##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7580329
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.258132199802937	25.0	18.0	32.0	18.0	33.0
2	26.482602008435254	27.0	18.0	31.0	18.0	33.0
3	28.845727144560612	31.0	27.0	32.0	18.0	33.0
4	29.684554324752924	31.0	29.0	33.0	25.0	33.0
5	30.533766146561714	32.0	31.0	33.0	25.0	33.0
6	34.82901203892337	37.0	34.0	38.0	29.0	38.0
7	35.18125928307333	38.0	35.0	38.0	29.0	38.0
8	36.04468038260608	38.0	37.0	38.0	32.0	38.0
9	36.317118030101334	38.0	37.0	38.0	33.0	38.0
10-11	36.415437311494	38.0	37.5	38.0	33.5	38.0
12-13	36.4707023929964	38.0	38.0	38.0	34.0	38.0
14-15	36.49077757706822	38.0	38.0	38.0	34.0	38.0
16-17	36.51174408129251	38.0	38.0	38.0	34.0	38.0
18-19	36.46678752333837	38.0	38.0	38.0	34.0	38.0
20-21	36.5018181474687	38.0	38.0	38.0	34.0	38.0
22-23	36.5389072122676	38.0	38.0	38.0	34.0	38.0
24-25	36.54576138378665	38.0	38.0	38.0	34.0	38.0
26-27	36.49386029267416	38.0	38.0	38.0	34.0	38.0
28-29	36.47359290079608	38.0	38.0	38.0	34.0	38.0
30-31	36.45955847059364	38.0	38.0	38.0	34.0	38.0
32-33	36.42597724547009	38.0	38.0	38.0	34.0	38.0
34-35	36.40049432876046	38.0	38.0	38.0	34.0	38.0
36-37	36.36975142303465	38.0	38.0	38.0	33.0	38.0
38-39	36.3468849390228	38.0	38.0	38.0	33.0	38.0
40-41	36.35962837225132	38.0	38.0	38.0	33.0	38.0
42-43	36.324447449214915	38.0	37.5	38.0	33.0	38.0
44-45	36.26599477946847	38.0	37.0	38.0	33.0	38.0
46-47	36.221984047551175	38.0	37.0	38.0	33.0	38.0
48-49	36.17883134177714	38.0	37.0	38.0	33.0	38.0
50-51	36.152989033246214	38.0	37.0	38.0	33.0	38.0
52-53	36.12020955213453	38.0	37.0	38.0	33.0	38.0
54-55	36.029846310199616	38.0	37.0	38.0	31.0	38.0
56-57	36.0597487418359	38.0	37.0	38.0	32.5	38.0
58-59	36.04091540645037	38.0	37.0	38.0	31.5	38.0
60-61	36.03491729047719	38.0	37.0	38.0	31.5	38.0
62-63	36.01764616486025	38.0	37.0	38.0	31.0	38.0
64-65	36.02155158705435	38.0	37.0	38.0	31.0	38.0
66-67	36.00821032693676	38.0	37.0	38.0	31.0	38.0
68-69	35.98322542989595	38.0	37.0	38.0	31.0	38.0
70-71	35.960033159522546	38.0	37.0	38.0	31.0	38.0
72-73	35.94592872841163	38.0	37.0	38.0	31.0	38.0
74-75	35.9300661267605	38.0	37.0	38.0	31.0	38.0
76-77	35.92913916177071	38.0	37.0	38.0	31.0	38.0
78-79	35.88922516498138	38.0	37.0	38.0	31.0	38.0
80-81	35.86963623551608	38.0	37.0	38.0	31.0	38.0
82-83	35.76999193673749	38.0	37.0	38.0	30.0	38.0
84-85	35.79200091662923	38.0	37.0	38.0	31.0	38.0
86-87	35.804896568023935	38.0	37.0	38.0	31.0	38.0
88-89	35.812723556627375	38.0	37.0	38.0	31.0	38.0
90-91	35.80204154527847	38.0	37.0	38.0	31.0	38.0
92-93	35.77107472512902	38.0	37.0	38.0	31.0	38.0
94-95	35.77520716379834	38.0	37.0	38.0	31.0	38.0
96-97	35.75040622654764	38.0	37.0	38.0	31.0	38.0
98-99	35.75038016442703	38.0	36.0	38.0	31.0	38.0
100-101	35.21431692459679	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	64.0
19	157.0
20	285.0
21	776.0
22	2653.0
23	7413.0
24	16388.0
25	30171.0
26	49233.0
27	72240.0
28	101198.0
29	133728.0
30	169299.0
31	212707.0
32	269224.0
33	350320.0
34	484283.0
35	759477.0
36	1672794.0
37	3247912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.379994720545774	26.913831840280285	13.517988467255183	24.188184971918766
2	13.966227587219498	20.52837020662296	38.61389129680256	26.891510909354988
3	16.69091881015598	25.963425744773872	32.52316662370321	24.822488821366935
4	13.324025909693365	18.99776381737521	38.338019365650226	29.3401909072812
5	13.82990868860588	36.905942387622105	34.991706703309305	14.272442220462711
6	29.026171426882847	38.983379083332544	18.57638614054695	13.41406334923766
7	25.73067738880463	33.456687697855855	23.1045380747986	17.708096838540914
8	24.17926715318029	37.15950851209756	20.99399643471939	17.667227900002757
9	25.203906057375608	16.749523668431806	21.25256040997693	36.79400986421565
10-11	23.899239729568468	27.137608407234037	28.748475165128056	20.214676698069436
12-13	24.76556096707676	24.550227305437534	29.042347634251758	21.64186409323395
14-15	22.203013879740578	25.77556989940674	26.478956256384123	25.542459964468563
16-17	21.518630738905287	29.05136483648056	27.26371650175283	22.166287922861326
18-19	21.601549220357057	28.09726992060635	28.980655061277684	21.32052579775891
20-21	22.631354538059618	27.071500319123647	28.83240586512059	21.464739277696147
22-23	22.218328796341453	27.17579528079403	28.786209163032687	21.819666759831833
24-25	21.955591948147767	27.395061086602112	28.892149913788963	21.757197051461155
26-27	21.928707178424318	27.492612180447278	28.851437856937185	21.727242784191215
28-29	21.850709816464068	27.36231207979296	28.950524479366834	21.836453624376134
30-31	22.020988480120877	27.15570301355451	28.94726221808714	21.876046288237468
32-33	21.777710192846794	27.543568358240467	28.77364349966061	21.905077949252124
34-35	21.965345499280716	27.40907414903179	28.857630635624098	21.76794971606339
36-37	21.918706286444923	27.316580423305215	29.024767179474907	21.739946110774955
38-39	21.798389600667846	27.323321797682137	29.038539112958876	21.839749488691137
40-41	21.954161769727364	27.19954603975312	29.067352542745706	21.77893964777381
42-43	22.088283637784304	27.18585264384125	28.818286478081095	21.907577240293346
44-45	21.86329518294613	27.459004963458426	28.65790044435828	22.019799409237162
46-47	22.121597085178518	27.147983207820975	28.383886711753824	22.346532995246687
48-49	21.99430650800416	27.361765738978427	28.450422474514276	22.193505278503135
50-51	21.7928828724997	27.467215681527158	28.479282179100345	22.260619266872798
52-53	21.87635207676214	27.43320358950519	28.376415115822475	22.314029217910203
54-55	21.93295077645431	27.25397865753587	28.270571289338946	22.542499276670874
56-57	22.031945602454538	27.19629144544774	28.373317000873243	22.398445951224478
58-59	21.674142732788745	27.368619215639995	28.73812984272061	22.21910820885065
60-61	21.92614088376299	27.34990308772464	28.563662762570086	22.160293265942286
62-63	21.614133261328835	27.861490341347956	28.459959814972418	22.064416582350788
64-65	21.221356720383596	28.036107643031272	28.414299035541863	22.328236601043265
66-67	21.40287332971031	28.414589858627902	27.960862233209383	22.2216745784524
68-69	21.45866125160122	28.40067069362744	27.75536289068578	22.38530516408556
70-71	21.503696972489532	27.81472384837426	27.86336745579352	22.818211723342692
72-73	21.52102035837377	27.525215909928562	27.891419060959066	23.062344670738604
74-75	21.72701312754777	27.346243398093566	27.927127993033814	22.999615481324852
76-77	22.057994080506255	27.233641132826374	27.76542032315576	22.942944463511612
78-79	22.236767946183843	27.229179448041187	27.199074940340985	23.334977665433986
80-81	22.246567372052432	27.415682807004682	27.122853796239877	23.21489602470301
82-83	22.19308765737505	27.48281601869384	27.325162337399394	22.998933986531718
84-85	22.29436422066979	27.55361869803481	27.147782967748146	23.004234113547255
86-87	22.36376307453099	27.888757670589403	27.08637948022928	22.661099774650328
88-89	22.55409636961515	27.63750586294252	27.33218796752597	22.47620979991636
90-91	22.484081329507504	27.57056937868647	27.594201683163096	22.351147608642933
92-93	22.278549315936242	27.24652096764822	27.37043298913779	23.104496727277752
94-95	22.023976420560736	27.294528055181388	27.926902488467565	22.754593035790315
96-97	21.754703060063278	27.54947331563129	28.10777125182891	22.588052372476522
98-99	22.04619886791516	27.797963612740922	28.252683667248707	21.903153852095212
100-101	22.797435018385052	30.47997342922975	25.68018079946827	21.042410752916922
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1364.0
1	1444.0
2	2086.0
3	2736.5
4	3215.5
5	4071.5
6	5125.5
7	6616.0
8	8039.0
9	8928.0
10	8973.0
11	8898.0
12	10004.5
13	12788.5
14	18458.0
15	25231.5
16	29924.0
17	31526.0
18	30559.0
19	28006.0
20	24662.5
21	21899.0
22	21438.0
23	24346.0
24	30307.0
25	40041.5
26	54015.5
27	69712.0
28	85288.5
29	101626.5
30	115723.0
31	130361.5
32	150077.5
33	171908.5
34	190969.5
35	207907.0
36	226990.5
37	245970.5
38	258008.5
39	262815.0
40	270702.0
41	279060.5
42	285011.5
43	292926.5
44	302114.5
45	316461.0
46	325396.5
47	323472.0
48	319502.5
49	310731.5
50	299422.0
51	288220.5
52	273003.0
53	258533.0
54	245524.5
55	229812.5
56	212200.5
57	189833.5
58	166842.0
59	144201.0
60	118961.5
61	93451.0
62	69306.0
63	50671.0
64	36809.0
65	26061.0
66	18932.0
67	14085.0
68	10368.5
69	7128.0
70	4577.5
71	2834.5
72	1723.5
73	995.5
74	589.0
75	365.5
76	238.0
77	157.5
78	105.0
79	71.5
80	47.5
81	33.0
82	26.5
83	17.5
84	9.5
85	5.5
86	6.5
87	7.5
88	4.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0032056656116113166
4	0.0
5	0.0491799234571481
6	1.3192039553956034E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.234427687769225E-5
18-19	0.0
20-21	1.978856446563521E-5
22-23	0.0
24-25	0.0
26-27	2.3752868818711773E-4
28-29	2.2440252004030006E-4
30-31	6.605093980579703E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.1439230099440553E-4
44-45	0.0
46-47	0.0010224564844224324
48-49	0.0015650645736719484
50-51	0.002321595732243731
52-53	0.005637095979195506
54-55	0.0068976353405660554
56-57	0.005789329161309684
58-59	0.007384997953827073
60-61	0.006866065097128333
62-63	0.005401450328045039
64-65	0.005776390814745767
66-67	0.0012928987926682494
68-69	0.0018611596539541528
70-71	4.3014995391927485E-4
72-73	0.0019587791857178195
74-75	0.0022426040107858857
76-77	6.366778419856978E-4
78-79	0.0
80-81	1.7562074105386037E-4
82-83	0.0
84-85	0.0
86-87	8.649444312518576E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	7.15350576461562E-4
96-97	4.1684538912725497E-5
98-99	5.689355990762555E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	127.0
20-21	346.0
22-23	611.0
24-25	889.0
26-27	1803.0
28-29	4167.0
30-31	10263.0
32-33	13115.0
34-35	18291.0
36-37	29427.0
38-39	22968.0
40-41	37981.0
42-43	41877.0
44-45	49937.0
46-47	51859.0
48-49	49045.0
50-51	44246.0
52-53	4309.0
54-55	3831.0
56-57	3931.0
58-59	4436.0
60-61	54561.0
62-63	54775.0
64-65	62546.0
66-67	67815.0
68-69	66368.0
70-71	76569.0
72-73	56977.0
74-75	57069.0
76-77	60629.0
78-79	64645.0
80-81	66558.0
82-83	62396.0
84-85	61428.0
86-87	63947.0
88-89	68185.0
90-91	71959.0
92-93	71042.0
94-95	78020.0
96-97	124367.0
98-99	714121.0
100-101	5182893.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.37600167550335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.24030839305055	70.99267481964311
2	8.492787682542035	13.822182093684699
3	2.301105335043159	5.617642547999456
4	0.9070742652335102	2.9525630770979236
5	0.4097744632910673	1.667290370567619
6	0.23135688135705793	1.1296138778970706
7	0.12325388690920122	0.7020935955344821
8	0.08423994226236649	0.5484087746149312
9	0.052215962062902355	0.3824213594686885
>10	0.15485587185038283	1.8546363116750342
>50	0.0022441793629383137	0.12560247744798203
>100	6.703475942807078E-4	0.11219227032337198
>500	5.63947244759268E-5	0.0326519952838911
>1k	5.639471608758907E-5	0.060026428761698045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.638407910791207E-5
2	0.0	2.638407910791207E-5	0.0	0.0	2.638407910791207E-5
3	0.0	3.957611866186811E-5	0.0	0.0	2.638407910791207E-5
4	0.0	2.1107263286329657E-4	0.0	0.0	2.638407910791207E-5
5	0.0	2.242646724172526E-4	0.0	0.0	3.957611866186811E-5
6	0.0	2.242646724172526E-4	0.0	0.0	3.957611866186811E-5
7	0.0	2.242646724172526E-4	0.0	0.0	6.596019776978018E-5
8	0.0	2.242646724172526E-4	0.0	0.0	6.596019776978018E-5
9	0.0	3.5618506795681294E-4	0.0	0.0	6.596019776978018E-5
10-11	0.0	3.5618506795681294E-4	0.0	0.0	6.596019776978018E-5
12-13	0.0	3.5618506795681294E-4	0.0	0.0	6.596019776978018E-5
14-15	0.0	5.540656612661534E-4	0.0	0.0	1.3851641531653836E-4
16-17	0.0	5.936417799280216E-4	0.0	0.0	1.7149651420142847E-4
18-19	0.0	6.332178985898897E-4	0.0	6.596019776978018E-6	1.780925339784065E-4
20-21	0.0	6.793900370287359E-4	0.0	2.638407910791207E-5	2.0447661308631857E-4
22-23	0.0	7.71734313906428E-4	0.0	3.957611866186811E-5	2.4405273174818665E-4
24-25	0.0	8.179064523452743E-4	0.0	6.596019776978018E-5	2.638407910791207E-4
26-27	0.0	8.442905314531863E-4	0.0	1.7149651420142847E-4	2.638407910791207E-4
28-29	0.0	8.442905314531863E-4	0.0	8.706746105610983E-4	3.0341690974098885E-4
30-31	0.0	9.630188874387906E-4	0.0	0.002869268602985438	3.69377107510769E-4
32-33	0.0	9.894029665467026E-4	0.0	0.006767516291179446	4.089532261726371E-4
34-35	0.0	0.001160899480748131	0.0	0.01197837191499208	4.2874128550357115E-4
36-37	0.0	0.0013389920147265375	0.0	0.01982763544959592	4.3533730528054915E-4
38-39	0.0	0.0013719721136114277	0.0	0.04502443099765195	4.4193332505752716E-4
40-41	0.0	0.001503892509150988	0.0	0.274255906306969	5.144895426042854E-4
42-43	0.0	0.001536872608035878	0.0	0.29935376155837035	6.530059579208237E-4
44-45	0.0	0.0017479452408991746	0.0	0.341779360763893	6.925820765826919E-4
46-47	0.0	0.0019260377748775812	0.0	0.3896664643447534	7.123701359136259E-4
48-49	0.0	0.0019458258342085152	0.0	0.43725674703564976	7.25562175467582E-4
50-51	0.0	0.002269030803280438	0.0	0.4868522197387475	7.25562175467582E-4
52-53	0.0	0.0023086069219423063	0.0	0.655321160862543	7.38754215021538E-4
54-55	0.0	0.0023152029417192843	0.0	1.2140502081110198	7.51946254575494E-4
56-57	0.0	0.0023613750801581303	0.0	1.8290367080373424	7.51946254575494E-4
58-59	0.0	0.0023943551790430203	0.0	2.4780652660326483	7.58542274352472E-4
60-61	0.0	0.002466911396589779	0.0	2.9790065840150213	7.783303336834061E-4
62-63	0.0	0.002532871594359559	0.0	2.9893887191439843	8.047144127913182E-4
64-65	0.0	0.0026911760690070314	0.0	2.996149639415387	8.047144127913182E-4
66-67	0.0	0.0026977720887840094	0.0	3.002132229353106	8.113104325682962E-4
68-69	0.0	0.0027571362667768115	0.0	3.0072111645813786	8.245024721222523E-4
70-71	0.0	0.0027835203458847235	0.0	3.0126858609962706	8.376945116762083E-4
72-73	0.0	0.0027901163656617015	0.0	3.016524744506472	8.970586896690104E-4
74-75	0.0	0.002856076563431482	0.0	3.0197765822565223	8.970586896690104E-4
76-77	0.0	0.002987996958971042	0.0	3.0228833075714787	8.970586896690104E-4
78-79	0.0	0.0030605531765178	0.0	3.0256998080162485	8.970586896690104E-4
80-81	0.0	0.003067149196294778	0.0	3.026933263714543	9.168467489999444E-4
82-83	0.0	0.003080341235848734	0.0	3.0274807333560325	9.234427687769225E-4
84-85	0.0	0.0031528974533954926	0.0	3.027843514443766	9.234427687769225E-4
86-87	0.0	0.0031858775522803826	0.0	3.028127143294176	9.366348083308786E-4
88-89	0.0	0.0035222745609062613	0.0	3.0281667194128383	9.828069467697246E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10165	0.0	54.955936	1
GTATCAA	18710	0.0	35.729515	1
ATCAACG	23525	0.0	30.517307	3
TCAACGC	23665	0.0	30.485533	4
TATCAAC	23780	0.0	29.614836	2
CAACGCA	24385	0.0	29.54922	5
AACGCAG	25335	0.0	28.463444	6
ACGCAGA	31135	0.0	23.118618	7
CGCAGAG	31435	0.0	22.883955	8
GTGGTAT	4915	0.0	20.199808	1
TGGTATC	5045	0.0	19.941689	2
GAGTACT	17185	0.0	19.81337	12-13
GCAGAGT	36650	0.0	19.639784	9
TATCTCG	12840	0.0	19.20084	94-95
CAGAGTA	35490	0.0	18.417591	10-11
GTACTTT	18785	0.0	17.797483	14-15
AGAGTAC	33120	0.0	16.606062	10-11
TACATGG	28580	0.0	15.979619	2
ACATGGG	29175	0.0	15.441986	3
GTACATG	29150	0.0	15.243306	1
>>END_MODULE