FastQCFastQC Report
Fri 27 May 2016
SRR2140384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8876479
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126550.052.10221
GTATCAA214350.032.6438941
GTGGTAT59750.026.5890981
ATCAACG267300.026.144923
TCAACGC268450.026.014264
CAACGCA275700.025.4246255
TGGTATC59800.025.187712
TATCAAC283450.024.8084132
AACGCAG285800.024.5748336
ACGCAGA326300.021.4315667
CGCAGAG330500.021.1592128
GACCGAG93800.019.04356490-91
ACGAGAC93400.019.0067786-87
GAGACCG94150.018.92454388-89
GAGTACT236850.018.8747212-13
AGACCGA90750.018.81015690-91
CGAGACC91500.018.52646888-89
GCCCACG94450.018.48880482-83
ACCGAGG95650.018.3945292-93
GCAGAGT384750.018.2997789
Produced by FastQC (version 0.11.5)