FastQCFastQC Report
Fri 27 May 2016
SRR2140386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8500537
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122950.054.090521
GTATCAA233000.038.9453051
ATCAACG299750.030.1000883
TCAACGC300900.030.0416764
TATCAAC309750.029.3371892
CAACGCA308050.029.3303955
AACGCAG317100.028.5332666
GTGGTAT55050.024.9135191
ACGCAGA363300.024.8928937
CGCAGAG368650.024.4848678
TGGTATC54800.024.1614572
GCAGAGT421750.021.2896989
CGAGACC89400.021.06690288-89
GAGACCG92150.021.01953788-89
ACGAGAC91300.020.9346786-87
GACCGAG93250.020.8525290-91
ACCGAGG94950.020.68054692-93
AGACCGA92400.020.54918590-91
CACGAGA93950.019.8400286-87
GAGTACT239550.019.7939212-13
Produced by FastQC (version 0.11.5)