FastQCFastQC Report
Fri 27 May 2016
SRR2140381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7580329
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101650.054.9559361
GTATCAA187100.035.7295151
ATCAACG235250.030.5173073
TCAACGC236650.030.4855334
TATCAAC237800.029.6148362
CAACGCA243850.029.549225
AACGCAG253350.028.4634446
ACGCAGA311350.023.1186187
CGCAGAG314350.022.8839558
GTGGTAT49150.020.1998081
TGGTATC50450.019.9416892
GAGTACT171850.019.8133712-13
GCAGAGT366500.019.6397849
TATCTCG128400.019.2008494-95
CAGAGTA354900.018.41759110-11
GTACTTT187850.017.79748314-15
AGAGTAC331200.016.60606210-11
TACATGG285800.015.9796192
ACATGGG291750.015.4419863
GTACATG291500.015.2433061
Produced by FastQC (version 0.11.5)