##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140386_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8500537
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.388389933483026	31.0	25.0	33.0	18.0	33.0
2	30.93355372725276	33.0	31.0	33.0	27.0	33.0
3	31.154773398433534	33.0	31.0	33.0	27.0	34.0
4	31.169451412304895	33.0	31.0	33.0	28.0	34.0
5	31.325324976527952	33.0	32.0	33.0	28.0	34.0
6	34.796892008116664	37.0	34.0	38.0	29.0	38.0
7	35.235527590786326	38.0	35.0	38.0	29.0	38.0
8	35.52613323134762	38.0	36.0	38.0	29.0	38.0
9	36.14171481166425	38.0	37.0	38.0	33.0	38.0
10-11	36.22746380611014	38.0	37.0	38.0	33.0	38.0
12-13	36.336485389099536	38.0	37.0	38.0	33.0	38.0
14-15	36.31899872913911	38.0	37.0	38.0	33.0	38.0
16-17	36.37477849928776	38.0	37.0	38.0	33.0	38.0
18-19	36.35800114745692	38.0	37.0	38.0	33.0	38.0
20-21	36.414199386812285	38.0	37.5	38.0	33.5	38.0
22-23	36.47549197225855	38.0	38.0	38.0	34.0	38.0
24-25	36.48204632780316	38.0	38.0	38.0	34.0	38.0
26-27	36.44500589942767	38.0	38.0	38.0	34.0	38.0
28-29	36.427175733513394	38.0	38.0	38.0	34.0	38.0
30-31	36.41590640427866	38.0	38.0	38.0	34.0	38.0
32-33	36.391358840370145	38.0	38.0	38.0	34.0	38.0
34-35	36.3664305930329	38.0	38.0	38.0	33.0	38.0
36-37	36.33479714567988	38.0	38.0	38.0	33.0	38.0
38-39	36.334930409705855	38.0	38.0	38.0	33.0	38.0
40-41	36.31234431604226	38.0	38.0	38.0	33.0	38.0
42-43	36.28290219709139	38.0	37.0	38.0	33.0	38.0
44-45	36.22865743752567	38.0	37.0	38.0	33.0	38.0
46-47	36.18049097776262	38.0	37.0	38.0	33.0	38.0
48-49	36.136092631129785	38.0	37.0	38.0	33.0	38.0
50-51	36.11363145235943	38.0	37.0	38.0	33.0	38.0
52-53	36.07816637672976	38.0	37.0	38.0	33.0	38.0
54-55	35.99242023897494	38.0	37.0	38.0	31.0	38.0
56-57	36.020406381974134	38.0	37.0	38.0	31.0	38.0
58-59	36.00494006093402	38.0	37.0	38.0	31.0	38.0
60-61	35.99817128227511	38.0	37.0	38.0	31.0	38.0
62-63	35.98218872027809	38.0	37.0	38.0	31.0	38.0
64-65	35.98346089994858	38.0	37.0	38.0	31.0	38.0
66-67	35.976604421272256	38.0	37.0	38.0	31.0	38.0
68-69	35.95902385710966	38.0	37.0	38.0	31.0	38.0
70-71	35.943629175127136	38.0	37.0	38.0	31.0	38.0
72-73	35.92921907334208	38.0	37.0	38.0	31.0	38.0
74-75	35.911540398763535	38.0	37.0	38.0	31.0	38.0
76-77	35.91690785292373	38.0	37.0	38.0	31.0	38.0
78-79	35.886972922791756	38.0	37.0	38.0	31.0	38.0
80-81	35.869708839475535	38.0	37.0	38.0	31.0	38.0
82-83	35.78012880546859	38.0	37.0	38.0	30.5	38.0
84-85	35.80097431580852	38.0	37.0	38.0	31.0	38.0
86-87	35.806538667434154	38.0	37.0	38.0	31.0	38.0
88-89	35.81361757625304	38.0	37.0	38.0	31.0	38.0
90-91	35.788962417836316	38.0	37.0	38.0	31.0	38.0
92-93	35.75741058093287	38.0	37.0	38.0	31.0	38.0
94-95	35.76721834269176	38.0	37.0	38.0	31.0	38.0
96-97	35.782974512149366	38.0	37.0	38.0	31.0	38.0
98-99	35.796270334723495	38.0	37.0	38.0	31.0	38.0
100-101	35.235014860818254	38.0	35.5	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	21.0
20	89.0
21	599.0
22	2711.0
23	8220.0
24	18309.0
25	33595.0
26	54960.0
27	81435.0
28	113957.0
29	150673.0
30	193825.0
31	243548.0
32	306462.0
33	390816.0
34	524858.0
35	775563.0
36	1495332.0
37	4105559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.41256452386479	20.22375762848865	16.17269591321113	23.190981934435438
2	14.342999742251578	21.877300222327133	40.854960104285176	22.924739931136116
3	17.847377497040647	26.2215590714624	31.791417622393546	24.139645809103403
4	12.571617534280483	18.350064237118197	38.823782544561595	30.254535684039723
5	13.393745247155001	37.480507524104524	35.33208328826431	13.793663940476167
6	29.108305640961934	38.731545869578106	18.695785771626635	13.464362717833323
7	25.830344600582293	33.37822069358677	23.079741903364457	17.71169280246648
8	24.327239561453588	36.942583745003404	20.84972984647911	17.880446847063897
9	25.299425201019655	17.16096288975626	21.376649498731666	36.16296241049242
10-11	24.028664306737326	27.14262640113207	28.658354172212885	20.17035511991772
12-13	24.944135882238967	24.730884648816893	29.052793958781663	21.272185510162476
14-15	22.063894316323783	25.97645889900838	26.925281308698494	25.034365475969338
16-17	21.285694933964947	29.167503624923807	27.524340118582106	22.022461322529136
18-19	21.38486074468001	28.077932017706647	29.651685534690337	20.885521702923004
20-21	22.29907993674841	27.100594040979185	29.59450619589959	21.005819826372818
22-23	22.055776497765926	26.969542759920795	29.491356567001393	21.483324175311886
24-25	21.690903189669307	27.210709703023934	29.558219573173712	21.540167534133047
26-27	21.62485401739882	27.33429180139117	29.490044704450252	21.55080947675976
28-29	21.574467003008426	27.350621098927284	29.51907539058113	21.55583650748316
30-31	21.89398099808378	27.20157186528485	29.394847185656403	21.509599950974962
32-33	21.686624356046366	27.357324754310625	29.361741043629824	21.594309846013186
34-35	21.76586997759173	27.37492742336413	29.40115995946777	21.458042639576362
36-37	21.77376823432918	27.255029510278867	29.39307197095104	21.57813028444092
38-39	21.629114881249254	27.388553263967797	29.411983704034633	21.570348150748316
40-41	21.824376262399113	27.266449851097637	29.340055385618886	21.56911850088437
42-43	21.74043291547407	27.332572048414335	29.157155901688313	21.769839134423286
44-45	21.7678912570029	27.45877574807423	28.992141230401884	21.781191764520983
46-47	21.887315309608468	27.489279577246766	28.680298780294077	21.943106332850686
48-49	21.81845813044805	27.632129213014373	28.630346247680272	21.919066408857308
50-51	21.805870771837437	27.704482594839547	28.516240931483434	21.97340570183958
52-53	21.945280129945203	27.645010900739752	28.38234183699625	22.027367132318794
54-55	21.95372588857012	27.59886615967213	28.360826439661274	22.08658151209648
56-57	21.906981444110706	27.65305467716323	28.415656172192588	22.024307706533474
58-59	21.92301939137897	27.66384791791991	28.453104880503783	21.96002781019734
60-61	21.844847158885482	27.49857749625987	28.61444797033335	22.042127374521296
62-63	21.99212920386777	27.60568765445385	28.516088437139874	21.886094704538507
64-65	21.72051180400753	27.62346166365246	28.43892340800323	22.217103124336784
66-67	21.79367291600314	27.70100143175712	28.314300888242855	22.191024763996882
68-69	21.761321287449956	27.808776380477212	28.35569770091315	22.07420463115968
70-71	21.757837113317184	27.6343165805791	28.41799908946992	22.189847216633794
72-73	21.828177219104575	27.673833870203623	28.167597486847146	22.330391423844656
74-75	21.736665675995106	27.769972975401398	28.164221090245572	22.329140258357928
76-77	21.92945878292411	27.48061838627246	28.23005148288028	22.35987134792315
78-79	21.922131348889245	27.492884933101774	28.1343669731483	22.45061674486068
80-81	22.159123697157668	27.51414070789815	27.96803117830707	22.358704416637114
82-83	22.149552395618688	27.701524587726173	28.024308142212284	22.12461487444286
84-85	22.043275766619843	27.56745099970832	27.957295372629797	22.43197786104204
86-87	21.932083271033108	27.833190061191686	27.967743846759234	22.266982821015972
88-89	22.22249389661592	27.554417660982562	27.892127099025316	22.330961343376202
90-91	22.29408913599344	27.758026490769343	27.980196512982587	21.967687860254628
92-93	22.102209429493705	27.721773019814922	27.918280734808874	22.2577368158825
94-95	22.200227192097504	27.55247702174195	27.9922620636577	22.255033722502844
96-97	22.171265111063352	27.83658235697003	28.006640778225083	21.985511753741534
98-99	22.41636363165698	28.281199339115105	28.171165110558434	21.13127191866948
100-101	22.81128556651048	30.80118747576624	25.614979359923502	20.772547597799782
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2379.0
1	2183.0
2	2587.0
3	3396.0
4	4129.5
5	5674.5
6	7420.0
7	10774.0
8	14346.0
9	15348.0
10	14299.5
11	12966.5
12	13609.0
13	16935.5
14	24555.0
15	34157.0
16	40852.0
17	43574.5
18	42806.0
19	39267.5
20	35185.0
21	31988.0
22	31290.5
23	34835.0
24	42653.0
25	54815.0
26	70450.0
27	87622.5
28	105880.0
29	126754.5
30	146362.0
31	165721.5
32	187311.0
33	207916.0
34	227616.0
35	245908.5
36	264307.5
37	282575.5
38	297029.0
39	305246.5
40	313850.5
41	325002.0
42	332269.0
43	338060.5
44	344741.5
45	350561.5
46	353056.0
47	353923.5
48	352241.5
49	344901.0
50	335100.5
51	324284.0
52	311003.5
53	295827.0
54	278174.5
55	257128.5
56	235246.5
57	211823.5
58	187684.5
59	161829.5
60	133475.0
61	107354.5
62	83583.5
63	63282.5
64	47724.0
65	35708.0
66	26447.5
67	19484.0
68	14398.0
69	10380.0
70	7277.0
71	5014.5
72	3425.0
73	2214.5
74	1410.0
75	894.0
76	554.0
77	337.0
78	219.5
79	150.0
80	104.5
81	78.0
82	61.5
83	49.0
84	35.5
85	23.0
86	16.0
87	10.5
88	6.0
89	4.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.004411486003766586
4	0.0
5	0.05070267913662396
6	1.1763962676710895E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.588134961355971E-4
18-19	0.0
20-21	0.0
22-23	1.1764933568420236E-5
24-25	0.0
26-27	2.5889632496666707E-4
28-29	1.353873039319593E-4
30-31	1.1784863544832744E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.869093156019662E-5
44-45	6.101149865411685E-6
46-47	8.496960366359377E-4
48-49	0.0016091598098234051
50-51	0.0023935673941482415
52-53	0.0055554952307336055
54-55	0.007271916405506976
56-57	0.005993384993409198
58-59	0.008175385044826856
60-61	0.00749363608141693
62-63	0.006557259157199338
64-65	0.0063156946792777505
66-67	0.001363622969291011
68-69	0.002296609847957041
70-71	4.1369063725855217E-4
72-73	0.0018078727104216604
74-75	0.0022531510594025997
76-77	6.140339982251626E-4
78-79	0.0
80-81	1.6385910710037846E-4
82-83	0.0
84-85	0.0
86-87	5.88084381287449E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	7.164704539140781E-4
96-97	2.3436932386793757E-5
98-99	6.148029669744026E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	108.0
20-21	433.0
22-23	783.0
24-25	1160.0
26-27	2639.0
28-29	6237.0
30-31	15736.0
32-33	20216.0
34-35	27881.0
36-37	44615.0
38-39	52181.0
40-41	53952.0
42-43	61592.0
44-45	73607.0
46-47	73961.0
48-49	69818.0
50-51	62229.0
52-53	53670.0
54-55	56329.0
56-57	58418.0
58-59	34938.0
60-61	66061.0
62-63	63657.0
64-65	66180.0
66-67	70612.0
68-69	72019.0
70-71	73223.0
72-73	67311.0
74-75	67852.0
76-77	71062.0
78-79	74804.0
80-81	75550.0
82-83	71951.0
84-85	70087.0
86-87	72762.0
88-89	77280.0
90-91	79687.0
92-93	79422.0
94-95	84636.0
96-97	133759.0
98-99	735544.0
100-101	5556575.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.52204978314084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.80797855821282	72.46094377937803
2	8.248388206495395	13.613478044141697
3	2.1821386975666615	5.402236747029425
4	0.8185140402223439	2.701818255017121
5	0.3715823161887908	1.533186719753309
6	0.1965769999677687	0.9733162186536413
7	0.1144233317470219	0.6609713515146409
8	0.07454953966407654	0.49215846587753265
9	0.050590100947995535	0.3757318946068147
>10	0.13298438566551418	1.5789505391672356
>50	0.0016628074523327113	0.08948583986427402
>100	5.385328403044587E-4	0.0732002667397873
>500	7.248302894173806E-5	0.04452187825649212
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1763962676710895E-5	0.0
2	0.0	0.0	0.0	2.352792535342179E-5	0.0
3	0.0	0.0	0.0	2.352792535342179E-5	0.0
4	0.0	3.529188803013268E-5	0.0	3.529188803013268E-5	0.0
5	0.0	4.705585070684358E-5	0.0	4.705585070684358E-5	0.0
6	3.529188803013268E-5	4.705585070684358E-5	0.0	4.705585070684358E-5	0.0
7	3.529188803013268E-5	4.705585070684358E-5	0.0	4.705585070684358E-5	0.0
8	3.529188803013268E-5	4.705585070684358E-5	0.0	4.705585070684358E-5	0.0
9	3.529188803013268E-5	7.058377606026537E-5	0.0	7.058377606026537E-5	0.0
10-11	4.1173869368488135E-5	7.058377606026537E-5	0.0	1.2940358944381984E-4	0.0
12-13	4.705585070684358E-5	7.058377606026537E-5	0.0	1.4116755212053073E-4	0.0
14-15	4.705585070684358E-5	9.999368275204261E-5	0.0	1.4116755212053073E-4	0.0
16-17	8.234773873697626E-5	1.0587566409039805E-4	0.0	1.5881349613559707E-4	0.0
18-19	8.234773873697626E-5	1.2940358944381984E-4	0.0	2.2939727219586245E-4	0.0
20-21	8.234773873697626E-5	1.5293151479724162E-4	0.0	2.588071788876397E-4	5.881981338355448E-6
22-23	8.234773873697626E-5	1.8822340282737433E-4	0.0	2.76453122902706E-4	2.352792535342179E-5
24-25	8.234773873697626E-5	2.0586934684244066E-4	0.0	3.117450109328387E-4	4.705585070684358E-5
26-27	8.822972007533172E-5	2.117513281807961E-4	0.0	3.9997473100817043E-4	5.293783204519903E-5
28-29	1.1763962676710895E-4	2.117513281807961E-4	0.0	7.587755926478527E-4	5.881981338355447E-5
30-31	1.1763962676710895E-4	2.352792535342179E-4	0.0	0.002405730367387378	6.470179472190992E-5
32-33	1.235216081054644E-4	2.470432162109288E-4	0.0	0.005752577748911627	8.234773873697626E-5
34-35	1.2940358944381984E-4	2.76453122902706E-4	0.0	0.009928784499143996	8.234773873697626E-5
36-37	1.2940358944381984E-4	3.0586302959448324E-4	0.0	0.01609898292307886	8.234773873697626E-5
38-39	1.2940358944381984E-4	3.352729362862605E-4	0.0	0.029156981494227956	9.411170141368716E-5
40-41	1.2940358944381984E-4	3.5291888030132687E-4	0.0	0.07098375079127353	9.411170141368716E-5
42-43	1.2940358944381984E-4	3.5291888030132687E-4	0.0	0.08394763766100895	9.411170141368716E-5
44-45	1.2940358944381984E-4	3.9997473100817043E-4	0.0	0.10224059962329438	9.411170141368716E-5
46-47	1.2940358944381984E-4	4.47030581715014E-4	0.0	0.12326280092657677	9.411170141368716E-5
48-49	1.3528557078217528E-4	4.47030581715014E-4	0.0	0.14254981773504427	9.411170141368716E-5
50-51	1.5881349613559707E-4	5.176143577752795E-4	0.0	0.16207799577838436	9.411170141368716E-5
52-53	1.6469547747395251E-4	5.293783204519903E-4	0.0	0.1961170217834473	1.117576454287535E-4
54-55	1.6469547747395251E-4	5.293783204519903E-4	0.0	0.27734718406613607	1.2940358944381984E-4
56-57	1.6469547747395251E-4	5.411422831287011E-4	0.0	0.3658180653763403	1.3528557078217528E-4
58-59	1.6469547747395251E-4	5.411422831287011E-4	0.0	0.4549653745404555	1.5293151479724162E-4
60-61	1.6469547747395251E-4	5.470242644670567E-4	0.0	0.8318768567209343	1.6469547747395251E-4
62-63	2.117513281807961E-4	5.587882271437675E-4	0.0	0.8407233566538208	1.9998736550408522E-4
64-65	2.117513281807961E-4	6.528999285574546E-4	0.0	0.8491816458183759	1.9998736550408522E-4
66-67	2.23515290857507E-4	6.70545872572521E-4	0.0	0.8564576567339216	1.9998736550408522E-4
68-69	2.23515290857507E-4	6.881918165875874E-4	0.0	0.863563090190655	1.9998736550408522E-4
70-71	2.2939727219586245E-4	7.117197419410092E-4	0.0	0.8702038471216583	1.9998736550408522E-4
72-73	3.1762699227119414E-4	7.176017232793646E-4	0.0	0.8746565069947934	1.9998736550408522E-4
74-75	3.1762699227119414E-4	7.587755926478527E-4	0.0	0.8783974471259874	1.9998736550408522E-4
76-77	3.293909549479051E-4	7.999494620163409E-4	0.0	0.881650182806098	1.9998736550408522E-4
78-79	3.41154917624616E-4	8.117134246930517E-4	0.0	0.8847205770647195	1.9998736550408522E-4
80-81	3.41154917624616E-4	8.117134246930517E-4	0.0	0.8861969543806467	2.0586934684244066E-4
82-83	3.41154917624616E-4	8.117134246930517E-4	0.0	0.8866851588317304	2.352792535342179E-4
84-85	3.588008616396823E-4	8.175954060314072E-4	0.0	0.8872204191335207	2.352792535342179E-4
86-87	3.7056482431639323E-4	8.470053127231844E-4	0.0	0.8874556983870548	2.352792535342179E-4
88-89	3.7644680565474865E-4	8.705332380766062E-4	0.0	0.8875439281071301	2.352792535342179E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12295	0.0	54.09052	1
GTATCAA	23300	0.0	38.945305	1
ATCAACG	29975	0.0	30.100088	3
TCAACGC	30090	0.0	30.041676	4
TATCAAC	30975	0.0	29.337189	2
CAACGCA	30805	0.0	29.330395	5
AACGCAG	31710	0.0	28.533266	6
GTGGTAT	5505	0.0	24.913519	1
ACGCAGA	36330	0.0	24.892893	7
CGCAGAG	36865	0.0	24.484867	8
TGGTATC	5480	0.0	24.161457	2
GCAGAGT	42175	0.0	21.289698	9
CGAGACC	8940	0.0	21.066902	88-89
GAGACCG	9215	0.0	21.019537	88-89
ACGAGAC	9130	0.0	20.93467	86-87
GACCGAG	9325	0.0	20.85252	90-91
ACCGAGG	9495	0.0	20.680546	92-93
AGACCGA	9240	0.0	20.549185	90-91
CACGAGA	9395	0.0	19.84002	86-87
GAGTACT	23955	0.0	19.79392	12-13
>>END_MODULE