##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140376_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10162741
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.825036670717083	30.0	18.0	32.0	18.0	33.0
2	30.453486515104537	32.0	29.0	33.0	25.0	33.0
3	31.013627721103983	33.0	31.0	33.0	27.0	33.0
4	31.50458542631363	33.0	31.0	33.0	29.0	34.0
5	31.98348555768567	33.0	32.0	34.0	30.0	34.0
6	35.63180415598508	38.0	36.0	38.0	29.0	38.0
7	36.05371493773186	38.0	37.0	38.0	31.0	38.0
8	36.18894892627885	38.0	37.0	38.0	33.0	38.0
9	36.43094003871593	38.0	37.0	38.0	34.0	38.0
10-11	36.47981823998073	38.0	38.0	38.0	34.0	38.0
12-13	36.51407814092674	38.0	38.0	38.0	34.0	38.0
14-15	36.502275419593985	38.0	38.0	38.0	34.0	38.0
16-17	36.512035089745964	38.0	38.0	38.0	34.0	38.0
18-19	36.46072447384027	38.0	38.0	38.0	34.0	38.0
20-21	36.48820231160161	38.0	38.0	38.0	34.0	38.0
22-23	36.527159306006695	38.0	38.0	38.0	34.0	38.0
24-25	36.53208853562374	38.0	38.0	38.0	34.0	38.0
26-27	36.48116445563406	38.0	38.0	38.0	34.0	38.0
28-29	36.46158322470886	38.0	38.0	38.0	34.0	38.0
30-31	36.45219828712417	38.0	38.0	38.0	34.0	38.0
32-33	36.42985656958258	38.0	38.0	38.0	34.0	38.0
34-35	36.40485184448837	38.0	38.0	38.0	34.0	38.0
36-37	36.37055298302394	38.0	38.0	38.0	33.5	38.0
38-39	36.365885103138574	38.0	38.0	38.0	33.0	38.0
40-41	36.348853682895395	38.0	38.0	38.0	33.0	38.0
42-43	36.317157470691335	38.0	38.0	38.0	33.0	38.0
44-45	36.26335809189267	38.0	37.0	38.0	33.0	38.0
46-47	36.22251236312689	38.0	37.0	38.0	33.0	38.0
48-49	36.18076230160453	38.0	37.0	38.0	33.0	38.0
50-51	36.15179363263681	38.0	37.0	38.0	33.0	38.0
52-53	36.116220535738904	38.0	37.0	38.0	33.0	38.0
54-55	36.03197290307736	38.0	37.0	38.0	31.0	38.0
56-57	36.06202733974783	38.0	37.0	38.0	32.5	38.0
58-59	36.046125668526905	38.0	37.0	38.0	32.0	38.0
60-61	36.03954426782407	38.0	37.0	38.0	31.5	38.0
62-63	36.023852673941946	38.0	37.0	38.0	31.0	38.0
64-65	36.025650364983804	38.0	37.0	38.0	31.0	38.0
66-67	36.017018922547706	38.0	37.0	38.0	31.0	38.0
68-69	35.9996184418562	38.0	37.0	38.0	31.0	38.0
70-71	35.97985508129545	38.0	37.0	38.0	31.0	38.0
72-73	35.96626083634027	38.0	37.0	38.0	31.0	38.0
74-75	35.95195726559814	38.0	37.0	38.0	31.0	38.0
76-77	35.95137068270522	38.0	37.0	38.0	31.0	38.0
78-79	35.91265802559557	38.0	37.0	38.0	31.0	38.0
80-81	35.8933295291689	38.0	37.0	38.0	31.0	38.0
82-83	35.79876496431467	38.0	37.0	38.0	30.0	38.0
84-85	35.818046702095245	38.0	37.0	38.0	31.0	38.0
86-87	35.83012760691138	38.0	37.0	38.0	31.0	38.0
88-89	35.83695516183684	38.0	37.0	38.0	31.0	38.0
90-91	35.82073054139414	38.0	37.0	38.0	31.0	38.0
92-93	35.79100689433626	38.0	37.0	38.0	31.0	38.0
94-95	35.79467618629459	38.0	37.0	38.0	31.0	38.0
96-97	35.76998917368083	38.0	37.0	38.0	31.0	38.0
98-99	35.76149469376554	38.0	37.0	38.0	31.0	38.0
100-101	35.195091455815586	38.0	35.5	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	22.0
20	98.0
21	719.0
22	3212.0
23	9342.0
24	21122.0
25	39796.0
26	64112.0
27	95919.0
28	133066.0
29	175391.0
30	222168.0
31	277859.0
32	347708.0
33	444248.0
34	594348.0
35	882651.0
36	1739793.0
37	5111161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.22087554922437	20.68773572011724	17.03281624514489	23.058572485513505
2	13.905628412649698	22.507805718949246	41.37076798473955	22.2157978836615
3	18.821038780616906	26.088248164616758	31.03122427075946	24.05948878400688
4	12.549124296289751	19.513967737640858	39.13460945231213	28.802298513757265
5	13.23004284409161	37.81352384777746	36.25141763538418	12.705015672746747
6	28.82293271077163	39.370087262875245	18.87784014174916	12.929139884603966
7	25.393031269811956	33.339076534568775	23.590141675361007	17.677750520258265
8	23.41324058145337	37.892739763809786	21.353323871975093	17.340695782761756
9	24.515010271343137	17.656329134039726	22.031005217982038	35.7976553766351
10-11	23.388739317473505	27.735494784330328	29.279502449191614	19.596263449004557
12-13	24.207898243200336	25.13834604266703	29.611105901449225	21.042649812683408
14-15	21.29438800024521	26.711578106733214	27.38491023238711	24.60912366063447
16-17	20.746675388732918	29.845008666224953	28.155974239007346	21.25234170603478
18-19	20.770784181157424	28.87944797569868	30.001153232184112	20.34861461095978
20-21	21.523665906113738	27.96351313952559	30.05928868826145	20.453532266099224
22-23	21.166919666500757	27.924199832671754	29.931676268064493	20.977204232762997
24-25	20.96125829461859	28.036518194267074	30.030016686543785	20.97220682457055
26-27	20.996419353865548	28.171073160843342	29.852245011532506	20.980262473758604
28-29	20.918201251317043	28.220939231624403	29.931458804418366	20.929400712640184
30-31	21.189520535828688	28.050723287249873	29.91041195995581	20.849344216965633
32-33	20.976438643332163	28.19802988091557	29.817270093413136	21.008261382339136
34-35	21.118703085541153	28.24122231738343	29.810105527461612	20.829969069613803
36-37	21.073386883214802	28.143331238647345	29.8271356219054	20.95614625623245
38-39	20.963408478996296	28.226253940312578	29.872598177246807	20.937739403444315
40-41	21.18507179299049	28.106120987671453	29.740454105131164	20.96835311420689
42-43	21.146720450309278	28.174346991963144	29.60657019341127	21.072362364316305
44-45	21.135382259872898	28.26964206214379	29.425521154542235	21.16945452344108
46-47	21.14453187024446	28.240680869462125	29.23561679210626	21.37917046818715
48-49	21.119736592005665	28.390487378733418	29.29818703990572	21.191588989355196
50-51	21.095582010094695	28.50382659611132	29.14419448116844	21.25639691262555
52-53	21.170326197235802	28.435624978357062	28.937384581749846	21.456664242657293
54-55	21.19432024501699	28.23189701971026	29.057555986977885	21.516226748294862
56-57	21.197480449564406	28.096046927773166	29.200354334744897	21.506118287917523
58-59	21.08051426724995	27.96471610492477	29.380043663418835	21.574725964406444
60-61	21.16506322487385	27.777575395079566	29.559560242116127	21.497801137930452
62-63	20.92452936448206	28.22520625126884	29.507235028805017	21.343029355444084
64-65	20.64483937002631	28.357404945826055	29.391564820576495	21.606190863571147
66-67	20.743138072629115	28.757533532155833	29.001548259510145	21.49778013570491
68-69	20.83190532246555	28.91996660684376	28.717825960471167	21.530302110219527
70-71	20.79660013712147	28.6017971830003	28.693733833139586	21.907868846738648
72-73	20.766711438483572	28.332146046922524	28.716793187807827	22.184349326786077
74-75	21.001674181629124	28.1353706372481	28.735437070595715	22.12751811052706
76-77	21.306590931663955	28.03623063763511	28.612857262685687	22.044321168015244
78-79	21.453538602509138	28.013278030642407	28.08996164484211	22.443221722006353
80-81	21.420903100059338	28.265380303623143	28.042021991149426	22.271694605168094
82-83	21.36789567707447	28.376188590932095	28.12757000672833	22.128345725265106
84-85	21.464677134661027	28.387261342205385	28.017894837253493	22.130166685880095
86-87	21.572777685128226	28.6457637740861	27.999089112647173	21.782369428138505
88-89	21.768704275242953	28.434709622614886	28.183548005959324	21.613038096182837
90-91	21.586698193143363	28.458443379331104	28.366984136616928	21.58787429090861
92-93	21.406755577613342	28.121164993436143	28.31673770082272	22.15534172812779
94-95	21.260786608281222	28.209789996427588	28.72461262625668	21.80481076903451
96-97	21.009045912645394	28.40630520356937	28.880333333394148	21.704315550391083
98-99	21.334546665259456	28.599287860216027	29.137840981985175	20.928324492539346
100-101	21.953201227225073	31.385101553029678	26.66372530656315	19.9979719131821
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2141.0
1	1959.0
2	2296.5
3	3033.0
4	3786.5
5	5292.5
6	6926.0
7	9664.0
8	12469.5
9	13700.0
10	13560.5
11	12767.0
12	13608.0
13	17330.5
14	25658.5
15	35911.5
16	42923.5
17	45765.5
18	45787.0
19	43878.5
20	40818.5
21	38287.5
22	39101.0
23	44862.5
24	55602.0
25	71240.5
26	91436.5
27	114640.0
28	139937.5
29	166167.0
30	191619.5
31	217773.0
32	245382.5
33	272887.0
34	298708.5
35	321166.0
36	343305.0
37	363860.5
38	379254.0
39	390894.5
40	402760.0
41	412611.0
42	418023.5
43	421548.0
44	423619.5
45	423034.0
46	418240.0
47	412222.5
48	401453.0
49	384943.0
50	367251.5
51	346117.5
52	320583.0
53	294259.0
54	268913.5
55	242922.0
56	216840.0
57	190479.0
58	164022.0
59	138616.0
60	112260.0
61	87528.0
62	66019.0
63	48253.0
64	35093.5
65	25395.0
66	18287.0
67	13384.5
68	9881.0
69	7021.0
70	4949.5
71	3457.5
72	2326.5
73	1492.0
74	951.5
75	586.0
76	358.0
77	209.5
78	123.5
79	82.5
80	59.5
81	44.5
82	29.0
83	17.0
84	12.5
85	10.5
86	8.5
87	5.5
88	4.0
89	2.5
90	1.5
91	2.5
92	2.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.004152423052009295
4	0.0
5	0.0490123678247827
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.1315844809977937E-4
18-19	0.0
20-21	4.920018700007074E-6
22-23	0.0
24-25	0.0
26-27	2.6575364214136214E-4
28-29	1.9199039693571513E-4
30-31	4.926236019711644E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.153537032927213E-4
44-45	0.0
46-47	9.092074113915155E-4
48-49	0.001830665955877371
50-51	0.0022587875970436124
52-53	0.005540285889088232
54-55	0.007121478300979738
56-57	0.0056768585165401
58-59	0.008010187176896777
60-61	0.007248926528419553
62-63	0.006263498322098129
64-65	0.006200661614275722
66-67	0.0013934724241344337
68-69	0.0020294833731103173
70-71	3.823274880486312E-4
72-73	0.0017967754665780763
74-75	0.0021443769622143277
76-77	7.279472114741245E-4
78-79	0.0
80-81	1.796384125757667E-4
82-83	0.0
84-85	0.0
86-87	6.343222667356272E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	5.885917701657247E-4
96-97	2.4326282096932813E-5
98-99	5.65057556448779E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	102.0
20-21	406.0
22-23	801.0
24-25	1219.0
26-27	2377.0
28-29	5088.0
30-31	13055.0
32-33	17426.0
34-35	24973.0
36-37	38236.0
38-39	28870.0
40-41	47975.0
42-43	54975.0
44-45	66258.0
46-47	67220.0
48-49	61899.0
50-51	55493.0
52-53	6909.0
54-55	5989.0
56-57	5644.0
58-59	5486.0
60-61	65029.0
62-63	63790.0
64-65	68664.0
66-67	74042.0
68-69	75740.0
70-71	76295.0
72-73	70900.0
74-75	72321.0
76-77	77222.0
78-79	80976.0
80-81	82153.0
82-83	77747.0
84-85	77130.0
86-87	80485.0
88-89	85162.0
90-91	88581.0
92-93	88420.0
94-95	95866.0
96-97	161208.0
98-99	950399.0
100-101	7140210.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.56981084958068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.5575578028304	72.29610986230932
2	8.559598094144853	14.135287911639436
3	2.1699879967567024	5.3752649531418415
4	0.8122238924377936	2.6826069266439507
5	0.3626840528312751	1.4973376820218853
6	0.19470909748622173	0.9646256010077934
7	0.11324471740808584	0.6545416427269904
8	0.0658502547339582	0.4349794462223705
9	0.04004902360325798	0.29761562732682634
>10	0.12227047338745965	1.4683725014830917
>50	0.0012774485773108793	0.0676011179365561
>100	4.88430455896576E-4	0.07651373469002812
>500	3.914421933835284E-5	0.02514562610843864
>1k	1.9571127458399134E-5	0.02399736674144281
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	9.839865052154729E-6	0.0	0.0	0.0
2	0.0	1.9679730104309457E-5	0.0	1.9679730104309457E-5	0.0
3	0.0	1.9679730104309457E-5	0.0	1.9679730104309457E-5	0.0
4	0.0	5.9039190312928375E-5	0.0	2.9519595156464188E-5	0.0
5	0.0	5.9039190312928375E-5	0.0	2.9519595156464188E-5	0.0
6	0.0	5.9039190312928375E-5	0.0	2.9519595156464188E-5	9.839865052154729E-6
7	0.0	5.9039190312928375E-5	0.0	2.9519595156464188E-5	9.839865052154729E-6
8	0.0	6.88790553650831E-5	0.0	2.9519595156464188E-5	9.839865052154729E-6
9	1.9679730104309457E-5	8.855878546939256E-5	0.0	2.9519595156464188E-5	9.839865052154729E-6
10-11	1.9679730104309457E-5	8.855878546939256E-5	0.0	5.4119257786851E-5	9.839865052154729E-6
12-13	1.9679730104309457E-5	8.855878546939256E-5	0.0	9.839865052154729E-5	9.839865052154729E-6
14-15	1.9679730104309457E-5	1.1315844809977939E-4	0.0	1.1315844809977939E-4	1.4759797578232092E-5
16-17	1.9679730104309457E-5	1.2791824567801147E-4	0.0	1.229983131519341E-4	1.9679730104309457E-5
18-19	3.9359460208618915E-5	1.3283817820408883E-4	0.0	1.4267804325624356E-4	2.9519595156464188E-5
20-21	5.9039190312928375E-5	1.377581107301662E-4	0.0	1.6235777336055302E-4	3.9359460208618915E-5
22-23	5.9039190312928375E-5	1.7711757093878512E-4	0.0	2.0171723356917194E-4	6.88790553650831E-5
24-25	5.9039190312928375E-5	1.9187736851701721E-4	0.0	2.459966263038682E-4	7.871892041723783E-5
26-27	7.871892041723783E-5	1.9679730104309458E-4	0.0	3.2471554672110605E-4	7.871892041723783E-5
28-29	7.871892041723783E-5	1.9679730104309458E-4	0.0	7.97029069224533E-4	7.871892041723783E-5
30-31	7.871892041723783E-5	2.459966263038682E-4	0.0	0.0026223240363992353	7.871892041723783E-5
32-33	7.871892041723783E-5	2.459966263038682E-4	0.0	0.006499230866948198	7.871892041723783E-5
34-35	7.871892041723783E-5	2.9027601903856446E-4	0.0	0.011940676240789764	7.871892041723783E-5
36-37	9.839865052154729E-5	3.3947534429933817E-4	0.0	0.019704329766939845	7.871892041723783E-5
38-39	1.1315844809977939E-4	3.443952768254155E-4	0.0	0.04394483732292302	7.871892041723783E-5
40-41	1.1807838062585675E-4	3.8375473703403443E-4	0.0	0.2493667800842312	7.871892041723783E-5
42-43	1.1807838062585675E-4	4.280341297687307E-4	0.0	0.2742419589360784	7.871892041723783E-5
44-45	1.1807838062585675E-4	4.919932526077364E-4	0.0	0.31605646547520994	8.855878546939256E-5
46-47	1.1807838062585675E-4	5.116729827120459E-4	0.0	0.3643111636909767	1.0331858304762466E-4
48-49	1.229983131519341E-4	5.215128477642007E-4	0.0	0.4103518922700087	1.1807838062585675E-4
50-51	1.2791824567801147E-4	6.002317681814385E-4	0.0	0.45841471311725845	1.1807838062585675E-4
52-53	1.2791824567801147E-4	6.100716332335932E-4	0.0	0.6174072526299745	1.3283817820408883E-4
54-55	1.2791824567801147E-4	6.149915657596705E-4	0.0	1.1376015584771864	1.6235777336055302E-4
56-57	1.2791824567801147E-4	6.19911498285748E-4	0.0	1.7131155856476123	1.7711757093878512E-4
58-59	1.2791824567801147E-4	6.19911498285748E-4	0.0	2.313175156190638	1.7711757093878512E-4
60-61	1.2791824567801147E-4	6.248314308118253E-4	0.0	2.773789079147053	1.8695743599093985E-4
62-63	1.3283817820408883E-4	6.592709584943669E-4	0.0	2.7830237924985	1.9679730104309458E-4
64-65	1.377581107301662E-4	7.281500138594499E-4	0.0	2.7894984237028178	2.066371660952493E-4
66-67	1.377581107301662E-4	7.281500138594499E-4	0.0	2.795343303543798	2.066371660952493E-4
68-69	1.377581107301662E-4	7.379898789116046E-4	0.0	2.800568271886492	2.066371660952493E-4
70-71	1.377581107301662E-4	7.379898789116046E-4	0.0	2.805153649000796	2.066371660952493E-4
72-73	1.4759797578232093E-4	7.42909811437682E-4	0.0	2.8086320412967325	2.1647703114740404E-4
74-75	1.5743784083447566E-4	7.724294065941462E-4	0.0	2.8115397214196447	2.1647703114740404E-4
76-77	1.5743784083447566E-4	7.921091366984557E-4	0.0	2.8141915650512006	2.1647703114740404E-4
78-79	1.6235777336055302E-4	7.97029069224533E-4	0.0	2.816666291111817	2.1647703114740404E-4
80-81	1.672777058866304E-4	7.97029069224533E-4	0.0	2.817817555322919	2.2631689619955877E-4
82-83	1.672777058866304E-4	7.97029069224533E-4	0.0	2.8184079472260484	2.5583649135602293E-4
84-85	1.672777058866304E-4	7.97029069224533E-4	0.0	2.818816301625713	2.5583649135602293E-4
86-87	1.672777058866304E-4	8.363885294331519E-4	0.0	2.8189934191966515	2.6567635640817767E-4
88-89	1.672777058866304E-4	8.855878546939256E-4	0.0	2.819062298252017	2.6567635640817767E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13390	0.0	51.688564	1
GTATCAA	23295	0.0	35.138573	1
TATCTCG	9430	0.0	30.755074	94-95
TCAACGC	28690	0.0	28.220562	4
ATCAACG	28785	0.0	28.097708	3
CAACGCA	29670	0.0	27.288437	5
TATCAAC	30815	0.0	26.563463	2
AACGCAG	30605	0.0	26.560137	6
CCGTCTT	5220	0.0	25.507954	92-93
TGCCGTC	5175	0.0	24.919893	90-91
GTGGTAT	6655	0.0	24.332977	1
TGGTATC	6415	0.0	23.860134	2
GCCGTCT	5145	0.0	23.41196	90-91
TATGCCG	5480	0.0	23.390213	88-89
ACGCAGA	35235	0.0	23.095217	7
CGCAGAG	35560	0.0	22.834255	8
TCTCGTA	4915	0.0	22.546425	82-83
ATGCCGT	5555	0.0	21.367004	88-89
CGTATGC	6275	0.0	20.252827	86-87
CTCGTAT	6255	0.0	19.943361	84-85
>>END_MODULE