FastQCFastQC Report
Fri 27 May 2016
SRR2140379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11412510
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198100.057.8765221
GTATCAA365750.038.550061
TATCTCG107000.035.79514794-95
TCAACGC465550.030.5767844
ATCAACG465850.030.5309143
TATCAAC467700.030.4665682
GTGGTAT80100.029.6488021
CAACGCA479900.029.6166575
TGGTATC76950.029.2622172
CCGTCTT56000.029.10869692-93
AACGCAG495850.028.7127196
TATGCCG55300.028.0015988-89
TGCCGTC56300.027.69711790-91
TCTCGTA51100.025.47550682-83
ACGCAGA576250.024.706647
GCCGTCT58400.024.6949990-91
ATGCCGT57750.024.57520588-89
CGCAGAG586700.024.2590898
CTATCTC171700.023.48486394-95
CTCGTAT66100.023.1315184-85
Produced by FastQC (version 0.11.5)