FastQCFastQC Report
Fri 27 May 2016
SRR2140375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9137163
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133700.052.613931
GTATCAA252100.039.1396371
CTTATAC64950.034.586811
TCAACGC309700.031.1811524
ATCAACG314050.030.7492543
CAACGCA319400.030.2342015
TATCAAC322950.030.1688022
AACGCAG327350.029.4850886
TATACAC95100.026.3644623
ACGCAGA375000.025.714297
GTGGTAT60700.025.3067361
CGCAGAG382100.025.2700678
TGGTATC59000.024.4403972
TTATACA103250.023.1246132
GCAGAGT438250.022.051919
GAGTACT265800.020.25583612-13
TACACAT128300.020.1090935
GTACATG300050.020.0788751
ACATCTC117250.019.6300478
ACACATC115550.019.4756286
Produced by FastQC (version 0.11.5)