FastQCFastQC Report
Fri 27 May 2016
SRR2140378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10653766
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179600.058.30451
GTATCAA347850.041.948681
TATCTCG111450.035.53981894-95
ATCAACG471700.030.8140473
TCAACGC474300.030.6349034
TATCAAC478500.030.5863952
CAACGCA481550.030.1918435
CCGTCTT55300.029.38513892-93
AACGCAG496700.029.3178636
GTGGTAT74100.027.6256141
TGGTATC72100.027.0572642
TATGCCG58000.027.02133888-89
TGCCGTC59150.026.74130890-91
GCCGTCT56900.026.26262590-91
ACGCAGA566450.025.6614917
CGCAGAG575950.025.2458118
TCTCGTA55500.024.66416482-83
ATGCCGT61550.023.92784388-89
CTATCTC176200.023.63915694-95
GCAGAGT644250.022.5083129
Produced by FastQC (version 0.11.5)