FastQCFastQC Report
Fri 27 May 2016
SRR2140387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13610460
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205950.054.274521
GTATCAA359450.035.0084841
TCAACGC437600.028.8348454
ATCAACG440100.028.6525733
CAACGCA450050.028.0371695
TATCAAC451450.027.9322132
AACGCAG466300.027.102746
GTGGTAT93150.026.6246131
TGGTATC92550.026.514152
ACGCAGA541400.023.3190167
CGCAGAG549000.022.9564598
GAGTACT363800.020.134412-13
GCAGAGT635300.019.844899
CAGAGTA623300.019.0367210-11
CGAGACC126700.018.90781288-89
GAGACCG128950.018.7491288-89
ACGAGAC131150.018.5537986-87
GACCGAG131950.018.35476990-91
GTACTTT398500.018.32110614-15
AGACCGA130050.018.30073290-91
Produced by FastQC (version 0.11.5)