##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8837722
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.073680525366154	28.0	18.0	32.0	18.0	33.0
2	27.63963847244799	29.0	25.0	32.0	18.0	33.0
3	29.593472277132047	31.0	28.0	33.0	25.0	33.0
4	29.135711555534332	31.0	29.0	33.0	15.0	33.0
5	31.024875075273922	33.0	32.0	33.0	27.0	33.0
6	33.89573433063407	37.0	33.0	38.0	26.0	38.0
7	35.47748820340807	38.0	36.0	38.0	30.0	38.0
8	35.94651811858304	38.0	36.0	38.0	31.0	38.0
9	36.20975857805892	38.0	37.0	38.0	33.0	38.0
10-11	36.34713651323271	38.0	37.0	38.0	33.0	38.0
12-13	36.40377735348544	38.0	37.0	38.0	33.0	38.0
14-15	36.42174425717396	38.0	37.0	38.0	33.5	38.0
16-17	36.462243041815526	38.0	38.0	38.0	34.0	38.0
18-19	36.418004718863074	38.0	38.0	38.0	33.5	38.0
20-21	36.45399262969716	38.0	38.0	38.0	33.5	38.0
22-23	36.50155982205317	38.0	38.0	38.0	34.0	38.0
24-25	36.50971053868322	38.0	38.0	38.0	34.0	38.0
26-27	36.46207885664191	38.0	38.0	38.0	34.0	38.0
28-29	36.44136757049156	38.0	38.0	38.0	34.0	38.0
30-31	36.428328524072285	38.0	38.0	38.0	34.0	38.0
32-33	36.39829440036638	38.0	38.0	38.0	34.0	38.0
34-35	36.3693255101099	38.0	38.0	38.0	33.0	38.0
36-37	36.33908382794519	38.0	37.5	38.0	33.0	38.0
38-39	36.32511290986279	38.0	38.0	38.0	33.0	38.0
40-41	36.323664037843926	38.0	38.0	38.0	33.0	38.0
42-43	36.287027771216984	38.0	37.0	38.0	33.0	38.0
44-45	36.22387282163654	38.0	37.0	38.0	33.0	38.0
46-47	36.16969086825835	38.0	37.0	38.0	33.0	38.0
48-49	36.11936919620414	38.0	37.0	38.0	33.0	38.0
50-51	36.09345913216887	38.0	37.0	38.0	33.0	38.0
52-53	36.06015676612923	38.0	37.0	38.0	32.5	38.0
54-55	35.971279541721145	38.0	37.0	38.0	31.0	38.0
56-57	36.00654460797233	38.0	37.0	38.0	31.0	38.0
58-59	35.987540895899	38.0	37.0	38.0	31.0	38.0
60-61	35.986371666347814	38.0	37.0	38.0	31.0	38.0
62-63	35.973169303120606	38.0	37.0	38.0	31.0	38.0
64-65	35.97962003179225	38.0	37.0	38.0	31.0	38.0
66-67	35.973426392673204	38.0	37.0	38.0	31.0	38.0
68-69	35.950551593014765	38.0	37.0	38.0	31.0	38.0
70-71	35.92792596707076	38.0	37.0	38.0	31.0	38.0
72-73	35.91553289655555	38.0	37.0	38.0	31.0	38.0
74-75	35.900943722835315	38.0	37.0	38.0	31.0	38.0
76-77	35.900872064545126	38.0	37.0	38.0	31.0	38.0
78-79	35.861427498394924	38.0	37.0	38.0	31.0	38.0
80-81	35.837377594508325	38.0	37.0	38.0	31.0	38.0
82-83	35.73656155039894	38.0	37.0	38.0	30.0	38.0
84-85	35.76119116854079	38.0	37.0	38.0	30.5	38.0
86-87	35.77650939387431	38.0	37.0	38.0	31.0	38.0
88-89	35.785954514484004	38.0	37.0	38.0	31.0	38.0
90-91	35.77178431488825	38.0	37.0	38.0	31.0	38.0
92-93	35.74353046298235	38.0	37.0	38.0	31.0	38.0
94-95	35.74869850468276	38.0	37.0	38.0	31.0	38.0
96-97	35.71876756945356	38.0	36.0	38.0	31.0	38.0
98-99	35.72291869054452	38.0	36.0	38.0	31.0	38.0
100-101	35.20721583338385	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	38.0
19	122.0
20	259.0
21	757.0
22	2969.0
23	8475.0
24	19209.0
25	35748.0
26	58614.0
27	86989.0
28	122251.0
29	162127.0
30	205231.0
31	256432.0
32	322442.0
33	414218.0
34	564493.0
35	872472.0
36	1836722.0
37	3868152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.60227782679745	23.716484858881056	13.859974323700158	23.82126299062134
2	13.723785382703824	24.38188257109694	38.22563099405028	23.668701052148958
3	19.282380244890753	27.138799716206446	29.781666800567137	23.797153238335667
4	13.994126540753374	18.530329421993585	37.72239045310545	29.75315358414759
5	13.888009002389845	39.673640726304484	32.9892643784083	13.449085892897372
6	29.74477243624233	38.36073802284548	18.25984323334036	13.634646307571826
7	26.343824800101203	33.51613685064997	22.392455883993637	17.74758246525519
8	25.160488189151003	36.28561749283356	20.68355397465546	17.870340343359974
9	26.01689666183209	16.760314479228924	20.824653683381307	36.398135175557684
10-11	24.5571822693676	27.14921333800724	28.029417535423722	20.264186857201437
12-13	25.412749202382084	24.52443766190188	28.497854339492367	21.564958796223664
14-15	22.62755606026078	25.63766997875697	26.434006410249154	25.300767550733095
16-17	21.794031790027923	28.75260594983417	27.02426765942778	22.42909460071013
18-19	21.915930372102675	27.76045682360228	29.09019428309693	21.23341852119811
20-21	23.036818767594706	26.731917545748917	28.82543212578264	21.405831560873732
22-23	22.59066963506732	26.699848434132445	28.85532803701493	21.854153893785302
24-25	22.240624150347443	26.96592166489887	28.90525565895127	21.88819852580242
26-27	22.13245465919666	27.07587953246743	28.89451450812894	21.897151300206975
28-29	22.085263098241274	26.98151167885315	29.04888583021375	21.884339392691825
30-31	22.331835642768784	26.838286252129944	28.86529681529673	21.964581289804542
32-33	22.08227849538293	27.154531583834924	28.79591808932614	21.967271831456006
34-35	22.257322599668377	27.085571710110944	28.845161994288038	21.81194369593264
36-37	22.21696327851227	26.852925743124995	28.985674762909447	21.94443621545329
38-39	22.07470652972965	27.03887514846683	28.999862038067743	21.886556283735782
40-41	22.28155767892219	26.864145289669622	28.93258575723394	21.92171127417425
42-43	22.346363182701527	26.85460369408365	28.719140756274903	22.07989236693992
44-45	22.15037835695345	27.096202969582723	28.5181982620064	22.235220411457426
46-47	22.377708813752914	26.868890968158944	28.23920402531629	22.514196192771855
48-49	22.198111863724982	27.087922565795818	28.298990891987035	22.414974678492165
50-51	22.097652435003813	27.209162011226574	28.231861701416033	22.461323852353583
52-53	22.262226274873704	27.185260994465267	27.963573458538484	22.58893927212255
54-55	22.288923045265907	26.916326197994593	28.063779341684715	22.73097141505479
56-57	22.312004659236223	26.84696652030884	28.240497891226557	22.60053092922838
58-59	22.052173243280972	26.885419123770642	28.44864271581998	22.6137649171284
60-61	22.221346725615877	26.71582989033902	28.525278255077612	22.537545128967487
62-63	21.91889331246084	27.29143734330648	28.45974461309503	22.329924731137655
64-65	21.49544360477439	27.503744467650655	28.33195967170765	22.668852255867304
66-67	21.67649967302419	27.949501042279895	27.7966956467797	22.57730363791622
68-69	21.758572925276535	28.026459766365335	27.519686782650115	22.69528052570802
70-71	21.732618729389586	27.552701471939496	27.662040022068723	23.052639776602195
72-73	21.777351246620118	27.172853305682466	27.54124415366413	23.508551294033285
74-75	21.924869269977517	27.048300259961565	27.68215275376545	23.34467771629547
76-77	22.358037301832915	26.886591115955056	27.516947320491703	23.238424261720326
78-79	22.550847559539914	26.82299738910669	26.83637024124428	23.78978481010911
80-81	22.558395300225264	27.142750768714496	26.736930093295246	23.56192383776499
82-83	22.50815230309781	27.18565790919109	26.880354460339156	23.425835327371942
84-85	22.577269812945193	27.196095044093365	26.777511145537446	23.449123997423996
86-87	22.655852528497135	27.662217213645718	26.682850857981492	22.999079399875654
88-89	22.894971398523698	27.36010168551418	26.984144693685696	22.76078222227643
90-91	22.707628723528767	27.29694001266904	27.314962502351865	22.680468761450328
92-93	22.579223770844596	26.909520683459494	27.062364131071476	23.448891414624434
94-95	22.332766156399178	26.976133914637558	27.642724408025405	23.04837552093786
96-97	21.993571468651112	27.199958029429162	27.826276860855227	22.9801936410645
98-99	22.2760404175226	27.420719352833622	28.070504732409447	22.232735497234334
100-101	23.154182287866206	30.144332095619113	25.410610108724065	21.290875507790616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1897.0
1	1844.0
2	2473.0
3	3549.5
4	4430.0
5	5945.0
6	7960.0
7	11927.0
8	15122.5
9	15528.0
10	15001.0
11	14197.5
12	15133.0
13	19192.5
14	28872.0
15	40481.5
16	46816.0
17	46842.0
18	42837.5
19	36854.5
20	30932.0
21	26338.5
22	24468.5
23	26729.0
24	32758.5
25	43062.0
26	58089.0
27	74738.5
28	90258.5
29	106863.0
30	122020.5
31	137811.5
32	159280.5
33	183060.5
34	204785.0
35	225868.5
36	251743.0
37	274927.5
38	287635.0
39	295138.0
40	307101.5
41	318604.5
42	325790.5
43	335340.0
44	346425.5
45	360305.0
46	366996.5
47	365376.0
48	366061.5
49	362863.0
50	354514.0
51	346982.0
52	336268.5
53	323450.0
54	310790.0
55	292957.0
56	272283.5
57	246342.5
58	219756.0
59	193329.5
60	161009.0
61	127852.0
62	96335.5
63	70416.5
64	51216.0
65	36424.5
66	25344.0
67	17518.5
68	12170.0
69	8262.0
70	5265.5
71	3169.0
72	1868.0
73	1086.0
74	687.5
75	446.5
76	291.5
77	191.5
78	127.5
79	83.5
80	54.5
81	40.0
82	32.5
83	23.5
84	13.5
85	9.5
86	8.5
87	8.5
88	8.0
89	6.5
90	4.5
91	2.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003530321501400474
4	0.0
5	0.05105388017409917
6	1.1315133017309213E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.6575665086546064E-6
14-15	0.0
16-17	1.5275429573367435E-4
18-19	0.0
20-21	1.1315376283786017E-5
22-23	0.0
24-25	0.0
26-27	3.0560254351865107E-4
28-29	2.151334005417625E-4
30-31	5.666630645784528E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.265922894896694E-5
44-45	0.0
46-47	9.878787280073497E-4
48-49	0.0016004817094282776
50-51	0.0024785662252887696
52-53	0.005602642237304986
54-55	0.007271189779375277
56-57	0.005946967719931374
58-59	0.007870136490309022
60-61	0.006980022836800732
62-63	0.005742830571836879
64-65	0.0061159220577568785
66-67	0.0014239016502462947
68-69	0.0018449373324820352
70-71	3.160611798361564E-4
72-73	0.0021074912500795096
74-75	0.002352392221766782
76-77	7.936801720022033E-4
78-79	0.0
80-81	2.1920047357930196E-4
82-83	0.0
84-85	6.783959517671332E-6
86-87	8.911236747362942E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	6.183947263872985E-4
96-97	5.107681602889722E-5
98-99	4.6848586275903114E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	113.0
20-21	381.0
22-23	677.0
24-25	1103.0
26-27	2439.0
28-29	6028.0
30-31	14226.0
32-33	18246.0
34-35	25407.0
36-37	39717.0
38-39	31191.0
40-41	50962.0
42-43	57573.0
44-45	68749.0
46-47	69383.0
48-49	64795.0
50-51	58696.0
52-53	6203.0
54-55	5357.0
56-57	5227.0
58-59	5681.0
60-61	69767.0
62-63	67497.0
64-65	73263.0
66-67	80515.0
68-69	80638.0
70-71	86717.0
72-73	71572.0
74-75	71340.0
76-77	75636.0
78-79	80529.0
80-81	82235.0
82-83	76792.0
84-85	75706.0
86-87	77768.0
88-89	83793.0
90-91	87910.0
92-93	87799.0
94-95	94710.0
96-97	148557.0
98-99	840278.0
100-101	5892546.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.23737537041984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42415145003291	71.02108607786757
2	8.481117986232443	13.779675308167686
3	2.1999839544770974	5.361627669562698
4	0.8564540364586315	2.7830431218920437
5	0.392547272256796	1.5944755053479864
6	0.22393219605644638	1.091499832113599
7	0.11341762223410286	0.6449624965736602
8	0.07986275665749	0.5190272592560808
9	0.05207268752874098	0.380722361698702
>10	0.1691956477793665	2.1246077552461986
>50	0.005042624428728317	0.2800926533374765
>100	0.002042161369604796	0.27135651863122073
>500	1.2828904148551648E-4	0.0636567379214685
>1k	5.1315446180011875E-5	0.08416670238361301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.789079810385526E-5	0.0	3.394539905192763E-5	0.0
3	0.0	6.789079810385526E-5	0.0	4.5260532069236845E-5	0.0
4	0.0	1.8104212827694738E-4	0.0	7.920593112116448E-5	0.0
5	0.0	1.8104212827694738E-4	0.0	9.052106413847369E-5	0.0
6	0.0	1.8104212827694738E-4	0.0	1.018361971557829E-4	0.0
7	0.0	1.8104212827694738E-4	0.0	1.018361971557829E-4	0.0
8	0.0	2.1498752732887502E-4	0.0	1.018361971557829E-4	1.1315133017309211E-5
9	0.0	4.186599216404408E-4	0.0	2.4893292638080263E-4	1.1315133017309211E-5
10-11	0.0	4.186599216404408E-4	0.0	3.111661579760033E-4	1.1315133017309211E-5
12-13	0.0	4.186599216404408E-4	0.0	3.6774182306254936E-4	1.1315133017309211E-5
14-15	0.0	5.940444834087336E-4	0.0	3.847145225885132E-4	2.8287832543273025E-5
16-17	0.0	6.110171829346974E-4	0.0	4.130023551317862E-4	3.394539905192763E-5
18-19	0.0	6.675928480212435E-4	0.0	4.639204537096777E-4	3.960296556058224E-5
20-21	0.0	7.354836461250987E-4	0.0	4.808931532356415E-4	4.5260532069236845E-5
22-23	0.0	8.599501093155001E-4	0.0	5.148385522875691E-4	4.5260532069236845E-5
24-25	0.0	9.278409074193553E-4	0.0	6.053596164260428E-4	4.5260532069236845E-5
26-27	0.0	9.731014394885922E-4	0.0	7.920593112116448E-4	4.5260532069236845E-5
28-29	0.0	9.731014394885922E-4	0.0	0.0015558307898800167	4.5260532069236845E-5
30-31	0.0	9.957317055232105E-4	0.0	0.0038697754919197504	4.5260532069236845E-5
32-33	1.1315133017309211E-5	0.0010127044050491744	0.0	0.008559898127594417	4.5260532069236845E-5
34-35	1.1315133017309211E-5	0.001148486001256885	0.0	0.014839796952201032	6.789079810385526E-5
36-37	1.1315133017309211E-5	0.0012672948979386316	0.0	0.024135178725920546	6.789079810385526E-5
38-39	1.1315133017309211E-5	0.0013012402969905593	0.0	0.05433526874911883	6.789079810385526E-5
40-41	1.1315133017309211E-5	0.0014709672922501975	0.0	0.3201673462912728	6.789079810385526E-5
42-43	1.1315133017309211E-5	0.0015275429573367435	0.0	0.3498582553286922	7.354836461250987E-5
44-45	1.1315133017309211E-5	0.0017425304846656186	0.0	0.4003350637189086	7.920593112116448E-5
46-47	1.6972699525963816E-5	0.001923572612942566	0.0	0.4562148481248901	7.920593112116448E-5
48-49	2.2630266034618422E-5	0.0019348877459598752	0.0	0.5137805873504507	9.052106413847369E-5
50-51	2.2630266034618422E-5	0.0022177660713926057	0.0	0.5731228024597289	9.052106413847369E-5
52-53	2.2630266034618422E-5	0.002251711470444533	0.0	0.7646087985116528	1.018361971557829E-4
54-55	2.2630266034618422E-5	0.0022573690369531874	0.0	1.3857247376642987	1.1315133017309212E-4
56-57	2.2630266034618422E-5	0.002308287135531079	0.0	2.0606837372798106	1.1315133017309212E-4
58-59	2.2630266034618422E-5	0.002325259835057043	0.0	2.7767449575806977	1.2446646319040131E-4
60-61	2.2630266034618422E-5	0.0023648628006176253	0.0	3.328963051790948	1.3578159620771053E-4
62-63	2.2630266034618422E-5	0.002427096032212826	0.0	3.340572378266707	1.3578159620771053E-4
64-65	2.2630266034618422E-5	0.0026816865251022832	0.0	3.347949844993993	1.3578159620771053E-4
66-67	2.2630266034618422E-5	0.002709974357645556	0.0	3.354597485641662	1.3578159620771053E-4
68-69	2.2630266034618422E-5	0.0027665500227321022	0.0	3.360362545913981	1.4143916271636513E-4
70-71	2.2630266034618422E-5	0.002811810554801339	0.0	3.3656184252005215	1.4709672922501974E-4
72-73	2.2630266034618422E-5	0.002828783254327303	0.0	3.3698219971164516	2.1498752732887502E-4
74-75	2.2630266034618422E-5	0.0028910164859225033	0.0	3.3733919215834125	2.1498752732887502E-4
76-77	2.2630266034618422E-5	0.0030607434811821418	0.0	3.3764752953306294	2.1498752732887502E-4
78-79	2.2630266034618422E-5	0.0031456069788119607	0.0	3.3795812993438807	2.1498752732887502E-4
80-81	2.2630266034618422E-5	0.0031456069788119607	0.0	3.381086212035183	2.2064509383752962E-4
82-83	2.2630266034618422E-5	0.0031512645453206155	0.0	3.381872613779886	2.2630266034618423E-4
84-85	2.2630266034618422E-5	0.0031795523778638886	0.0	3.382461000696786	2.2630266034618423E-4
86-87	2.2630266034618422E-5	0.0032927037080369807	0.0	3.382647700391572	2.3196022685483884E-4
88-89	2.2630266034618422E-5	0.0034511155702793097	0.0	3.3827608517217445	2.5459049288945724E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12390	0.0	53.817345	1
GTATCAA	23965	0.0	40.95821	1
TCAACGC	33990	0.0	29.792568	4
ATCAACG	34185	0.0	29.675093	3
TATCAAC	34020	0.0	29.329338	2
CAACGCA	34905	0.0	29.036701	5
AACGCAG	36345	0.0	27.88459	6
GTGGTAT	5725	0.0	26.954514	1
TGGTATC	5785	0.0	25.99292	2
TATCTCG	13065	0.0	23.791056	94-95
ACGCAGA	43735	0.0	23.172869	7
CGCAGAG	44725	0.0	22.650133	8
GCAGAGT	50720	0.0	19.990213	9
AGAGTAC	46275	0.0	19.287697	10-11
TACATGG	42000	0.0	18.631733	2
GTACATG	41965	0.0	18.615932	1
ACATGGG	42470	0.0	18.23974	3
AGTACTT	26965	0.0	18.142372	12-13
CTATCTC	18230	0.0	17.467665	94-95
GAGTACT	24910	0.0	17.211664	12-13
>>END_MODULE