##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13610460
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.002462076961397	30.0	18.0	32.0	18.0	33.0
2	27.744181827800087	29.0	25.0	32.0	18.0	33.0
3	30.45178208524914	32.0	29.0	33.0	25.0	33.0
4	30.126795126689327	31.0	29.0	33.0	25.0	33.0
5	31.628176564201357	33.0	32.0	33.0	29.0	33.0
6	35.10346505555286	37.0	35.0	38.0	29.0	38.0
7	35.50752590287176	38.0	36.0	38.0	29.0	38.0
8	35.96935019095607	38.0	37.0	38.0	31.0	38.0
9	36.23514708540343	38.0	37.0	38.0	33.0	38.0
10-11	36.360464378132704	38.0	37.0	38.0	33.0	38.0
12-13	36.43643036311778	38.0	37.5	38.0	33.5	38.0
14-15	36.44268018861963	38.0	38.0	38.0	34.0	38.0
16-17	36.48725730063495	38.0	38.0	38.0	34.0	38.0
18-19	36.43638818967177	38.0	38.0	38.0	33.5	38.0
20-21	36.47063105445214	38.0	38.0	38.0	34.0	38.0
22-23	36.524259869004446	38.0	38.0	38.0	34.0	38.0
24-25	36.54293919219781	38.0	38.0	38.0	34.0	38.0
26-27	36.49496063805867	38.0	38.0	38.0	34.0	38.0
28-29	36.4659884794915	38.0	38.0	38.0	34.0	38.0
30-31	36.4540736699981	38.0	38.0	38.0	34.0	38.0
32-33	36.42993600646294	38.0	38.0	38.0	34.0	38.0
34-35	36.41139022266971	38.0	38.0	38.0	34.0	38.0
36-37	36.38111470941404	38.0	38.0	38.0	33.5	38.0
38-39	36.37177226332612	38.0	38.0	38.0	33.0	38.0
40-41	36.357889941986514	38.0	38.0	38.0	33.0	38.0
42-43	36.32297558745611	38.0	38.0	38.0	33.0	38.0
44-45	36.270915192933245	38.0	37.0	38.0	33.0	38.0
46-47	36.22538261908831	38.0	37.0	38.0	33.0	38.0
48-49	36.180156594838515	38.0	37.0	38.0	33.0	38.0
50-51	36.157690412476484	38.0	37.0	38.0	33.0	38.0
52-53	36.12935051035559	38.0	37.0	38.0	33.0	38.0
54-55	36.04906147716038	38.0	37.0	38.0	31.5	38.0
56-57	36.07930157344079	38.0	37.0	38.0	33.0	38.0
58-59	36.063255726941584	38.0	37.0	38.0	32.5	38.0
60-61	36.06067151342208	38.0	37.0	38.0	32.5	38.0
62-63	36.04572822857416	38.0	37.0	38.0	32.0	38.0
64-65	36.05057507889329	38.0	37.0	38.0	32.0	38.0
66-67	36.04634458723215	38.0	37.0	38.0	32.0	38.0
68-69	36.02515963971557	38.0	37.0	38.0	31.0	38.0
70-71	36.007821055176635	38.0	37.0	38.0	31.0	38.0
72-73	35.994732538945144	38.0	37.0	38.0	31.0	38.0
74-75	35.98561425803901	38.0	37.0	38.0	31.0	38.0
76-77	35.99678182723514	38.0	37.0	38.0	31.0	38.0
78-79	35.963793041680255	38.0	37.0	38.0	31.0	38.0
80-81	35.94712949992319	38.0	37.0	38.0	31.0	38.0
82-83	35.85726499606683	38.0	37.0	38.0	31.0	38.0
84-85	35.881176027869216	38.0	37.0	38.0	31.0	38.0
86-87	35.88980595337539	38.0	37.0	38.0	31.0	38.0
88-89	35.90046202893578	38.0	37.0	38.0	31.0	38.0
90-91	35.87345859515023	38.0	37.0	38.0	31.0	38.0
92-93	35.83882894681498	38.0	37.0	38.0	31.0	38.0
94-95	35.84652073264247	38.0	37.0	38.0	31.0	38.0
96-97	35.86627789600383	38.0	37.0	38.0	31.0	38.0
98-99	35.87775345322557	38.0	37.0	38.0	31.0	38.0
100-101	35.31045550705486	38.0	35.5	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	23.0
19	59.0
20	169.0
21	830.0
22	3668.0
23	11377.0
24	26683.0
25	50682.0
26	83984.0
27	126616.0
28	177692.0
29	236862.0
30	305351.0
31	384282.0
32	483444.0
33	618039.0
34	837049.0
35	1258146.0
36	2559483.0
37	6446018.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.412304947812196	23.29365061871531	14.197771419922619	23.096273013549872
2	15.747895368709067	21.314606559954623	40.57186898899817	22.365629082338142
3	18.329027512457685	27.144900932565786	29.800260413930445	24.725811141046083
4	13.457171910427714	18.895702276043572	38.35262731751903	29.294498496009684
5	13.823125065147975	37.49130931516448	35.51225899291999	13.173306626767559
6	29.514314725586054	38.6152782492289	18.49570110047713	13.374705924707907
7	26.13894019746577	33.35137827817722	22.851821319779052	17.65786020457795
8	23.981408416761816	37.65642748298001	20.68372413570151	17.678439964556674
9	24.963395799995002	17.632820639419975	21.415955081606352	35.98782847897867
10-11	23.939532536005395	27.433275583632	28.555177415017567	20.07201446534504
12-13	24.818231712961943	24.805021285099844	29.032207581521856	21.344539420416357
14-15	21.993081056775452	26.30330275391133	26.67528503812509	25.028331151188134
16-17	21.313749640487362	29.535556144237663	27.463639216059114	21.68705499921586
18-19	21.347088930131676	28.465926206755686	29.364797369082307	20.822187494030324
20-21	22.146738915802285	27.423982587203493	29.512655581149478	20.91662291584475
22-23	21.942693460691977	27.292477962880902	29.381326530709718	21.38350204571741
24-25	21.675432893494158	27.52773297860595	29.385910976334955	21.410923151564944
26-27	21.591934489814488	27.622642352527194	29.3523481631593	21.433074994499023
28-29	21.49827602469726	27.61283585427518	29.41173400103941	21.47715411998815
30-31	21.82351636215005	27.46747859652195	29.336386409347888	21.37261863198011
32-33	21.609141626732338	27.6261106033183	29.299873840386198	21.464873929563165
34-35	21.72205104337436	27.650557109807544	29.290344558897846	21.337047287920253
36-37	21.710914229101842	27.566064894086285	29.26693409918798	21.456086777623888
38-39	21.567812231188302	27.654863466930806	29.34578447399485	21.431539827886038
40-41	21.7735963467585	27.514175026201528	29.26859185506812	21.443636771971853
42-43	21.680944928575315	27.64127017799918	29.04492892913299	21.632855964292514
44-45	21.700329008221303	27.720130584729453	28.955925418134033	21.623614988915214
46-47	21.843817514036047	27.681445075821948	28.685773230366053	21.78896417977595
48-49	21.73600545899385	27.796881861507668	28.661403443276534	21.805709236221947
50-51	21.725206265991233	27.91314142607518	28.579697722946566	21.781954584987023
52-53	21.880460561778264	27.858840706326216	28.477749560229366	21.782949171666154
54-55	21.85266116607379	27.761390647150485	28.48026319093821	21.90568499583751
56-57	21.795139413773292	27.86302953563704	28.49663049880749	21.845200551782177
58-59	21.777501545359506	27.896850885499248	28.54271157411756	21.78293599502368
60-61	21.82518101751391	27.766518989451182	28.60570263294392	21.80259736009099
62-63	21.90249511015987	27.87357845828653	28.5691550754319	21.654771356121692
64-65	21.69257691534892	27.85460986229357	28.52495479725074	21.927858425106773
66-67	21.720195147279945	27.935864201071176	28.376821290590286	21.967119361058593
68-69	21.664225403137827	28.054848151761785	28.402870234287196	21.87805621081319
70-71	21.675653399228427	27.84220861687215	28.474542173352713	22.00759581054671
72-73	21.724948516811253	27.883784275229	28.24858889588368	22.142678312076068
74-75	21.652440298869845	27.97402729013556	28.204298101344662	22.169234309649934
76-77	21.856044135532958	27.7227976984584	28.302743363600552	22.118414802408093
78-79	21.842534048149986	27.705872682165243	28.214156485117343	22.237436784567425
80-81	22.022381186135217	27.772163063670945	28.0341875523624	22.171268197831438
82-83	22.02696463576607	27.948033250993948	28.10414817080092	21.920853942439063
84-85	21.920983620561447	27.799435568894708	28.054089024777483	22.22549178576636
86-87	21.810607657414703	28.00767200741991	28.096993343109055	22.084726992056332
88-89	22.072702661798598	27.775436870269814	28.040945735033784	22.1109147328978
90-91	22.15107803130812	27.95627788738176	28.05004010358224	21.842603977727883
92-93	22.002214664953566	27.907848465958008	28.03238315560434	22.057553713484086
94-95	22.051386921183582	27.826581927831572	28.085979130134398	22.03605202085045
96-97	22.039469630195768	28.05501280447254	28.069491393236305	21.836026172095384
98-99	22.304207090162656	28.443593757246767	28.25431364105151	20.997885511539067
100-101	22.718040687445924	31.035701275520346	25.727864881168443	20.51839315586529
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3276.0
1	2854.0
2	3237.0
3	4198.5
4	4964.5
5	6823.5
6	9164.0
7	13352.0
8	18059.5
9	20629.5
10	19872.0
11	18096.5
12	19392.5
13	23820.0
14	33649.0
15	45916.5
16	54747.5
17	59298.5
18	60165.5
19	57578.5
20	53134.5
21	49284.5
22	48904.5
23	54842.5
24	67610.0
25	86869.5
26	111317.0
27	139201.5
28	170282.5
29	203932.5
30	236631.0
31	269539.5
32	303985.5
33	338422.0
34	370965.5
35	398791.5
36	429256.5
37	459370.0
38	481661.0
39	499797.5
40	517014.5
41	532697.0
42	542419.5
43	549139.5
44	554923.0
45	558537.5
46	560128.0
47	559913.5
48	554313.5
49	540923.0
50	524164.5
51	503518.0
52	477282.5
53	449479.5
54	421413.0
55	390152.0
56	356398.0
57	320347.0
58	283710.0
59	244957.0
60	202237.0
61	162008.0
62	126182.0
63	94891.5
64	69373.5
65	49845.5
66	35956.5
67	26840.0
68	20046.5
69	14219.5
70	9893.0
71	6692.0
72	4295.0
73	2657.0
74	1684.5
75	1077.0
76	717.0
77	452.5
78	288.0
79	188.0
80	126.5
81	86.0
82	57.5
83	35.0
84	24.5
85	20.0
86	13.0
87	7.5
88	5.5
89	3.0
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.004327553954825921
4	0.0
5	0.05006443573545641
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.5061945004063052E-4
18-19	0.0
20-21	3.673709633370391E-6
22-23	0.0
24-25	0.0
26-27	3.674652715921124E-4
28-29	1.8012033876592206E-4
30-31	1.103698630232741E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.1292010796969044E-4
44-45	0.0
46-47	0.0010464148376430093
48-49	0.001724187166982678
50-51	0.0024342265588149256
52-53	0.0057223276213165634
54-55	0.007203784388065197
56-57	0.006148173832448166
58-59	0.007997979675352781
60-61	0.007315803396544323
62-63	0.006400762430515844
64-65	0.006208499642555656
66-67	0.0013878834104459046
68-69	0.002074825109533476
70-71	4.7345364122605054E-4
72-73	0.001776009140750442
74-75	0.0024532229290122154
76-77	6.429464284687822E-4
78-79	4.29587624233519E-6
80-81	1.86466172456145E-4
82-83	0.0
84-85	0.0
86-87	9.360011445956854E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	7.061158411839556E-4
96-97	2.8223361944296112E-5
98-99	6.271377680205729E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	474.0
22-23	1006.0
24-25	1505.0
26-27	3615.0
28-29	8087.0
30-31	20933.0
32-33	27713.0
34-35	39227.0
36-37	61847.0
38-39	70359.0
40-41	72278.0
42-43	83695.0
44-45	101145.0
46-47	102430.0
48-49	95057.0
50-51	84530.0
52-53	73914.0
54-55	79336.0
56-57	82097.0
58-59	49806.0
60-61	92996.0
62-63	89215.0
64-65	95134.0
66-67	102472.0
68-69	104543.0
70-71	106071.0
72-73	95435.0
74-75	97251.0
76-77	102363.0
78-79	108146.0
80-81	109381.0
82-83	103242.0
84-85	101184.0
86-87	105573.0
88-89	113440.0
90-91	117060.0
92-93	114969.0
94-95	124452.0
96-97	206855.0
98-99	1199369.0
100-101	9262123.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.46450555482228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.01601280157986	72.58196976589191
2	8.169047536093041	13.473129318355037
3	2.0689667259135542	5.118489541855222
4	0.7872885616636732	2.5969344786624813
5	0.3669410584475408	1.5129806476319798
6	0.1941364678710075	0.9605620699885365
7	0.111484953618381	0.6435486103859459
8	0.07927597423398322	0.5229963214065807
9	0.0499177837857609	0.3704800822456768
>10	0.15332222031759268	1.9129728328793352
>50	0.002737624108810612	0.1502263560324375
>100	7.728848661636901E-4	0.10176924159757096
>500	7.951406144145697E-5	0.03965470687902581
>1k	1.5893439190659594E-5	0.014286026188214824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.347290245884415E-6	0.0	0.0	0.0
3	0.0	7.347290245884415E-6	0.0	0.0	0.0
4	0.0	5.143103172119091E-5	0.0	7.347290245884415E-6	0.0
5	0.0	5.143103172119091E-5	0.0	7.347290245884415E-6	0.0
6	5.877832196707532E-5	5.143103172119091E-5	0.0	7.347290245884415E-6	2.2041870737653246E-5
7	5.877832196707532E-5	5.143103172119091E-5	0.0	7.347290245884415E-6	2.2041870737653246E-5
8	5.877832196707532E-5	5.877832196707532E-5	0.0	2.2041870737653246E-5	2.2041870737653246E-5
9	5.877832196707532E-5	1.1020935368826623E-4	0.0	2.938916098353766E-5	3.6736451229422075E-5
10-11	5.877832196707532E-5	1.1020935368826623E-4	0.0	5.877832196707533E-5	4.408374147530649E-5
12-13	5.877832196707532E-5	1.1020935368826623E-4	0.0	6.979925733590194E-5	5.143103172119091E-5
14-15	5.877832196707532E-5	1.5429309516357272E-4	0.0	7.714654758178636E-5	6.245196709001753E-5
16-17	9.918841831943961E-5	1.5429309516357272E-4	0.0	8.082019270472857E-5	8.082019270472857E-5
18-19	1.0286206344238182E-4	1.7633496590122597E-4	0.0	8.082019270472857E-5	9.551477319649741E-5
20-21	1.0286206344238182E-4	1.9837683663887921E-4	0.0	8.082019270472857E-5	1.1020935368826623E-4
22-23	1.0286206344238182E-4	2.020504817618214E-4	0.0	1.1388299881120844E-4	1.2490393418003505E-4
24-25	1.0286206344238182E-4	2.387869329912435E-4	0.0	1.5061945004063052E-4	1.3225122442591948E-4
26-27	1.0286206344238182E-4	2.6450244885183895E-4	0.0	2.3143964274535908E-4	1.3225122442591948E-4
28-29	1.0286206344238182E-4	2.6817609397478115E-4	0.0	6.53908831883713E-4	1.3225122442591948E-4
30-31	1.0653570856532403E-4	3.4164899643362534E-4	0.0	0.0018515171419628727	1.3959851467180387E-4
32-33	1.1020935368826623E-4	3.4532264155656754E-4	0.0	0.004088767021834677	1.6164038540945715E-4
34-35	1.1020935368826623E-4	4.07774608646585E-4	0.0	0.0073950476324826635	1.7266132077828377E-4
36-37	1.1020935368826623E-4	4.7022657573660256E-4	0.0	0.012714485770502982	1.7633496590122597E-4
38-39	1.1020935368826623E-4	4.885948013513136E-4	0.0	0.02344152952949423	1.7633496590122597E-4
40-41	1.1755664393415064E-4	5.730886391789844E-4	0.0	0.058480756712117006	1.7633496590122597E-4
42-43	1.1755664393415064E-4	5.951305099166377E-4	0.0	0.06851348154287218	1.7633496590122597E-4
44-45	1.2123028905709285E-4	7.090135087278461E-4	0.0	0.0829913169723874	1.7633496590122597E-4
46-47	1.3225122442591948E-4	8.045282819243435E-4	0.0	0.09972109686226623	1.910295463929948E-4
48-49	1.3225122442591948E-4	8.1554921729317E-4	0.0	0.11502550244444346	2.1307141713064804E-4
50-51	1.432721597947461E-4	9.404531514732051E-4	0.0	0.13035194989735835	2.1674506225359026E-4
52-53	1.469458049176883E-4	9.441267965961473E-4	0.0	0.15743038809856535	2.2776599762241686E-4
54-55	1.5061945004063052E-4	9.514740868420318E-4	0.0	0.22512832042414438	2.387869329912435E-4
56-57	1.5429309516357272E-4	9.624950222108584E-4	0.0	0.3015254444008505	2.424605781141857E-4
58-59	1.5429309516357272E-4	9.698423124567428E-4	0.0	0.3760122729136267	2.4980786836007016E-4
60-61	1.5429309516357272E-4	9.771896027026272E-4	0.0	0.7041459289399477	2.5715515860595456E-4
62-63	1.836822561471104E-4	9.91884183194396E-4	0.0	0.7109421724173908	2.791970293436078E-4
64-65	1.836822561471104E-4	0.0011241354076203156	0.0	0.7176649429923749	2.791970293436078E-4
66-67	1.836822561471104E-4	0.0011351563429891423	0.0	0.7234693022866237	2.791970293436078E-4
68-69	1.836822561471104E-4	0.0011939346649562175	0.0	0.7292185569040281	2.791970293436078E-4
70-71	1.9470319151593702E-4	0.001201281955202102	0.0	0.7343616600761473	2.865443195894922E-4
72-73	2.2041870737653246E-4	0.0012123028905709286	0.0	0.7383365440991707	3.0858619032714545E-4
74-75	2.2041870737653246E-4	0.0012306711161856397	0.0	0.7416575192903105	3.1593348057302984E-4
76-77	2.2409235249947466E-4	0.0012857757930297728	0.0	0.7445743935179266	3.1593348057302984E-4
78-79	2.2776599762241689E-4	0.0013151649540133104	0.0	0.7472671753930433	3.232807708189143E-4
80-81	2.2776599762241689E-4	0.0013151649540133104	0.0	0.7484170263165242	3.3797535131068314E-4
82-83	2.2776599762241689E-4	0.0013335331796280213	0.0	0.7488982738276296	3.5266993180245194E-4
84-85	2.2776599762241689E-4	0.0013665959857345014	0.0	0.7493023747911532	3.5634357692539414E-4
86-87	2.2776599762241689E-4	0.0013886378564721546	0.0	0.749449320596071	3.6001722204833633E-4
88-89	2.424605781141857E-4	0.0014768053394227675	0.0	0.7494897306924233	3.6001722204833633E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20595	0.0	54.27452	1
GTATCAA	35945	0.0	35.008484	1
TCAACGC	43760	0.0	28.834845	4
ATCAACG	44010	0.0	28.652573	3
CAACGCA	45005	0.0	28.037169	5
TATCAAC	45145	0.0	27.932213	2
AACGCAG	46630	0.0	27.10274	6
GTGGTAT	9315	0.0	26.624613	1
TGGTATC	9255	0.0	26.51415	2
ACGCAGA	54140	0.0	23.319016	7
CGCAGAG	54900	0.0	22.956459	8
GAGTACT	36380	0.0	20.1344	12-13
GCAGAGT	63530	0.0	19.84489	9
CAGAGTA	62330	0.0	19.03672	10-11
CGAGACC	12670	0.0	18.907812	88-89
GAGACCG	12895	0.0	18.74912	88-89
ACGAGAC	13115	0.0	18.55379	86-87
GACCGAG	13195	0.0	18.354769	90-91
GTACTTT	39850	0.0	18.321106	14-15
AGACCGA	13005	0.0	18.300732	90-91
>>END_MODULE