##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11412510
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.961092520400857	30.0	18.0	32.0	18.0	33.0
2	27.757410858785665	29.0	25.0	32.0	18.0	33.0
3	30.500697304974977	32.0	29.0	33.0	25.0	33.0
4	30.18568281648822	31.0	29.0	33.0	25.0	33.0
5	31.672034460429828	33.0	32.0	33.0	30.0	33.0
6	35.14985204832241	37.0	35.0	38.0	29.0	38.0
7	35.552316887345555	38.0	36.0	38.0	30.0	38.0
8	35.996962280865475	38.0	37.0	38.0	31.0	38.0
9	36.26530097235402	38.0	37.0	38.0	33.0	38.0
10-11	36.38318148242587	38.0	37.0	38.0	33.0	38.0
12-13	36.45727762779616	38.0	38.0	38.0	33.5	38.0
14-15	36.46634557165777	38.0	38.0	38.0	34.0	38.0
16-17	36.513324150427906	38.0	38.0	38.0	34.0	38.0
18-19	36.46324209135413	38.0	38.0	38.0	34.0	38.0
20-21	36.49873361880002	38.0	38.0	38.0	34.0	38.0
22-23	36.55100551725113	38.0	38.0	38.0	34.0	38.0
24-25	36.57171992977371	38.0	38.0	38.0	34.0	38.0
26-27	36.52559025445696	38.0	38.0	38.0	34.0	38.0
28-29	36.501119166079924	38.0	38.0	38.0	34.0	38.0
30-31	36.48857469182033	38.0	38.0	38.0	34.0	38.0
32-33	36.46525833109375	38.0	38.0	38.0	34.0	38.0
34-35	36.449631156184296	38.0	38.0	38.0	34.0	38.0
36-37	36.422274858209235	38.0	38.0	38.0	34.0	38.0
38-39	36.41795828364738	38.0	38.0	38.0	34.0	38.0
40-41	36.39334549404002	38.0	38.0	38.0	34.0	38.0
42-43	36.3569526724294	38.0	38.0	38.0	33.0	38.0
44-45	36.296774236720594	38.0	38.0	38.0	33.0	38.0
46-47	36.24186157773599	38.0	37.0	38.0	33.0	38.0
48-49	36.18717760553156	38.0	37.0	38.0	33.0	38.0
50-51	36.16279502014826	38.0	37.0	38.0	33.0	38.0
52-53	36.136147651244386	38.0	37.0	38.0	33.0	38.0
54-55	36.054710458391156	38.0	37.0	38.0	32.0	38.0
56-57	36.09213102255444	38.0	37.0	38.0	33.0	38.0
58-59	36.082282318878256	38.0	37.0	38.0	33.0	38.0
60-61	36.08160319675443	38.0	37.0	38.0	33.0	38.0
62-63	36.070269538000176	38.0	37.0	38.0	33.0	38.0
64-65	36.08127730325188	38.0	37.0	38.0	33.0	38.0
66-67	36.07868141777429	38.0	37.0	38.0	33.0	38.0
68-69	36.06287106991357	38.0	37.0	38.0	32.5	38.0
70-71	36.04417805620075	38.0	37.0	38.0	32.0	38.0
72-73	36.03378795026488	38.0	37.0	38.0	31.5	38.0
74-75	36.02360799888821	38.0	37.0	38.0	31.0	38.0
76-77	36.024702129359035	38.0	37.0	38.0	31.5	38.0
78-79	35.98549949060208	38.0	37.0	38.0	31.0	38.0
80-81	35.96375313162566	38.0	37.0	38.0	31.0	38.0
82-83	35.866016746088825	38.0	37.0	38.0	31.0	38.0
84-85	35.88857324092846	38.0	37.0	38.0	31.0	38.0
86-87	35.906619341527474	38.0	37.0	38.0	31.0	38.0
88-89	35.915750531739754	38.0	37.0	38.0	31.0	38.0
90-91	35.90229950814249	38.0	37.0	38.0	31.0	38.0
92-93	35.87206802727734	38.0	37.0	38.0	31.0	38.0
94-95	35.87500209096887	38.0	37.0	38.0	31.0	38.0
96-97	35.85001626310101	38.0	37.0	38.0	31.0	38.0
98-99	35.851775297785885	38.0	37.0	38.0	31.0	38.0
100-101	35.348987515705005	38.0	35.5	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	8.0
19	32.0
20	116.0
21	703.0
22	3051.0
23	9509.0
24	22036.0
25	41745.0
26	68958.0
27	104410.0
28	147465.0
29	197013.0
30	252347.0
31	316823.0
32	397201.0
33	507095.0
34	686080.0
35	1037192.0
36	2127902.0
37	5492822.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.40123513582902	22.538635234492673	13.999050165125816	23.061079464552495
2	15.984818414178825	21.121611284458897	40.09131207771121	22.802258223651066
3	18.235804520478396	27.294234180963194	29.734117959217937	24.735843339340473
4	13.515431749895509	18.491120708766083	37.95739938015388	30.036048161184524
5	13.902068573126828	37.739977232589226	34.86569024836817	13.492263945915784
6	29.901648261714254	38.032008389391706	18.35102328077229	13.715320068121747
7	26.682876948191065	33.56721702763021	22.320532468317662	17.42937355586107
8	25.09575018992316	36.41516195823706	20.39918475427404	18.08990309756574
9	26.16956304967093	16.910171382106125	20.793199743088945	36.127065825134
10-11	24.59028732504944	26.95365436700603	28.022380703280874	20.433677604663654
12-13	25.47430957231624	24.562078130143068	28.433080384292342	21.530531913248353
14-15	22.732615349296516	25.669795689116594	26.470697506508213	25.12689145507868
16-17	21.78291425319354	28.79243758704823	27.129816924344762	22.294831235413465
18-19	21.781036774557045	27.777684313091513	29.238147436453506	21.20313147589794
20-21	23.023256455853716	26.680894836916174	29.15756558702944	21.138283120200672
22-23	22.673493897724466	26.50662804482306	29.057568626365075	21.7623094310874
24-25	22.181080785252217	26.804389870147922	29.158874049188206	21.855655295411662
26-27	22.05803379581346	26.913430931636984	29.1338465353169	21.894688737232656
28-29	21.957546736585503	26.985159301335983	29.17698375862746	21.88031020345106
30-31	22.389921774385233	26.85644602119291	28.949124588241492	21.804507616180366
32-33	22.12813573207296	27.017715419376557	28.959950881332002	21.894197967218474
34-35	22.236324516218545	27.02375137365965	28.966417737882615	21.773506372239193
36-37	22.24872386145669	26.826792319224918	29.06474654211932	21.85973727719907
38-39	22.081816380216907	27.028117926878686	29.088661448028308	21.8014042448761
40-41	22.32953235876063	26.840208751112065	28.973701234713612	21.85655765541369
42-43	22.258630997882413	26.876194091350097	28.78366284367803	22.081512067089463
44-45	22.192946138560636	27.04470157607377	28.649653043681404	22.11269924168419
46-47	22.333767202711737	26.899715075469814	28.382790078320152	22.383727643498297
48-49	22.163678558629627	27.164785836665285	28.437534695513662	22.234000909191426
50-51	22.13684022300077	27.3386375299575	28.2367786578615	22.287743589180227
52-53	22.334345410123102	27.223277193889306	27.86127301736152	22.581104378626073
54-55	22.314186439871232	26.957395753157964	28.08883285561875	22.639584951352056
56-57	22.264995107314967	26.822540857137827	28.322352458909368	22.590111576637838
58-59	22.126823591532236	26.709203380283576	28.470355934490428	22.693617093693756
60-61	22.21648273586119	26.457849734218584	28.719019358880416	22.6066481710398
62-63	22.02457755478104	26.886537727654247	28.688131699602277	22.400753017962437
64-65	21.631893029050985	27.0808843300398	28.55936118885224	22.727861452056974
66-67	21.825562761809195	27.61321930804248	27.979994381902895	22.581223548245433
68-69	21.825347326047158	27.756846564161037	27.732881007932175	22.684925101859633
70-71	21.815428620236002	27.330426764762382	27.787643838073656	23.066500776927956
72-73	21.775434896999364	27.146096436974677	27.647010749118227	23.431457916907732
74-75	21.943749167047653	26.9989212108055	27.715165036449623	23.34216458569723
76-77	22.384317394372022	26.848466530755648	27.599717334881436	23.167498739990897
78-79	22.551971975322626	26.793070104453903	26.972341733003734	23.682616187219736
80-81	22.541052539252547	27.04912669964986	26.904745939415132	23.505074821682463
82-83	22.495814310880736	27.117771093239035	27.010329625427403	23.376084970452826
84-85	22.565014183955114	27.181290724605205	26.869000311024106	23.38469478041558
86-87	22.626371881480132	27.593223483246575	26.82839186117867	22.95201277409462
88-89	22.84696668145839	27.28542548824461	27.084241490476018	22.783366339820986
90-91	22.699618004848983	27.23828663492404	27.361251295928774	22.7008440642982
92-93	22.59058219838394	26.908650673603816	27.1340337934946	23.36673333451764
94-95	22.34822077359023	26.973372586657923	27.657333580242216	23.021073059509625
96-97	22.051506801694217	27.170096561847444	27.869028454107465	22.909368182350875
98-99	22.343954570100905	27.393686992372995	28.058784384733897	22.203574052792202
100-101	23.15206254703029	30.178957715457315	25.408431091234068	21.26054864627832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3349.0
1	2874.0
2	3430.0
3	4640.0
4	5530.5
5	7844.5
6	10601.0
7	14846.5
8	19551.5
9	21706.0
10	20445.5
11	18549.5
12	19812.0
13	24607.5
14	34820.5
15	47090.5
16	55783.0
17	59607.5
18	58188.0
19	52451.5
20	45140.0
21	38265.5
22	34693.5
23	36590.5
24	43691.0
25	56336.5
26	75328.0
27	96141.0
28	115699.0
29	136970.5
30	157365.5
31	178327.0
32	205328.5
33	235809.0
34	263736.0
35	289756.0
36	319953.0
37	347492.0
38	364366.0
39	375723.5
40	392425.0
41	407569.5
42	416073.0
43	429331.5
44	443586.0
45	452209.5
46	456826.5
47	461473.5
48	464980.0
49	462975.5
50	457913.5
51	451004.5
52	436668.5
53	418970.5
54	402526.5
55	379417.0
56	350734.5
57	318037.5
58	284629.5
59	248783.0
60	205525.0
61	163012.0
62	123719.5
63	91107.5
64	66747.0
65	48286.5
66	34926.5
67	25840.0
68	19407.5
69	13745.5
70	9222.0
71	5874.0
72	3513.0
73	2082.0
74	1241.5
75	765.0
76	522.0
77	337.5
78	205.5
79	131.5
80	88.5
81	60.0
82	42.0
83	29.0
84	19.0
85	13.5
86	12.5
87	12.0
88	7.5
89	3.5
90	2.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.004661551227556428
4	0.0
5	0.05130335044613324
6	1.7524628675024162E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.3811571687560406E-6
14-15	0.0
16-17	1.3143471506268123E-4
18-19	0.0
20-21	2.1906114075300428E-5
22-23	0.0
24-25	0.0
26-27	2.936196922926978E-4
28-29	2.455009216148437E-4
30-31	8.775639071654979E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.719226547144468E-5
44-45	4.510026035929303E-6
46-47	9.137358362421727E-4
48-49	0.0015349987504651962
50-51	0.0021785959410726722
52-53	0.0055028212166329405
54-55	0.006811757567180089
56-57	0.005324771818891408
58-59	0.007248412776603779
60-61	0.006851489080264496
62-63	0.005913723610595533
64-65	0.005713488571389756
66-67	0.0012551316630728382
68-69	0.0018534688897894661
70-71	3.983980898657826E-4
72-73	0.00171098141207443
74-75	0.002076928953947315
76-77	6.386067516499254E-4
78-79	0.0
80-81	1.831529598891965E-4
82-83	0.0
84-85	0.0
86-87	4.7163930660846845E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	5.69955112198678E-4
96-97	2.2227118362461507E-5
98-99	5.633123085636289E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	109.0
20-21	382.0
22-23	861.0
24-25	1322.0
26-27	3032.0
28-29	7346.0
30-31	17760.0
32-33	22816.0
34-35	31306.0
36-37	48803.0
38-39	37606.0
40-41	62396.0
42-43	71444.0
44-45	85822.0
46-47	88465.0
48-49	81654.0
50-51	72889.0
52-53	8508.0
54-55	7184.0
56-57	6987.0
58-59	6970.0
60-61	83724.0
62-63	81799.0
64-65	84113.0
66-67	89947.0
68-69	94131.0
70-71	94191.0
72-73	88109.0
74-75	88308.0
76-77	92754.0
78-79	98454.0
80-81	101139.0
82-83	94819.0
84-85	92791.0
86-87	95897.0
88-89	102938.0
90-91	107454.0
92-93	106840.0
94-95	117324.0
96-97	186269.0
98-99	1092934.0
100-101	7754913.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.3019522218023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.65557773536476	68.7195648532257
2	8.718476101450257	13.827843504882667
3	2.39952996122314	5.708622310191016
4	0.9562083371381462	3.0331675146327317
5	0.4648684870790782	1.8432489275883284
6	0.24762310672733465	1.1782197467223203
7	0.16250241714963232	0.9020731244509271
8	0.09206887035779637	0.5840992926583422
9	0.0674549280107929	0.481437673041331
>10	0.22668708958011025	2.7560438369841482
>50	0.005799517504126473	0.31033384726786684
>100	0.0029088977701344897	0.3980629472581532
>500	1.5860603177848289E-4	0.09158347403488057
>1k	1.3594461294394415E-4	0.1656989470615873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7524628675024162E-5	0.0	0.0	0.0
3	0.0	1.7524628675024162E-5	0.0	0.0	0.0
4	0.0	5.2573886025072483E-5	0.0	0.0	8.762314337512081E-6
5	0.0	6.133620036258457E-5	0.0	0.0	8.762314337512081E-6
6	0.0	6.133620036258457E-5	0.0	0.0	2.6286943012536242E-5
7	0.0	6.133620036258457E-5	0.0	0.0	2.6286943012536242E-5
8	0.0	8.762314337512081E-5	0.0	0.0	3.5049257350048325E-5
9	0.0	1.7524628675024163E-4	0.0	0.0	3.5049257350048325E-5
10-11	0.0	1.7524628675024163E-4	0.0	0.0	3.5049257350048325E-5
12-13	0.0	1.7524628675024163E-4	0.0	0.0	3.5049257350048325E-5
14-15	0.0	2.365824871128262E-4	0.0	0.0	5.2573886025072483E-5
16-17	0.0	2.4534480145033827E-4	0.0	4.3811571687560406E-6	9.63854577126329E-5
18-19	0.0	2.7163174446287453E-4	0.0	1.3143471506268121E-5	1.1391008638765705E-4
20-21	0.0	3.0668100181292286E-4	0.0	3.9430414518804366E-5	1.182912435564131E-4
22-23	0.0	3.9430414518804366E-4	0.0	6.57173575313406E-5	1.621028152439735E-4
24-25	0.0	4.994519172381887E-4	0.0	1.182912435564131E-4	2.2343901560655806E-4
26-27	0.0	5.257388602507249E-4	0.0	2.497259586190943E-4	2.365824871128262E-4
28-29	0.0	5.257388602507249E-4	0.0	9.156618482700124E-4	2.4096364428158223E-4
30-31	0.0	5.520258032632611E-4	0.0	0.003036141917947936	2.4972595861909427E-4
32-33	0.0	5.520258032632611E-4	0.0	0.0071062369277222984	2.7163174446287453E-4
34-35	0.0	6.615547324821621E-4	0.0	0.012534490659811031	3.1544331615043493E-4
36-37	0.0	7.754648188698192E-4	0.0	0.020709729936709803	3.1544331615043493E-4
38-39	0.0	8.061329190511115E-4	0.0	0.04702734104942734	3.24205630487947E-4
40-41	0.0	8.67469119413696E-4	0.0	0.29882558700934325	3.3296794482545907E-4
42-43	0.0	9.112806911012565E-4	0.0	0.3250993865503732	3.373491019942151E-4
44-45	0.0	0.0010865269778514981	0.0	0.37199529288473787	3.504925735004832E-4
46-47	0.0	0.0012091993785766672	0.0	0.42230850181073226	3.855418308505316E-4
48-49	0.0	0.0012267240072516914	0.0	0.4720784472478009	4.2059108820057987E-4
50-51	0.0	0.0014983557517145659	0.0	0.5235920932380345	4.2497224536933593E-4
52-53	0.0	0.0015334050090646141	0.0	0.7031932502140195	4.512591883818722E-4
54-55	0.0	0.0015421673234021264	0.0	1.2939221959060716	4.775461313944084E-4
56-57	0.0	0.0015772165807521746	0.0	1.9413827457763453	4.863084457319205E-4
58-59	0.0	0.0015991223665959547	0.0	2.617250718728833	4.906896029006765E-4
60-61	0.0	0.0016254093096084912	0.0	3.1367201430710683	5.082142315757007E-4
62-63	0.0	0.0016692208812960514	0.0	3.146840616130895	5.257388602507249E-4
64-65	0.0	0.0017962744391899765	0.0	3.1537409386716857	5.257388602507249E-4
66-67	0.0	0.0018094179106962448	0.0	3.1595372096059497	5.301200174194809E-4
68-69	0.0	0.001848848325215049	0.0	3.1651801400393076	5.345011745882369E-4
70-71	0.0	0.0018882787397338533	0.0	3.1704769590563338	5.432634889257491E-4
72-73	0.0	0.0019101845255776336	0.0	3.174744206138702	6.045996892883336E-4
74-75	0.0	0.001953996097265194	0.0	3.177959975500569	6.045996892883336E-4
76-77	0.0	0.002059143869315339	0.0	3.180943543532492	6.045996892883336E-4
78-79	0.0	0.0021029554410028997	0.0	3.1839008246214022	6.045996892883336E-4
80-81	0.0	0.0021029554410028997	0.0	3.1852064094576917	6.265054751321138E-4
82-83	0.0	0.0021160989125091675	0.0	3.1857277671607735	6.966039898322104E-4
84-85	0.0	0.002142385855521704	0.0	3.186095784362949	7.053663041697225E-4
86-87	0.0	0.0021818162700405082	0.0	3.186279792964037	7.185097756759907E-4
88-89	0.0	0.002282582884921897	0.0	3.186310461064218	7.272720900135027E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19810	0.0	57.876522	1
GTATCAA	36575	0.0	38.55006	1
TATCTCG	10700	0.0	35.795147	94-95
TCAACGC	46555	0.0	30.576784	4
ATCAACG	46585	0.0	30.530914	3
TATCAAC	46770	0.0	30.466568	2
GTGGTAT	8010	0.0	29.648802	1
CAACGCA	47990	0.0	29.616657	5
TGGTATC	7695	0.0	29.262217	2
CCGTCTT	5600	0.0	29.108696	92-93
AACGCAG	49585	0.0	28.712719	6
TATGCCG	5530	0.0	28.00159	88-89
TGCCGTC	5630	0.0	27.697117	90-91
TCTCGTA	5110	0.0	25.475506	82-83
ACGCAGA	57625	0.0	24.70664	7
GCCGTCT	5840	0.0	24.69499	90-91
ATGCCGT	5775	0.0	24.575205	88-89
CGCAGAG	58670	0.0	24.259089	8
CTATCTC	17170	0.0	23.484863	94-95
CTCGTAT	6610	0.0	23.13151	84-85
>>END_MODULE