FastQCFastQC Report
Fri 27 May 2016
SRR2140382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8837722
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123900.053.8173451
GTATCAA239650.040.958211
TCAACGC339900.029.7925684
ATCAACG341850.029.6750933
TATCAAC340200.029.3293382
CAACGCA349050.029.0367015
AACGCAG363450.027.884596
GTGGTAT57250.026.9545141
TGGTATC57850.025.992922
TATCTCG130650.023.79105694-95
ACGCAGA437350.023.1728697
CGCAGAG447250.022.6501338
GCAGAGT507200.019.9902139
AGAGTAC462750.019.28769710-11
TACATGG420000.018.6317332
GTACATG419650.018.6159321
ACATGGG424700.018.239743
AGTACTT269650.018.14237212-13
CTATCTC182300.017.46766594-95
GAGTACT249100.017.21166412-13
Produced by FastQC (version 0.11.5)