##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10659010
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.79922750799558	32.0	18.0	33.0	18.0	33.0
2	29.867608436430775	31.0	29.0	33.0	25.0	33.0
3	31.09361826285931	33.0	31.0	33.0	27.0	33.0
4	30.945171362068336	33.0	31.0	33.0	27.0	34.0
5	31.354545684824387	33.0	32.0	33.0	28.0	34.0
6	34.204232757075935	37.0	34.0	38.0	26.0	38.0
7	35.60886874109322	38.0	36.0	38.0	30.0	38.0
8	35.412131239205145	38.0	35.0	38.0	29.0	38.0
9	36.08398547332257	38.0	37.0	38.0	33.0	38.0
10-11	36.302657188613196	38.0	37.0	38.0	33.0	38.0
12-13	36.39361718395986	38.0	37.0	38.0	33.0	38.0
14-15	36.38370904052065	38.0	37.0	38.0	33.0	38.0
16-17	36.41328228418962	38.0	37.0	38.0	33.5	38.0
18-19	36.37353666053414	38.0	37.0	38.0	33.5	38.0
20-21	36.42343402255425	38.0	37.0	38.0	33.5	38.0
22-23	36.47037001068652	38.0	38.0	38.0	34.0	38.0
24-25	36.47822028137373	38.0	38.0	38.0	34.0	38.0
26-27	36.43231436544624	38.0	38.0	38.0	34.0	38.0
28-29	36.412755106593174	38.0	38.0	38.0	34.0	38.0
30-31	36.40354064837977	38.0	38.0	38.0	34.0	38.0
32-33	36.38015711792898	38.0	38.0	38.0	33.0	38.0
34-35	36.353412916801744	38.0	38.0	38.0	33.0	38.0
36-37	36.31897218402875	38.0	37.0	38.0	33.0	38.0
38-39	36.30654742043727	38.0	37.0	38.0	33.0	38.0
40-41	36.30352713888061	38.0	37.0	38.0	33.0	38.0
42-43	36.2709230935082	38.0	37.0	38.0	33.0	38.0
44-45	36.21488367949013	38.0	37.0	38.0	33.0	38.0
46-47	36.166746774701885	38.0	37.0	38.0	33.0	38.0
48-49	36.11986084501566	38.0	37.0	38.0	33.0	38.0
50-51	36.09489641190943	38.0	37.0	38.0	33.0	38.0
52-53	36.07042017637751	38.0	37.0	38.0	33.0	38.0
54-55	35.98407916153348	38.0	37.0	38.0	31.0	38.0
56-57	36.009307956753204	38.0	37.0	38.0	31.0	38.0
58-59	35.98933964873822	38.0	37.0	38.0	31.0	38.0
60-61	35.98270134978811	38.0	37.0	38.0	31.0	38.0
62-63	35.96740177804724	38.0	37.0	38.0	31.0	38.0
64-65	35.97195942379034	38.0	37.0	38.0	31.0	38.0
66-67	35.96219490352411	38.0	37.0	38.0	31.0	38.0
68-69	35.93854854328579	38.0	37.0	38.0	31.0	38.0
70-71	35.91635968110434	38.0	37.0	38.0	31.0	38.0
72-73	35.90210395698763	38.0	37.0	38.0	31.0	38.0
74-75	35.88573229738629	38.0	37.0	38.0	31.0	38.0
76-77	35.887009440186574	38.0	37.0	38.0	31.0	38.0
78-79	35.84383389098531	38.0	37.0	38.0	31.0	38.0
80-81	35.81857272478065	38.0	37.0	38.0	31.0	38.0
82-83	35.719985662580825	38.0	36.5	38.0	30.0	38.0
84-85	35.741642776886344	38.0	37.0	38.0	30.0	38.0
86-87	35.75160290499738	38.0	37.0	38.0	31.0	38.0
88-89	35.758565292863175	38.0	37.0	38.0	31.0	38.0
90-91	35.742364593631194	38.0	37.0	38.0	31.0	38.0
92-93	35.70953685281371	38.0	36.0	38.0	31.0	38.0
94-95	35.71379121174739	38.0	36.0	38.0	31.0	38.0
96-97	35.68534169722056	38.0	36.0	38.0	31.0	38.0
98-99	35.68615675930215	38.0	36.0	38.0	31.0	38.0
100-101	35.14462345767399	38.0	35.0	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	8.0
19	30.0
20	153.0
21	852.0
22	3637.0
23	11008.0
24	24245.0
25	44917.0
26	71944.0
27	105494.0
28	146855.0
29	191608.0
30	244641.0
31	305178.0
32	382167.0
33	490125.0
34	661745.0
35	990313.0
36	1964460.0
37	5019626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.298962098731494	18.55806496100482	13.721696480254733	21.42127646000895
2	14.957561724775564	20.298320388103587	42.7964886044764	21.94762928264445
3	18.192978551892473	27.391558560478916	29.85671927117265	24.558743616455956
4	12.58626270169556	19.340792437571594	38.109261554309455	29.963683306423395
5	14.165293040250063	37.82296490609389	34.61011052244611	13.40163153120994
6	29.427911222524415	38.03003280792494	18.922460903967632	13.619595065583015
7	26.625962448670187	33.556699918660364	22.395184918674435	17.422152713995015
8	24.77530277202104	36.42142187689101	20.746551509005055	18.056723842082896
9	26.070338614937032	17.29109926719273	20.914128047539123	35.72443407033111
10-11	24.326766744753968	27.041807822677715	28.28212000926915	20.349305423299164
12-13	25.327511654459467	24.573159233362198	28.536341555172573	21.562987557005762
14-15	22.502408760288244	25.820826699665357	26.511936849669905	25.1648276903765
16-17	21.67641774140829	29.09358911477216	27.0672491105166	22.16274403330295
18-19	21.69835191073092	28.019112469169276	29.097317668338807	21.185217951760997
20-21	22.8419001817647	26.94193547055165	29.002475000960693	21.21368934672296
22-23	22.502931794402727	26.91519847756482	28.918435266819692	21.66343446121276
24-25	22.084830261691344	27.127278159054967	29.029261861890255	21.758629717363434
26-27	21.978360939137005	27.260086263988693	29.00006799243143	21.761484804442873
28-29	21.90039389532248	27.212023884208325	29.095482996017264	21.79209922445193
30-31	22.215842113806673	27.035611444281198	28.971555573779955	21.776990868132174
32-33	21.945010480699025	27.345782990649802	28.8558185543701	21.853387974281073
34-35	22.158810441831683	27.280180362893645	28.865899758023062	21.69510943725161
36-37	22.047298169661374	27.16912419073063	28.99316835369932	21.790409285908673
38-39	21.871525484964017	27.303185289765867	29.03742811126671	21.7878611140034
40-41	22.213091293978465	27.090555409279283	28.915045685540917	21.78130761120133
42-43	22.226217066184674	27.13481436411447	28.714011713535502	21.924956856165355
44-45	22.02203465090534	27.363722941638752	28.573235036759705	22.041007370696203
46-47	22.23191520238012	27.20248495362122	28.22780014583962	22.33779969815904
48-49	22.080896618943278	27.423741677113966	28.310581939499897	22.184779764442858
50-51	21.93529375217757	27.599817574611258	28.243951005329077	22.220937667882097
52-53	22.225692180773816	27.55505043584755	27.789272638080153	22.429984745298484
54-55	22.20956290673035	27.300242480715475	27.883323574695645	22.606871037858532
56-57	22.20565236111129	27.228959930568486	28.026510758264216	22.538876950056004
58-59	21.961648684336517	27.155717786202672	28.35244186170096	22.53019166775985
60-61	22.130781912435253	27.038922557036333	28.3850109941118	22.445284536416622
62-63	21.825767117064466	27.54898001080971	28.362364452097832	22.26288842002799
64-65	21.511387423325335	27.74897954632766	28.170002245691446	22.569630784655562
66-67	21.62152262630515	28.183796853380837	27.721404683285105	22.473275837028904
68-69	21.673700683760032	28.30004778739995	27.509544294762527	22.516707234077494
70-71	21.707273667411283	27.815315962176886	27.546386205480506	22.93102416493133
72-73	21.668813728855277	27.549980544912206	27.470757930261218	23.3104477959713
74-75	21.869726176252595	27.400785856392552	27.535857415982072	23.19363055137278
76-77	22.297454042975378	27.236248186303914	27.369189541546223	23.097108229174484
78-79	22.436435189517205	27.184366866632704	26.775440436459665	23.603757507390426
80-81	22.392996367535147	27.44338655489717	26.734959915771405	23.42865716179628
82-83	22.382750592693927	27.555606807738855	26.852369510363484	23.209273089203737
84-85	22.468892471731827	27.541739075659628	26.760249205033087	23.229119247575458
86-87	22.51782268655785	27.923669058461748	26.726049424246902	22.832458830733508
88-89	22.74310214503282	27.681552895329215	26.944560418046915	22.63078454159104
90-91	22.60400470396541	27.63321736552028	27.196485556321637	22.566292374192678
92-93	22.428433820191096	27.293128956865214	27.03053434402854	23.24790287891515
94-95	22.25295816584223	27.346195486341564	27.50595018276635	22.894896165049854
96-97	21.972621336687094	27.543887941442364	27.72676395618031	22.756726765690225
98-99	22.257155802503288	27.793040168407053	27.924895840576507	22.024908188513155
100-101	23.035270754083687	30.566310975609756	25.310611993734618	21.08780627657194
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2500.0
1	2066.5
2	1975.5
3	2797.0
4	4055.5
5	6237.5
6	8092.5
7	8674.0
8	8463.5
9	8114.5
10	8009.5
11	8653.5
12	10741.5
13	16702.5
14	26912.5
15	39491.0
16	50958.0
17	57493.5
18	59599.5
19	54908.0
20	47558.5
21	41913.5
22	38776.5
23	40774.0
24	47173.0
25	59075.5
26	76920.0
27	96895.0
28	117438.5
29	138875.5
30	158685.0
31	179669.5
32	204992.5
33	233257.0
34	259478.5
35	283258.0
36	311081.5
37	335207.5
38	351407.5
39	364483.0
40	381017.0
41	394956.5
42	402358.0
43	412652.5
44	424690.0
45	438928.5
46	445900.5
47	444645.5
48	443073.0
49	437733.5
50	427230.5
51	413960.5
52	397117.0
53	376454.0
54	355597.5
55	332506.5
56	305598.5
57	275292.0
58	244725.5
59	212881.0
60	176980.5
61	141362.0
62	107720.5
63	79871.5
64	58769.5
65	42481.5
66	30780.0
67	22891.0
68	17096.5
69	11976.0
70	8129.0
71	5326.0
72	3403.5
73	2113.5
74	1290.5
75	777.0
76	493.0
77	301.5
78	193.0
79	130.5
80	84.5
81	45.0
82	30.0
83	26.5
84	19.5
85	13.0
86	8.5
87	8.0
88	6.0
89	3.0
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003321133951464536
4	0.0
5	0.050070316098774645
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.3134428056639405E-4
18-19	0.0
20-21	0.0
22-23	4.693311346108339E-6
24-25	0.0
26-27	2.4886670563074067E-4
28-29	1.8791335653374272E-4
30-31	9.404794093789309E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.64268379531359E-5
44-45	0.0
46-47	9.840107508533005E-4
48-49	0.0016394066231536733
50-51	0.0023131328463331974
52-53	0.005742534630272909
54-55	0.00718638625340992
56-57	0.0058989769039845515
58-59	0.007818407775019853
60-61	0.00701213098660683
62-63	0.006259823500193919
64-65	0.006008806482399987
66-67	0.0013216738476758596
68-69	0.001871750160856761
70-71	3.6031235634829376E-4
72-73	0.0018239635814568693
74-75	0.002137196193175103
76-77	6.221949266976602E-4
78-79	0.0
80-81	1.7502924492547828E-4
82-83	0.0
84-85	0.0
86-87	6.760669646582051E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	6.015312863498869E-4
96-97	5.979463294137752E-5
98-99	5.134636669088194E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1269.0
20-21	3745.0
22-23	2330.0
24-25	2587.0
26-27	4148.0
28-29	7944.0
30-31	17266.0
32-33	21977.0
34-35	29886.0
36-37	46118.0
38-39	35197.0
40-41	57947.0
42-43	65613.0
44-45	78411.0
46-47	79333.0
48-49	72462.0
50-51	92008.0
52-53	6422.0
54-55	5781.0
56-57	5764.0
58-59	6469.0
60-61	76910.0
62-63	75829.0
64-65	82085.0
66-67	89299.0
68-69	90542.0
70-71	96408.0
72-73	80644.0
74-75	81311.0
76-77	86945.0
78-79	91381.0
80-81	93792.0
82-83	88076.0
84-85	86175.0
86-87	89943.0
88-89	97471.0
90-91	103598.0
92-93	100697.0
94-95	109923.0
96-97	174260.0
98-99	1012062.0
100-101	7208982.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.59166787141012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.03479564825133	70.14279344139922
2	8.578236511718904	13.826687757497075
3	2.3560387042523776	5.696312662358852
4	0.906866582161642	2.9234356157300767
5	0.42938289122650536	1.7302341679696174
6	0.23668604610595453	1.144495393054101
7	0.12857000487497092	0.7253169791776467
8	0.07925145943910908	0.5109605837952969
9	0.0592235229497759	0.42956302425688575
>10	0.18500574066517575	2.2674528357845785
>50	0.004017351973951906	0.22446239350376526
>100	0.0017939329833656658	0.23994587824653657
>500	8.77356741468117E-5	0.05476305059017492
>1k	4.386772289898791E-5	0.08357621663610865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.381734326171004E-6	0.0	0.0	0.0
3	0.0	9.381734326171004E-6	0.0	1.8763468652342008E-5	0.0
4	0.0	4.690867163085502E-5	0.0	1.8763468652342008E-5	0.0
5	0.0	4.690867163085502E-5	0.0	1.8763468652342008E-5	0.0
6	0.0	4.690867163085502E-5	0.0	1.8763468652342008E-5	2.8145202978513015E-5
7	0.0	4.690867163085502E-5	0.0	1.8763468652342008E-5	2.8145202978513015E-5
8	0.0	5.629040595702603E-5	0.0	2.8145202978513015E-5	3.7526937304684015E-5
9	0.0	1.2196254624022307E-4	0.0	3.7526937304684015E-5	4.690867163085502E-5
10-11	0.0	1.2196254624022307E-4	0.0	7.974474177245355E-5	4.690867163085502E-5
12-13	0.0	1.2196254624022307E-4	0.0	1.1258081191405206E-4	4.690867163085502E-5
14-15	0.0	3.705785058837547E-4	0.0	1.1258081191405206E-4	6.567214028319704E-5
16-17	0.0	3.940328416991822E-4	0.0	1.3134428056639407E-4	6.567214028319704E-5
18-19	0.0	4.315597790038662E-4	0.0	2.157798895019331E-4	9.381734326171004E-5
20-21	0.0	4.784684506347213E-4	0.0	2.4392509248044613E-4	9.381734326171004E-5
22-23	0.0	5.441405909179183E-4	0.0	2.720702954589592E-4	1.0319907758788105E-4
24-25	0.0	5.722857938964313E-4	0.0	3.3305156857907066E-4	1.1258081191405206E-4
26-27	0.0	5.816675282226023E-4	0.0	4.7846845063472123E-4	1.1727167907713756E-4
28-29	0.0	5.816675282226023E-4	0.0	0.001308751938500855	1.2196254624022307E-4
30-31	0.0	6.567214028319703E-4	0.0	0.003982546221459592	1.2665341340330856E-4
32-33	0.0	6.661031371581413E-4	0.0	0.008940792812840968	1.4072601489256508E-4
34-35	0.0	7.786839490721934E-4	0.0	0.015250009147190966	1.4072601489256508E-4
36-37	1.8763468652342008E-5	8.95955628149331E-4	0.0	0.024021930742160857	1.5010774921873606E-4
38-39	1.8763468652342008E-5	9.475551669432715E-4	0.0	0.05142597670890636	1.5479861638182155E-4
40-41	2.345433581542751E-5	0.0010413725102049816	0.0	0.2971758165157927	1.5948948354490707E-4
42-43	2.8145202978513015E-5	0.0010788994475096655	0.0	0.32632955593436913	1.7356208503416356E-4
44-45	2.8145202978513015E-5	0.0011867893922606321	0.0	0.3760105300586077	1.876346865234201E-4
46-47	3.283607014159851E-5	0.0012571523997069146	0.0	0.432042938321664	2.017072880126766E-4
48-49	3.7526937304684015E-5	0.00126184326687	0.0	0.4868885571924597	2.533068268066171E-4
50-51	4.690867163085502E-5	0.001411951016088736	0.0	0.5432211809539536	2.533068268066171E-4
52-53	4.690867163085502E-5	0.0014166418832518217	0.0	0.724621705017633	2.8614289694821565E-4
54-55	5.1599538793940526E-5	0.001430714484741078	0.0	1.3069037368385996	3.2836070141598517E-4
56-57	5.629040595702603E-5	0.0014354053519041636	0.0	1.9482531679771387	3.5650590439449816E-4
58-59	5.629040595702603E-5	0.0014447870862303348	0.0	2.618357614825392	3.846511073730112E-4
60-61	5.629040595702603E-5	0.0014916957578611897	0.0	3.1325094919697047	3.893419745360967E-4
62-63	6.567214028319704E-5	0.001515150093676617	0.0	3.1432750321089857	4.127963103515242E-4
64-65	6.567214028319704E-5	0.0017637660533201488	0.0	3.150513040141627	4.174871775146097E-4
66-67	7.036300744628254E-5	0.0017731477876463198	0.0	3.156747202601367	4.3155977900386617E-4
68-69	7.505387460936803E-5	0.0018247473264402605	0.0	3.1625638778835934	4.4563238049312275E-4
70-71	7.505387460936803E-5	0.0018388199279295169	0.0	3.1678880121136954	5.019227864501487E-4
72-73	7.505387460936803E-5	0.0018482016622556878	0.0	3.1720581930216785	5.253771222655763E-4
74-75	7.505387460936803E-5	0.0018716559980711154	0.0	3.175430926511937	5.347588565917473E-4
76-77	7.505387460936803E-5	0.0019560916070066542	0.0	3.17827828287993	5.394497237548328E-4
78-79	7.505387460936803E-5	0.002007691145800595	0.0	3.1809661497643775	5.441405909179183E-4
80-81	7.505387460936803E-5	0.002007691145800595	0.0	3.18218577522678	5.675949267333457E-4
82-83	7.505387460936803E-5	0.0020217637472898517	0.0	3.182659552810252	6.004309968749442E-4
84-85	7.505387460936803E-5	0.0020405272159421933	0.0	3.183072349120603	6.051218640380298E-4
86-87	7.505387460936803E-5	0.002092126754736134	0.0	3.183292819877268	6.098127312011153E-4
88-89	7.505387460936803E-5	0.0021671806293455022	0.0	3.1833819463533666	6.191944655272863E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17375	0.0	57.583755	1
GTATCAA	32630	0.0	39.878757	1
TCAACGC	39355	0.0	32.549435	4
ATCAACG	39385	0.0	32.504025	3
TATCAAC	40230	0.0	31.984995	2
CAACGCA	40405	0.0	31.71444	5
AACGCAG	41465	0.0	30.984587	6
TGGTATC	7245	0.0	28.964766	2
GTGGTAT	7830	0.0	28.146374	1
ACGCAGA	47300	0.0	27.088068	7
CGCAGAG	48330	0.0	26.510775	8
TATCTCG	13610	0.0	26.110876	94-95
GCAGAGT	55140	0.0	23.045607	9
GTACATG	46290	0.0	22.325426	1
GAGTACT	27125	0.0	22.089113	12-13
TACATGG	45575	0.0	22.020645	2
ACATGGG	45825	0.0	21.296955	3
CAGAGTA	53510	0.0	20.951363	10-11
GTACTTT	29695	0.0	19.76153	14-15
CATGGGG	32005	0.0	19.271545	4
>>END_MODULE