FastQCFastQC Report
Fri 27 May 2016
SRR2140376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10162741
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133900.051.6885641
GTATCAA232950.035.1385731
TATCTCG94300.030.75507494-95
TCAACGC286900.028.2205624
ATCAACG287850.028.0977083
CAACGCA296700.027.2884375
TATCAAC308150.026.5634632
AACGCAG306050.026.5601376
CCGTCTT52200.025.50795492-93
TGCCGTC51750.024.91989390-91
GTGGTAT66550.024.3329771
TGGTATC64150.023.8601342
GCCGTCT51450.023.4119690-91
TATGCCG54800.023.39021388-89
ACGCAGA352350.023.0952177
CGCAGAG355600.022.8342558
TCTCGTA49150.022.54642582-83
ATGCCGT55550.021.36700488-89
CGTATGC62750.020.25282786-87
CTCGTAT62550.019.94336184-85
Produced by FastQC (version 0.11.5)