FastQCFastQC Report
Fri 27 May 2016
SRR2140383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12132228
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222500.059.000351
GTATCAA448300.041.789921
ATCAACG566050.033.0583423
TCAACGC570800.032.7971274
TATCAAC578800.032.4954532
CAACGCA578850.032.3184745
AACGCAG590550.031.7749086
GTGGTAT87500.028.9321251
TGGTATC86050.028.863612
ACGCAGA680200.027.548647
CGCAGAG694200.026.980548
TATCTCG179800.026.6515794-95
GCAGAGT777300.024.0177829
GTACATG637000.022.6160261
TACATGG637750.022.459842
GAGTACT387550.022.32475312-13
ACATGGG634800.021.9475173
CAGAGTA758550.020.86308310-11
CTATCTC246550.020.4010394-95
CATGGGG427650.020.0061444
Produced by FastQC (version 0.11.5)