FastQCFastQC Report
Fri 27 May 2016
SRR2140374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10035604
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC170000.059.5948071
GGTATCA138200.052.5152661
TATACAC216100.048.597233
TTATACA221700.046.1541982
GTATCAA265550.038.866491
TACACAT302200.034.6675875
ACACATC316550.032.3346526
ACATCTC324150.031.4578258
TCAACGC329550.030.8430164
ATCAACG333550.030.4857883
ATACACA353000.030.4674764
CACATCT336450.030.2953957
TATCAAC339950.030.1865622
CAACGCA337300.030.1468585
AACGCAG346650.029.3200936
CATCTCC356450.028.5480699
ACGCAGA397700.025.5659357
CGCAGAG404500.025.1151668
GTGGTAT64750.023.5903261
TGGTATC60550.023.4147932
Produced by FastQC (version 0.11.5)