FastQCFastQC Report
Fri 27 May 2016
SRR2140385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978489
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86150.051.1519281
GTATCAA164950.039.808891
TCAACGC197100.033.180144
ATCAACG196000.032.7900853
CAACGCA202300.032.2409975
TATCAAC203850.031.7628212
AACGCAG207450.031.46456
TGGTATC38850.031.10892
GTGGTAT43900.029.8162211
ACGCAGA238950.027.2801447
CGCAGAG243250.026.779988
GCAGAGT279850.023.2619959
GAGTACT169950.021.52550112-13
GAGACCG51350.020.13099988-89
CGAGACC50750.020.09668788-89
GACCGAG53250.019.65497890-91
CAGAGTA276000.019.64487310-11
AGACCGA52300.019.58393590-91
AGAGTAC256300.019.36003710-11
ACCGAGG54950.019.25422192-93
Produced by FastQC (version 0.11.5)