FastQCFastQC Report
Fri 27 May 2016
SRR2140380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10659010
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173750.057.5837551
GTATCAA326300.039.8787571
TCAACGC393550.032.5494354
ATCAACG393850.032.5040253
TATCAAC402300.031.9849952
CAACGCA404050.031.714445
AACGCAG414650.030.9845876
TGGTATC72450.028.9647662
GTGGTAT78300.028.1463741
ACGCAGA473000.027.0880687
CGCAGAG483300.026.5107758
TATCTCG136100.026.11087694-95
GCAGAGT551400.023.0456079
GTACATG462900.022.3254261
GAGTACT271250.022.08911312-13
TACATGG455750.022.0206452
ACATGGG458250.021.2969553
CAGAGTA535100.020.95136310-11
GTACTTT296950.019.7615314-15
CATGGGG320050.019.2715454
Produced by FastQC (version 0.11.5)