FastQCFastQC Report
Fri 27 May 2016
SRR2140377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10423471
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170100.056.4574621
GTATCAA331300.041.7980731
TCAACGC413750.033.4888574
ATCAACG411150.033.1786773
TATCAAC418650.032.7520332
CAACGCA422650.032.7421265
AACGCAG435100.031.850176
ACGCAGA502300.027.5891087
CGCAGAG511600.027.0361378
GTGGTAT74800.026.9332921
TGGTATC73650.026.2215462
GCAGAGT574650.024.0544919
TATCTCG158200.023.76207294-95
GAGTACT305500.022.2572612-13
CAGAGTA560900.020.71799510-11
GTACTTT335750.020.10212914-15
AGAGTAC521450.020.01816410-11
GTACATG427350.019.411381
TACATGG420050.019.3098682
AGTACTT330550.018.7855512-13
Produced by FastQC (version 0.11.5)