820255944075	0.0	0.8155768353020252	6.849703799237138E-4
78-79	1.0872545713074823E-5	0.001108999662733632	0.0	0.8184254422788508	6.958429256367887E-4
80-81	1.0872545713074823E-5	0.001108999662733632	0.0	0.8196540399444283	7.12151744206401E-4
82-83	1.0872545713074823E-5	0.001108999662733632	0.0	0.8202194123215081	7.338968356325505E-4
84-85	1.0872545713074823E-5	0.0011144359355901693	0.0	0.8205836426028961	7.393331084890879E-4
86-87	1.0872545713074823E-5	0.0011198722084467068	0.0	0.8207576033343054	7.393331084890879E-4
88-89	1.0872545713074823E-5	0.0011851074827251558	0.0	0.8208010935171577	7.502056542021628E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13860	0.0	55.402557	1
GTATCAA	26335	0.0	39.896255	1
TCAACGC	30930	0.0	33.54578	4
ATCAACG	30905	0.0	33.50399	3
CAACGCA	31835	0.0	32.564816	5
TATCAAC	32250	0.0	32.295555	2
AACGCAG	32595	0.0	31.828249	6
ACGCAGA	36770	0.0	28.178879	7
CGCAGAG	37180	0.0	27.903221	8
GTGGTAT	5965	0.0	26.402586	1
TGGTATC	5890	0.0	25.261501	2
GCAGAGT	43445	0.0	23.80937	9
GAGTACT	26315	0.0	22.198944	12-13
CGAGACC	8180	0.0	21.045404	88-89
GAGACCG	8395	0.0	21.036476	88-89
CAGAGTA	42630	0.0	20.995853	10-11
GACCGAG	8570	0.0	20.859142	90-91
GTACATG	30340	0.0	20.720518	1
TACATGG	29305	0.0	20.650652	2
AGACCGA	8470	0.0	20.539495	90-91
>>END_MODULE