##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140384_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8876479
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.513886418252103	30.0	18.0	32.0	18.0	33.0
2	30.269593833320624	32.0	29.0	33.0	25.0	33.0
3	30.854084710840866	33.0	31.0	33.0	27.0	33.0
4	31.363077972696157	33.0	31.0	33.0	28.0	34.0
5	31.87031096451645	33.0	32.0	33.0	30.0	34.0
6	35.50818055222121	38.0	36.0	38.0	29.0	38.0
7	35.963474706581295	38.0	37.0	38.0	31.0	38.0
8	36.10751605450765	38.0	37.0	38.0	33.0	38.0
9	36.36800481362035	38.0	37.0	38.0	33.0	38.0
10-11	36.420878312222676	38.0	37.5	38.0	33.5	38.0
12-13	36.457404957528766	38.0	38.0	38.0	34.0	38.0
14-15	36.43961181004315	38.0	38.0	38.0	34.0	38.0
16-17	36.45095859518172	38.0	38.0	38.0	34.0	38.0
18-19	36.39860720675394	38.0	37.5	38.0	33.5	38.0
20-21	36.42938392575429	38.0	38.0	38.0	33.5	38.0
22-23	36.4663068921807	38.0	38.0	38.0	34.0	38.0
24-25	36.469720575801716	38.0	38.0	38.0	34.0	38.0
26-27	36.414811793683334	38.0	38.0	38.0	34.0	38.0
28-29	36.39324135616221	38.0	38.0	38.0	33.5	38.0
30-31	36.38312951023481	38.0	38.0	38.0	33.5	38.0
32-33	36.36105819419106	38.0	38.0	38.0	33.0	38.0
34-35	36.33888920043408	38.0	37.5	38.0	33.0	38.0
36-37	36.30120136501284	38.0	37.0	38.0	33.0	38.0
38-39	36.296806276214824	38.0	37.0	38.0	33.0	38.0
40-41	36.28053997934971	38.0	37.0	38.0	33.0	38.0
42-43	36.24581504393327	38.0	37.0	38.0	33.0	38.0
44-45	36.19253970081594	38.0	37.0	38.0	33.0	38.0
46-47	36.14791413726612	38.0	37.0	38.0	33.0	38.0
48-49	36.103915604534805	38.0	37.0	38.0	33.0	38.0
50-51	36.07402435982272	38.0	37.0	38.0	33.0	38.0
52-53	36.03879402141865	38.0	37.0	38.0	31.5	38.0
54-55	35.95018887380187	38.0	37.0	38.0	31.0	38.0
56-57	35.977343999247374	38.0	37.0	38.0	31.0	38.0
58-59	35.95608492510881	38.0	37.0	38.0	31.0	38.0
60-61	35.948530218155994	38.0	37.0	38.0	31.0	38.0
62-63	35.931514894854956	38.0	37.0	38.0	31.0	38.0
64-65	35.93082159789417	38.0	37.0	38.0	31.0	38.0
66-67	35.92281592574509	38.0	37.0	38.0	31.0	38.0
68-69	35.90330587367828	38.0	37.0	38.0	31.0	38.0
70-71	35.88371942730545	38.0	37.0	38.0	31.0	38.0
72-73	35.870018106238504	38.0	37.0	38.0	31.0	38.0
74-75	35.85584563047729	38.0	37.0	38.0	31.0	38.0
76-77	35.86207276155604	38.0	37.0	38.0	31.0	38.0
78-79	35.8275981647741	38.0	37.0	38.0	31.0	38.0
80-81	35.811966614678056	38.0	37.0	38.0	31.0	38.0
82-83	35.71823297160485	38.0	37.0	38.0	30.0	38.0
84-85	35.738023214324286	38.0	37.0	38.0	30.0	38.0
86-87	35.74181925926152	38.0	37.0	38.0	31.0	38.0
88-89	35.75130746972967	38.0	37.0	38.0	31.0	38.0
90-91	35.72696105413439	38.0	37.0	38.0	31.0	38.0
92-93	35.69463889406723	38.0	36.0	38.0	30.5	38.0
94-95	35.7003338633874	38.0	36.0	38.0	31.0	38.0
96-97	35.7118204976058	38.0	36.0	38.0	31.0	38.0
98-99	35.721184083291206	38.0	36.0	38.0	31.0	38.0
100-101	35.12422143395601	38.0	35.0	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	14.0
20	107.0
21	660.0
22	2915.0
23	8828.0
24	19707.0
25	36261.0
26	58420.0
27	87469.0
28	121570.0
29	159995.0
30	204312.0
31	254141.0
32	318312.0
33	406000.0
34	544906.0
35	804406.0
36	1582511.0
37	4265943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.279437263356336	20.447071411986666	17.264491922979822	23.008999401677173
2	14.092198043841483	22.572824202028755	41.02162580455606	22.313351949573697
3	19.01650728621819	25.89220923822639	30.982360464981344	24.108923010574077
4	12.768858012281672	19.51999210497766	38.67432120326089	29.036828679479783
5	13.409667731331233	37.67991715318208	36.0725960119825	12.837819103504197
6	29.006380683870336	39.17536887941366	18.72848668131662	13.089763755399384
7	25.62940778657844	33.262332958822974	23.340786363602053	17.76747289099653
8	23.629526977983048	37.789341922624956	21.10464070269304	17.476490396698964
9	24.553834915848956	17.619441222133236	21.783164247896043	36.043559614121776
10-11	23.59949817940199	27.607675295576094	29.033403898099685	19.75942262692223
12-13	24.432046760883456	24.991953453616013	29.419688820308142	21.156310965192393
14-15	21.505300694115313	26.60915437303462	27.117126058654563	24.7684188741955
16-17	20.957360463792554	29.740960608377083	27.89786275927189	21.403816168558475
18-19	21.01520208632274	28.749563875496133	29.74779752196789	20.487436516213243
20-21	21.727810137587948	27.784073866175994	29.83453451584257	20.653581480393484
22-23	21.479840116856316	27.65253948702984	29.714475474623157	21.153144921490686
24-25	21.27355349458761	27.83163624604367	29.734377764081394	21.160432495287328
26-27	21.21957166190468	27.948922950948347	29.68785014426824	21.14365524287873
28-29	21.11497208660708	27.980019127696114	29.75746764500092	21.14754114069589
30-31	21.426384876247024	27.837415931979443	29.677172031054038	21.05902716071949
32-33	21.221928138317388	27.91857301403926	29.662856486935123	21.19664236070823
34-35	21.358243225542402	27.971460867980852	29.627626352976584	21.042669553500165
36-37	21.32852909625218	27.926419160663364	29.597915424487304	21.147136318597155
38-39	21.179846944243973	28.031154206175106	29.68021779362482	21.1087810559561
40-41	21.437259892446992	27.886014251839914	29.56706387575847	21.109661979954623
42-43	21.343290150716506	27.95782438447147	29.383042162140782	21.315843302671244
44-45	21.35816968054131	28.088873210122074	29.26205648398722	21.2909006253494
46-47	21.447538226449694	28.0889956076231	29.001142371625054	21.462323794302147
48-49	21.329463090941474	28.199301292671937	29.004788974784802	21.466446641601785
50-51	21.355332404429014	28.273120790809042	28.90873559838671	21.46281120637524
52-53	21.496052405965816	28.24599436416021	28.80542541683572	21.452527813038255
54-55	21.48544783113251	28.185530649755126	28.815056211484396	21.51396530762797
56-57	21.464351319887975	28.217792538608126	28.79214909824443	21.52570704325947
58-59	21.429289513300045	28.223519806340917	28.861900196947992	21.485290483411045
60-61	21.411194702190738	28.129981134254805	28.936190659531054	21.522633504023407
62-63	21.501770754295197	28.202946242592937	28.93572889258459	21.359554110527277
64-65	21.270720427326676	28.182384250726283	28.890598972068695	21.656296349878346
66-67	21.346117015050076	28.285861499176647	28.730450662958656	21.637570822814624
68-69	21.315020537007303	28.442198172345805	28.729783773987595	21.512997516659293
70-71	21.311359394497124	28.217465170195805	28.853688757911655	21.617486677395412
72-73	21.345191466715864	28.24112895781678	28.59639163859017	21.81728793687719
74-75	21.299239000686452	28.29065969086689	28.574924535090812	21.835176773355844
76-77	21.44135553586651	28.09090422296977	28.64457439741589	21.823165843747827
78-79	21.47271145904182	28.05694473463609	28.575149606664574	21.89519419965751
80-81	21.660478492151096	28.073116739480668	28.401320194779174	21.865084573589062
82-83	21.654110112742327	28.316203668683297	28.420057816019455	21.60962840255492
84-85	21.528722950062118	28.13403026834681	28.417950559392413	21.919296222198657
86-87	21.445887360610215	28.38261307214441	28.438984234247673	21.73251533299771
88-89	21.731833034780987	28.165235442366548	28.35762753703495	21.745303985817515
90-91	21.792528963030353	28.33328478669564	28.387494017601384	21.486692232672624
92-93	21.58071293193664	28.31265109152944	28.38405239077501	21.722583585758912
94-95	21.711490346718634	28.18722041934098	28.43021594953154	21.67107328440884
96-97	21.647794806804583	28.457384476415303	28.458648553326444	21.43617216345367
98-99	21.9226701474088	28.793378116645556	28.654115997591497	20.62983573835415
100-101	22.25467280293776	31.452692565395207	26.158838057258183	20.133796574408855
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2436.0
1	2113.5
2	2180.5
3	2716.5
4	3301.5
5	4545.5
6	6052.5
7	9119.5
8	11983.0
9	12983.5
10	12643.5
11	11611.0
12	12404.5
13	15492.0
14	22482.0
15	31443.0
16	37627.0
17	40062.0
18	40191.5
19	38630.0
20	36214.0
21	34490.0
22	35400.5
23	40632.5
24	50389.0
25	64722.0
26	82543.0
27	102680.5
28	124570.5
29	147401.0
30	170194.0
31	192582.5
32	215286.5
33	238551.0
34	259724.0
35	278770.5
36	299727.0
37	317878.5
38	332139.0
39	343829.0
40	353981.0
41	363580.5
42	368523.5
43	371997.5
44	374796.5
45	374918.5
46	372629.0
47	367858.0
48	358822.5
49	346192.0
50	333249.5
51	317077.0
52	296433.5
53	274645.0
54	253058.5
55	231070.5
56	208522.0
57	185387.0
58	162161.5
59	138012.0
60	113271.0
61	90589.5
62	69976.5
63	52738.5
64	39296.5
65	28999.5
66	21327.5
67	15935.0
68	11949.0
69	8647.5
70	6172.5
71	4289.0
72	2922.0
73	1984.0
74	1305.5
75	840.0
76	545.0
77	318.0
78	209.0
79	144.5
80	85.0
81	53.0
82	35.5
83	24.0
84	17.5
85	12.5
86	9.5
87	6.0
88	3.5
89	4.0
90	4.5
91	3.0
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0038078161397103513
4	0.0
5	0.04732732426900351
6	1.1265728223995123E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.182901463519488E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.873692751532636E-4
28-29	2.6492583711209644E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.4457863655174632E-4
44-45	0.0
46-47	9.809954742625558E-4
48-49	0.001670358732046087
50-51	0.002262220617246
52-53	0.005366663297227448
54-55	0.007009424924765102
56-57	0.005699819724932546
58-59	0.007801497998014176
60-61	0.007373267574709926
62-63	0.005966211500397271
64-65	0.005937938345303691
66-67	0.0013251740474128344
68-69	0.00223507129972962
70-71	4.3708403499783294E-4
72-73	0.001874650611181506
74-75	0.00222465465671583
76-77	6.272459531741749E-4
78-79	0.0
80-81	2.1550195827302926E-4
82-83	0.0
84-85	0.0
86-87	7.629904146901458E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	8.195030707432762E-4
96-97	5.879424036922783E-5
98-99	4.8650285254106144E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	383.0
22-23	759.0
24-25	1212.0
26-27	2493.0
28-29	5580.0
30-31	14742.0
32-33	19675.0
34-35	27905.0
36-37	43834.0
38-39	49122.0
40-41	50952.0
42-43	59335.0
44-45	70369.0
46-47	71723.0
48-49	66513.0
50-51	59193.0
52-53	51674.0
54-55	55702.0
56-57	56888.0
58-59	34709.0
60-61	64510.0
62-63	62017.0
64-65	66315.0
66-67	70757.0
68-69	72236.0
70-71	72805.0
72-73	66717.0
74-75	67793.0
76-77	71930.0
78-79	75137.0
80-81	75055.0
82-83	71060.0
84-85	70801.0
86-87	73377.0
88-89	77962.0
90-91	80698.0
92-93	79592.0
94-95	85378.0
96-97	137373.0
98-99	770992.0
100-101	5921125.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.35080346386368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.11491566048484	77.81495371361177
2	7.117775560496074	12.292512770485773
3	1.6350657142294789	4.235677144197949
4	0.5375409922436156	1.8566838629999491
5	0.23683879846889425	1.0225610269602552
6	0.12954342305980115	0.6711707198803821
7	0.07666259003575253	0.46339133716456155
8	0.043514874315345876	0.3006035487807329
9	0.02887469883915171	0.22440181000840578
>10	0.07829536853431807	1.0154863439317352
>50	6.131847855506246E-4	0.03828524434274277
>100	3.203830158852021E-4	0.04600211394782661
>500	3.8751491315036095E-5	0.01827036368797149
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1265728223995123E-5	0.0
2	0.0	1.1265728223995123E-5	0.0	2.2531456447990246E-5	0.0
3	0.0	1.1265728223995123E-5	0.0	3.379718467198537E-5	0.0
4	0.0	4.506291289598049E-5	0.0	4.506291289598049E-5	0.0
5	0.0	4.506291289598049E-5	0.0	4.506291289598049E-5	0.0
6	0.0	4.506291289598049E-5	0.0	4.506291289598049E-5	1.1265728223995123E-5
7	0.0	4.506291289598049E-5	0.0	4.506291289598049E-5	2.2531456447990246E-5
8	0.0	6.759436934397074E-5	0.0	4.506291289598049E-5	2.2531456447990246E-5
9	0.0	1.915173798079171E-4	0.0	6.759436934397074E-5	4.506291289598049E-5
10-11	1.1265728223995123E-5	1.915173798079171E-4	0.0	1.013915540159561E-4	4.506291289598049E-5
12-13	1.1265728223995123E-5	1.915173798079171E-4	0.0	1.1829014635194878E-4	5.632864111997561E-5
14-15	1.1265728223995123E-5	2.478460209278927E-4	0.0	1.5208733102393414E-4	6.196150523197317E-5
16-17	1.1265728223995123E-5	2.478460209278927E-4	0.0	1.6898592335992684E-4	7.32272334559683E-5
18-19	1.6898592335992684E-5	2.6474461326388534E-4	0.0	2.0841597214390974E-4	7.886009756796586E-5
20-21	2.8164320559987805E-5	2.8164320559987804E-4	0.0	2.2531456447990244E-4	9.575868990395855E-5
22-23	3.379718467198537E-5	3.267061184958586E-4	0.0	2.703774773758829E-4	1.4082160279993905E-4
24-25	3.379718467198537E-5	3.492375749438488E-4	0.0	3.041746620478683E-4	1.6898592335992684E-4
26-27	4.506291289598049E-5	3.492375749438488E-4	0.0	3.943004878398293E-4	1.6898592335992684E-4
28-29	4.506291289598049E-5	3.5487043905584634E-4	0.0	8.449296167996342E-4	1.746187874719244E-4
30-31	4.506291289598049E-5	3.830347596158342E-4	0.0	0.0020334639444311194	1.8025165158392197E-4
32-33	5.632864111997561E-5	3.943004878398293E-4	0.0	0.0049456546903338585	1.8025165158392197E-4
34-35	5.632864111997561E-5	4.506291289598049E-4	0.0	0.008584484906684283	1.8025165158392197E-4
36-37	5.632864111997561E-5	5.069577700797805E-4	0.0	0.014684876739977641	1.8025165158392197E-4
38-39	5.632864111997561E-5	5.182234983037756E-4	0.0	0.02720110079683622	1.915173798079171E-4
40-41	7.32272334559683E-5	5.632864111997561E-4	0.0	0.0660340659849474	1.915173798079171E-4
42-43	7.886009756796586E-5	5.689192753117536E-4	0.0	0.07782365057135832	1.915173798079171E-4
44-45	7.886009756796586E-5	6.308807805437269E-4	0.0	0.09495882320005489	1.915173798079171E-4
46-47	9.012582579196098E-5	6.872094216637025E-4	0.0	0.1143302428812145	1.9715024391991464E-4
48-49	9.012582579196098E-5	6.872094216637025E-4	0.0	0.1327666071197825	2.027831080319122E-4
50-51	9.012582579196098E-5	7.773352474556635E-4	0.0	0.15110157980433458	2.027831080319122E-4
52-53	9.012582579196098E-5	7.886009756796586E-4	0.0	0.182476632908161	2.2531456447990244E-4
54-55	9.012582579196098E-5	7.942338397916561E-4	0.0	0.2611790102809909	2.478460209278927E-4
56-57	9.012582579196098E-5	8.111324321276488E-4	0.0	0.3489390331459129	2.5347888503989024E-4
58-59	9.575868990395855E-5	8.22398160351644E-4	0.0	0.43451350473537986	2.591117491518878E-4
60-61	1.013915540159561E-4	8.505624809116317E-4	0.0	0.7907921598192256	2.591117491518878E-4
62-63	1.013915540159561E-4	8.787268014716196E-4	0.0	0.7983064005446303	2.591117491518878E-4
64-65	1.013915540159561E-4	9.801183554875757E-4	0.0	0.8056685539390112	2.591117491518878E-4
66-67	1.013915540159561E-4	0.0010026498119355659	0.0	0.8119154002392164	2.591117491518878E-4
68-69	1.0702441812795366E-4	0.0010082826760475635	0.0	0.817615858720558	2.591117491518878E-4
70-71	1.1265728223995122E-4	0.001025181268383556	0.0	0.8231416984144276	2.6474461326388534E-4
72-73	1.1265728223995122E-4	0.0010420798607195488	0.0	0.8270565389722659	3.1544039027186344E-4
74-75	1.1265728223995122E-4	0.001053345588943544	0.0	0.8301039184568566	3.1544039027186344E-4
76-77	1.1265728223995122E-4	0.0010871427736155294	0.0	0.8330330077950954	3.1544039027186344E-4
78-79	1.1829014635194878E-4	0.001104041365951522	0.0	0.8358550727152061	3.1544039027186344E-4
80-81	1.2392301046394635E-4	0.001104041365951522	0.0	0.8369591140811576	3.267061184958586E-4
82-83	1.2392301046394635E-4	0.001104041365951522	0.0	0.8374041103460055	3.5487043905584634E-4
84-85	1.2392301046394635E-4	0.001115307094175517	0.0	0.8378491066108533	3.6050330316784394E-4
86-87	1.2392301046394635E-4	0.001115307094175517	0.0	0.8380518897188851	3.6050330316784394E-4
88-89	1.2392301046394635E-4	0.0011603700070714977	0.0	0.8381363826805651	3.6050330316784394E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12655	0.0	52.1022	1
GTATCAA	21435	0.0	32.643894	1
GTGGTAT	5975	0.0	26.589098	1
ATCAACG	26730	0.0	26.14492	3
TCAACGC	26845	0.0	26.01426	4
CAACGCA	27570	0.0	25.424625	5
TGGTATC	5980	0.0	25.18771	2
TATCAAC	28345	0.0	24.808413	2
AACGCAG	28580	0.0	24.574833	6
ACGCAGA	32630	0.0	21.431566	7
CGCAGAG	33050	0.0	21.159212	8
GACCGAG	9380	0.0	19.043564	90-91
ACGAGAC	9340	0.0	19.00677	86-87
GAGACCG	9415	0.0	18.924543	88-89
GAGTACT	23685	0.0	18.87472	12-13
AGACCGA	9075	0.0	18.810156	90-91
CGAGACC	9150	0.0	18.526468	88-89
GCCCACG	9445	0.0	18.488804	82-83
ACCGAGG	9565	0.0	18.39452	92-93
GCAGAGT	38475	0.0	18.299778	9
>>END_MODULE