##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140383_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12132228 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.180104511718707 32.0 25.0 33.0 18.0 33.0 2 29.92734302388646 32.0 29.0 33.0 18.0 33.0 3 31.24615116036395 33.0 31.0 33.0 27.0 33.0 4 31.151400633090642 33.0 31.0 33.0 28.0 34.0 5 31.79962015220947 33.0 32.0 33.0 30.0 34.0 6 34.966024871936135 37.0 34.0 38.0 29.0 38.0 7 35.2854315794263 38.0 35.0 38.0 29.0 38.0 8 35.86268284770118 38.0 36.0 38.0 31.0 38.0 9 36.26682024109669 38.0 37.0 38.0 33.0 38.0 10-11 36.40626247709819 38.0 37.5 38.0 33.0 38.0 12-13 36.49131194204395 38.0 38.0 38.0 34.0 38.0 14-15 36.48417611340638 38.0 38.0 38.0 34.0 38.0 16-17 36.51408471716819 38.0 38.0 38.0 34.0 38.0 18-19 36.46980855453755 38.0 38.0 38.0 34.0 38.0 20-21 36.50615258064504 38.0 38.0 38.0 34.0 38.0 22-23 36.54698103216195 38.0 38.0 38.0 34.0 38.0 24-25 36.55244863071948 38.0 38.0 38.0 34.0 38.0 26-27 36.51020967826286 38.0 38.0 38.0 34.0 38.0 28-29 36.49483084867042 38.0 38.0 38.0 34.0 38.0 30-31 36.482636816344396 38.0 38.0 38.0 34.0 38.0 32-33 36.45370098959424 38.0 38.0 38.0 34.0 38.0 34-35 36.42768594812403 38.0 38.0 38.0 34.0 38.0 36-37 36.39420552402426 38.0 38.0 38.0 34.0 38.0 38-39 36.378157088386146 38.0 38.0 38.0 33.0 38.0 40-41 36.37629164273849 38.0 38.0 38.0 33.5 38.0 42-43 36.33641635745675 38.0 38.0 38.0 33.0 38.0 44-45 36.271034783552054 38.0 37.5 38.0 33.0 38.0 46-47 36.2101506584865 38.0 37.0 38.0 33.0 38.0 48-49 36.15520982110973 38.0 37.0 38.0 33.0 38.0 50-51 36.13037968181547 38.0 37.0 38.0 33.0 38.0 52-53 36.09767079356891 38.0 37.0 38.0 33.0 38.0 54-55 36.01393756907127 38.0 37.0 38.0 31.0 38.0 56-57 36.046145543028786 38.0 37.0 38.0 32.0 38.0 58-59 36.034264798482106 38.0 37.0 38.0 31.5 38.0 60-61 36.032681381783135 38.0 37.0 38.0 31.5 38.0 62-63 36.02043361039165 38.0 37.0 38.0 31.0 38.0 64-65 36.028090703576645 38.0 37.0 38.0 32.0 38.0 66-67 36.02361082713623 38.0 37.0 38.0 31.0 38.0 68-69 36.00809298640522 38.0 37.0 38.0 31.0 38.0 70-71 35.98713965102161 38.0 37.0 38.0 31.0 38.0 72-73 35.9773160176344 38.0 37.0 38.0 31.0 38.0 74-75 35.96163029625816 38.0 37.0 38.0 31.0 38.0 76-77 35.96211221855251 38.0 37.0 38.0 31.0 38.0 78-79 35.92521151595517 38.0 37.0 38.0 31.0 38.0 80-81 35.90428423715383 38.0 37.0 38.0 31.0 38.0 82-83 35.80724124927356 38.0 37.0 38.0 30.5 38.0 84-85 35.83055130828312 38.0 37.0 38.0 31.0 38.0 86-87 35.84575773490735 38.0 37.0 38.0 31.0 38.0 88-89 35.85677186363226 38.0 37.0 38.0 31.0 38.0 90-91 35.84471371971776 38.0 37.0 38.0 31.0 38.0 92-93 35.81889620073231 38.0 37.0 38.0 31.0 38.0 94-95 35.8216554764668 38.0 37.0 38.0 31.0 38.0 96-97 35.78652929873266 38.0 37.0 38.0 31.0 38.0 98-99 35.787255669233474 38.0 36.5 38.0 31.0 38.0 100-101 35.28384314973506 38.0 35.5 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 4.0 19 15.0 20 94.0 21 727.0 22 3530.0 23 10684.0 24 24563.0 25 45748.0 26 75080.0 27 112448.0 28 157697.0 29 210190.0 30 266837.0 31 335807.0 32 417934.0 33 533736.0 34 718998.0 35 1077924.0 36 2156327.0 37 5983884.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.57372644167254 19.699407231713746 14.316488282284176 25.410378044329534 2 13.771155636046405 20.531265980164566 43.33801672701832 22.35956165677071 3 17.241224116847743 28.263625374381217 30.414835202199857 24.080315306571183 4 12.18088713796015 19.577212033931442 38.35042500025552 29.891475827852886 5 13.561576065026317 38.26293277022009 34.683806698064416 13.491684466689168 6 28.285818565229732 38.82809488908385 19.200199666541053 13.685886879145365 7 25.809686398903814 34.02691574869843 22.92509669287455 17.238301159523214 8 24.60385676892983 36.31463239892953 21.065479481592334 18.01603135054831 9 26.092824829866366 17.00746144896057 21.351403880639236 35.548309840533825 10-11 24.051950721664646 27.28141113075026 28.361546617818263 20.305091529766834 12-13 25.09773967320759 24.92467170910405 28.891766623574828 21.08582199411353 14-15 22.425270939517457 25.858098776251154 26.893234284749678 24.82339599948171 16-17 21.207474302803153 29.34692805518468 27.38785357999741 22.057744062014763 18-19 21.274122115080594 27.970439559823635 29.846611850683978 20.90882647441179 20-21 22.5891930725991 26.770702034520948 29.694318230899512 20.945786661980435 22-23 22.31632573406111 26.76986651396078 29.573384926250355 21.340422825727757 24-25 21.740186725277276 27.145485406002706 29.699151549266144 21.415176319453874 26-27 21.600230568726573 27.23696147620712 29.75402676935566 21.408781185710644 28-29 21.551393951426665 27.106537314069318 29.83428841341119 21.507780321092827 30-31 21.756453249834127 27.026747642667694 29.715201296743317 21.501597810754873 32-33 21.515658777403875 27.327541524881017 29.627248421698006 21.529551276017106 34-35 21.755118990295816 27.198217821798597 29.616216587786464 21.430446600119122 36-37 21.674171442574995 27.11202700928803 29.773816559769116 21.439984988367858 38-39 21.557074573172606 27.18283168972311 29.77283638352156 21.487257353582727 40-41 21.70647417408878 27.02495144489074 29.75232003712966 21.51625434389082 42-43 21.82923892944919 27.022224727573345 29.48376883200277 21.66476751097469 44-45 21.610172956513253 27.297692260646212 29.30659554398003 21.785539238860505 46-47 21.88096804827208 27.13186185309946 28.915344866036314 22.07182523259215 48-49 21.76734247881353 27.354810899696965 28.876495584314032 22.001351037175475 50-51 21.596455469275554 27.545797215784084 28.80968976999803 22.048057544942328 52-53 21.775688779905774 27.437438809778815 28.52332550876045 22.26354690155496 54-55 21.81774716310273 27.206752926891774 28.55805368007046 22.417446229935038 56-57 21.86119650349222 27.088316678016973 28.7397362917916 22.31075052669921 58-59 21.571658349173603 27.025868376140995 29.093447737076783 22.309025537608616 60-61 21.715281247877847 26.902118071661796 29.15859557335786 22.224005107102496 62-63 21.385823260810454 27.48034411103214 29.0342667779663 22.09956585019111 64-65 21.011446637222626 27.695912697747772 28.878944482791486 22.41369618223812 66-67 21.185439696212974 28.227299339179883 28.346256693796118 22.241004270811022 68-69 21.290592066483757 28.318165387876416 28.043338484728388 22.347904060911443 70-71 21.28420963029307 27.843016133905063 28.121179532632834 22.751594703169033 72-73 21.280250183744915 27.462286494571273 28.067883957026616 23.189579364657188 74-75 21.515812553570832 27.275373009730092 28.12683957690058 23.0819748597985 76-77 21.96684735070056 27.053658695745007 27.972647408950653 23.006846544603782 78-79 22.152557088117362 26.992032447425075 27.297058925818828 23.558351538638732 80-81 22.14859134450663 27.328409254073442 27.14966454881794 23.37333485260199 82-83 22.09202922639333 27.386379801603923 27.327149458428906 23.19444151357385 84-85 22.14051536922624 27.460612061833384 27.176635518617182 23.222237050323194 86-87 22.269156933867055 27.90434992223034 27.07247656807043 22.754016575832168 88-89 22.567249688393442 27.532581532426192 27.36777320384966 22.532395575330703 90-91 22.35663879838868 27.4668873112721 27.68630584357724 22.490168046761976 92-93 22.15423520057012 27.152412983673642 27.470116091005515 23.223235724750722 94-95 21.94012219049635 27.146809491149316 28.07805284179612 22.835015476558215 96-97 21.626837014327695 27.364131686204367 28.258929905709905 22.75010139375803 98-99 21.8702646858592 27.539299439031577 28.52874647930912 22.0616893958001 100-101 22.709364238242284 30.196186446062544 26.02293088330601 21.07151843238916 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3119.0 1 2968.0 2 4348.0 3 6033.0 4 7278.0 5 10800.5 6 14322.5 7 18607.5 8 23730.0 9 25537.0 10 23300.5 11 21034.0 12 22515.0 13 28052.5 14 40381.5 15 56169.0 16 68770.5 17 75812.0 18 74674.0 19 66150.0 20 55270.5 21 45684.0 22 40750.5 23 42936.5 24 51236.5 25 65430.5 26 86492.0 27 109930.0 28 133948.5 29 161319.0 30 188470.5 31 216315.5 32 249236.0 33 284743.5 34 316824.5 35 344995.0 36 378498.5 37 410826.0 38 428767.5 39 436604.0 40 449785.0 41 462710.0 42 467612.0 43 476261.0 44 486962.0 45 500587.5 46 503672.0 47 494520.0 48 486987.5 49 475946.5 50 464133.5 51 453227.0 52 436456.5 53 416098.5 54 394448.5 55 368149.0 56 337970.5 57 301015.5 58 263551.0 59 228889.5 60 191696.0 61 153062.0 62 116720.0 63 87476.5 64 64334.5 65 46089.5 66 32425.0 67 22654.5 68 16131.5 69 11083.5 70 7258.5 71 4542.5 72 2716.0 73 1578.5 74 950.0 75 592.5 76 411.0 77 270.0 78 181.5 79 123.0 80 81.0 81 54.5 82 36.5 83 28.0 84 19.5 85 12.0 86 9.5 87 9.5 88 10.5 89 7.5 90 3.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.004475682455028046 4 0.0 5 0.051985505053152636 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.6072892794299613E-4 18-19 0.0 20-21 4.121333203483862E-6 22-23 0.0 24-25 0.0 26-27 2.968262594276349E-4 28-29 2.3095580689504538E-4 30-31 8.256565848218064E-6 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 9.296459181529592E-5 44-45 0.0 46-47 9.628738207159896E-4 48-49 0.0016051497368270243 50-51 0.002379974058282765 52-53 0.005567188706132533 54-55 0.007016700319372185 56-57 0.005562801449731296 58-59 0.007767889635094452 60-61 0.006803698221390305 62-63 0.0060351363675219495 64-65 0.005976916670386288 66-67 0.0012645199757084803 68-69 0.0023772274667344493 70-71 4.4188903375613585E-4 72-73 0.0016743352947669638 74-75 0.002237188958299273 76-77 5.277986764728462E-4 78-79 0.0 80-81 1.7175165241037986E-4 82-83 0.0 84-85 0.0 86-87 1.0154011992294323E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.464328898216043E-4 96-97 3.2545799126568386E-5 98-99 5.335734526201377E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 138.0 20-21 487.0 22-23 1075.0 24-25 1592.0 26-27 3636.0 28-29 8611.0 30-31 21244.0 32-33 27358.0 34-35 37118.0 36-37 57832.0 38-39 44694.0 40-41 75650.0 42-43 87469.0 44-45 105700.0 46-47 108602.0 48-49 99688.0 50-51 87533.0 52-53 10099.0 54-55 8475.0 56-57 7995.0 58-59 8579.0 60-61 102572.0 62-63 99540.0 64-65 106626.0 66-67 117061.0 68-69 118722.0 70-71 126446.0 72-73 105286.0 74-75 104494.0 76-77 110870.0 78-79 117811.0 80-81 120536.0 82-83 112786.0 84-85 109553.0 86-87 113334.0 88-89 120785.0 90-91 126989.0 92-93 124441.0 94-95 132671.0 96-97 202133.0 98-99 1120343.0 100-101 7935654.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.07214092771261 #Duplication Level Percentage of deduplicated Percentage of total 1 86.54201034791211 68.43062038397669 2 8.65211737841639 13.682828893385043 3 2.4300869578666515 5.764565351970848 4 1.000853521928496 3.165585229357102 5 0.4990350686862712 1.9729885639515787 6 0.27977616066915806 1.3273500002787617 7 0.16919562747203915 0.9365062349875288 8 0.1058957529186573 0.6698723118744244 9 0.06819179665540027 0.48528642197247585 >10 0.24564011764503066 2.9552627356447663 >50 0.005296367878649518 0.28003570451585064 >100 0.0017929730213776531 0.228993543724707 >500 6.475831611700393E-5 0.03403453493924241 >1k 4.317061356996146E-5 0.06607008942090609 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.4727527375845558E-5 0.0 8.242509125281852E-6 0.0 3 0.0 2.4727527375845558E-5 0.0 1.6485018250563704E-5 0.0 4 0.0 6.594007300225482E-5 0.0 1.6485018250563704E-5 0.0 5 0.0 6.594007300225482E-5 0.0 1.6485018250563704E-5 0.0 6 8.242509125281853E-5 7.418258212753667E-5 0.0 1.6485018250563704E-5 2.4727527375845558E-5 7 8.242509125281853E-5 8.242509125281853E-5 0.0 2.4727527375845558E-5 2.4727527375845558E-5 8 8.242509125281853E-5 9.066760037810038E-5 0.0 3.297003650112741E-5 3.297003650112741E-5 9 8.242509125281853E-5 1.895777098814826E-4 0.0 3.297003650112741E-5 3.297003650112741E-5 10-11 8.242509125281853E-5 2.0606272813204632E-4 0.0 6.181881843961389E-5 3.297003650112741E-5 12-13 8.242509125281853E-5 2.0606272813204632E-4 0.0 7.418258212753667E-5 3.297003650112741E-5 14-15 8.242509125281853E-5 3.090940921980695E-4 0.0 7.83038366901776E-5 6.18188184396139E-5 16-17 1.1539512775394593E-4 3.255791104486332E-4 0.0 8.242509125281853E-5 9.066760037810038E-5 18-19 1.1539512775394593E-4 3.5442789238711964E-4 0.0 8.242509125281853E-5 9.891010950338223E-5 20-21 1.1539512775394593E-4 3.791554197629652E-4 0.0 9.066760037810038E-5 1.0715261862866409E-4 22-23 1.1539512775394593E-4 4.203679653893745E-4 0.0 9.891010950338223E-5 1.4836516425507335E-4 24-25 1.1539512775394593E-4 4.574592564531428E-4 0.0 1.4424390969243242E-4 1.8133520075620075E-4 26-27 1.1539512775394593E-4 4.7806552926634745E-4 0.0 2.967303285101467E-4 1.8133520075620075E-4 28-29 1.1539512775394593E-4 4.7806552926634745E-4 0.0 0.001005586113284386 1.8133520075620075E-4 30-31 1.1539512775394593E-4 5.357630931433204E-4 0.0 0.003531915160183274 1.8545645531884168E-4 32-33 1.1951638231658685E-4 5.357630931433204E-4 0.0 0.008835969782302146 1.895777098814826E-4 34-35 1.2363763687922778E-4 6.14066929833498E-4 0.0 0.015586584755907983 2.184264918199691E-4 36-37 1.2363763687922778E-4 6.964920210863164E-4 0.0 0.025432261906057158 2.2666900094525094E-4 38-39 1.2363763687922778E-4 7.624320940885713E-4 0.0 0.05801077922373368 2.349115100705328E-4 40-41 1.2363763687922778E-4 8.654634581545946E-4 0.0 0.34188279349844064 2.3903276463317372E-4 42-43 1.401226551297915E-4 9.066760037810038E-4 0.0 0.3740120940687893 2.4315401919581463E-4 44-45 1.4836516425507335E-4 0.0010426774043481545 0.0 0.42796755880288434 2.4727527375845555E-4 46-47 1.4836516425507335E-4 0.0011415875138515367 0.0 0.48812551165375395 2.678815465716602E-4 48-49 1.4836516425507335E-4 0.0011498300229768185 0.0 0.548473042214505 3.0497283763542855E-4 50-51 1.4836516425507335E-4 0.0013311652237330191 0.0 0.6106792585830072 3.0497283763542855E-4 52-53 1.4836516425507335E-4 0.0013517714965462237 0.0 0.8148668158890519 3.420641286991969E-4 54-55 1.607289279429961E-4 0.0013600140056715055 0.0 1.4769339976136289 3.791554197629652E-4 56-57 1.730926916309189E-4 0.0014094690604231968 0.0 2.206709270547833 3.791554197629652E-4 58-59 1.8133520075620075E-4 0.0014135903149858377 0.0 2.9735098944728042 3.791554197629652E-4 60-61 1.895777098814826E-4 0.0014259540786737605 0.0 3.5617118306711677 3.791554197629652E-4 62-63 1.895777098814826E-4 0.0014671666243001697 0.0 3.5736717114119516 3.873979288882471E-4 64-65 1.895777098814826E-4 0.0016155317885552431 0.0 3.581366093680402 3.873979288882471E-4 66-67 2.1430523725732818E-4 0.001627895552243166 0.0 3.587865312125687 3.9564043801352893E-4 68-69 2.1430523725732818E-4 0.001677350606994857 0.0 3.5939936176603338 4.038829471388108E-4 70-71 2.1430523725732818E-4 0.0016979568798080617 0.0 3.5998746479212227 4.409742382025791E-4 72-73 2.184264918199691E-4 0.0016979568798080617 0.0 3.6041937227028704 5.357630931433204E-4 74-75 2.3079025550789187E-4 0.0017803819710608803 0.0 3.607816305463432 5.357630931433204E-4 76-77 2.3903276463317372E-4 0.0018710495714389804 0.0 3.610944337676476 5.357630931433204E-4 78-79 2.3903276463317372E-4 0.0019163833716280307 0.0 3.614224856308338 5.357630931433204E-4 80-81 2.4727527375845555E-4 0.0019246258807533126 0.0 3.615547779022946 5.522481113938841E-4 82-83 2.4727527375845555E-4 0.0019328683898785944 0.0 3.6161412396799664 5.687331296444478E-4 84-85 3.0497283763542855E-4 0.0019411108990038763 0.0 3.616878944246679 5.728543842070887E-4 86-87 3.0497283763542855E-4 0.002011172226568772 0.0 3.6170479156837474 5.852181478950115E-4 88-89 3.090940921980695E-4 0.0021595373908238455 0.0 3.6171262195204377 5.852181478950115E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22250 0.0 59.00035 1 GTATCAA 44830 0.0 41.78992 1 ATCAACG 56605 0.0 33.058342 3 TCAACGC 57080 0.0 32.797127 4 TATCAAC 57880 0.0 32.495453 2 CAACGCA 57885 0.0 32.318474 5 AACGCAG 59055 0.0 31.774908 6 GTGGTAT 8750 0.0 28.932125 1 TGGTATC 8605 0.0 28.86361 2 ACGCAGA 68020 0.0 27.54864 7 CGCAGAG 69420 0.0 26.98054 8 TATCTCG 17980 0.0 26.65157 94-95 GCAGAGT 77730 0.0 24.017782 9 GTACATG 63700 0.0 22.616026 1 TACATGG 63775 0.0 22.45984 2 GAGTACT 38755 0.0 22.324753 12-13 ACATGGG 63480 0.0 21.947517 3 CAGAGTA 75855 0.0 20.863083 10-11 CTATCTC 24655 0.0 20.40103 94-95 CATGGGG 42765 0.0 20.006144 4 >>END_MODULE