##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5978489
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.829764510731724	32.0	30.0	33.0	18.0	33.0
2	30.954649577844837	33.0	31.0	33.0	27.0	33.0
3	30.073817481306733	32.0	29.0	33.0	18.0	33.0
4	30.51757408937275	33.0	31.0	33.0	27.0	33.0
5	30.68391494907827	33.0	31.0	33.0	25.0	33.0
6	33.9554660048718	37.0	33.0	38.0	26.0	38.0
7	35.46568338588563	38.0	36.0	38.0	30.0	38.0
8	35.490904808890676	38.0	36.0	38.0	29.0	38.0
9	36.054771364470184	38.0	37.0	38.0	31.0	38.0
10-11	36.22326360389724	38.0	37.0	38.0	33.0	38.0
12-13	36.3307294702725	38.0	37.0	38.0	33.0	38.0
14-15	36.3706504268888	38.0	37.0	38.0	33.0	38.0
16-17	36.420224073340265	38.0	37.5	38.0	33.5	38.0
18-19	36.39533617942594	38.0	38.0	38.0	33.5	38.0
20-21	36.4508361033211	38.0	38.0	38.0	34.0	38.0
22-23	36.49839739300186	38.0	38.0	38.0	34.0	38.0
24-25	36.50771107235076	38.0	38.0	38.0	34.0	38.0
26-27	36.4630428934675	38.0	38.0	38.0	34.0	38.0
28-29	36.44817990112274	38.0	38.0	38.0	34.0	38.0
30-31	36.43580797506826	38.0	38.0	38.0	34.0	38.0
32-33	36.41415812490954	38.0	38.0	38.0	34.0	38.0
34-35	36.39182684080897	38.0	38.0	38.0	34.0	38.0
36-37	36.355379324718825	38.0	38.0	38.0	33.0	38.0
38-39	36.35009167473535	38.0	38.0	38.0	33.0	38.0
40-41	36.326540022383185	38.0	38.0	38.0	33.0	38.0
42-43	36.28816836162572	38.0	37.0	38.0	33.0	38.0
44-45	36.23252685189899	38.0	37.0	38.0	33.0	38.0
46-47	36.18703244387599	38.0	37.0	38.0	33.0	38.0
48-49	36.14384835129769	38.0	37.0	38.0	33.0	38.0
50-51	36.12339373045896	38.0	37.0	38.0	33.0	38.0
52-53	36.09423863192983	38.0	37.0	38.0	33.0	38.0
54-55	36.01023363672182	38.0	37.0	38.0	31.0	38.0
56-57	36.03846846238703	38.0	37.0	38.0	31.5	38.0
58-59	36.023970458435485	38.0	37.0	38.0	31.5	38.0
60-61	36.020380246749156	38.0	37.0	38.0	31.0	38.0
62-63	36.00342385601979	38.0	37.0	38.0	31.0	38.0
64-65	36.00687035130218	38.0	37.0	38.0	31.0	38.0
66-67	36.00133390790684	38.0	37.0	38.0	31.0	38.0
68-69	35.98506968575441	38.0	37.0	38.0	31.0	38.0
70-71	35.97036804198086	38.0	37.0	38.0	31.0	38.0
72-73	35.95673459391098	38.0	37.0	38.0	31.0	38.0
74-75	35.940386303481745	38.0	37.0	38.0	31.0	38.0
76-77	35.945719521119614	38.0	37.0	38.0	31.0	38.0
78-79	35.91842300378374	38.0	37.0	38.0	31.0	38.0
80-81	35.906060214232895	38.0	37.0	38.0	31.0	38.0
82-83	35.81653842419904	38.0	37.0	38.0	31.0	38.0
84-85	35.838054225402246	38.0	37.0	38.0	31.0	38.0
86-87	35.84495957451475	38.0	37.0	38.0	31.0	38.0
88-89	35.85268474594905	38.0	37.0	38.0	31.0	38.0
90-91	35.829384020927336	38.0	37.0	38.0	31.0	38.0
92-93	35.79857889466561	38.0	37.0	38.0	31.0	38.0
94-95	35.8057662141517	38.0	37.0	38.0	31.0	38.0
96-97	35.82006512054386	38.0	37.0	38.0	31.0	38.0
98-99	35.82444451980108	38.0	37.0	38.0	31.0	38.0
100-101	35.2346654690552	38.0	35.5	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	65.0
21	439.0
22	1942.0
23	5844.0
24	12958.0
25	24255.0
26	38855.0
27	57080.0
28	79485.0
29	106322.0
30	134468.0
31	169790.0
32	212643.0
33	272177.0
34	367867.0
35	547355.0
36	1068958.0
37	2877978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.348221766402844	20.242322098443267	14.785408152461265	24.624047982692616
2	13.872702617668109	20.797161289416106	43.00479602789267	22.32534006502312
3	18.111260672262414	25.625256033097415	30.42187563511681	25.84160765952336
4	12.083454531738704	18.3351512397196	40.560850743390176	29.020543485151517
5	13.459451363140767	36.93325333808619	35.65508660154919	13.952208697223847
6	28.457730449571866	39.07763969752887	19.155696222857685	13.308933630041576
7	25.429100898236996	33.60004509500645	23.36352881137692	17.607325195379637
8	23.467267398166992	37.42676452193857	21.374865789666924	17.73110229022751
9	24.773416828232016	17.788725545869532	21.946515248250854	35.4913423776476
10-11	23.336164037434877	27.578172344216075	29.125595112745046	19.96006850560401
12-13	24.37738866793934	25.07572565576352	29.46526287829584	21.081622798001302
14-15	21.59954630676748	26.53864546710716	27.296612906706024	24.565195319419338
16-17	20.82618477837431	29.874821045680335	27.91893961024548	21.380054565699883
18-19	20.80622712528199	28.723704267081533	30.122184719249294	20.347883888387184
20-21	21.59493534529623	27.727840379340602	30.17647440591706	20.500749869446107
22-23	21.356769356368893	27.602643352421648	30.06199053025377	20.978596760955693
24-25	21.106270116460184	27.759236601901133	30.13856962646736	20.99592365517132
26-27	21.0249641583043	27.90628529111354	30.074681737370828	20.994068813211328
28-29	20.923141968653823	27.884082189850247	30.180086765288998	21.012689076206932
30-31	21.22730908328533	27.82066937811679	30.050022812265233	20.90199872633264
32-33	21.024841664185363	27.955885031532084	30.028687498893586	20.990585805388967
34-35	21.149262745642833	27.959659435596052	30.003143407497458	20.887934411263657
36-37	21.141423037305803	27.849560003547413	29.976176474832144	21.032840484314637
38-39	20.98790107675727	28.0021762916665	30.03418178802407	20.975740843552153
40-41	21.207007454771766	27.90635427785447	29.92205111838625	20.96458714898752
42-43	21.129071262737277	27.93322640052278	29.79774405545213	21.139958281287814
44-45	21.179928287693023	28.06192406560549	29.604760611710706	21.15338703499078
46-47	21.26326054742265	28.043940753262348	29.35235217964015	21.340446519674853
48-49	21.17396385354613	28.183499273168177	29.34663190736896	21.29590496591674
50-51	21.170520065253086	28.347741063346838	29.187000833420353	21.294738037979727
52-53	21.355501893451887	28.29200601515846	29.043998627535238	21.30849346385441
54-55	21.33461232302092	28.23020025579794	29.053914334522883	21.381273086658258
56-57	21.264394353646434	28.270157270704082	29.095543809195913	21.369904566453567
58-59	21.21995967816839	28.301135885333693	29.135527223488765	21.343377213009152
60-61	21.239537590430377	28.169466832915163	29.202451219094993	21.388544357559468
62-63	21.32663436512401	28.22123202284974	29.23167714148028	21.22045647054597
64-65	21.137807297266633	28.209243563816074	29.157956354725105	21.49499278419219
66-67	21.21908824325422	28.30702522101075	28.975223332014384	21.498663203720643
68-69	21.173238160779132	28.469785012668254	29.007738868427978	21.34923795812464
70-71	21.16958434489565	28.2232511250062	29.110375345300337	21.496789184797812
72-73	21.22883689412124	28.276209610071056	28.819972226331537	21.674981269476167
74-75	21.122738022919997	28.380633860610853	28.80578732090206	21.69084079556709
76-77	21.30471155649369	28.126713870743696	28.907993961256928	21.660580611505686
78-79	21.336112757372298	28.16332268435662	28.760107864207633	21.740456694063454
80-81	21.484311189611795	28.194321011640273	28.590552254150104	21.73081554459783
82-83	21.494536931603353	28.369297876844573	28.671526939638188	21.464638251913883
84-85	21.40172594580316	28.2110617252532	28.62688411656315	21.760328212380486
86-87	21.29910447397306	28.422475361732886	28.64875682336383	21.62966334093023
88-89	21.549186692886167	28.24008280273725	28.60280638910516	21.60792411527142
90-91	21.61110484031708	28.39376150314975	28.649734278989804	21.345399377543366
92-93	21.460957194675863	28.372292967170644	28.597123150132898	21.569626688020595
94-95	21.518689277247105	28.3054224845016	28.625516833897315	21.550371404353978
96-97	21.483185233250925	28.49735886696734	28.692582840411212	21.326873059370524
98-99	21.748971405419653	28.892259834784507	28.807406390608797	20.551362369187043
100-101	22.117290512861878	31.502764931532028	26.379606088358727	20.000338467247364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1644.0
1	1451.0
2	1653.5
3	2192.0
4	2608.5
5	3512.5
6	4652.0
7	6560.0
8	9052.0
9	10371.0
10	9864.5
11	9112.0
12	9666.0
13	11708.5
14	16216.5
15	22039.0
16	26865.0
17	29778.5
18	30044.5
19	28195.5
20	25932.5
21	24546.0
22	24738.5
23	27852.5
24	34224.0
25	43756.0
26	56610.0
27	71001.0
28	86342.5
29	102024.5
30	117182.5
31	132375.5
32	148336.5
33	164103.5
34	178531.0
35	191520.5
36	205552.5
37	216900.0
38	224340.0
39	231281.5
40	238024.5
41	243401.5
42	245079.5
43	245956.0
44	246918.0
45	245968.5
46	242764.0
47	238078.0
48	231691.0
49	223570.5
50	214798.5
51	204454.0
52	191950.0
53	178051.0
54	164178.5
55	150601.0
56	136459.5
57	122493.0
58	108493.5
59	92953.5
60	76634.0
61	61295.0
62	47255.0
63	35640.5
64	26492.0
65	19375.0
66	14036.0
67	10394.5
68	7771.5
69	5672.5
70	4065.0
71	2853.5
72	1970.5
73	1277.0
74	816.5
75	525.5
76	327.0
77	195.0
78	130.0
79	81.5
80	46.0
81	29.0
82	21.0
83	17.0
84	12.5
85	6.0
86	2.5
87	3.5
88	4.5
89	3.0
90	1.5
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.004315471685236855
4	0.0
5	0.05242127233152056
6	1.672663443890254E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.338130755112203E-4
18-19	0.0
20-21	8.363477396740537E-6
22-23	0.0
24-25	0.0
26-27	2.1751346471090828E-4
28-29	1.7574635081532499E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.429692214846107E-5
44-45	0.0
46-47	0.001131108431447564
48-49	0.0016312339556958614
50-51	0.002888706614740619
52-53	0.006063515233159497
54-55	0.007583923084710633
56-57	0.006318332154721374
58-59	0.008363058585264056
60-61	0.007678576952259994
62-63	0.006781727533282243
64-65	0.006849309378227634
66-67	0.0014978667028883708
68-69	0.002364246513228944
70-71	4.826704737694624E-4
72-73	0.0020137890146568528
74-75	0.0027034372617355394
76-77	7.179226578040085E-4
78-79	0.0
80-81	2.1741035997917998E-4
82-83	0.0
84-85	1.0066875259033284E-5
86-87	4.0634140459423814E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	9.32130122822973E-4
96-97	4.290163491695423E-5
98-99	6.979670546255607E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	67.0
20-21	221.0
22-23	509.0
24-25	760.0
26-27	1644.0
28-29	3774.0
30-31	9163.0
32-33	12593.0
34-35	17799.0
36-37	27567.0
38-39	30890.0
40-41	32246.0
42-43	37329.0
44-45	45473.0
46-47	46442.0
48-49	42383.0
50-51	37779.0
52-53	32553.0
54-55	35042.0
56-57	35884.0
58-59	22092.0
60-61	40663.0
62-63	38924.0
64-65	41384.0
66-67	44689.0
68-69	45797.0
70-71	45475.0
72-73	41805.0
74-75	42582.0
76-77	45544.0
78-79	47674.0
80-81	48306.0
82-83	45554.0
84-85	44448.0
86-87	46869.0
88-89	49805.0
90-91	51795.0
92-93	51565.0
94-95	54574.0
96-97	90070.0
98-99	518954.0
100-101	4069802.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.69867441609719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.66318959619082	81.30403409933515
2	6.252274088749911	11.09136847516458
3	1.256885275253104	3.344521734241853
4	0.41835838298196143	1.4843133600544756
5	0.1704574235384066	0.7559673756119953
6	0.07857325426280773	0.41816060986020015
7	0.04993403992628398	0.3100358204791302
8	0.029613705129981675	0.21013571116628413
9	0.021162344774872447	0.1689364736160846
>10	0.058297031905552424	0.7804686356270337
>50	8.482002006646691E-4	0.0529340104558087
>100	3.546684987738625E-4	0.050971594355423026
>500	5.198858683802153E-5	0.02815210003193665
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.672663443890254E-5	0.0	0.0	0.0
3	0.0	1.672663443890254E-5	0.0	0.0	0.0
4	0.0	5.0179903316707617E-5	0.0	3.345326887780508E-5	0.0
5	0.0	5.0179903316707617E-5	0.0	3.345326887780508E-5	0.0
6	1.672663443890254E-5	5.0179903316707617E-5	0.0	3.345326887780508E-5	0.0
7	1.672663443890254E-5	5.0179903316707617E-5	0.0	5.0179903316707617E-5	0.0
8	1.672663443890254E-5	8.36331721945127E-5	0.0	5.0179903316707617E-5	1.672663443890254E-5
9	1.672663443890254E-5	1.338130755112203E-4	0.0	6.690653775561016E-5	3.345326887780508E-5
10-11	1.672663443890254E-5	1.338130755112203E-4	0.0	7.526985497506142E-5	3.345326887780508E-5
12-13	1.672663443890254E-5	1.338130755112203E-4	0.0	9.199648941396396E-5	3.345326887780508E-5
14-15	1.672663443890254E-5	1.7562966160847667E-4	0.0	1.087231238528665E-4	4.181658609725635E-5
16-17	6.690653775561016E-5	1.8399297882792793E-4	0.0	1.1708644107231777E-4	5.0179903316707617E-5
18-19	6.690653775561016E-5	2.0908293048628175E-4	0.0	1.421763927306716E-4	5.0179903316707617E-5
20-21	6.690653775561016E-5	2.3417288214463554E-4	0.0	1.8399297882792793E-4	5.0179903316707617E-5
22-23	6.690653775561016E-5	2.3417288214463554E-4	0.0	2.0071961326683047E-4	6.690653775561016E-5
24-25	6.690653775561016E-5	3.010794199002457E-4	0.0	2.0908293048628175E-4	8.36331721945127E-5
26-27	6.690653775561016E-5	3.345326887780508E-4	0.0	3.0944273711969693E-4	8.36331721945127E-5
28-29	6.690653775561016E-5	3.345326887780508E-4	0.0	6.52338743117199E-4	8.36331721945127E-5
30-31	6.690653775561016E-5	3.5962264043640457E-4	0.0	0.001798113202182023	8.36331721945127E-5
32-33	6.690653775561016E-5	3.6798595765585586E-4	0.0	0.00434892495411466	1.1708644107231777E-4
34-35	6.690653775561016E-5	4.181658609725635E-4	0.0	0.0077109784763340706	1.1708644107231777E-4
36-37	6.690653775561016E-5	4.683457642892711E-4	0.0	0.013063501496782884	1.1708644107231777E-4
38-39	6.690653775561016E-5	5.101623503865274E-4	0.0	0.024471066184114414	1.338130755112203E-4
40-41	6.690653775561016E-5	5.352523020448812E-4	0.0	0.06077622623375237	1.338130755112203E-4
42-43	6.690653775561016E-5	5.770688881421376E-4	0.0	0.07097110992426348	1.338130755112203E-4
44-45	6.690653775561016E-5	6.690653775561016E-4	0.0	0.08553164520332812	1.338130755112203E-4
46-47	6.690653775561016E-5	7.192452808728091E-4	0.0	0.10233354949720573	1.338130755112203E-4
48-49	8.36331721945127E-5	7.192452808728091E-4	0.0	0.11875074119898857	1.338130755112203E-4
50-51	8.36331721945127E-5	8.196050875062244E-4	0.0	0.13500902987360186	1.338130755112203E-4
52-53	8.36331721945127E-5	8.196050875062244E-4	0.0	0.1623403505467686	1.5053970995012285E-4
54-55	8.36331721945127E-5	8.196050875062244E-4	0.0	0.22988250041105707	1.672663443890254E-4
56-57	8.36331721945127E-5	8.363317219451269E-4	0.0	0.3083136892950711	1.672663443890254E-4
58-59	8.36331721945127E-5	8.363317219451269E-4	0.0	0.3867532414963045	1.672663443890254E-4
60-61	9.199648941396396E-5	8.363317219451269E-4	0.0	0.7087242278107395	1.672663443890254E-4
62-63	1.338130755112203E-4	8.530583563840295E-4	0.0	0.715757777592298	1.8399297882792793E-4
64-65	1.338130755112203E-4	9.03238259700737E-4	0.0	0.723134223379854	1.8399297882792793E-4
66-67	1.338130755112203E-4	9.199648941396396E-4	0.0	0.7295488876871732	1.8399297882792793E-4
68-69	1.338130755112203E-4	9.199648941396396E-4	0.0	0.7357042891606893	1.8399297882792793E-4
70-71	1.421763927306716E-4	9.283282113590909E-4	0.0	0.7411237187188937	2.17446247705733E-4
72-73	1.5053970995012285E-4	9.366915285785422E-4	0.0	0.7450293878603774	2.3417288214463554E-4
74-75	1.5053970995012285E-4	9.534181630174447E-4	0.0	0.748098725279916	2.3417288214463554E-4
76-77	1.5053970995012285E-4	9.95234749114701E-4	0.0	0.7505073606391179	2.3417288214463554E-4
78-79	1.5053970995012285E-4	0.0010035980663341522	0.0	0.752974539218856	2.3417288214463554E-4
80-81	1.5053970995012285E-4	0.0010119613835536034	0.0	0.7540952237262626	2.425361993640868E-4
82-83	1.5053970995012285E-4	0.0010203247007730548	0.0	0.7546806559316241	2.5089951658353806E-4
84-85	1.5053970995012285E-4	0.0010286880179925062	0.0	0.7550570052064995	2.5089951658353806E-4
86-87	1.5053970995012285E-4	0.00105377796965086	0.0	0.7552911780886442	2.5089951658353806E-4
88-89	1.5053970995012285E-4	0.0011206845074064702	0.0	0.7553580846263998	2.5089951658353806E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8615	0.0	51.151928	1
GTATCAA	16495	0.0	39.80889	1
TCAACGC	19710	0.0	33.18014	4
ATCAACG	19600	0.0	32.790085	3
CAACGCA	20230	0.0	32.240997	5
TATCAAC	20385	0.0	31.762821	2
AACGCAG	20745	0.0	31.4645	6
TGGTATC	3885	0.0	31.1089	2
GTGGTAT	4390	0.0	29.816221	1
ACGCAGA	23895	0.0	27.280144	7
CGCAGAG	24325	0.0	26.77998	8
GCAGAGT	27985	0.0	23.261995	9
GAGTACT	16995	0.0	21.525501	12-13
GAGACCG	5135	0.0	20.130999	88-89
CGAGACC	5075	0.0	20.096687	88-89
GACCGAG	5325	0.0	19.654978	90-91
CAGAGTA	27600	0.0	19.644873	10-11
AGACCGA	5230	0.0	19.583935	90-91
AGAGTAC	25630	0.0	19.360037	10-11
ACCGAGG	5495	0.0	19.254221	92-93
>>END_MODULE