##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10035604
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.303727408933234	33.0	31.0	34.0	30.0	34.0
2	32.41274944686937	34.0	31.0	34.0	31.0	34.0
3	32.50618188999885	34.0	31.0	34.0	31.0	34.0
4	36.04419245717547	37.0	35.0	37.0	35.0	37.0
5	36.09867667157851	37.0	35.0	37.0	35.0	37.0
6	36.09071432073247	37.0	35.0	37.0	35.0	37.0
7	36.048672904989076	37.0	35.0	37.0	35.0	37.0
8	36.04098258560222	37.0	35.0	37.0	35.0	37.0
9	37.78334129166515	39.0	38.0	39.0	35.0	39.0
10-11	37.72774912202594	39.0	37.5	39.0	35.0	39.0
12-13	37.707569818418506	39.0	37.0	39.0	35.0	39.0
14-15	39.05144144786901	40.0	38.0	41.0	36.0	41.0
16-17	39.057816001906815	40.0	38.0	41.0	36.0	41.0
18-19	39.103906700583245	40.0	38.5	41.0	36.0	41.0
20-21	39.134118183090166	40.0	39.0	41.0	36.0	41.0
22-23	39.08974038056742	40.0	39.0	41.0	36.0	41.0
24-25	39.05050085022947	40.0	39.0	41.0	36.0	41.0
26-27	38.99664858868181	40.0	38.5	41.0	35.0	41.0
28-29	38.96838566263135	40.0	38.0	41.0	35.0	41.0
30-31	38.89188271597668	40.0	38.0	41.0	35.0	41.0
32-33	38.7806398566984	40.0	38.0	41.0	35.0	41.0
34-35	38.68994101237739	40.0	38.0	41.0	35.0	41.0
36-37	38.6383882268343	40.0	38.0	41.0	35.0	41.0
38-39	38.561917777001355	40.0	38.0	41.0	34.5	41.0
40-41	38.55188524625883	40.0	38.0	41.0	34.5	41.0
42-43	38.499452259029155	40.0	38.0	41.0	34.0	41.0
44-45	38.46127873873587	40.0	38.0	41.0	34.0	41.0
46-47	38.39109349248191	40.0	38.0	41.0	34.0	41.0
48-49	38.32625740349383	40.0	38.0	41.0	34.0	41.0
50-51	38.2448171867682	40.0	38.0	41.0	34.0	41.0
52-53	38.16911403394381	40.0	37.0	41.0	33.5	41.0
54-55	38.02352142417581	40.0	37.0	41.0	33.0	41.0
56-57	37.88763841544357	40.0	37.0	41.0	33.0	41.0
58-59	37.74161466917874	39.0	36.0	41.0	33.0	41.0
60-61	37.52296218989022	39.0	36.0	41.0	33.0	41.0
62-63	37.30033699252489	39.0	35.0	41.0	32.5	41.0
64-65	37.08127965235974	38.5	35.0	40.0	32.0	41.0
66-67	36.801827727432496	38.0	35.0	40.0	32.0	41.0
68-69	36.551178616289086	37.0	35.0	40.0	32.0	41.0
70-71	36.264432943389764	37.0	35.0	39.5	31.0	41.0
72-73	35.94453385510551	36.5	35.0	39.0	31.0	41.0
74-75	35.6261783597313	36.0	34.5	39.0	31.0	40.0
76-77	35.30123647365198	35.5	34.0	37.5	31.0	40.0
78-79	35.00722355896029	35.0	34.0	37.0	31.0	39.0
80-81	34.7268014193632	35.0	34.0	37.0	31.0	39.0
82-83	34.45401935841173	35.0	34.0	36.0	31.0	38.5
84-85	34.22301105214831	35.0	34.0	36.0	30.5	37.0
86-87	34.027830566588705	35.0	34.0	36.0	30.0	37.0
88-89	33.88677300748667	35.0	34.0	35.0	30.0	36.5
90-91	33.786445802558404	35.0	34.0	35.0	30.0	36.0
92-93	33.70393809120007	35.0	34.0	35.0	30.0	36.0
94-95	33.614519870347905	35.0	34.0	35.0	30.0	36.0
96-97	33.62185303800448	35.0	34.0	35.0	30.0	35.5
98-99	33.70485498597896	35.0	34.0	35.0	31.0	35.0
100	32.918608387568064	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	14.0
20	91.0
21	312.0
22	1130.0
23	3273.0
24	8300.0
25	17340.0
26	32595.0
27	52623.0
28	78844.0
29	108944.0
30	144817.0
31	185682.0
32	239959.0
33	315787.0
34	441121.0
35	648700.0
36	1108544.0
37	2180150.0
38	2960321.0
39	1462585.0
40	44467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.601193510624775	20.411696196860696	14.14629353649267	24.840816756021862
2	14.083508153188829	20.74256020771907	42.2972106284626	22.8767210106295
3	17.47997778309662	26.65706551535224	30.881376124720774	24.981580576830368
4	12.08255850822243	19.11613039900562	38.0081186990551	30.793192393716843
5	13.055564524068899	37.86626910243517	35.535432204616036	13.542734168879896
6	28.890290869723273	38.25923208033317	18.792640723355678	14.05783632658788
7	25.77499390918027	32.8629941722439	22.41645861555792	18.94555330301791
8	23.946909423687902	36.66445985712469	21.534070096827257	17.85456062236015
9	25.08352262604224	16.871351241041395	20.724103900472755	37.32102223244361
10-11	23.719758173000848	26.93517002065845	28.85771499154411	20.4873568147966
12-13	25.060255466437297	24.10053246421441	28.711879225206577	22.127332844141716
14-15	22.151192892824387	25.40625855703354	26.14856066460972	26.29398788553235
16-17	21.776496960222822	28.953269778281403	26.712303514566738	22.557929746929034
18-19	21.90239371740854	27.458292495399377	28.70226346117284	21.937050326019243
20-21	22.528528966380552	26.530190920970103	28.620226384007484	22.321053728641864
22-23	22.077006055106946	27.24439251400133	28.510236939790957	22.168364491100764
24-25	22.099686831593882	27.243761501353426	28.759590330548367	21.896961336504326
26-27	22.001812817694503	27.087518632457897	29.044398072100503	21.866270477747094
28-29	21.537271254549452	26.667957804742485	29.48461877692453	22.310152163783528
30-31	22.12247830808567	26.613648874627906	28.49736565690013	22.766507160386297
32-33	22.380472049400556	26.727213167600954	29.191522380567275	21.700792402431215
34-35	22.24116191932507	27.447166114507926	28.81321781788642	21.49845414828058
36-37	21.795549141792385	27.039807455093793	28.639913171727855	22.52473023138597
38-39	21.72390941260033	26.828876262431788	29.175477847216687	22.271736477751194
40-41	22.03859875589501	26.708463754077066	29.22101877418859	22.03191871583934
42-43	22.51299591373932	26.599107046766623	28.882170674312686	22.00572636518137
44-45	21.93066575477836	27.27704455778759	28.59333874749671	22.198950939937337
46-47	22.724613618494054	26.635480827543713	27.872853257176445	22.767052296785792
48-49	22.359069478061844	26.75295960665453	27.887373964061357	23.000596951222263
50-51	21.969855442881002	26.95261874951127	28.267603650756364	22.809922156851364
52-53	22.254246786412555	26.932380996706858	28.727990905189543	22.085381311691048
54-55	22.224522123379113	26.901192325395023	28.048361965741307	22.82592358548455
56-57	22.82384922204522	27.53219124176906	27.188643811003026	22.455315725182697
58-59	21.93264691305961	28.183056105988648	28.116456238424647	21.767840742527092
60-61	22.588390747171264	28.899700652708738	26.818924617508593	21.692983982611405
62-63	21.88368271277011	29.533554606444532	26.805431007347778	21.777331673437576
64-65	21.963561115756423	29.240737913891817	26.86371786663872	21.931983103713037
66-67	22.17365129255012	28.854251836136548	26.85310231728742	22.11899455402591
68-69	22.229245202551134	28.36887178283163	27.165732076817804	22.236150937799433
70-71	22.401473227160906	27.562105376539208	27.502190437046625	22.534230959253254
72-73	22.512428568624163	27.47249299641622	27.474582539919012	22.540495895040603
74-75	22.315584071232227	27.658292434860815	27.436541767929988	22.589581725976966
76-77	22.46721024604984	27.60684323351449	27.371911363634275	22.55403515680139
78-79	22.429493945085454	27.604812736439715	27.33638450525736	22.62930881321747
80-81	22.415069232195126	27.635452579033178	27.326612856180326	22.622865332591367
82-83	22.503968723935564	27.604585325044788	27.4103572355507	22.481088715468953
84-85	22.550159400462224	27.542034380392106	27.385212431678134	22.522593787467535
86-87	22.38954321123849	27.662026171381417	27.426454714427884	22.521975902952214
88-89	22.48560195435905	27.5962438712962	27.418380793876285	22.499773380468465
90-91	22.504324800670542	27.640662369350053	27.4043804165043	22.450632413475102
92-93	22.53263595981902	27.664910293774653	27.35818036108216	22.444273385324166
94-95	22.375880281215437	27.725494626502993	27.390834074372066	22.5077910179095
96-97	22.447236188503624	27.81657083994415	27.49365657478822	22.242536396764013
98-99	23.53426616249785	29.78298836754399	27.607397244027975	19.075348225930185
100	22.667511188048945	30.777179129930328	21.238429535914968	25.31688014610576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2744.0
1	2135.5
2	2360.0
3	3615.0
4	5151.0
5	8604.5
6	11342.5
7	12068.0
8	11434.5
9	9946.5
10	8268.0
11	7068.5
12	8141.5
13	13885.5
14	26533.5
15	43566.0
16	58547.5
17	66940.5
18	69290.0
19	64476.5
20	56034.0
21	48459.0
22	43709.5
23	45130.0
24	51643.5
25	63327.5
26	80834.0
27	100742.0
28	119443.5
29	139849.5
30	159895.0
31	179122.5
32	202002.0
33	225263.0
34	248049.5
35	268824.5
36	291273.5
37	312399.0
38	327000.5
39	340647.0
40	355295.0
41	366694.5
42	374216.5
43	383727.0
44	393567.5
45	419796.5
46	466680.5
47	485234.0
48	470843.0
49	446620.5
50	420738.5
51	401510.0
52	382749.5
53	361780.0
54	340180.0
55	317774.5
56	292946.0
57	265358.0
58	236280.5
59	207306.0
60	175848.0
61	144199.0
62	114486.0
63	88503.5
64	66585.0
65	49234.5
66	36837.0
67	28305.5
68	21920.5
69	16311.5
70	12061.0
71	8877.5
72	6399.5
73	4534.5
74	3145.5
75	2141.0
76	1464.5
77	994.5
78	679.0
79	477.0
80	325.0
81	204.0
82	146.0
83	121.0
84	104.0
85	87.0
86	71.5
87	56.0
88	34.0
89	18.0
90	10.5
91	7.0
92	7.0
93	5.5
94	3.5
95	2.5
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005101835425152287
3	1.9929044629501123E-5
4	5.9787133888503375E-5
5	0.004942403068116279
6	0.005938855299591335
7	8.968070083275505E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.01057993869119976
26-27	0.002040026720858555
28-29	0.0
30-31	3.349280741960664E-4
32-33	0.0025008786904938095
34-35	4.6763775753515274E-4
36-37	0.0030560306294079454
38-39	2.5416249543651238E-5
40-41	2.5594016651262484E-5
42-43	5.156750253119086E-6
44-45	0.0
46-47	0.0029125796576078603
48-49	0.0
50-51	0.0015435366573073677
52-53	0.0
54-55	4.5199115992422014E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0016450703298551926
64-65	0.001150556464925514
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0027277734485999265
74-75	2.428674534479255E-4
76-77	0.0028659115316380405
78-79	0.0
80-81	0.0
82-83	8.315352433613879E-4
84-85	4.260411077526637E-4
86-87	0.0015603492022987601
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0014088075818923331
96-97	9.279454483554022E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	903.0
20-21	2665.0
22-23	2864.0
24-25	3624.0
26-27	6150.0
28-29	11309.0
30-31	24676.0
32-33	30671.0
34-35	40694.0
36-37	58565.0
38-39	68573.0
40-41	69848.0
42-43	81777.0
44-45	98123.0
46-47	95315.0
48-49	81652.0
50-51	91999.0
52-53	67005.0
54-55	71764.0
56-57	73898.0
58-59	77471.0
60-61	79775.0
62-63	89551.0
64-65	120687.0
66-67	126022.0
68-69	132213.0
70-71	106890.0
72-73	69059.0
74-75	69136.0
76-77	72830.0
78-79	76333.0
80-81	77792.0
82-83	74050.0
84-85	75569.0
86-87	79541.0
88-89	86483.0
90-91	93493.0
92-93	101034.0
94-95	121071.0
96-97	328699.0
98-99	631673.0
100-101	6364157.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.73095970457383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.15931091723894	74.65394669698905
2	7.421738738306289	12.428586144699974
3	1.8537039365652985	4.656372288502767
4	0.6827453834175257	2.2866770474966662
5	0.3293291185609293	1.3787521577883994
6	0.18181866310979994	0.9134310692631686
7	0.10587540969101254	0.6205534763780391
8	0.06591813057125837	0.44155106677302963
9	0.043023020787308595	0.3242122937920061
>10	0.15240622325116812	1.9402403694315347
>50	0.00301135698499775	0.17105885386122002
>100	0.0010773933855301886	0.14882286987807888
>500	2.085409880118776E-5	0.012415010652485896
>1k	2.085403116126841E-5	0.02338065449365562
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.964522314750561E-6
2	0.0	2.9893566944251687E-5	0.0	9.964522314750561E-6	9.964522314750561E-6
3	0.0	4.982261157375281E-5	0.0	9.964522314750561E-6	9.964522314750561E-6
4	0.0	7.971617851800449E-5	0.0	9.964522314750561E-6	1.9929044629501123E-5
5	0.0	7.971617851800449E-5	0.0	9.964522314750561E-6	1.9929044629501123E-5
6	0.0	7.971617851800449E-5	0.0	9.964522314750561E-6	1.9929044629501123E-5
7	0.0	7.971617851800449E-5	0.0	2.9893566944251687E-5	1.9929044629501123E-5
8	0.0	9.964522314750563E-5	0.0	2.9893566944251687E-5	1.9929044629501123E-5
9	0.0	2.391485355540135E-4	0.0	3.9858089259002245E-5	2.9893566944251687E-5
10-11	0.0	2.391485355540135E-4	0.0	3.9858089259002245E-5	2.9893566944251687E-5
12-13	0.0	2.391485355540135E-4	0.0	5.9787133888503375E-5	2.9893566944251687E-5
14-15	0.0	2.790066248130157E-4	0.0	7.473391736062922E-5	4.9822611573752806E-5
16-17	0.0	2.790066248130157E-4	0.0	1.0462748430488091E-4	6.975165620325393E-5
18-19	0.0	2.939534082851416E-4	0.0	1.1957426777700675E-4	8.469843967537978E-5
20-21	0.0	3.1388245291464267E-4	0.0	1.4448557356388315E-4	9.466296199013035E-5
22-23	0.0	3.2882923638676855E-4	0.0	1.4946783472125842E-4	1.0960974546225619E-4
24-25	0.0	3.686873256457708E-4	0.0	1.4946783472125842E-4	1.295387900917573E-4
26-27	0.0	3.886163702752719E-4	0.0	2.142372297671371E-4	1.295387900917573E-4
28-29	0.0	3.886163702752719E-4	0.0	4.78297071108027E-4	1.295387900917573E-4
30-31	0.0	4.683325487932764E-4	0.0	0.0011459200661963146	1.295387900917573E-4
32-33	0.0	4.683325487932764E-4	0.0	0.00239646761669751	1.4946783472125842E-4
34-35	0.0	5.48048727311281E-4	0.0	0.00408545414904773	1.7936140166551013E-4
36-37	0.0	6.377294281440359E-4	0.0	0.006760928390558257	1.7936140166551013E-4
38-39	0.0	6.72605256245663E-4	0.0	0.01080154218918961	1.9430818513763599E-4
40-41	0.0	7.672682182357932E-4	0.0	0.014941801210968466	2.3416627439663823E-4
42-43	0.0	8.071263074947955E-4	0.0	0.01851906472196392	2.391485355540135E-4
44-45	0.0	9.366650975865529E-4	0.0	0.022778898011519784	2.4413079671138877E-4
46-47	0.0	0.0010163812761045574	0.0	0.027442294454823046	2.590775801835146E-4
48-49	0.0	0.0010313280595766832	0.0	0.03213558446507056	2.590775801835146E-4
50-51	9.964522314750561E-6	0.0011558845885110652	0.0	0.03659969046207881	2.640598413408899E-4
52-53	9.964522314750561E-6	0.0011758136331405664	0.0	0.04101397384751331	2.83988885970391E-4
54-55	9.964522314750561E-6	0.0011857781554553168	0.0	0.04508448121308892	3.0391793059989213E-4
56-57	9.964522314750561E-6	0.0011957426777700674	0.0	0.049453924248107045	3.1388245291464267E-4
58-59	9.964522314750561E-6	0.001205707200084818	0.0	0.05396785285668904	3.2384697522939326E-4
60-61	9.964522314750561E-6	0.001240583028186445	0.0	0.05838711850328092	3.3879375870151914E-4
62-63	9.964522314750561E-6	0.0013003701620749484	0.0	0.06238787421265327	3.4875828101626967E-4
64-65	9.964522314750561E-6	0.0014348912133240808	0.0	0.06663774297989439	3.4875828101626967E-4
66-67	9.964522314750561E-6	0.0014498379967962066	0.0	0.07071821486778473	3.587228033310202E-4
68-69	9.964522314750561E-6	0.0014946783472125844	0.0	0.07495313685155372	3.836341091178967E-4
70-71	9.964522314750561E-6	0.0015146073918420855	0.0	0.07851545357907705	4.1850993721952363E-4
72-73	9.964522314750561E-6	0.0015146073918420855	0.0	0.08122082138753184	4.78297071108027E-4
74-75	9.964522314750561E-6	0.0015494832199437125	0.0	0.08329344202899994	4.8826159342277753E-4
76-77	9.964522314750561E-6	0.0015993058315174652	0.0	0.08539097397625495	4.8826159342277753E-4
78-79	9.964522314750561E-6	0.0016242171373043416	0.0	0.08670130866064464	4.8826159342277753E-4
80-81	9.964522314750561E-6	0.0016242171373043416	0.0	0.08711981859786416	5.081906380522787E-4
82-83	9.964522314750561E-6	0.0016242171373043416	0.0	0.08737889617804767	5.380842049965304E-4
84-85	9.964522314750561E-6	0.0016242171373043416	0.0	0.08745861235656568	5.380842049965304E-4
86-87	9.964522314750561E-6	0.0016441461819338427	0.0	0.08751839949045419	5.430664661539057E-4
88	9.964522314750561E-6	0.0017437914050813484	0.0	0.08753832853508368	5.48048727311281E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	17000	0.0	59.594807	1
GGTATCA	13820	0.0	52.515266	1
TATACAC	21610	0.0	48.59723	3
TTATACA	22170	0.0	46.154198	2
GTATCAA	26555	0.0	38.86649	1
TACACAT	30220	0.0	34.667587	5
ACACATC	31655	0.0	32.334652	6
ACATCTC	32415	0.0	31.457825	8
TCAACGC	32955	0.0	30.843016	4
ATCAACG	33355	0.0	30.485788	3
ATACACA	35300	0.0	30.467476	4
CACATCT	33645	0.0	30.295395	7
TATCAAC	33995	0.0	30.186562	2
CAACGCA	33730	0.0	30.146858	5
AACGCAG	34665	0.0	29.320093	6
CATCTCC	35645	0.0	28.548069	9
ACGCAGA	39770	0.0	25.565935	7
CGCAGAG	40450	0.0	25.115166	8
GTGGTAT	6475	0.0	23.590326	1
TGGTATC	6055	0.0	23.414793	2
>>END_MODULE